LOCUS       pSCGAL1mBAX-L  5846 bp dsDNA    Circular
DEFINITION  LMBP 4200.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     rep_origin      complement(1842..2178)
                     /label=pMB1\ori
     CDS             complement(2719..3579)
                     /note="Antibiotic resistance gene"
                     /label=AMP
     CDS             4723..5814
                     /note="ORF: Frame #3   Start: atg (2664)   Stop:  taa (3755)"
                     /label=LEU2
     terminator      50..320
                     /note="Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)"
                     /label=CYC1\terminator
     CDS             complement(634..1209)
                     /note="mouse Bax sequence encoding the Bax-alpha protein (mBAX)"
                     /label=mBAX
     promoter        complement(1317..1700)
                     /note="S. cerevisiae galactokinase promoter (GAL1)"
                     /label=GAL1\promoter
     terminator      complement(323..427)
                     /note="S. cerevisiae 2 micron plasmid (2µ) FLP terminator"
                     /label=FLP\terminator
ORIGIN
        1 gctggcgcct gctgcgcgac ggcgagctgc tcaccaccca ggatccgtcc cccttttcct 
       61 ttgtcgatat catgtaatta gttatgtcac gcttacattc acgccctccc cccacatccg 
      121 ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta 
      181 tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca 
      241 gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct 
      301 cgaaggcttt aatttgcaag ctgcacatac agctcactgt tcacgtcgca cctatatctg 
      361 cgtgttgcct gtatatatat atacatgaga agaacggcat agtgcgtgtt tatgcttaaa 
      421 tgcgtactta tatgcgtcta tttatgtagg atgaaaggta gtctagtacc tcctgtgata 
      481 ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc actacccttt agctgttcta 
      541 tatgctgcca ctcctcaatt ggattagtct catccttcaa tgctatcatt tcctttgata 
      601 ttggatcata agcttgcatg cctgcaggtc tcagcccatc ttcttccaga tggtgagcga 
      661 ggcggtgagg actccagcca caaagatggt cactgtctgc catgtggggg tcccgaagta 
      721 ggagaggagg ccttcccagc caccctggtc ttggatccag acaagcagcc gctcacggag 
      781 gaagtccagt gtccagccca tgatggttct gatcagctcg ggcactttag tgcacagggc 
      841 cttgagcacc agtttgctag caaagtagaa gagggcaacc acgcggcccc agttgaagtt 
      901 gccatcagca aacatgtcag ctgccacccg gaagaagacc tctcgggggg agtccgtgtc 
      961 cacgtcagca atcatcctct gcagctccat attgctatcc agttcatctc caattcgccg 
     1021 gagacactcg ctcagcttct tggtggacgc atcctggggc ggctgctcca aggtcagctc 
     1081 aggtgtctcc ccagccatcc tccctgctcg atcctggatg aaaccctgta gcaaaaaggc 
     1141 ccctgtcttc atgatctgtt cagagctggt gggcccgccg ctcccaagct gctccccgga 
     1201 cccgtccatg actctagagg atcacaatca cttcttctga atgagattta gtcacgatcg 
     1261 ttttttctcc ttgacgttaa agtatagagg tatattaaca attttttgtt gatactttta 
     1321 ttacatttga ataagaagta atacaaaccg aaaatgttga aagtattagt taaagtggtt 
     1381 atgcagtttt tgcatttata tatctgttaa tagatcaaaa atcatcgctt cgctgattaa 
     1441 ttaccccaga aataaggcta aaaaactaat cgcattatca tcctatggtt gttaatttga 
     1501 ttcgttcatt tgaaggtttg tggggccagg ttactgccaa tttttcctct tcataaccat 
     1561 aaaagctagt attgtagaat ctttattgtt cggagcagtg cggcgcgagg cacatctgcg 
     1621 tttcaggaac gcgaccggtg aagacgagga cgcacggagg agagtcttcc ttcggagggc 
     1681 tgtcacccgc tcggcggctt ctaatccgtc cggatctctg cattaatgaa tcggccaacg 
     1741 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 
     1801 gcgctcggtc gttcggctgc ggcgagcggt atcagatcga tctcactcaa aggcggtaat 
     1861 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 
     1921 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 
     1981 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 
     2041 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 
     2101 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 
     2161 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 
     2221 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 
     2281 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 
     2341 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 
     2401 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 
     2461 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 
     2521 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 
     2581 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 
     2641 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 
     2701 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 
     2761 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 
     2821 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 
     2881 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 
     2941 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 
     3001 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 
     3061 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 
     3121 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 
     3181 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 
     3241 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 
     3301 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 
     3361 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 
     3421 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 
     3481 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 
     3541 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 
     3601 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 
     3661 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga 
     3721 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct 
     3781 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 
     3841 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 
     3901 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca 
     3961 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca 
     4021 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt 
     4081 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt 
     4141 ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta cccgtgactg 
     4201 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 
     4261 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 
     4321 gttttcaccg tcatcaccga aacgcgcgag gcagaactgt gggaatactc aggtatcgta 
     4381 agatgcaaga gttcgaatct cttagcaacc attatttttt tcctcaacat aacgagaaca 
     4441 cacaggggcg ctatcacaca gaatcaaatt cgatgactgg aaatttttca actgaaaaat 
     4501 tgggagaaaa aggaaaggtg agagcgccgg aaccggcttt tcatatagaa tagagaagcg 
     4561 ttcatgacta aatgcttgca tcacaatact tgaagttgac aatattattt aaggacctat 
     4621 tgttttttcc aataggtggt tagcaatcgt cttactttct aacttttctt accttttaca 
     4681 tttcagcaat atatatatat atttcaagga tataccattc taatgtttgt tcctaagaag 
     4741 atcgtcgttt tgccaggtga ccacgttggt caagaaatca cagccgaagc cattaaggtt 
     4801 cttaaagcta tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt 
     4861 ggtggtgctg ctatcgatgc tacaggtgtc ccacttccag atgaggcact ggaagcctcc 
     4921 aagaaggttg atgccgtttt gttaggtgct gtgggtggtc caaaatgggg taccggtagt 
     4981 gttagacctg aacaaggttt actaaaaatc cgtaaagaac ttcaattgta cgccaactta 
     5041 agaccatgta actttgcatc cgactctctt ttagacttat ctccaatcaa gccacaattt 
     5101 gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg gaggtattta ctttggtaag 
     5161 agaaaggaag acgatggtga tggtgtcgct tgggatagtg aacaatacac cgttccagaa 
     5221 gtgcaaagaa tcacaagaat ggccgctttc atggccctac aacatgagcc accattgcct 
     5281 atttggacct tggataaagc taatgttttg gcctcttcaa gattatggag aaaaactgtg 
     5341 gaggaaacca tcaagaacga attccctaca ttgaaggttc aacatcaatt gattgattct 
     5401 gccgccatga tcctagttaa gaacccaacc cacctaaatg gtattataat caccagcaac 
     5461 atgtttggtg atatcatctc cgatgaagcc ttacgttatc caggttcctt gggtttgttg 
     5521 ccatctgcgt ccttggcctc tttgccagac aagaacaccg catttggttt gtacgaacca 
     5581 tgccacggtt ctgctcccgg atttgccaag aataaggttg accctatcgc cactatcttg 
     5641 tctgctgcaa tgatgttgaa attgtcattg aacttgcctg aagaaggtaa ggccattgaa 
     5701 gatgcagtta aaaaggtttt ggatgcaggt atcagaactg gtgatttagg tggttccaac 
     5761 agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct tgcttaaaaa 
     5821 gattctcttt tttatgatat ttgtac 
//
