LOCUS       pCAGGS-FLAGmA20d3 6742 bp dsDNACircular
DEFINITION  LMBP 4124.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     misc_difference 13..36
                     /label=FLAG-tag
     promoter        complement(2554..2636)
                     /label=lac\promoter
     terminator      2028..2119
                     /label=ß-globin\polyA
     promoter        5015..5392
                     /label=hCMV\enhancer
     CDS             complement(4015..4875)
                     /label=AMP
     rep_origin      2778..2853
                     /label=SV40\ori
     rep_origin      complement(3073..3474)
                     /label=pMB1\ori
     intron          5767..6691
                     /label=intron\AG\promoter
     promoter        5398..6739
                     /label=AG\promoter
     CDS             46..2016
                     /label=mA20\(d386-504)
     misc_feature    2120..2476
                     /label=3'\FR\of\ß-globin
     terminator      2884..2958
                     /label=SV40\polyA\early
     terminator      complement(2884..2958)
                     /label=SV40\polyA\late
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt 
       61 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc 
      121 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga 
      181 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac 
      241 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg 
      301 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg 
      361 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc 
      421 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa 
      481 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat 
      541 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga 
      601 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga 
      661 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct 
      721 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga 
      781 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac 
      841 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac 
      901 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag 
      961 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc 
     1021 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac 
     1081 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga 
     1141 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata 
     1201 aagtgccaag cctgccttca ggatgtcact cggaccttta atggcatctg cagtacctgt 
     1261 ttcaaaagga ctacagcaga gcccagctcc agcctcactt ccagtatccc tgcctcctgt 
     1321 caccaacgct ccaagtctga cccctcacaa ctcatccaaa gtctcactcc acactcttgc 
     1381 caccggactg gaaatgtctc tccttctggc tgcctctccc aggctgcacg gactccagga 
     1441 gacagagcag ggacaagcaa gtgcaggaaa gctggctgca tgtattttgg gactccagaa 
     1501 aacaagggct tttgcactct atgtttcatc gaatacagag aaaataagca gtctgttact 
     1561 gcctctgcga aagctggttc cccggccccc aggttccaga acaatgtccc gtgcctgggc 
     1621 agggagtgcg gcacactcgg aagcaccatg tttgaagggt actgtcagaa gtgtttcatc 
     1681 gaagctcaga accagagatt ccatgaagca agaagaacgg aagaacagct gagatcaagc 
     1741 cagcatagag acatgcctcg aactacacag gtagcctcaa ggccgaaatg tgcccgggcc 
     1801 tcctgcaaga acattctggc ctgtcgcagt gaggaactct gtatggagtg ccagcaccta 
     1861 agccaacgag taggttctgt ggcccaccgg ggtgagccca cgcctgaaga gccccctaaa 
     1921 cagcgctgcc gggcccctgc ttgtgatcac tttggcaatg ccaagtgtaa tggttactgc 
     1981 aatgagtgct accagttcaa gcagatgtat ggctaagtgt cgacgaagat ctttttccct 
     2041 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa 
     2101 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga 
     2161 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa 
     2221 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata 
     2281 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga 
     2341 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac 
     2401 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc 
     2461 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg 
     2521 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 
     2581 aagtgtaaag cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
     2641 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc 
     2701 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 
     2761 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 
     2821 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 
     2881 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 
     2941 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 
     3001 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 
     3061 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 
     3121 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga 
     3181 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 
     3241 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 
     3301 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 
     3361 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 
     3421 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 
     3481 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 
     3541 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 
     3601 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 
     3661 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 
     3721 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
     3781 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
     3841 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 
     3901 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 
     3961 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 
     4021 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
     4081 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 
     4141 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 
     4201 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 
     4261 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 
     4321 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 
     4381 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 
     4441 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 
     4501 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 
     4561 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 
     4621 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 
     4681 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 
     4741 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 
     4801 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 
     4861 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 
     4921 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 
     4981 cacatttccc cgaaaagtgc cacctgggtc gatcgacatt gattattgac tagttattaa 
     5041 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa 
     5101 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata 
     5161 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag 
     5221 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc 
     5281 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta 
     5341 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag 
     5401 gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg 
     5461 tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggccc 
     5521 cccccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg 
     5581 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg 
     5641 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc 
     5701 gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc 
     5761 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa 
     5821 tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct 
     5881 ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg 
     5941 tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg 
     6001 ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc 
     6061 tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg 
     6121 ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc 
     6181 ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg 
     6241 gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg 
     6301 ggggaagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag 
     6361 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg 
     6421 tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg 
     6481 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg 
     6541 tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg 
     6601 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa 
     6661 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct 
     6721 gtctcatcat tttggcaaag aa 
//
