ID pCAGGS-FLAGmA20f1 standard; circular DNA; ; 6178 BP. DE LMBP 4121. CC Accession number: LMBP 4121 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 9-JUN-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 13..36 FT /vntifkey="22" FT /label=FLAG\epitope FT promoter complement(1990..2072) FT /vntifkey="30" FT /label=lac\promoter FT terminator 1464..1555 FT /vntifkey="43" FT /label=3'\UTR\of\ß-globin,\incl.\polyA FT promoter 4451..4828 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT CDS complement(3451..4311) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin 2214..2289 FT /vntifkey="33" FT /label=SV40\ori FT rep_origin complement(2509..2910) FT /vntifkey="33" FT /label=pMB1\ori FT intron 5203..6127 FT /vntifkey="15" FT /label=intron\AG\promoter FT misc_feature 1556..1912 FT /vntifkey="21" FT /label=3'\flanking\region\of\rabbit\ß-globin FT terminator 2320..2394 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(2320..2394) FT /vntifkey="43" FT /label=SV40\polyA\late FT promoter 4834..6175 FT /vntifkey="29" FT /label=AG\promoter FT CDS 46..1449 FT /vntifkey="4" FT /label=mA20\(1-468) SQ Sequence 6178 BP; 1406 A; 1616 C; 1661 G; 1495 t; ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt 60 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc 120 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga 180 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac 240 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg 300 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg 360 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc 420 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa 480 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat 540 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga 600 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga 660 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct 720 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga 780 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac 840 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac 900 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag 960 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc 1020 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac 1080 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga 1140 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata 1200 aaatgtgaga cacccaactg tcctttcttc atgtccgtga acactcagcc tttatgccac 1260 gaatgctcag agaggcgcca aaagaatcag agcaagctcc caaagctgaa ctcgaagcta 1320 ggccctgaag gactcccagg cgtgggactt ggctcctcaa actggagccc cgaggaaacc 1380 gctggaggac ctcattcagc cccacccaca gcacccagcc tttttctctt cagtgagacc 1440 actgcaatgt aagtgtcgac gaagatcttt ttccctctgc caaaaattat ggggacatca 1500 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag 1560 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa 1620 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca 1680 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt 1740 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt 1800 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc 1860 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc 1920 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 1980 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg ggtgcctaat 2040 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 2100 tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 2160 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 2220 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 2280 aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat 2340 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 2400 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc 2460 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 2520 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 2580 caaaggcggt aatacggtta tccacagaat cagggataac gcaggaaaga acatgtgagc 2640 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 2700 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 2760 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 2820 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 2880 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 2940 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 3000 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 3060 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 3120 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 3180 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 3240 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 3300 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 3360 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 3420 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 3480 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 3540 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 3600 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 3660 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 3720 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 3780 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 3840 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 3900 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 3960 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 4020 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 4080 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 4140 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 4200 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 4260 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 4320 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 4380 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 4440 tgggtcgatc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta 4500 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc 4560 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg 4620 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg 4680 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa 4740 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac 4800 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact 4860 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt 4920 tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc ggggcggggc 4980 gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag cggcgcgctc 5040 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg 5100 cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc cgccgcctcg 5160 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc 5220 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg 5280 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg 5340 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc 5400 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc 5460 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg 5520 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc 5580 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt 5640 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg 5700 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg gcggcccccg 5760 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg 5820 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg 5880 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat 5940 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg 6000 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt 6060 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt 6120 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaa 6178 //