ID pCaMET15 standard; circular DNA; ; 7258 BP. DE LMBP 4218. CC Accession number: LMBP 4218 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 29-AUG-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT rep_origin complement(264..666) FT /vntifkey="33" FT /label=pMB1\ori FT /note="Escherichia coli plasmid pMB1 origin" FT CDS complement(1207..2067) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT CDS 3692..4504 FT /vntifkey="4" FT /label=CaURA3 FT /note="C. albicans uracil 3 coding sequence" FT CDS complement(5116..6438) FT /vntifkey="4" FT /label=CaMET15 FT /note="Candida albicans O-acetylhomoserine O-acetylserine sulfhydrylase coding sequence" FT terminator 6988..7258 FT /vntifkey="43" FT /label=CYC1\terminator FT /note="Saccharomyces cerevisiae iso-1-cytochrome C terminator" FT promoter complement(6439..6979) FT /vntifkey="29" FT /label=CaMET15\promoter FT /note="Candida albicans O-acetylhomoserine O-acetylserine sulfhydrylase promoter" FT misc_feature complement(4804..5115) FT /vntifkey="21" FT /label=3'\UTR\of\CaMET15,\incl.\polyA FT /note="3' UTR of Candida albicans O-acetylhomoserine O-acetylserine sulfhydrylase gene, incl. polyA" FT promoter complement(12..28) FT /vntifkey="30" FT /label=SP6\promoter FT /note="Phage SP6 promoter" FT promoter complement(89..172) FT /vntifkey="30" FT /label=lac\promoter FT /note="Escherichia coli lac operon promoter" FT rep_origin complement(2506..2961) FT /vntifkey="33" FT /label=f1\ori FT /note="Phage f1 origin" FT promoter 3125..3141 FT /vntifkey="30" FT /label=T7\promoter FT /note="Phage T7 gene 10 promoter (T7g10)" SQ Sequence 7258 BP; 2143 A; 1477 C; 1583 G; 2055 t; cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc 60 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 120 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 180 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 240 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 300 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 360 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 420 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 480 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 540 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 600 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 660 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 720 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 780 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 840 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 900 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 960 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 1020 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 1080 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 1140 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 1200 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 1260 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 1320 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 1380 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 1440 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 1500 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 1560 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 1620 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 1680 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 1740 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 1800 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 1860 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 1920 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 1980 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 2040 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 2100 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 2160 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 2220 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 2280 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 2340 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 2400 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 2460 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgaaatt gtaaacgtta 2520 atattttgtt aaaattcgcg ttaaatattt gttaaatcag ctcatttttt aaccaatagg 2580 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg 2640 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa 2700 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca agttttttgc 2760 ggtcgaggtg ccgtaaagct ctaaatcgga accctaaagg gagcccccga tttagagctt 2820 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg 2880 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta 2940 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg 3000 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 3060 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga 3120 attgtaatac gactcactat agggcgaatt ggttttccaa tgatgagcac ttttaaagtt 3180 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc 3240 atacactatt ctcagaatga cttggttgag tactaatagg aattgatttg gatggtataa 3300 acggaaacaa aaaaaagagc tggtactact ttctttaaaa ttattttatt atttgatttt 3360 atttaatagt atatattata ttttgaacgt agattatttt gttgaaagtt gctgtagtgc 3420 cattgattcg taacactaat tctgtattag tcattcctct tgtttgatag tatccaaaaa 3480 aacggctatt tttttgcaat cttatttcct gcatattata cagataacat aatgaaagaa 3540 aaaatctttt tttttgttct tcaatgatga tttcaaccat tcttttaaac attgatcaat 3600 tcctgagcaa caaccccata cacactggtt tatataccgc cccttttaca gttgaagaaa 3660 gaaatagaaa tagaaatagc aaacaaaaga tatgacagtc aacactaaga cctatagtga 3720 gagagcagaa actcatgcct caccagtagc acagcgatta tttcgattaa tggaactgaa 3780 gaaaaccaat ttatgtgcat caattgacgt tgataccact aaggaattcc ttgaattaat 3840 tgataaatta ggtccttatg tatgcttaat caagactcat attgatataa tcaatgattt 3900 ttcctatgaa tccactattg aaccattatt agaactttca cgtaaacatc aatttatgat 3960 ttttgaagat agaaaatttg ctgatattgg taataccgta aagaaacaat atattggtgg 4020 agtttataaa attagtagtt gggcagatat taccaatgct catggtgtca ctgggaatgg 4080 agtggttgaa ggattaaaac agggagctaa agaaaccacc accaaccaag agccaagagg 4140 gttattgatg ttagctgaat tatcatcagt gggatcatta gcatatggag aatattctca 4200 aaaaactgtt gaaattgcta aatccgataa ggaatttgtt attggattta ttgcccaacg 4260 tgatatgggt ggccaagaag aaggatttga ttggcttatt atgacacctg gagttggatt 4320 agatgataaa ggtgatggat taggacaaca atatagaact gttgatgaag ttgttagcac 4380 tggaactgat attatcattg ttggtagagg attgtttggt aaaggaagag atccagatat 4440 tgaaggtaaa aggtatagaa atgctggttg gaatgcttat ttgaaaaaga ctggccaatt 4500 ataaatgtga agggggagat tttcacttta ttagatttgt atatatgtag aataaataaa 4560 taaataagtt aaataaataa ttaaataagg gtggtaatta ttactattta caatcaaagg 4620 tggtccttct agctgtaatc cgggcagcgc aacggaacat tcatcagtgt aaaaatggaa 4680 tcaataaagc cctgcgcagc gcgcagggtc agcctgaata cgcgtttaat gaccagcaca 4740 gtcgtgatgg caaggtcaga atagcccaag tcggccgagg ggcctgtaca gtgagggaag 4800 atcgatctca tctttgattt gcttagctgt gactaccgct acctcttttt ttgaaatgat 4860 aggttactgt gtttttgcaa aatctttggc tagagtcttt ttcattgtca aggtcatatc 4920 tatgttggtt ttcgtcttca caatatgtat actatactgc ataatcatgg ttatgaaaaa 4980 ctttttaacc gatttaatca cccaatttca aattggaacc ctagagttcc cctccatcaa 5040 atggattact tggggtgttt taacattaat gtgtctaatt attgttgtga aacgttatct 5100 tatataccaa tctatttagt tgttataaac cttcttgaat gcttgttcaa agtcgttaat 5160 aatatcatca atatattcag taccagtaga aactctgatt aagcccttgg taacaccaga 5220 agccaacttt tcttcatcag acaattgttg atgtgtagta aaccatggag caataaccaa 5280 agtcttagag tcacccacat tagccaagtt tgaagcaatc tccaaattgt caaccaactt 5340 tggtgaggct tcattgaatg ggtcgctgga ggtgttggtg atgtccttga cagtaaatgc 5400 taaagcacca ccaaagtact tagcgtcatt gttcaagtat tttttactca attcgtggga 5460 ttcgtgagat ggcaatccca aataggacac agactcaaca tttgggttct tttctaacca 5520 ttgggccaat ttcaaagcat tttcagattg tctttcaact ctcaaagaca aagtttccaa 5580 accttgcaac aacaaaaaac ttccaaatgg attcaaagct ggacccaagt ctctcaacaa 5640 ttcaattctc aagtgaccaa tgtatgcggc cttacctaaa gcatcattca agaccaaccc 5700 gtggtaacct tctgatggtt tagagaattg tgggtatttt tctgggtact cggtccatgg 5760 gaagttaccg gaatcaacaa taacaccagc aattgtagta ccgtgaccac caatccattt 5820 agtagcagaa tgaacaacaa tatcagcacc gtgggcaatt gggttaacta aaaatccacc 5880 agcaccaaaa gtgttgtcga caacaacagg aataccgtgt tcatgggcca atttggtgat 5940 tttttcaaag tctggaacat tatatttagg gtttccaatg gtttcaatat aaatagcttt 6000 tgtcttgtcg tcaatcaatt tagcaaaatc ttcagcggcg tcaccattaa cgaatttggt 6060 ttcaatgccc aaacgtttga aagcaacttt gaattgatta taagtaccac catacaagta 6120 ggatgtactg ataatgttat caccagcatg agccaaccca gcaatagcca agaattgagc 6180 tgattgacca gaagaagtgg ccaatgcacc aataccacct tccaaggcag caattctttg 6240 ttcaaacaca tcgtttgttg gattcataat tctggagtaa atgtatcctg gggtttctaa 6300 accaaataat tgagcaccgt gtttagagtc attgaaaaca taggaggtgg ttgcataaat 6360 tggtggggct cttggttggt gtggtttttc aactggttgc ccagcatgta attgaagtgt 6420 atcaaagtga gaaggcattg tagtttagta atggggaaaa caaaaaagga agaattaaaa 6480 caaaaaaaaa aagtggctct aaagaatatt tatatttgtg aaaaacgtcg attcgtatag 6540 gctatttata ttatctcaaa ataatttcat catggaccga gaactgtgga tgcaaagcct 6600 ataaactttt aaccaccaca ggaggaaaag tcaataacgc ggagtgaatt gagggacaac 6660 aaaaaaaaac tgttaccatt tcacgtgaac tgtcaaagta cccaagaaat taactattaa 6720 aattcgtaat ttagaaaaca atagtgattc cgaaaccgct ctactgtttt agacttttca 6780 ttgaggcact ggttatagtg gtggctttac agcattacca tattgtagac tttacagttt 6840 atcaccaagt ttctacccaa gaccacttca aggtgtttta tttttcattt tttttggcaa 6900 atttttttct acaaaatatt ctctgcttca acatacattt gttatgttga gaaacaggaa 6960 aattgaaaac aaaagaattg atccgtcccc cttttccttt gtcgatatca tgtaattagt 7020 tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt 7080 tagacaacct gaagtctagg tccctattta tttttttata gttatgttag tattaagaac 7140 gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat 7200 tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcaag 7258 //