LOCUS       pACTyEGFPTH 5499 bp dsDNA    Circular
DEFINITION  LMBP 4190.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     CDS             complement(4431..5291)
                     /label=AMP
     CDS             complement(1659..2372)
                     /note="ORF: Frame #1   Start: atg (22)   Stop:  taa (735)"
                     /label=yEGFP3
     CDS             237..1130
                     /note="ORF: Frame #3   Start: atg   Stop:  taa"
                     /label=CaHIS1
     promoter        complement(2407..3294)
                     /note="oorspronkelijke positie 7230"
                     /label=CaACTIN\promoter
     terminator      complement(1185..1438)
                     /label=CaMAL2\terminator
     rep_origin      complement(3554..3890)
                     /label=pMB1\ori
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgcgcc attcgccatt caggctgcgc aactgttggg 
       61 aagggcgatc ggtgcgggcc tcttcgctat tacgccagga ctctagagga tcccccgagc 
      121 ggttgtgaga attgactcat tttgattatt acgcaaccat tgaaattttt tatttttttt 
      181 tggtgaagat ttttcccaca caacttcttc ttttacttaa ccaccaacta ccgataatgg 
      241 atttagtcaa tcatttacca gaccgtttgt tatttgctgt tcctaaaaag ggcagattat 
      301 acgaaaaatg ctgtaactta ttgagtggtg ccgatataca gtttagaaga tctaatagat 
      361 tagatatagc actttctaca aacttgccaa ttgcattaat cttcttgcct gcagctgata 
      421 tcccagtttt cgttggagaa ggtaattgtg acttgggtat aactgggtta gaccaaatca 
      481 aagaagctga acaattcgac aacatcgagg acttgttgga tttgaaattt ggttcatgca 
      541 aattgcagat ccaagttcca gcagatggcg agtacgaaaa gccagaacag cttgttggaa 
      601 agaaaattgt gtcttcattt acaaaattga gtaccgacta tttcaaacaa ttgtcagaca 
      661 aacctactaa tatcagatat gtcggtggtt ccgttgaggc ttcttgtgcc ttgggtgttg 
      721 ctgatgctat tgtcgatttg gttgaaagtg gtgaaactat gaaagcagct gggttaaagg 
      781 ccatagaaac catattggag acttcggctc atttgatttc gtcaaaaaaa agtaaattcc 
      841 cagaaatggt caatataatc gtccaaagac ttcaaggtgt tttagctgcc caggaatatg 
      901 tcttgtgtaa ctacaatgcc ccaaaatcca tccaagcaaa atgtttaacc attactccag 
      961 gcagaagggc cgctactgtc tctactttgg acaaacacag cgacgacgaa gaggactggg 
     1021 ttgccatctc atccatggtt aatagaaagg aaatcggtaa tgtaatggac gaattgaaga 
     1081 aagctggtgc aaccgatata ttggtactcg agatatcaaa ttgtagagtt taaaagaagt 
     1141 gatagtttct cataaatgac tctagaggta ttccccgggg atccatacgc tttgcaggtg 
     1201 gtgttgatcc aaattccgtg cttggtcctc tactcaatat accagaactt tgaaaatgtc 
     1261 gagcagacat tcacgttgat tttcccacgg tgggatatca tcgccacact catttccata 
     1321 tacttgttca cctacatcta cgccgagggt aaatcaaact actttaaagg tgtcattttg 
     1381 atattgatct atgttgttgt tctctctggg ttctggttta acgatattat cgatagcatc 
     1441 tagtgtatat ttaatcgaat tcctctgagc tcaatttttg ttttcttaat atcccatgaa 
     1501 taatcacttc tactcaacgt gccacttaca ggatgttgat ctgattctag gacgactgtg 
     1561 tcgtaaaaaa cataacctat cttatttttt taaactaagt ttctataaac catacaaaac 
     1621 taattnctat agagctgcca atttttaaaa ctgcattatt tgtacaattc atccatacca 
     1681 tgggtaatac cagcagcagt aacaaattct aacaagacca tgtggtctct cttttcgttt 
     1741 ggatctttgg ataaggcaga ttgagtggat aagtaatggt tgtctggtaa caagactgga 
     1801 ccatcaccaa ttggagtatt ttgttgataa tggtcagcta attgaacaga accatcttca 
     1861 atgttgtgtc taattttgaa gttaactttg ataccattct tttgtttgtc agccatgatg 
     1921 taaacattgt gagagttata gttgtattcc aatttgtgac ctaaaatgtt accatcttct 
     1981 ttaaaatcaa taccttttaa ttcgattcta ttaactaagg tatcaccttc aaacttgact 
     2041 tcagctctgg tcttgtagtt accgtcatct ttgaaaaaaa tagttctttc ttgaacataa 
     2101 ccttctggca tggcagactt gaaaaagtca tgttgtttca tatgatctgg gtatctagca 
     2161 aaacattgaa caccataacc gaaagtagtg actaaggttg gccatggaac tggcaattta 
     2221 ccagtagtac aaataaattt taaggtcaat ttaccgtaag tagcatcacc ttcaccttca 
     2281 ccggagacag aaaatttgtg accattaaca tcaccatcta attcaaccaa aattgggaca 
     2341 acaccagtga ataattcttc acctttagac attttaataa agctctaggg gggatccctc 
     2401 gaaattctta tattttttta atattaatat cgagataatg tttctaagaa agaaagaaaa 
     2461 ccaggagtga aaattagaaa aggaaaggaa aggaaaaaaa gaaaaatctg aaaatatata 
     2521 aaaaaaaatt gtttcgttgg caataaatcc tggtgagaac agcgaccgaa agcaaataag 
     2581 aacaaaatat aagtgtatta cgttgaacaa ctaattaacg tgtgtgtatg gatctttttt 
     2641 tcttttttct ctttaaccga ctataaacaa caaacatttt tgggcagtgc acacacacta 
     2701 cttaatatac acagcataaa ttacacgatt agaaaacaaa ttagcttatt aaaataacct 
     2761 aatcaaaccg aatattttat ggtattacga gtaaactata taatataaat agcacacacc 
     2821 cacaacaaca acaaaggaaa actaaaaatt ttttcttttt gaaagggccg ttttctttat 
     2881 tattctctag ttttgacgct gaaatattcc ttgtactgtt gcaaatttgt atttggtctg 
     2941 ccttatcttc ttttctacac tttttactta tgagctctat aattcacaga taagtttcag 
     3001 gtttgccaga tatacttgct tcattggtta ctaaactctc tgtaaacaat tgacttgctt 
     3061 atgtaagagg ctacttttct agttttgcag tcattcattt aaaaacaatt gatcctgaag 
     3121 gctctaaaga catagtaggt ttaccttgag tgtatatttc cgtggcatgt ttaaaggccc 
     3181 cttggccata ggatattggt ggcggcacag taagcattaa aattgttgtt tcgcggaatt 
     3241 acaacatttc ttcttttaca taataatgtc actccttttt ggttggcata gccgagatct 
     3301 ttttttaaca cagactttaa gctgtttttt gccacctgag aacagtttct attctggtcc 
     3361 aaaagaaata ttagactaag tttattataa acgaggctgg tgatcttaat agctctatcg 
     3421 gacggatctc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 
     3481 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     3541 gtatcagatc gatctcactc aaaggcggta atacggttat ccacagaatc aggggataac 
     3601 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 
     3661 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 
     3721 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 
     3781 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 
     3841 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 
     3901 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 
     3961 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 
     4021 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 
     4081 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 
     4141 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 
     4201 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 
     4261 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 
     4321 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 
     4381 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 
     4441 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 
     4501 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 
     4561 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 
     4621 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 
     4681 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 
     4741 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 
     4801 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 
     4861 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 
     4921 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 
     4981 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 
     5041 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 
     5101 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 
     5161 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 
     5221 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 
     5281 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 
     5341 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 
     5401 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 
     5461 aaaaataggc gtatcacgag gccctttcgt cttcaagaa 
//
