ID pPIC9-TSE standard; circular DNA; ; 10878 BP. DE LMBP 4166. CC Accession number: LMBP 4166 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 17-DEC-2012 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_feature 773..937 FT /vntifkey="21" FT /label=5'\UTR\of\AOX1 FT CDS 949..1191 FT /vntifkey="4" FT /label=ppMF FT /note="S. cerevisiae alpha mating factor 1 prepro sequence" FT misc_marker 3151..3189 FT /vntifkey="22" FT /label=E-tag FT /note="E-tag; C-terminal" FT terminator 4176..4435 FT /vntifkey="43" FT /label=AOX1\terminator FT CDS complement(4838..7369) FT /vntifkey="4" FT /label=Pichia\HIS4 FT misc_feature 7724..8481 FT /vntifkey="21" FT /label=3'\flanking\region\of\AOX1 FT rep_origin complement(8765..9167) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(9708..10568) FT /vntifkey="4" FT /label=ampicillin\resistance FT promoter 7..772 FT /vntifkey="29" FT /label=AOX1\promoter FT CDS 1219..3141 FT /vntifkey="4" FT /label=mature\TS FT /note="Trypanosoma cruzi trans-sialidase gene; mature sequence" FT CDS 3199..4090 FT /vntifkey="4" FT /label=fd\g3\fragment FT /note="fragment of phage fd gene 3" SQ Sequence 10878 BP; 2788 A; 2568 C; 2663 G; 2859 t; agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag 60 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt 120 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc 180 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta 240 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta 300 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg 360 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct 420 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg 480 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt 540 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct 600 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct 660 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact 720 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat 780 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt 840 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga 900 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct 960 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact 1020 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta 1080 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg 1140 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgagaaa 1200 agagaggctg aagcttacgt gctggcaccc ggatcgagcc gagttgagct gtttaagcgt 1260 aagaattcga cggtgccgtt tgaagacaag gccggcaaag tcaccgagcg ggttgtccac 1320 tcgttccgcc tccccgccct tgttaatgtg gacggggtga tggttgccat cgcggacgct 1380 cgctacgaca catccaatga caactccctc attgatacgg tggcgaagta cagcgtggac 1440 gatggggaga cgtgggagac ccaaattgcc atcaagaaca gccgtgtatc gtctgtttct 1500 cgtgtggtgg atcccaccgt gattgtgaag ggcaacaagc tttacgtcct ggttggaagc 1560 tactatagtt cgagaagcta ctggtcgtcg catggtgatg cgagagactg ggatattctg 1620 cttgccgttg gtgaggtcac gaagtccatt gcgggcggca agataactgc gagtatcaaa 1680 tgggggagcc ccgtgtcact gaagaagttt tttccggcag aaatggaagg catgcacaca 1740 aatcaatttc ttggcggcgc gggtgttgcc attgtggcgt ccaacgggaa tcttgtgtac 1800 cctgtgcagg ttacgaacaa aaggaagcaa gttttctcca agatcttcta ctcggaagat 1860 gatgggaaga cgtggaagtt tgggaagggt aggagcgatt ttggctgctc tgaacctgtg 1920 gcccttgagt gggaggggaa gctcatcata aacacccgag ttgactggaa acgccgtctg 1980 gtgtacgagt ccagtgacat ggggaatacg tgggtggagg ctgtcggcac gctctcgcgt 2040 gtgtggggcc cctcaccaaa atcggaccat cccggcagtc agagcagctt cactgccgtg 2100 accatcgaag gaatgcgtgt gatgctcttc acacacccgc tgaattttaa gggaaggtgg 2160 ctgcgcgacc gactgaacct ctggctgacg gataaccagc gcatttataa cgttgggcaa 2220 gtatccattg gtgatgaaaa ttccgcctac agctccgtcc tgtacaagga tgataagctg 2280 tactgtttgc atgagatcaa cacggacgag gtgtacagcc ttgtttttgc acgcctggtt 2340 ggcgagctac ggatcattaa atcagtgctg cgatcctgga agaattggga cagccacctg 2400 tccagcattt gcacccctgc tgatccagcc gcttcgtcgt cagagagtgg ttgtggtccc 2460 gctgtcacca cggttggtct tgttggcttt ttgtccggca atgcctccca aaacgtatgg 2520 gaggatgcgt accgctgcgt caacgcaagc acggcaaatg cggagagggt tcggaacggt 2580 ttgaagtttg cgggggttgg cggaggggcg ctttggccgg tgagccagca ggggcagaat 2640 cagcggtatc gttttgcaaa ccacgcgttc acgctggtgg cgtcggtgac gattcacgag 2700 gctccgaggg ccgcgagtcc tttgctgggt gcgagcctgg actcttctgg tggcaaaaaa 2760 ctcctggggc tctcgtacga cgagaagcac cagtggcagc caatatacgg atcaacgccg 2820 gtgacgccga cgggatcgtg ggagacgggt aagaggtacc acgtggttct tacggtggcg 2880 aataaaattg gctccgtgta cattgatgga gaacttctgg agggttcagg acagaccgtt 2940 gtgccagacg ggaggacgcc tgacatctcc cacttctacg ttggcgggta tggaaggagt 3000 gatatgccaa ccataagcca cgtgacggtg aataatgttc ttctttacaa ccgacagctg 3060 aataccgagg agatcaggac cttgttcttg agccaggacc tgattggcac ggaagcacac 3120 atggacagca gcagcgacac gaaggaattg ggtgcgccgg tgccgtatcc ggatccgctg 3180 gaaccgcgtg ccgcatagac tgttgaaagt tgtttagcaa aacctcatac agaaaattca 3240 tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggctgt 3300 ctgtggaatg ctacaggcgt tgtggtttgt actggtgacg aaactcagtg ttacggtaca 3360 tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt 3420 tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct 3480 attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa 3540 aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt 3600 cagaataata ggttccgaaa taggcagggt gcattaactg tttatacggg cactgttact 3660 caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg 3720 tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgag 3780 gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat 3840 gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggcgg ctctgagggt 3900 ggcggttctg agggtggcgg ctctgagggt ggcggttccg gtggcggctc cggttccggt 3960 gattttgatt atgaaaaaat ggcaaacgct aataaggggg ctatgaccga aaatgccgat 4020 gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt 4080 gctgctatcg ggccgcgaat taattcgcct tagacatgac tgttcctcag ttcaagttgg 4140 gcacttacga gaagaccggt cttgctagat tctaatcaag aggatgtcag aatgccattt 4200 gcctgagaga tgcaggcttc atttttgata cttttttatt tgtaacctat atagtatagg 4260 attttttttg tcattttgtt tcttctcgta cgagcttgct cctgatcagc ctatctcgca 4320 gctgatgaat atcttgtggt aggggtttgg gaaaatcatt cgagtttgat gtttttcttg 4380 gtatttccca ctcctcttca gagtacagaa gattaagtga gaagttcgtt tgtgcaagct 4440 tatcgataag ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc 4500 gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt 4560 aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga 4620 cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg 4680 cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc 4740 gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatcta 4800 tcgaatctaa atgtaagtta aaatctctaa ataattaaat aagtcccagt ttctccatac 4860 gaaccttaac agcattgcgg tgagcatcta gaccttcaac agcagccaga tccatcactg 4920 cttggccaat atgtttcagt ccctcaggag ttacgtcttg tgaagtgatg aacttctgga 4980 aggttgcagt gttaactccg ctgtattgac gggcatatcc gtacgttggc aaagtgtggt 5040 tggtaccgga ggagtaatct ccacaactct ctggagagta ggcaccaaca aacacagatc 5100 cagcgtgttg tacttgatca acataagaag aagcattctc gatttgcagg atcaagtgtt 5160 caggagcgta ctgattggac atttccaaag cctgctcgta ggttgcaacc gatagggttg 5220 tagagtgtgc aatacacttg cgtacaattt caacccttgg caactgcaca gcttggttgt 5280 gaacagcatc ttcaattctg gcaagctcct tgtctgtcat atcgacagcc aacagaatca 5340 cctgggaatc aataccatgt tcagcttgag acagaaggtc tgaggcaacg aaatctggat 5400 cagcgtattt atcagcaata actagaactt cagaaggccc agcaggcatg tcaatactac 5460 acagggctga tgtgtcattt tgaaccatca tcttggcagc agtaacgaac tggtttcctg 5520 gaccaaatat tttgtcacac ttaggaacag tttctgttcc gtaagccata gcagctactg 5580 cctgggcgcc tcctgctagc acgatacact tagcaccaac cttgtgggca acgtagatga 5640 cttctggggt aagggtacca tccttcttag gtggagatgc aaaaacaatt tctttgcaac 5700 cagcaacttt ggcaggaaca cccagcatca gggaagtgga aggcagaatt gcggttccac 5760 caggaatata gaggccaact ttctcaatag gtcttgcaaa acgagagcag actacaccag 5820 ggcaagtctc aacttgcaac gtctccgtta gttgagcttc atggaatttc ctgacgttat 5880 ctatagagag atcaatggct ctcttaacgt tatctggcaa ttgcataagt tcctctggga 5940 aaggagcttc taacacaggt gtcttcaaag cgactccatc aaacttggca gttagttcta 6000 aaagggcttt gtcaccattt tgacgaacat tgtcgacaat tggtttgact aattccataa 6060 tctgttccgt tttctggata ggacgacgaa gggcatcttc aatttcttgt gaggaggcct 6120 tagaaacgtc aattttgcac aattcaatac gaccttcaga agggacttct ttaggtttgg 6180 attcttcttt aggttgttcc ttggtgtatc ctggcttggc atctcctttc cttctagtga 6240 cctttaggga cttcatatcc aggtttctct ccacctcgtc caacgtcaca ccgtacttgg 6300 cacatctaac taatgcaaaa taaaataagt cagcacattc ccaggctata tcttccttgg 6360 atttagcttc tgcaagttca tcagcttcct ccctaatttt agcgttcaac aaaacttcgt 6420 cgtcaaataa ccgtttggta taagaacctt ctggagcatt gctcttacga tcccacaagg 6480 tggcttccat ggctctaaga ccctttgatt ggccaaaaca ggaagtgcgt tccaagtgac 6540 agaaaccaac acctgtttgt tcaaccacaa atttcaagca gtctccatca caatccaatt 6600 cgatacccag caacttttga gttgctccag atgtagcacc tttataccac aaaccgtgac 6660 gacgagattg gtagactcca gtttgtgtcc ttatagcctc cggaatagac tttttggacg 6720 agtacaccag gcccaacgag taattagaag agtcagccac caaagtagtg aatagaccat 6780 cggggcggtc agtagtcaaa gacgccaaca aaatttcact gacagggaac tttttgacat 6840 cttcagaaag ttcgtattca gtagtcaatt gccgagcatc aataatgggg attataccag 6900 aagcaacagt ggaagtcaca tctaccaact ttgcggtctc agaaaaagca taaacagttc 6960 tactaccgcc attagtgaaa cttttcaaat cgcccagtgg agaagaaaaa ggcacagcga 7020 tactagcatt agcgggcaag gatgcaactt tatcaaccag ggtcctatag ataaccctag 7080 cgcctgggat catcctttgg acaactcttt ctgccaaatc taggtccaaa atcacttcat 7140 tgataccatt attgtacaac ttgagcaagt tgtcgatcag ctcctcaaat tggtcctctg 7200 taacggatga ctcaacttgc acattaactt gaagctcagt cgattgagtg aacttgatca 7260 ggttgtgcag ctggtcagca gcatagggaa acacggcttt tcctaccaaa ctcaaggaat 7320 tatcaaactc tgcaacactt gcgtatgcag gtagcaaggg aaatgtcata cttgaagtcg 7380 gacagtgagt gtagtcttga gaaattctga agccgtattt ttattatcag tgagtcagtc 7440 atcaggagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg 7500 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc 7560 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg 7620 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac 7680 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagta tctatgattg 7740 gaagtatggg aatggtgata cccgcattct tcagtgtctt gaggtctcct atcagattat 7800 gcccaactaa agcaaccgga ggaggagatt tcatggtaaa tttctctgac ttttggtcat 7860 cagtagactc gaactgtgag actatctcgg ttatgacagc agaaatgtcc ttcttggaga 7920 cagtaaatga agtcccacca ataaagaaat ccttgttatc aggaacaaac ttcttgtttc 7980 gaactttttc ggtgccttga actataaaat gtagagtgga tatgtcgggt aggaatggag 8040 cgggcaaatg cttaccttct ggaccttcaa gaggtatgta gggtttgtag atactgatgc 8100 caacttcagt gacaacgttg ctatttcgtt caaaccattc cgaatccaga gaaatcaaag 8160 ttgtttgtct actattgatc caagccagtg cggtcttgaa actgacaata gtgtgctcgt 8220 gttttgaggt catctttgta tgaataaatc tagtctttga tctaaataat cttgacgagc 8280 caaggcgata aatacccaaa tctaaaactc ttttaaaacg ttaaaaggac aagtatgtct 8340 gcctgtatta aaccccaaat cagctcgtag tctgatcctc atcaacttga ggggcactat 8400 cttgttttag agaaatttgc ggagatgcga tatcgagaaa aaggtacgct gattttaaac 8460 gtgaaattta tctcaagatc tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg 8520 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 8580 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc 8640 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg 8700 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 8760 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 8820 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 8880 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 8940 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 9000 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 9060 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 9120 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc 9180 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 9240 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 9300 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 9360 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 9420 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 9480 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 9540 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 9600 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 9660 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 9720 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 9780 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 9840 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 9900 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 9960 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 10020 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 10080 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 10140 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 10200 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 10260 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 10320 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 10380 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 10440 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 10500 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 10560 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 10620 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 10680 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa 10740 aataggcgta tcacgaggcc ctttcgtctt caagaattaa ttctcatgtt tgacagctta 10800 tcatcgataa gctgactcat gttggtattg tgaaatagac gcagatcggg aacactgaaa 10860 aataacagtt attattcg 10878 //