LOCUS       pACTyEGFPTULD1 7256 bp dsDNA  Circular
DEFINITION  LMBP 4181.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     rep_origin      complement(130..466)
                     /label=pMB1\ori
     CDS             complement(1010..1867)
                     /label=AMP
     CDS             complement(5491..6204)
                     /note="ORF: Frame #1   Start: atg (22)   Stop:  taa (735)"
                     /label=yEGFP3
     promoter        complement(6239..7126)
                     /note="oorspronkelijke positie 7230"
                     /label=CaACTIN\promoter
     terminator      complement(5017..5270)
                     /label=CaMAL2\terminator
     CDS             4005..4814
                     /label=CaURA3
     CDS             complement(2424..3489)
                     /label=CaLEU2\fragment
ORIGIN
        1 gatctctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 
       61 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
      121 cagatcgatc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 
      181 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 
      241 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 
      301 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 
      361 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 
      421 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 
      481 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 
      541 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 
      601 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 
      661 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 
      721 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 
      781 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 
      841 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 
      901 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 
      961 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 
     1021 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 
     1081 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 
     1141 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 
     1201 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 
     1261 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 
     1321 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 
     1381 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 
     1441 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 
     1501 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 
     1561 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 
     1621 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 
     1681 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 
     1741 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 
     1801 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 
     1861 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 
     1921 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 
     1981 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 
     2041 ataggcgtat cacgaggccc tttcgtcttc aagaattctc atgtttgaca gcttatcatc 
     2101 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 
     2161 gctattacgc caggctataa cattgacgag cagtagtgag gagaagagtt tttctctccg 
     2221 aatgaagagc caaatcatat acgagtgcca agtcaatccg aatcaagcga ggccaccatt 
     2281 acatctacca ggcaaaaaaa ttttgcaaat aagcaacctt tattgagtga agattcggta 
     2341 actcagttgt gtgcatattt agagaaatct gtagactttg gagtatttga aatttaaatg 
     2401 ctaactactg tatatactgt ttatgtattt tgttttaaaa ttttagtgac tgtttcggct 
     2461 attgcgtcac cgacttctgt agtagagcta gtacctctta aatctgctgt tctgataccg 
     2521 ctatccaaaa cctgtttgac agcttcttcc aatgcttcag tttccttaac acaatccaag 
     2581 gctaatctca acatggaagc ggcagacaag attgtagcaa ttggattcac tttgttggct 
     2641 ggtaaatctg gagcagaccc atggcaaggc tcataaagac caaaagcagt gttggtatct 
     2701 ggcaatgaag ccaatgaagc agatggcaat aaacccaaag atcctgggat aacggatgcc 
     2761 tcatctgaga tgatatcacc aaacatgtta gatgtaatga tgataccgct caatttggtt 
     2821 gggttttgga tcaaaatcat tgcagccgaa tcaatcaatt gatgctgcac agacaaagct 
     2881 ggaaattctt cagaaatcac tttatcaacg gtctttctcc acaatctcga cgatgccaaa 
     2941 acattagctt tgtccaaaga ccatattggc aatggtggat tgtgctgcaa agccataaat 
     3001 gctgccatac gagtgatacg agtaacttcg tcaacagtat actttggtct ataccaagct 
     3061 gtttggtggt cttcactttc ttcttgttct tgacgttcac caaaatatat accaccaact 
     3121 aattcacgaa caataattag attggttcct ttgaccactt cagcttttaa tggagacaat 
     3181 tctaataacg aatcactggc aaagttacat ggtctaatgt tggcataaag gttcaattct 
     3241 ttacggattt tcaacaatcc ttgttcagga cgaacagtgc cagtacccca tttgggtcca 
     3301 ccaacagcac ccaacaaaac tgcatcggat gatttagcac tttcaagagc atcatctgga 
     3361 agcgggacgc cagtggcatc aatggcagca ccaccaatca agtggtgttt gaaatcaaat 
     3421 tggatttttt ggtagggagt gggagcttca attgctttca agactttggt tggttcattg 
     3481 acaatttcgc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg 
     3541 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcccactaat aggaattgat 
     3601 ttggatggta taaacggaaa caaaaaaaag agctggtact actttcttta aaattatttt 
     3661 attatttgat tttatttaat agtatatatt atattttgaa cgtagattat tttgttgaaa 
     3721 gttgctgtag tgccattgat tcgtaacact aattctgtat tagtcattcc tcttgtttga 
     3781 tagtatccaa aaaaacggct atttttttgc aatcttattt cctgcatatt atacagataa 
     3841 cataatgaaa gaaaaaatct ttttttttgt tcttcaatga tgatttcaac cattctttta 
     3901 aacattgatc aattcctgag caacaacccc atacacactg gtttatatac cgcccctttt 
     3961 acagttgaag aaagaaatag aaatagaaat agcaaacaaa agatatgaca gtcaacacta 
     4021 agacctatag tgagagagca gaaactcatg cctcaccagt agcacagcga ttatttcgat 
     4081 taatggaact gaagaaaacc aatttatgtg catcaattga cgttgatacc actaaggaat 
     4141 tccttgaatt aattgataaa ttaggtcctt atgtatgctt aatcaagact catattgata 
     4201 taatcaatga tttttcctat gaatccacta ttgaaccatt attagaactt tcacgtaaac 
     4261 atcaatttat gatttttgaa gatagaaaat ttgctgatat tggtaatacc gtaaagaaac 
     4321 aatatattgg tggagtttat aaaattagta gttgggcaga tattaccaat gctcatggtg 
     4381 tcactgggaa tggagtggtt gaaggattaa aacagggagc taaagaaacc accaccaacc 
     4441 aagagccaag agggttattg atgttagctg aattatcatc agtgggatca ttagcatatg 
     4501 gagaatattc tcaaaaaact gttgaaattg ctaaatccga taaggaattt gttattggat 
     4561 ttattgccca acgtgatatg ggtggccaag aagaaggatt tgattggctt attatgacac 
     4621 ctggagttgg attagatgat aaaggtgatg gattaggaca acaatataga actgttgatg 
     4681 aagttgttag cactggaact gatattatca ttgttggtag aggattgttt ggtaaaggaa 
     4741 gagatccaga tattgaaggt aaaaggtata gaaatgctgg ttggaatgct tatttgaaaa 
     4801 agactggcca attataaatg tgaaggggga gattttcact ttattagatt tgtatatatg 
     4861 tagaataaat aaataaataa gttaaataaa taattaaata agggtggtaa ttattactat 
     4921 ttacaatcaa aggtggtcct tctagctgta atccgggcag cgcaacggaa cattcatcag 
     4981 tgtaaaaatg gaatcaataa agccctgcgg ggatccatac gctttgcagg tggtgttgat 
     5041 ccaaattccg tgcttggtcc tctactcaat ataccagaac tttgaaaatg tcgagcagac 
     5101 attcacgttg attttcccac ggtgggatat catcgccaca ctcatttcca tatacttgtt 
     5161 cacctacatc tacgccgagg gtaaatcaaa ctactttaaa ggtgtcattt tgatattgat 
     5221 ctatgttgtt gttctctctg ggttctggtt taacgatatt atcgatagca tctagtgtat 
     5281 atttaatcga attcctctga gctcaatttt tgttttctta atatcccatg aataatcact 
     5341 tctactcaac gtgccactta caggatgttg atctgattct aggacgactg tgtcgtaaaa 
     5401 aacataacct atcttatttt tttaaactaa gtttctataa accatacaaa actaattnct 
     5461 atagagctgc caatttttaa aactgcatta tttgtacaat tcatccatac catgggtaat 
     5521 accagcagca gtaacaaatt ctaacaagac catgtggtct ctcttttcgt ttggatcttt 
     5581 ggataaggca gattgagtgg ataagtaatg gttgtctggt aacaagactg gaccatcacc 
     5641 aattggagta ttttgttgat aatggtcagc taattgaaca gaaccatctt caatgttgtg 
     5701 tctaattttg aagttaactt tgataccatt cttttgtttg tcagccatga tgtaaacatt 
     5761 gtgagagtta tagttgtatt ccaatttgtg acctaaaatg ttaccatctt ctttaaaatc 
     5821 aatacctttt aattcgattc tattaactaa ggtatcacct tcaaacttga cttcagctct 
     5881 ggtcttgtag ttaccgtcat ctttgaaaaa aatagttctt tcttgaacat aaccttctgg 
     5941 catggcagac ttgaaaaagt catgttgttt catatgatct gggtatctag caaaacattg 
     6001 aacaccataa ccgaaagtag tgactaaggt tggccatgga actggcaatt taccagtagt 
     6061 acaaataaat tttaaggtca atttaccgta agtagcatca ccttcacctt caccggagac 
     6121 agaaaatttg tgaccattaa catcaccatc taattcaacc aaaattggga caacaccagt 
     6181 gaataattct tcacctttag acattttaat aaagctctag gggggatccc tcgaaattct 
     6241 tatatttttt taatattaat atcgagataa tgtttctaag aaagaaagaa aaccaggagt 
     6301 gaaaattaga aaaggaaagg aaaggaaaaa aagaaaaatc tgaaaatata taaaaaaaaa 
     6361 ttgtttcgtt ggcaataaat cctggtgaga acagcgaccg aaagcaaata agaacaaaat 
     6421 ataagtgtat tacgttgaac aactaattaa cgtgtgtgta tggatctttt tttctttttt 
     6481 ctctttaacc gactataaac aacaaacatt tttgggcagt gcacacacac tacttaatat 
     6541 acacagcata aattacacga ttagaaaaca aattagctta ttaaaataac ctaatcaaac 
     6601 cgaatatttt atggtattac gagtaaacta tataatataa atagcacaca cccacaacaa 
     6661 caacaaagga aaactaaaaa ttttttcttt ttgaaagggc cgttttcttt attattctct 
     6721 agttttgacg ctgaaatatt ccttgtactg ttgcaaattt gtatttggtc tgccttatct 
     6781 tcttttctac actttttact tatgagctct ataattcaca gataagtttc aggtttgcca 
     6841 gatatacttg cttcattggt tactaaactc tctgtaaaca attgacttgc ttatgtaaga 
     6901 ggctactttt ctagttttgc agtcattcat ttaaaaacaa ttgatcctga aggctctaaa 
     6961 gacatagtag gtttaccttg agtgtatatt tccgtggcat gtttaaaggc cccttggcca 
     7021 taggatattg gtggcggcac agtaagcatt aaaattgttg tttcgcggaa ttacaacatt 
     7081 tcttctttta cataataatg tcactccttt ttggttggca tagccgagat ctttttttaa 
     7141 cacagacttt aagctgtttt ttgccacctg agaacagttt ctattctggt ccaaaagaaa 
     7201 tattagacta agtttattat aaacgaggct ggtgatctta atagctctat cggacg 
//
