LOCUS       pCAGGS-mCASP-1          6308 bp    DNA     circular     26-OCT-2007
DEFINITION  LMBP 4175.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4175
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 26-OCT-2007
            
COMMENT     VNTNAME|pCAGGS-mCASP-1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-mCASP-1" 0 6308 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 21 22 62 29 30 32 33 43) 
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
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COMMENT       (CMolPar 0 0 0 0 4294967295 1 6308 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT            6307 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 6211 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 3 22 0 1496 0 2387 0 
COMMENT            0 "") #3=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 1480 0 0 "")) 
COMMENT       (CObList 
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COMMENT        #5=(CFSignal (CObList) "mCASP-1" 4 0 0 119 1327 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "119..1327") 
COMMENT        #6=(CFSignal (CObList) "3' UTR of mCASP-1" 21 0 0 1328 1438 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1328..1438") 
COMMENT        #7=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 1690 2046 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1690..2046") 
COMMENT        #8=(CFSignal (CObList) "lac promoter" 30 0 1 2124 2206 0 (CStringList) 
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COMMENT        #9=(CFSignal (CObList) "SV40 ori" 33 0 0 2348 2423 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2348..2423") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2454 2528 0 
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COMMENT        #11=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2454 2528 0 
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COMMENT        #12=(CFSignal (CObList) "pMB1 ori" 33 0 1 2643 3044 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "ampicillin" 4 0 1 3585 4445 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4581 4958 0 
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COMMENT        #15=(CFSignal (CObList) "AG promoter" 29 0 0 4964 6305 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "intron AG promoter" 15 0 0 5333 6257 0 
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COMMENT                          "DNA Plasmid 'pCAGGS-mCASP-1'" 1) 
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FEATURES             Location/Qualifiers
     misc_feature    42..118
                     /vntifkey="21"
                     /label=5'\UTR\of\mCASP-1
                     /note="5' UTR of the mouse caspase-1 gene"
     CDS             119..1327
                     /vntifkey="4"
                     /label=mCASP-1
                     /note="mouse caspase-1 coding sequence"
     misc_feature    1328..1438
                     /vntifkey="21"
                     /label=3'\UTR\of\mCASP-1
                     /note="3' UTR of the mouse caspase-1 gene"
     terminator      1598..1689
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     misc_feature    1690..2046
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     promoter        complement(2124..2206)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      2348..2423
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      2454..2528
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(2454..2528)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(2643..3044)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(3585..4445)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     promoter        4581..4958
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     promoter        4964..6305
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     intron          5333..6257
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="intron of the chicken ß-actin/rabbit ß-globin hybrid promoter"
BASE COUNT     1495 a      1573 c      1673 g      1567 t 
ORIGIN
        1 ttcctcgaag cttggccaaa acggccgagc tcgaattcgt ccggccgagc tcgaattcgt 
       61 caggccaaca caatatgctt tcagtttcag tagctctgcg gtgtagaaaa gaaacgccat 
      121 ggctgacaag atcctgaggg caaagaggaa gcaatttatc aactcagtga gtatagggac 
      181 aataaatgga ttgttggatg aacttttaga gaagagagtg ctgaatcagg aagaaatgga 
      241 taaaataaaa cttgcaaaca ttactgctat ggacaaggca cgggacctat gtgatcatgt 
      301 ctctaaaaaa gggccccagg caagccaaat ctttatcact tacatttgta atgaagactg 
      361 ctacctggca ggaattctgg agcttcaatc agctccatca gctgaaacat ttgttgctac 
      421 agaagattct aaaggaggac atccttcatc ctcagaaaca aaggaagaac agaacaaaga 
      481 agatggcaca tttccaggac tgactgggac cctcaagttt tgccctttag aaaaagccca 
      541 gaagttatgg aaagaaaatc cttcagagat ttatccaata atgaatacaa ccactcgtac 
      601 acgtcttgcc ctcattatct gcaacacaga gtttcaacat ctttctccga gggttggagc 
      661 tcaagttgac ctcagagaaa tgaagttgct gctggaggat ctggggtata ccgtgaaagt 
      721 gaaagaaaat ctcacagctc tggagatggt gaaagaggtg aaagaatttg ctgcctgccc 
      781 agagcacaag acttctgaca gtactttcct tgtattcatg tctcatggta tccaggaggg 
      841 aatatgtggg accacatact ctaatgaagt ttcagatatt ttaaaggttg acacaatctt 
      901 tcagatgatg aacactttga agtgcccaag cttgaaagac aagcccaagg tgatcattat 
      961 tcaggcatgc cgtggagaga aacaaggagt ggtgttgtta aaagattcag taagagactc 
     1021 tgaagaggat ttcttaacgg atgcaatttt tgaagatgat ggcattaaga aggcccatat 
     1081 agagaaagat tttattgctt tctgctcttc aacaccagat aatgtgtctt ggagacatcc 
     1141 tgtcaggggc tcacttttca ttgagtcact catcaaacac atgaaagaat atgcctggtc 
     1201 ttgtgacttg gaggacattt tcagaaaggt tcgattttca tttgaacaac cagaatttag 
     1261 gctacagatg cccactgctg atagggtgac cctgacaaaa cgtttctacc tcttcccggg 
     1321 acattaaacg aagaatccag ttcattctta tgtacctatg ctgagaatcg tgccaataag 
     1381 aagccaatac ttccttagat gatgcaataa atattaaaat aaaacaaaac agaaggctaa 
     1441 aaaaaaaaaa aaaaaagctc gcccggggat cctctaggcg gccgctaggc ctttttggcc 
     1501 aagctcgaat ttcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc 
     1561 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa 
     1621 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat 
     1681 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag 
     1741 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca 
     1801 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc 
     1861 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat 
     1921 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact 
     1981 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg 
     2041 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt 
     2101 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 
     2161 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 
     2221 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc 
     2281 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 
     2341 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 
     2401 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt 
     2461 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 
     2521 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 
     2581 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 
     2641 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 
     2701 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga 
     2761 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     2821 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     2881 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     2941 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     3001 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     3061 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     3121 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     3181 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     3241 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 
     3301 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     3361 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
     3421 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
     3481 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 
     3541 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 
     3601 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 
     3661 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 
     3721 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 
     3781 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 
     3841 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 
     3901 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 
     3961 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 
     4021 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 
     4081 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 
     4141 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 
     4201 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 
     4261 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 
     4321 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 
     4381 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 
     4441 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
     4501 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
     4561 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac 
     4621 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 
     4681 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 
     4741 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 
     4801 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 
     4861 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 
     4921 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt 
     4981 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt 
     5041 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc 
     5101 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag 
     5161 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa 
     5221 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc 
     5281 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg 
     5341 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc 
     5401 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag 
     5461 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct 
     5521 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg 
     5581 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa 
     5641 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg 
     5701 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc 
     5761 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt 
     5821 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg 
     5881 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat 
     5941 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat 
     6001 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca 
     6061 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc 
     6121 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg 
     6181 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct 
     6241 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg 
     6301 gcaaagaa 
//
