ID pCAGGS-FLAGmA20f8 standard; circular DNA; ; 5332 BP. DE LMBP 4127. CC Accession number: LMBP 4127 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 20-MAY-2011 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter complement(1144..1226) FT /vntifkey="30" FT /label=lac\promoter FT terminator 618..709 FT /vntifkey="43" FT /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA FT promoter 3605..3982 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT CDS complement(2605..3465) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(1663..2064) FT /vntifkey="33" FT /label=pMB1\ori FT intron 4357..5281 FT /vntifkey="15" FT /label=intron\AG\promoter FT rep_origin 1368..1443 FT /vntifkey="33" FT /label=SV40\ori FT promoter 3988..5329 FT /vntifkey="29" FT /label=AG\promoter FT misc_feature 710..1066 FT /vntifkey="21" FT /label=3'\flanking\region\of\rabbit\ß-globin FT terminator 1474..1548 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(1474..1548) FT /vntifkey="43" FT /label=SV40\polyA\late FT CDS 49..606 FT /vntifkey="4" FT /label=mA20\(591-775) FT misc_marker 13..36 FT /vntifkey="22" FT /label=FLAG\epitope SQ Sequence 5332 BP; 1151 A; 1405 C; 1483 G; 1293 t; ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatgaa gtgcaggaaa 60 gctggctgca tgtattttgg gactccagaa aacaagggct tttgcactct atgtttcatc 120 gaatacagag aaaataagca gtctgttact gcctctgcga aagctggttc cccggccccc 180 aggttccaga acaatgtccc gtgcctgggc agggagtgcg gcacactcgg aagcaccatg 240 tttgaagggt actgtcagaa gtgtttcatc gaagctcaga accagagatt ccatgaagca 300 agaagaacgg aagaacagct gagatcaagc cagcatagag acatgcctcg aactacacag 360 gtagcctcaa ggccgaaatg tgcccgggcc tcctgcaaga acattctggc ctgtcgcagt 420 gaggaactct gtatggagtg ccagcaccta agccaacgag taggttctgt ggcccaccgg 480 ggtgagccca cgcctgaaga gccccctaaa cagcgctgcc gggcccctgc ttgtgatcac 540 tttggcaatg ccaagtgtaa tggttactgc aatgagtgct accagttcaa gcagatgtat 600 ggctaagtgt cgacgaagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc 660 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt 720 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc 780 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca 840 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat 900 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt 960 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct 1020 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc 1080 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 1140 attccacaca acatacgagc cggaagcata aagtgtaaag cctgggtgcc taatgagtga 1200 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 1260 gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 1320 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 1380 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 1440 cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt 1500 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 1560 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat 1620 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 1680 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 1740 cggtaatacg gttatccaca gaatcaggga taacgcagga aagaacatgt gagcaaaagg 1800 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 1860 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 1920 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 1980 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 2040 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 2100 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 2160 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 2220 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 2280 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 2340 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 2400 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 2460 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 2520 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 2580 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 2640 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 2700 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 2760 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 2820 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 2880 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 2940 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 3000 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 3060 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 3120 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 3180 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 3240 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 3300 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 3360 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 3420 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 3480 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 3540 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc 3600 gatcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat 3660 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg 3720 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata 3780 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta 3840 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 3900 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 3960 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc 4020 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 4080 gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg gggcgagggg 4140 cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc gctccgaaag 4200 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg 4260 gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc 4320 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct 4380 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt 4440 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg 4500 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag 4560 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc 4620 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt 4680 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg 4740 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg 4800 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg 4860 gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc cccggagcgc 4920 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag 4980 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg 5040 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg 5100 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg 5160 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg 5220 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca 5280 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aa 5332 //