ID pCAGGS-FLAGmA20f2 standard; circular DNA; ; 6694 BP. DE LMBP 4122. CC Accession number: LMBP 4122 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 9-JUN-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 13..36 FT /vntifkey="22" FT /label=FLAG\epitope FT promoter complement(2506..2588) FT /vntifkey="30" FT /label=lac\promoter FT terminator 1980..2071 FT /vntifkey="43" FT /label=3'\UTR\of\ß-globin,\incl.\polyA FT promoter 4967..5344 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT CDS complement(3967..4827) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin 2730..2805 FT /vntifkey="33" FT /label=SV40\ori FT rep_origin complement(3025..3426) FT /vntifkey="33" FT /label=pMB1\ori FT intron 5719..6643 FT /vntifkey="15" FT /label=intron\AG\promoter FT CDS 46..1965 FT /vntifkey="4" FT /label=mA20\(1-640) FT misc_feature 2072..2428 FT /vntifkey="21" FT /label=3'\flanking\region\of\rabbit\ß-globin FT terminator 2836..2910 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(2836..2910) FT /vntifkey="43" FT /label=SV40\polyA\late FT promoter 5350..6691 FT /vntifkey="29" FT /label=AG\promoter SQ Sequence 6694 BP; 1531 A; 1774 C; 1778 G; 1611 t; ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt 60 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc 120 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga 180 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac 240 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg 300 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg 360 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc 420 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa 480 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat 540 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga 600 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga 660 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct 720 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga 780 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac 840 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac 900 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag 960 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc 1020 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac 1080 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga 1140 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata 1200 aaatgtgaga cacccaactg tcctttcttc atgtccgtga acactcagcc tttatgccac 1260 gaatgctcag agaggcgcca aaagaatcag agcaagctcc caaagctgaa ctcgaagcta 1320 ggccctgaag gactcccagg cgtgggactt ggctcctcaa actggagccc cgaggaaacc 1380 gctggaggac ctcattcagc cccacccaca gcacccagcc tttttctctt cagtgagacc 1440 actgcaatga agtgcaggag tcctgggtgc ccttttactt tgaatgtgca gcataatgga 1500 ttctgtgagc gttgccacgc ccggcagatt aatgccagcc acaccgcaga ccctggaaag 1560 tgccaagcct gccttcagga tgtcactcgg acctttaatg gcatctgcag tacctgtttc 1620 aaaaggacta cagcagagcc cagctccagc ctcacttcca gtatccctgc ctcctgtcac 1680 caacgctcca agtctgaccc ctcacaactc atccaaagtc tcactccaca ctcttgccac 1740 cggactggaa atgtctctcc ttctggctgc ctctcccagg ctgcacggac tccaggagac 1800 agagcaggga caagcaagtg caggaaagct ggctgcatgt attttgggac tccagaaaac 1860 aagggctttt gcactctatg tttcatcgaa tacagagaaa ataagcagtc tgttactgcc 1920 tctgcgaaag ctggttcccc ggcccccagg ttccagaaca atgtctaagt gtcgacgaag 1980 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt 2040 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct 2100 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt 2160 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag 2220 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga 2280 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa 2340 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca 2400 tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca 2460 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 2520 gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc acattaattg 2580 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct 2640 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 2700 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 2760 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 2820 cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc 2880 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 2940 ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg 3000 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 3060 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 3120 cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 3180 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 3240 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 3300 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 3360 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 3420 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 3480 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 3540 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 3600 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 3660 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 3720 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 3780 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 3840 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 3900 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 3960 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 4020 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 4080 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 4140 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 4200 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 4260 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 4320 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 4380 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 4440 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 4500 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 4560 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 4620 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 4680 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 4740 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 4800 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 4860 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 4920 aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgatcgaca ttgattattg 4980 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc 5040 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca 5100 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt 5160 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg 5220 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 5280 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 5340 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca 5400 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg 5460 gggggggggc cccccccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg 5520 aaaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg 5580 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgttgcgcgc 5640 tgccttcccc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg 5700 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag 5760 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc 5820 cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg 5880 gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg 5940 gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg 6000 tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga 6060 gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt 6120 gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc 6180 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc 6240 ggggagggct cgggggaagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc 6300 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt 6360 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg 6420 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc 6480 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg 6540 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag 6600 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct 6660 ggttattgtg ctgtctcatc attttggcaa agaa 6694 //