LOCUS       pCAGGS-FLAGmA20d4 6841 bp dsDNACircular
DEFINITION  LMBP 4125.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     misc_difference 13..36
                     /label=FLAG-tag
     promoter        complement(2653..2735)
                     /label=lac\promoter
     terminator      2127..2218
                     /label=ß-globin\polyA
     promoter        5114..5491
                     /label=hCMV\enhancer
     CDS             complement(4114..4974)
                     /label=AMP
     rep_origin      2877..2952
                     /label=SV40\ori
     rep_origin      complement(3172..3573)
                     /label=pMB1\ori
     intron          5866..6790
                     /label=intron\AG\promoter
     promoter        5497..6838
                     /label=AG\promoter
     CDS             46..2115
                     /label=mA20\(d505-590)
     misc_feature    2219..2575
                     /label=3'\FR\of\ß-globin
     terminator      2983..3057
                     /label=SV40\polyA\early
     terminator      complement(2983..3057)
                     /label=SV40\polyA\late
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt 
       61 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc 
      121 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga 
      181 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac 
      241 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg 
      301 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg 
      361 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc 
      421 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa 
      481 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat 
      541 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga 
      601 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga 
      661 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct 
      721 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga 
      781 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac 
      841 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac 
      901 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag 
      961 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc 
     1021 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac 
     1081 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga 
     1141 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata 
     1201 aaatgtgaga cacccaactg tcctttcttc atgtccgtga acactcagcc tttatgccac 
     1261 gaatgctcag agaggcgcca aaagaatcag agcaagctcc caaagctgaa ctcgaagcta 
     1321 ggccctgaag gactcccagg cgtgggactt ggctcctcaa actggagccc cgaggaaacc 
     1381 gctggaggac ctcattcagc cccacccaca gcacccagcc tttttctctt cagtgagacc 
     1441 actgcaatga agtgcaggag tcctgggtgc ccttttactt tgaatgtgca gcataatgga 
     1501 ttctgtgagc gttgccacgc ccggcagatt aatgccagcc acaccgcaga ccctggaaag 
     1561 tgcaggaaag ctggctgcat gtattttggg actccagaaa acaagggctt ttgcactcta 
     1621 tgtttcatcg aatacagaga aaataagcag tctgttactg cctctgcgaa agctggttcc 
     1681 ccggccccca ggttccagaa caatgtcccg tgcctgggca gggagtgcgg cacactcgga 
     1741 agcaccatgt ttgaagggta ctgtcagaag tgtttcatcg aagctcagaa ccagagattc 
     1801 catgaagcaa gaagaacgga agaacagctg agatcaagcc agcatagaga catgcctcga 
     1861 actacacagg tagcctcaag gccgaaatgt gcccgggcct cctgcaagaa cattctggcc 
     1921 tgtcgcagtg aggaactctg tatggagtgc cagcacctaa gccaacgagt aggttctgtg 
     1981 gcccaccggg gtgagcccac gcctgaagag ccccctaaac agcgctgccg ggcccctgct 
     2041 tgtgatcact ttggcaatgc caagtgtaat ggttactgca atgagtgcta ccagttcaag 
     2101 cagatgtatg gctaagtgtc gacgaagatc tttttccctc tgccaaaaat tatggggaca 
     2161 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa 
     2221 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt 
     2281 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg 
     2341 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc 
     2401 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg 
     2461 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt 
     2521 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg 
     2581 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 
     2641 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctgggtgcct 
     2701 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 
     2761 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac 
     2821 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 
     2881 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 
     2941 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct 
     3001 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 
     3061 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc 
     3121 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 
     3181 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 
     3241 actcaaaggc ggtaatacgg ttatccacag aatcagggat aacgcaggaa agaacatgtg 
     3301 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 
     3361 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 
     3421 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 
     3481 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 
     3541 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 
     3601 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 
     3661 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 
     3721 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 
     3781 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 
     3841 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 
     3901 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 
     3961 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 
     4021 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 
     4081 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 
     4141 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 
     4201 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
     4261 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
     4321 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
     4381 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 
     4441 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
     4501 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
     4561 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
     4621 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
     4681 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 
     4741 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
     4801 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
     4861 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
     4921 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
     4981 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
     5041 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 
     5101 acctgggtcg atcgacattg attattgact agttattaat agtaatcaat tacggggtca 
     5161 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 
     5221 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 
     5281 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 
     5341 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 
     5401 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 
     5461 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc 
     5521 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta 
     5581 ttttgtgcag cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg 
     5641 ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg 
     5701 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc 
     5761 gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc 
     5821 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac 
     5881 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg 
     5941 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg 
     6001 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc 
     6061 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg 
     6121 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc 
     6181 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa 
     6241 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc 
     6301 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg 
     6361 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc 
     6421 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc 
     6481 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag 
     6541 gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga 
     6601 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc 
     6661 tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg 
     6721 cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt 
     6781 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga 
     6841 a 
//
