LOCUS pPIC9-TSE 10878 bp DNA circular 17-DEC-2012 DEFINITION LMBP 4166. SOURCE ORGANISM COMMENT Accession number: LMBP 4166 (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) Last modified: 17-DEC-2012 COMMENT VNTNAME|pPIC9-TSE| COMMENT VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| COMMENT VNTOAUTHORTEL|0032-9-33-13-843| COMMENT VNTOAUTHOREML|bccm.genecorner@UGent.be| COMMENT VNTOAUTHORWWW|www.genecorner.ugent.be| COMMENT VNTOAUTHORAD1|Ghent University| COMMENT VNTOAUTHORAD2|Technologiepark 927| COMMENT VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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/note="origin plasmid" CDS complement(9708..10568) /vntifkey="4" /label=ampicillin\resistance promoter 7..772 /vntifkey="29" /label=AOX1\promoter CDS 1219..3141 /vntifkey="4" /label=mature\TS /note="Trypanosoma cruzi trans-sialidase gene; mature sequence" CDS 3199..4090 /vntifkey="4" /label=fd\g3\fragment /note="fragment of phage fd gene 3" BASE COUNT 2788 a 2568 c 2663 g 2859 t ORIGIN 1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag 61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt 121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc 181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta 241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta 301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg 361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct 421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg 481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt 541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct 601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct 661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact 721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat 781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt 841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga 901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct 961 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact 1021 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta 1081 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg 1141 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgagaaa 1201 agagaggctg aagcttacgt gctggcaccc ggatcgagcc gagttgagct gtttaagcgt 1261 aagaattcga cggtgccgtt tgaagacaag gccggcaaag tcaccgagcg ggttgtccac 1321 tcgttccgcc tccccgccct tgttaatgtg gacggggtga tggttgccat cgcggacgct 1381 cgctacgaca catccaatga caactccctc attgatacgg tggcgaagta cagcgtggac 1441 gatggggaga cgtgggagac ccaaattgcc atcaagaaca gccgtgtatc gtctgtttct 1501 cgtgtggtgg atcccaccgt gattgtgaag ggcaacaagc tttacgtcct ggttggaagc 1561 tactatagtt cgagaagcta ctggtcgtcg catggtgatg cgagagactg ggatattctg 1621 cttgccgttg gtgaggtcac gaagtccatt gcgggcggca agataactgc gagtatcaaa 1681 tgggggagcc ccgtgtcact gaagaagttt tttccggcag aaatggaagg catgcacaca 1741 aatcaatttc ttggcggcgc gggtgttgcc attgtggcgt ccaacgggaa tcttgtgtac 1801 cctgtgcagg ttacgaacaa aaggaagcaa gttttctcca agatcttcta ctcggaagat 1861 gatgggaaga cgtggaagtt tgggaagggt aggagcgatt ttggctgctc tgaacctgtg 1921 gcccttgagt gggaggggaa gctcatcata aacacccgag ttgactggaa acgccgtctg 1981 gtgtacgagt ccagtgacat ggggaatacg tgggtggagg ctgtcggcac gctctcgcgt 2041 gtgtggggcc cctcaccaaa atcggaccat cccggcagtc agagcagctt cactgccgtg 2101 accatcgaag gaatgcgtgt gatgctcttc acacacccgc tgaattttaa gggaaggtgg 2161 ctgcgcgacc gactgaacct ctggctgacg gataaccagc gcatttataa cgttgggcaa 2221 gtatccattg gtgatgaaaa ttccgcctac agctccgtcc tgtacaagga tgataagctg 2281 tactgtttgc atgagatcaa cacggacgag gtgtacagcc ttgtttttgc acgcctggtt 2341 ggcgagctac ggatcattaa atcagtgctg cgatcctgga agaattggga cagccacctg 2401 tccagcattt gcacccctgc tgatccagcc gcttcgtcgt cagagagtgg ttgtggtccc 2461 gctgtcacca cggttggtct tgttggcttt ttgtccggca atgcctccca aaacgtatgg 2521 gaggatgcgt accgctgcgt caacgcaagc acggcaaatg cggagagggt tcggaacggt 2581 ttgaagtttg cgggggttgg cggaggggcg ctttggccgg tgagccagca ggggcagaat 2641 cagcggtatc gttttgcaaa ccacgcgttc acgctggtgg cgtcggtgac gattcacgag 2701 gctccgaggg ccgcgagtcc tttgctgggt gcgagcctgg actcttctgg tggcaaaaaa 2761 ctcctggggc tctcgtacga cgagaagcac cagtggcagc caatatacgg atcaacgccg 2821 gtgacgccga cgggatcgtg ggagacgggt aagaggtacc acgtggttct tacggtggcg 2881 aataaaattg gctccgtgta cattgatgga gaacttctgg agggttcagg acagaccgtt 2941 gtgccagacg ggaggacgcc tgacatctcc cacttctacg ttggcgggta tggaaggagt 3001 gatatgccaa ccataagcca cgtgacggtg aataatgttc ttctttacaa ccgacagctg 3061 aataccgagg agatcaggac cttgttcttg agccaggacc tgattggcac ggaagcacac 3121 atggacagca gcagcgacac gaaggaattg ggtgcgccgg tgccgtatcc ggatccgctg 3181 gaaccgcgtg ccgcatagac tgttgaaagt tgtttagcaa aacctcatac agaaaattca 3241 tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggctgt 3301 ctgtggaatg ctacaggcgt tgtggtttgt actggtgacg aaactcagtg ttacggtaca 3361 tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt 3421 tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct 3481 attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa 3541 aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt 3601 cagaataata ggttccgaaa taggcagggt gcattaactg tttatacggg cactgttact 3661 caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg 3721 tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgag 3781 gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat 3841 gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggcgg ctctgagggt 3901 ggcggttctg agggtggcgg ctctgagggt ggcggttccg gtggcggctc cggttccggt 3961 gattttgatt atgaaaaaat ggcaaacgct aataaggggg ctatgaccga aaatgccgat 4021 gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt 4081 gctgctatcg ggccgcgaat taattcgcct tagacatgac tgttcctcag ttcaagttgg 4141 gcacttacga gaagaccggt cttgctagat tctaatcaag aggatgtcag aatgccattt 4201 gcctgagaga tgcaggcttc atttttgata cttttttatt tgtaacctat atagtatagg 4261 attttttttg tcattttgtt tcttctcgta cgagcttgct cctgatcagc ctatctcgca 4321 gctgatgaat atcttgtggt aggggtttgg gaaaatcatt cgagtttgat gtttttcttg 4381 gtatttccca ctcctcttca gagtacagaa gattaagtga gaagttcgtt tgtgcaagct 4441 tatcgataag ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc 4501 gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt 4561 aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga 4621 cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg 4681 cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc 4741 gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatcta 4801 tcgaatctaa atgtaagtta aaatctctaa ataattaaat aagtcccagt ttctccatac 4861 gaaccttaac agcattgcgg tgagcatcta gaccttcaac agcagccaga tccatcactg 4921 cttggccaat atgtttcagt ccctcaggag ttacgtcttg tgaagtgatg aacttctgga 4981 aggttgcagt gttaactccg ctgtattgac gggcatatcc gtacgttggc aaagtgtggt 5041 tggtaccgga ggagtaatct ccacaactct ctggagagta ggcaccaaca aacacagatc 5101 cagcgtgttg tacttgatca acataagaag aagcattctc gatttgcagg atcaagtgtt 5161 caggagcgta ctgattggac atttccaaag cctgctcgta ggttgcaacc gatagggttg 5221 tagagtgtgc aatacacttg cgtacaattt caacccttgg caactgcaca gcttggttgt 5281 gaacagcatc ttcaattctg gcaagctcct tgtctgtcat atcgacagcc aacagaatca 5341 cctgggaatc aataccatgt tcagcttgag acagaaggtc tgaggcaacg aaatctggat 5401 cagcgtattt atcagcaata actagaactt cagaaggccc agcaggcatg tcaatactac 5461 acagggctga tgtgtcattt tgaaccatca tcttggcagc agtaacgaac tggtttcctg 5521 gaccaaatat tttgtcacac ttaggaacag tttctgttcc gtaagccata gcagctactg 5581 cctgggcgcc tcctgctagc acgatacact tagcaccaac cttgtgggca acgtagatga 5641 cttctggggt aagggtacca tccttcttag gtggagatgc aaaaacaatt tctttgcaac 5701 cagcaacttt ggcaggaaca cccagcatca gggaagtgga aggcagaatt gcggttccac 5761 caggaatata gaggccaact ttctcaatag gtcttgcaaa acgagagcag actacaccag 5821 ggcaagtctc aacttgcaac gtctccgtta gttgagcttc atggaatttc ctgacgttat 5881 ctatagagag atcaatggct ctcttaacgt tatctggcaa ttgcataagt tcctctggga 5941 aaggagcttc taacacaggt gtcttcaaag cgactccatc aaacttggca gttagttcta 6001 aaagggcttt gtcaccattt tgacgaacat tgtcgacaat tggtttgact aattccataa 6061 tctgttccgt tttctggata ggacgacgaa gggcatcttc aatttcttgt gaggaggcct 6121 tagaaacgtc aattttgcac aattcaatac gaccttcaga agggacttct ttaggtttgg 6181 attcttcttt aggttgttcc ttggtgtatc ctggcttggc atctcctttc cttctagtga 6241 cctttaggga cttcatatcc aggtttctct ccacctcgtc caacgtcaca ccgtacttgg 6301 cacatctaac taatgcaaaa taaaataagt cagcacattc ccaggctata tcttccttgg 6361 atttagcttc tgcaagttca tcagcttcct ccctaatttt agcgttcaac aaaacttcgt 6421 cgtcaaataa ccgtttggta taagaacctt ctggagcatt gctcttacga tcccacaagg 6481 tggcttccat ggctctaaga ccctttgatt ggccaaaaca ggaagtgcgt tccaagtgac 6541 agaaaccaac acctgtttgt tcaaccacaa atttcaagca gtctccatca caatccaatt 6601 cgatacccag caacttttga gttgctccag atgtagcacc tttataccac aaaccgtgac 6661 gacgagattg gtagactcca gtttgtgtcc ttatagcctc cggaatagac tttttggacg 6721 agtacaccag gcccaacgag taattagaag agtcagccac caaagtagtg aatagaccat 6781 cggggcggtc agtagtcaaa gacgccaaca aaatttcact gacagggaac tttttgacat 6841 cttcagaaag ttcgtattca gtagtcaatt gccgagcatc aataatgggg attataccag 6901 aagcaacagt ggaagtcaca tctaccaact ttgcggtctc agaaaaagca taaacagttc 6961 tactaccgcc attagtgaaa cttttcaaat cgcccagtgg agaagaaaaa ggcacagcga 7021 tactagcatt agcgggcaag gatgcaactt tatcaaccag ggtcctatag ataaccctag 7081 cgcctgggat catcctttgg acaactcttt ctgccaaatc taggtccaaa atcacttcat 7141 tgataccatt attgtacaac ttgagcaagt tgtcgatcag ctcctcaaat tggtcctctg 7201 taacggatga ctcaacttgc acattaactt gaagctcagt cgattgagtg aacttgatca 7261 ggttgtgcag ctggtcagca gcatagggaa acacggcttt tcctaccaaa ctcaaggaat 7321 tatcaaactc tgcaacactt gcgtatgcag gtagcaaggg aaatgtcata cttgaagtcg 7381 gacagtgagt gtagtcttga gaaattctga agccgtattt ttattatcag tgagtcagtc 7441 atcaggagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg 7501 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc 7561 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg 7621 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac 7681 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagta tctatgattg 7741 gaagtatggg aatggtgata cccgcattct tcagtgtctt gaggtctcct atcagattat 7801 gcccaactaa agcaaccgga ggaggagatt tcatggtaaa tttctctgac ttttggtcat 7861 cagtagactc gaactgtgag actatctcgg ttatgacagc agaaatgtcc ttcttggaga 7921 cagtaaatga agtcccacca ataaagaaat ccttgttatc aggaacaaac ttcttgtttc 7981 gaactttttc ggtgccttga actataaaat gtagagtgga tatgtcgggt aggaatggag 8041 cgggcaaatg cttaccttct ggaccttcaa gaggtatgta gggtttgtag atactgatgc 8101 caacttcagt gacaacgttg ctatttcgtt caaaccattc cgaatccaga gaaatcaaag 8161 ttgtttgtct actattgatc caagccagtg cggtcttgaa actgacaata gtgtgctcgt 8221 gttttgaggt catctttgta tgaataaatc tagtctttga tctaaataat cttgacgagc 8281 caaggcgata aatacccaaa tctaaaactc ttttaaaacg ttaaaaggac aagtatgtct 8341 gcctgtatta aaccccaaat cagctcgtag tctgatcctc atcaacttga ggggcactat 8401 cttgttttag agaaatttgc ggagatgcga tatcgagaaa aaggtacgct gattttaaac 8461 gtgaaattta tctcaagatc tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg 8521 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 8581 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc 8641 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg 8701 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 8761 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 8821 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 8881 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 8941 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 9001 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 9061 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 9121 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc 9181 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 9241 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 9301 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 9361 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 9421 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 9481 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 9541 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 9601 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 9661 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 9721 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 9781 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 9841 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 9901 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 9961 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 10021 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 10081 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 10141 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 10201 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 10261 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 10321 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 10381 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 10441 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 10501 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 10561 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 10621 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 10681 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa 10741 aataggcgta tcacgaggcc ctttcgtctt caagaattaa ttctcatgtt tgacagctta 10801 tcatcgataa gctgactcat gttggtattg tgaaatagac gcagatcggg aacactgaaa 10861 aataacagtt attattcg //