LOCUS       pCAhIFNGSTmE 7729 bp dsDNA    Circular
DEFINITION  LMBP 4168.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     misc_difference 1218..1256
                     /label=E-tag
     misc_difference 69..137
                     /note="Human interferon gamma signal sequence (hIFNG)"
                     /label=hIFNG\signal\seq.
     intron          6754..7678
                     /label=intron\AG\promoter
     CDS             156..1217
                     /note="mutated mature human alpha2,6-sialyltransferase gene (hST6GalI)"
                     /label=hST6GalIm
     CDS             1266..2480
                     /note="fragment of phage fd gene 3"
                     /label=fd\g3f
     terminator      3019..3110
                     /label=ß-globin\polyA
     promoter        complement(3545..3627)
                     /label=lac\promoter
     rep_origin      3769..3844
                     /label=SV40\ori
     terminator      complement(3874..3949)
                     /label=SV40\polyA\late
     terminator      3874..3949
                     /label=SV40\polyA\early
     rep_origin      complement(4068..4468)
                     /label=pMB1\ori
     promoter        6002..6379
                     /label=hCMV\enhancer
     promoter        6385..7726
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
                     /label=AG\promoter
     CDS             complement(5006..5866)
                     /label=AMP
ORIGIN
        1 ttcctcgagg tcagcttgat acaagaacta ctgatttcaa cttctttggc ttaattctct 
       61 cggaaacgat gaaatataca agttatatct tggcttttca gctctgcatc gttttgggtt 
      121 ctcttggctg ttactgccag gacccagcgg ccgccatggg gtctgattcc cagtctgtat 
      181 cctcaagcag cacccaggac ccccacaggg gccgccagac cctcggcagt ctcagaggcc 
      241 tagccaaggc caaaccagag gcctccttcc aggtgtggaa caaggacagc tcttccaaaa 
      301 accttatccc taggctgcaa aagatctgga agaattacct aagcatgaac aagtacaaag 
      361 tgtcctacaa ggggccagga ccaggcatca agttcagtgc agaggccctg cgctgccacc 
      421 tccgggacca tgtgaatgta tccatggtag aggtcacaga ttttcccttc aatacctctg 
      481 aatgggaggg ttatctgccc aaggagagca ttaggaccaa ggctgggcct tggggcaggt 
      541 gtgctgttgt gtcgtcagcg ggatctctga agtcctccca actaggcaga gaaatcgatg 
      601 atcatgacgc agtcctgagg tttaatgggg cacccacagc caacttccaa caagatgtgg 
      661 gcacaaaaac taccattcgc ctgatgaact ctcagttggt taccacagag aagcgcttcc 
      721 tcaaagacag tttgtacaat gaaggaatcc taattgtatg ggacccatct gtataccact 
      781 cagatatccc aaagtggtac cagaatccgg attataattt ctttaacaac tacaagactt 
      841 atcgtaagct gcaccccaat cagccctttt acatcctcaa gccccagatg ccttgggagc 
      901 tatgggacat tcttcaagaa atctccccag aagagattca gccaaacccc ccatcctctg 
      961 ggatgcttgg tatcatcatc atgatgacgc tgtgtgacca ggtggatatt tatgagttcc 
     1021 tcccatccaa gcgcaagact gacgtgtgct actactacca gaagttcttc gatagtgcct 
     1081 gcacgatggg tgcctaccac ccgctgctct atgagaagaa tttggtgaag catctcaacc 
     1141 agggcacaga tgaggacatc tacctgcttg gaaaagccac actgcctggc ttccggacca 
     1201 ttcactgctg ccaattgggt gcgccggtgc cgtatccgga tccgctggaa ccgcgtgccg 
     1261 catagactgt tgaaagttgt ttagcaaaac ctcatacaga aaattcattt actaacgtct 
     1321 ggaaagacga caaaacttta gatcgttacg ctaactatga gggctgtctg tggaatgcta 
     1381 caggcgttgt ggtttgtact ggtgacgaaa ctcagtgtta cggtacatgg gttcctattg 
     1441 ggcttgctat ccctgaaaat gagggtggtg gctctgaggg tggcggttct gagggtggcg 
     1501 gttctgaggg tggcggtact aaacctcctg agtacggtga tacacctatt ccgggctata 
     1561 cttatatcaa ccctctcgac ggcacttatc cgcctggtac tgagcaaaac cccgctaatc 
     1621 ctaatccttc tcttgaggag tctcagcctc ttaatacttt catgtttcag aataataggt 
     1681 tccgaaatag gcagggtgca ttaactgttt atacgggcac tgttactcaa ggcactgacc 
     1741 ccgttaaaac ttattaccag tacactcctg tatcatcaaa agccatgtat gacgcttact 
     1801 ggaacggtaa attcagagac tgcgctttcc attctggctt taatgaggat ccattcgttt 
     1861 gtgaatatca aggccaatcg tctgacctgc ctcaacctcc tgtcaatgct ggcggcggct 
     1921 ctggtggtgg ttctggtggc ggctctgagg gtggcggctc tgagggtggc ggttctgagg 
     1981 gtggcggctc tgagggtggc ggttccggtg gcggctccgg ttccggtgat tttgattatg 
     2041 aaaaaatggc aaacgctaat aagggggcta tgaccgaaaa tgccgatgaa aacgcgctac 
     2101 agtctgacgc taaaggcaaa cttgattctg tcgctactga ttacggtgct gctatcgatg 
     2161 gtttcattgg tgacgtttcc ggccttgcta atggtaatgg tgctactggt gattttgctg 
     2221 gctctaattc ccaaatggct caagtcggtg acggtgataa ttcaccttta atgaataatt 
     2281 tccgtcaata tttaccttct ttgcctcagt cggttgaatg tcgcccttat gtctttggcg 
     2341 ctggtaaacc atatgaattt tctattgatt gtgacaaaat aaacttattc cgtggtgtct 
     2401 ttgcgtttct tttatatgtt gccaccttta tgtatgtatt ttcgacgttt gctaacatac 
     2461 tgcgtaataa ggagtcttaa taagaattgg gaattccccg cgcgcgatgc cctttcgtct 
     2521 tcgaaaagct gactaattat tcggtaactg acttgaatgt ccaacgcaaa gcaatacatg 
     2581 aactcatcca agtgatggct gaactgtcgc cagcagctaa aacagggaag cgaaaaagga 
     2641 gtcagatgct gtttcgaggt cgaagagcat cccagtaatg gttgtcctgc ctgcaatatt 
     2701 tgaattttaa atctaaatct atttattaat atttaacatt atttatatgg ggaatatatt 
     2761 tttagactca tcaatcaaat aagtatttat aatagcaact tttgtgtaat gaaaatgaat 
     2821 atctattaat atatgtatta tttataattc ctatatcctg tgactgtctc acttaatcct 
     2881 ttgttttctg actaattagg caaggctatg tgattacaag gctttatctc aggggccaac 
     2941 taggcagcca acctaagcaa gacctaggct tttgcaaaaa gctcaagctc caatgccctg 
     3001 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag 
     3061 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt 
     3121 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat 
     3181 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac 
     3241 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta 
     3301 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt 
     3361 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc 
     3421 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag 
     3481 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     3541 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctgggtgc ctaatgagtg 
     3601 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 
     3661 tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca 
     3721 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 
     3781 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 
     3841 gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg 
     3901 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 
     3961 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa 
     4021 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 
     4081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 
     4141 gcggtaatac ggttatccac agaatcaggg ataacgcagg aaagaacatg tgagcaaaag 
     4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 
     4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 
     4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 
     4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 
     4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 
     4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 
     4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 
     4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 
     4681 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 
     4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 
     4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 
     4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 
     4921 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 
     4981 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 
     5041 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 
     5101 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 
     5161 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 
     5221 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 
     5281 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 
     5341 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 
     5401 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 
     5461 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 
     5521 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 
     5581 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 
     5641 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 
     5701 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 
     5761 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 
     5821 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 
     5881 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
     5941 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt 
     6001 cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc 
     6061 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc 
     6121 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg 
     6181 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat 
     6241 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc 
     6301 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta 
     6361 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc 
     6421 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg 
     6481 atgggggcgg gggggggggg ggggcccccc ccaggcgggg cggggcgggg cgaggggcgg 
     6541 ggcggggcga ggcggaaagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt 
     6601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg 
     6661 ggagtcgttg cgcgctgcct tccccccgtg ccccgctccg ccgccgcctc gcgccgcccg 
     6721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct 
     6781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa 
     6841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc 
     6901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc 
     6961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg 
     7021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg 
     7081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac 
     7141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt 
     7201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg 
     7261 gggccgcctc gggccgggga gggctcgggg gaaggggcgc ggcggccccc ggagcgccgg 
     7321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc 
     7381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac 
     7441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga 
     7501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc 
     7561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt 
     7621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct 
     7681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaa 
//
