ID pCAGGS-mCASP-2 standard; circular DNA; ; 8408 BP. DE LMBP 4176. CC Accession number: LMBP 4176 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 2-MAR-2010 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_feature 88..170 FT /vntifkey="21" FT /label=5'\UTR\of\mCASP-2 FT CDS 171..1529 FT /vntifkey="4" FT /label=mCASP-2 FT misc_feature 1530..3548 FT /vntifkey="21" FT /label=3'\UTR\of\mCASP-2 FT terminator 3698..3789 FT /vntifkey="43" FT /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA FT misc_feature 3790..4146 FT /vntifkey="21" FT /label=3'\flanking\region\of\rabbit\ß-globin FT promoter complement(4224..4306) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin 4448..4523 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT terminator 4554..4628 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(4554..4628) FT /vntifkey="43" FT /label=SV40\polyA\late FT rep_origin complement(4743..5144) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(5685..6545) FT /vntifkey="4" FT /label=ampicillin\resistance FT promoter 6681..7058 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT promoter 7064..8405 FT /vntifkey="29" FT /label=AG\promoter FT intron 7433..8357 FT /vntifkey="15" FT /label=intron\AG\promoter SQ Sequence 8408 BP; 1808 A; 2124 C; 2188 G; 2288 t; ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg 60 ggatccccgg gaattcggca cgagggctgg gggagggatg cgggggctgg cgcggcccag 120 cgtttgtttg ttttgtgggt ggtgcgtgtg cagcccggga aaagctggaa atggcggcgc 180 cgagcgggag gtcgcagtcc tccctgcaca ggaaggggct gatggcggct gacaggagga 240 gcaggatttt ggcagtgtgt ggaatgcatc ctgaccacca ggaaacactg aaaaagaatc 300 gagtggtgct ggccaagcag ctgctgctga gcgagctgtt agaacacctc ctagagaagg 360 acattatcac tttggaaatg agggagctca tccaggccaa agggggcagt ttcagccaga 420 atgtggaact cctcaacctg ctgccaaaga gaggacccca ggcttttgat gccttctgtg 480 aagccctgcg ggagaccagg cagggtcact tggaagactt actgctcaca accctctcag 540 atattcagca cgtactccca ccgttgagct gtgactatga cacaagtctc cctttctcgg 600 tgtgtgagtc ctgccctcct cacaagcagc tccgcctatc cacagatgct acggaacact 660 ccttagataa tggtgatggt cctccctgtc ttctggtgaa gccatgcact cctgagtttt 720 accaggcaca ctaccagctg gcctataggt tgcaatctca gccccgtggc ttggcactgg 780 tgctgagcaa tgtgcacttc actggagaga aagacctgga attccgctct ggaggggatg 840 tggaccacac tactctagtc accctcttca agcttttggg ctacaatgtc catgtgctac 900 atgaccagac cgcacaggaa atgcaagaga aacttcagaa ttttgcacag ttacctgcac 960 accgggtcac agactcctgc gtagtggcac tcctctcaca tggtgtggaa ggtggcatct 1020 atggtgtaga tggcaaactg cttcagctcc aagaggtttt tcgacttttt gacaatgcta 1080 actgtccaag tctacagaac aagccaaaaa tgttcttcat ccaagcatgt cgtggagatg 1140 agacagatag aggtgtcgac cagcaagatg gaaagaacca cacacaatcc cctggatgtg 1200 aggagagtga tgctggcaaa gaggagttga tgaagatgag actgcctact cgctcagaca 1260 tgatatgtgg ctatgcttgc cttaaaggta atgctgccat gcggaacacc aaacggggtt 1320 cctggtacat tgaggccctc actcaggtgt tctctgaaag agcttgtgac atgcacgtgg 1380 ccgacatgct tgttaaggtg aatgccctta tcaaggagcg tgaaggctat gcccctggca 1440 cagaattcca ccgatgcaag gagatgtctg agtactgtag tactctgtgc cagcaactct 1500 acctgttccc aggctaccca cccacgtgat gccgcctgct attcctgctg ttggaggcca 1560 ctggaccact gggggcacaa tggagacttc tcttcagaat ggtttttgtt ctgtctaccc 1620 tctcagggat atgagattct cccaggcttg tttcctgtca gccatctctg tctttgggta 1680 tgaaacataa ggatggctcc tccggtgtcg tgttctctac ctatagagcc agctctgaat 1740 ggatgtgtta ccagaagcat tttagctaca gcctagaaaa tgacattgtg aacacagtat 1800 tattgtggga agagggcatt tggatttctc aatgtttgtg atatttttgt tcccaaggca 1860 tcttaggagt acttggatca tagctttttt tttttttcct aaatcagtta aggagtctca 1920 gagatcatct cctttttttt ccatatctac aacctcattt ttcccacagt ggagatttgg 1980 aagatgtccc aatttaatgt aggtgttttc atctgtcatg aagggacaga tgagatccta 2040 ctacttgcga agtttctatg cataccttta agttcaggcc ctaggtgaag gacagtccct 2100 cagcctttcc attggttcct ttgtgttcag tgcacccagc ctttgaacag agcctagggt 2160 ctgtatgcca tgacactgga agtcatagaa atttccctgg tcatgctttg tttgaactgt 2220 cactgaatga accttatcgg gcataactac atgaaaatgc agtgacagct gagtgtgctg 2280 tgtctcacac tatcacccgt catcaggatg tctctccttc cttactgtgg cttctgcatg 2340 cacttacact gtacttgacg gctggcctcc agggtctctc ttgctttgta ctggttcccc 2400 tctttacctt caccattcgc tgcttctgcc aagtctgtga agccgtcctt tgtaggatgt 2460 ttcttgccac ttacgctgta ctgtagttgc ttattctttc tgccttctgc ttcagcgtga 2520 ggcttctttg gttttctgtg gcagcgtctc ccttctcatt gtttctctgt gttttagtgg 2580 ggatagtacc atatgtgata taacctagaa gcacttgtct ctgctcttat gaaacttgct 2640 tattcttgaa aaccttctgc atttccattt tttcctctct tccaatttat tctccatgta 2700 acagagtagt ttggttttta aaatatctgg tgatgtcatt ctcttgctta gaacactagc 2760 ttcctgttac gcttcatcta aaatgcaaat tcttacaccc agcttacgag atctggctca 2820 taccttccct ttggatctca ttaaatggtg atttctcact atgctccagc ccctcttagg 2880 tcctcatctc cgtcttgcag gtgttctgaa ctctcctttg gctagtctct gatttttgag 2940 tctggcggag gcctcttgac catgctgccc atgctgtcta ctgtgcctcc ttatgagggc 3000 atcatgttgg tctctgttgt gcttactgca ggctgtaatg gcacttttgc ttgtttcact 3060 tgttccctct gaggctgaat gctccaagag agtgggaact gtgcttctta cttactgata 3120 tccagtaact ggcacttact aggtcttcat gaatgtttcc tgagtaaagg aaggagacca 3180 gcagctaacc ttagttagag cctacctttt gcagtttcta aattgctatt atagtgtaca 3240 gttcaattag tatatgggtt tttttttcca ggtgttttat ttttatccac tgttttgttg 3300 ttgttttttt atattttcta catatcacgt gtgagacaac ttctttcaca tctccatagt 3360 gcccagcaaa tttgaggcct atggtagttg aggtgctcaa ctaatgtttg ttgtatgaac 3420 caagtggttt gaagacttgc tgccaaattc tgccttttgg gtcagtatag gatgcataag 3480 tggtagaatc ttcacacttc ccactgccaa gattttgtat tgccatcagg tgccaaataa 3540 atgttgatct cgtgccgaat tcgatctctt tttttgggtt tggtggggta tcttcatcat 3600 cgaatagata gttatataca tcatccattg tagtggtatt aaacatccct gtagtgattc 3660 caaacgcgcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa 3720 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat 3780 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag 3840 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca 3900 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc 3960 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat 4020 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact 4080 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg 4140 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt 4200 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 4260 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 4320 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc 4380 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 4440 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 4500 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt 4560 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 4620 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 4680 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 4740 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 4800 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga 4860 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 4920 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 4980 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 5040 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 5100 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 5160 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 5220 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 5280 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 5340 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 5400 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 5460 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 5520 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 5580 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 5640 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 5700 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 5760 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 5820 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 5880 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 5940 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 6000 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 6060 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 6120 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 6180 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 6240 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 6300 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 6360 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 6420 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 6480 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 6540 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 6600 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 6660 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac 6720 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 6780 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 6840 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 6900 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 6960 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 7020 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt 7080 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt 7140 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc 7200 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag 7260 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa 7320 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc 7380 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg 7440 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc 7500 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag 7560 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct 7620 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg 7680 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa 7740 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg 7800 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc 7860 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt 7920 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg 7980 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat 8040 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat 8100 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca 8160 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc 8220 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg 8280 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct 8340 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg 8400 gcaaagaa 8408 //