LOCUS       pACTyEGFPTUHD1 6721 bp dsDNA  Circular
DEFINITION  LMBP 4178.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     rep_origin      complement(130..466)
                     /label=pMB1\ori
     CDS             complement(1010..1867)
                     /label=AMP
     CDS             complement(4956..5669)
                     /note="ORF: Frame #1   Start: atg (22)   Stop:  taa (735)"
                     /label=yEGFP3
     promoter        complement(5704..6591)
                     /note="oorspronkelijke positie 7230"
                     /label=CaACTIN\promoter
     terminator      complement(4482..4735)
                     /label=CaMAL2\terminator
     CDS             3470..4279
                     /label=CaURA3
     CDS             2174..2890
                     /label=CaHIS1\fragment
ORIGIN
        1 gatctctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 
       61 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
      121 cagatcgatc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 
      181 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 
      241 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 
      301 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 
      361 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 
      421 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 
      481 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 
      541 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 
      601 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 
      661 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 
      721 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 
      781 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 
      841 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 
      901 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 
      961 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 
     1021 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 
     1081 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 
     1141 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 
     1201 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 
     1261 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 
     1321 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 
     1381 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 
     1441 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 
     1501 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 
     1561 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 
     1621 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 
     1681 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 
     1741 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 
     1801 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 
     1861 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 
     1921 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 
     1981 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 
     2041 ataggcgtat cacgaggccc tttcgtcttc aagaattctc atgtttgaca gcttatcatc 
     2101 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 
     2161 gctattacgc caggctgata tcccagtttt cgttggagaa ggtaattgtg acttgggtat 
     2221 aactgggtta gaccaaatca aagaagctga acaattcgac aacatcgagg acttgttgga 
     2281 tttgaaattt ggttcatgca aattgcagat ccaagttcca gcagatggcg agtacgaaaa 
     2341 gccagaacag cttgttggaa agaaaattgt gtcttcattt acaaaattga gtaccgacta 
     2401 tttcaaacaa ttgtcagaca aacctactaa tatcagatat gtcggtggtt ccgttgaggc 
     2461 ttcttgtgcc ttgggtgttg ctgatgctat tgtcgatttg gttgaaagtg gtgaaactat 
     2521 gaaagcagct gggttaaagg ccatagaaac catattggag acttcggctc atttgatttc 
     2581 gtcaaaaaaa agtaaattcc cagaaatggt caatataatc gtccaaagac ttcaaggtgt 
     2641 tttagctgcc caggaatatg tcttgtgtaa ctacaatgcc ccaaaatcca tccaagcaaa 
     2701 atgtttaacc attactccag gcagaagggc cgctactgtc tctactttgg acaaacacag 
     2761 cgacgacgaa gaggactggg ttgccatctc atccatggtt aatagaaagg aaatcggtaa 
     2821 tgtaatggac gaattgaaga aagctggtgc aaccgatata ttggtactcg agatatcaaa 
     2881 ttgtagagtt taaaagaagt gatagtttct cataaatgac tctagaggta ttccccgggt 
     2941 accgagctcg aattctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc 
     3001 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattccca ctaataggaa 
     3061 ttgatttgga tggtataaac ggaaacaaaa aaaagagctg gtactacttt ctttaaaatt 
     3121 attttattat ttgattttat ttaatagtat atattatatt ttgaacgtag attattttgt 
     3181 tgaaagttgc tgtagtgcca ttgattcgta acactaattc tgtattagtc attcctcttg 
     3241 tttgatagta tccaaaaaaa cggctatttt tttgcaatct tatttcctgc atattataca 
     3301 gataacataa tgaaagaaaa aatctttttt tttgttcttc aatgatgatt tcaaccattc 
     3361 ttttaaacat tgatcaattc ctgagcaaca accccataca cactggttta tataccgccc 
     3421 cttttacagt tgaagaaaga aatagaaata gaaatagcaa acaaaagata tgacagtcaa 
     3481 cactaagacc tatagtgaga gagcagaaac tcatgcctca ccagtagcac agcgattatt 
     3541 tcgattaatg gaactgaaga aaaccaattt atgtgcatca attgacgttg ataccactaa 
     3601 ggaattcctt gaattaattg ataaattagg tccttatgta tgcttaatca agactcatat 
     3661 tgatataatc aatgattttt cctatgaatc cactattgaa ccattattag aactttcacg 
     3721 taaacatcaa tttatgattt ttgaagatag aaaatttgct gatattggta ataccgtaaa 
     3781 gaaacaatat attggtggag tttataaaat tagtagttgg gcagatatta ccaatgctca 
     3841 tggtgtcact gggaatggag tggttgaagg attaaaacag ggagctaaag aaaccaccac 
     3901 caaccaagag ccaagagggt tattgatgtt agctgaatta tcatcagtgg gatcattagc 
     3961 atatggagaa tattctcaaa aaactgttga aattgctaaa tccgataagg aatttgttat 
     4021 tggatttatt gcccaacgtg atatgggtgg ccaagaagaa ggatttgatt ggcttattat 
     4081 gacacctgga gttggattag atgataaagg tgatggatta ggacaacaat atagaactgt 
     4141 tgatgaagtt gttagcactg gaactgatat tatcattgtt ggtagaggat tgtttggtaa 
     4201 aggaagagat ccagatattg aaggtaaaag gtatagaaat gctggttgga atgcttattt 
     4261 gaaaaagact ggccaattat aaatgtgaag ggggagattt tcactttatt agatttgtat 
     4321 atatgtagaa taaataaata aataagttaa ataaataatt aaataagggt ggtaattatt 
     4381 actatttaca atcaaaggtg gtccttctag ctgtaatccg ggcagcgcaa cggaacattc 
     4441 atcagtgtaa aaatggaatc aataaagccc tgcggggatc catacgcttt gcaggtggtg 
     4501 ttgatccaaa ttccgtgctt ggtcctctac tcaatatacc agaactttga aaatgtcgag 
     4561 cagacattca cgttgatttt cccacggtgg gatatcatcg ccacactcat ttccatatac 
     4621 ttgttcacct acatctacgc cgagggtaaa tcaaactact ttaaaggtgt cattttgata 
     4681 ttgatctatg ttgttgttct ctctgggttc tggtttaacg atattatcga tagcatctag 
     4741 tgtatattta atcgaattcc tctgagctca atttttgttt tcttaatatc ccatgaataa 
     4801 tcacttctac tcaacgtgcc acttacagga tgttgatctg attctaggac gactgtgtcg 
     4861 taaaaaacat aacctatctt atttttttaa actaagtttc tataaaccat acaaaactaa 
     4921 ttnctataga gctgccaatt tttaaaactg cattatttgt acaattcatc cataccatgg 
     4981 gtaataccag cagcagtaac aaattctaac aagaccatgt ggtctctctt ttcgtttgga 
     5041 tctttggata aggcagattg agtggataag taatggttgt ctggtaacaa gactggacca 
     5101 tcaccaattg gagtattttg ttgataatgg tcagctaatt gaacagaacc atcttcaatg 
     5161 ttgtgtctaa ttttgaagtt aactttgata ccattctttt gtttgtcagc catgatgtaa 
     5221 acattgtgag agttatagtt gtattccaat ttgtgaccta aaatgttacc atcttcttta 
     5281 aaatcaatac cttttaattc gattctatta actaaggtat caccttcaaa cttgacttca 
     5341 gctctggtct tgtagttacc gtcatctttg aaaaaaatag ttctttcttg aacataacct 
     5401 tctggcatgg cagacttgaa aaagtcatgt tgtttcatat gatctgggta tctagcaaaa 
     5461 cattgaacac cataaccgaa agtagtgact aaggttggcc atggaactgg caatttacca 
     5521 gtagtacaaa taaattttaa ggtcaattta ccgtaagtag catcaccttc accttcaccg 
     5581 gagacagaaa atttgtgacc attaacatca ccatctaatt caaccaaaat tgggacaaca 
     5641 ccagtgaata attcttcacc tttagacatt ttaataaagc tctagggggg atccctcgaa 
     5701 attcttatat ttttttaata ttaatatcga gataatgttt ctaagaaaga aagaaaacca 
     5761 ggagtgaaaa ttagaaaagg aaaggaaagg aaaaaaagaa aaatctgaaa atatataaaa 
     5821 aaaaattgtt tcgttggcaa taaatcctgg tgagaacagc gaccgaaagc aaataagaac 
     5881 aaaatataag tgtattacgt tgaacaacta attaacgtgt gtgtatggat ctttttttct 
     5941 tttttctctt taaccgacta taaacaacaa acatttttgg gcagtgcaca cacactactt 
     6001 aatatacaca gcataaatta cacgattaga aaacaaatta gcttattaaa ataacctaat 
     6061 caaaccgaat attttatggt attacgagta aactatataa tataaatagc acacacccac 
     6121 aacaacaaca aaggaaaact aaaaattttt tctttttgaa agggccgttt tctttattat 
     6181 tctctagttt tgacgctgaa atattccttg tactgttgca aatttgtatt tggtctgcct 
     6241 tatcttcttt tctacacttt ttacttatga gctctataat tcacagataa gtttcaggtt 
     6301 tgccagatat acttgcttca ttggttacta aactctctgt aaacaattga cttgcttatg 
     6361 taagaggcta cttttctagt tttgcagtca ttcatttaaa aacaattgat cctgaaggct 
     6421 ctaaagacat agtaggttta ccttgagtgt atatttccgt ggcatgttta aaggcccctt 
     6481 ggccatagga tattggtggc ggcacagtaa gcattaaaat tgttgtttcg cggaattaca 
     6541 acatttcttc ttttacataa taatgtcact cctttttggt tggcatagcc gagatctttt 
     6601 tttaacacag actttaagct gttttttgcc acctgagaac agtttctatt ctggtccaaa 
     6661 agaaatatta gactaagttt attataaacg aggctggtga tcttaatagc tctatcggac 
     6721 g 
//
