LOCUS       pAS3                    9534 bp    DNA     circular      8-JAN-2008
DEFINITION  LMBP 4253.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4253
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 8-JAN-2008
            
COMMENT     VNTNAME|pAS3|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAS3" 0 9534 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "AatII" "BglI" "BglII" "BstXI" "EcoRV" "HindIII" "NcoI" 
COMMENT         "NdeI" "SspI" "XhoI" "AccIII" "AvaII" "Csp45I" "Bst98I" "MunI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 9534 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 3874 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 2 7130 0 8738 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BglII" "agatct" 2 0 2 2195 0 4069 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 2 2555 0 3625 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 3 2225 0 3651 0 5487 0 0 
COMMENT            "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 5 2974 0 3986 0 5171 0 
COMMENT            6598 0 7366 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 7125 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "NdeI" "catatg" 3 0 2 4821 0 7119 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SspI" "aatatt" 4 0 8 880 0 3167 0 3364 0 4128 
COMMENT            0 4367 0 4566 0 6564 0 9422 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 6840 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "AccIII" "tccgga" 2 0 3 1749 0 3981 0 4161 0 0 
COMMENT             "") 
COMMENT        #11=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 4 391 0 1915 0 8756 0 8978 
COMMENT             0 0 "") 
COMMENT        #12=(CRSite (CStringList) "Csp45I" "ttcgaa" 3 0 1 3027 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "Bst98I" "cttaag" 2 0 3 4177 0 5400 0 5873 0 0 
COMMENT             "") 
COMMENT        #14=(CRSite (CStringList) "MunI" "caattg" 2 0 6 1294 0 2024 0 2586 0 
COMMENT             3514 0 4100 0 4979 0 0 "")) 
COMMENT       (CObList 
COMMENT        #15=(CFSignal (CObList) "2mu ori" 33 0 1 2 982 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2..982)") 
COMMENT        #16=(CFSignal (CObList) "ADE2" 4 0 0 3477 5192 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3477..5192") 
COMMENT        #17=(CFSignal (CObList) "pMB1 ori" 33 0 1 7601 8003 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(7601..8003)") 
COMMENT        #18=(CFSignal (CObList) "ADH1 terminator" 43 0 0 7172 7273 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "7172..7273") 
COMMENT        #19=(CFSignal (CObList) "HA epitope" 22 0 0 7078 7104 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7078..7104") 
COMMENT        #20=(CFSignal (CObList) "GALbd" 4 0 0 6622 7062 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "6622..7062") 
COMMENT        #21=(CFSignal (CObList) "ADH1 promoter" 29 0 0 6196 6599 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "6196..6599") 
COMMENT        #22=(CFSignal (CObList) "ampicillin" 4 0 1 8544 9404 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(8544..9404)") 
COMMENT        #23=(CFSignal (CObList) "FLP terminator" 43 0 1 1060 1164 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #24=(CFSignal (CObList) "lac promoter" 30 0 1 7426 7509 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #25=(CFSignal (CObList) "LMBP 4253" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #26=(CFSignal (CObList) "Last modified: 8-JAN-2008" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT             (CObjectList 
COMMENT              #28=(CRefLinePar 
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COMMENT                    (CObjectList 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pAS3'" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4253" 
COMMENT                          1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/08/08 04:38" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 9534 bp" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #38=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #40=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
COMMENT              #43=(CRefLinePar 
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COMMENT                   0 0) 
COMMENT              #44=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #46=(CLinePar (CParagraph 0 (15 #25# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 4253") 
COMMENT                     #47=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #48=(CLinePar (CParagraph 0 (15 #26# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 8-JAN-2008"))) 
COMMENT                   "Annotations") 
COMMENT              #49=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #50=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #51=(CFolderPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3477  End: 5192" 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3477..5192" 1))) "ADE2") 
COMMENT                            #55=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6622 (3 #20# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6622  End: 7062" 1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   6622..7062" 1))) "GALbd") 
COMMENT                            #59=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 8544 (3 #22# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 8544  End: 9404 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(8544..9404)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (3 total)") 
COMMENT                     #63=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7078  End: 7104" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   7078..7104" 1))) "HA epitope"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #68=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
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COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6196  End: 6599" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   6196..6599" 1))) "ADH1 promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
COMMENT                     #73=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #74=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7426 (3 #24# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7426  End: 7509 (Complementary)" 
COMMENT                                        1))) "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #76=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #77=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2     End: 982  (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2..982)" 1))) "2mu ori") 
COMMENT                            #81=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7601 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7601  End: 8003 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(7601..8003)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
COMMENT                     #85=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #86=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1060 (3 #23# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1060  End: 1164 (Complementary)" 
COMMENT                                        1))) "FLP terminator") 
COMMENT                            #88=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7172 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7172  End: 7273" 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   7172..7273" 1))) "ADH1 terminator"))) 
COMMENT                          "Terminator (2 total)"))) "Feature Map") 
COMMENT              #92=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #93=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #94=(CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 3874 " 1))))) "AatII: 1 site") 5 1 
COMMENT                          "GACGTC" "CTGCAG") 
COMMENT                     #96=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #97=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 7125 " 1))))) "NcoI: 1 site") 1 5 
COMMENT                          "CCATGG" "GGTACC") 
COMMENT                     #99=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #100=(CGroupPar 
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COMMENT                                   (CObjectList 
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COMMENT                                          (CParagraph 0 (1 #9# 1) 1 2 2 0 191) 
COMMENT                                          " N1: 6840 " 1))))) "XhoI: 1 site") 1 
COMMENT                          5 "CTCGAG" "GAGCTC") 
COMMENT                     #102=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
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COMMENT                                    (CObjectList 
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COMMENT                                           (CParagraph 0 (1 #12# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 3027 " 1))))) "Csp45I: 1 site") 
COMMENT                           2 4 "TTCGAA" "AAGCTT") 
COMMENT                     #105=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #106=(CGroupPar 
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COMMENT                                    (CObjectList 
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COMMENT                                           (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 7130 " 1) 
COMMENT                                     #108=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 8738 " 1))))) "BglI: 2 sites") 
COMMENT                           7 4 "GCCNNNNNGGC" "CGGNNNNNCCG") 
COMMENT                     #109=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #110=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #111=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 2195 " 1) 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #2# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 4069 " 1))))) "BglII: 2 sites") 
COMMENT                           1 5 "AGATCT" "TCTAGA") 
COMMENT                     #113=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
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COMMENT                                    (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
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COMMENT                                           (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 2555 " 1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 3625 " 1))))) "BstXI: 2 sites") 
COMMENT                           8 4 "CCANNNNNNTGG" "GGTNNNNNNACC") 
COMMENT                     #117=(CRSFolderPar 
COMMENT                           (CFolderPar 
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COMMENT                             (CObjectList 
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COMMENT                                    (CObjectList 
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COMMENT                                           " N1: 4821 " 1) 
COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #7# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 7119 " 1))))) "NdeI: 2 sites") 
COMMENT                           2 4 "CATATG" "GTATAC") 
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COMMENT                                    (CObjectList 
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COMMENT                                           " N1: 2225 " 1) 
COMMENT                                     #124=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 3651 " 1) 
COMMENT                                     #125=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #4# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 5487 " 1))))) "EcoRV: 3 sites") 
COMMENT                           3 3 "GATATC" "CTATAG") 
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COMMENT                           (CFolderPar 
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COMMENT                                    (CObjectList 
COMMENT                                     #128=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #10# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 1749 " 1) 
COMMENT                                     #129=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #10# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 3981 " 1) 
COMMENT                                     #130=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #10# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 4161 " 1))))) "AccIII: 3 sites") 
COMMENT                           1 5 "TCCGGA" "AGGCCT") 
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COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178) 
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COMMENT                                    (CParagraph 0 (10 #13# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #133=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #13# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 4177 " 1) 
COMMENT                                     #134=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #13# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 5400 " 1) 
COMMENT                                     #135=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #13# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 5873 " 1))))) "Bst98I: 3 sites") 
COMMENT                           1 5 "CTTAAG" "GAATTC") 
COMMENT                     #136=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 4 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
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FEATURES             Location/Qualifiers
     rep_origin      complement(2..982)
                     /vntifkey="33"
                     /label=2mu\ori
     CDS             3477..5192
                     /vntifkey="4"
                     /label=ADE2
     rep_origin      complement(7601..8003)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      7172..7273
                     /vntifkey="43"
                     /label=ADH1\terminator
     misc_marker     7078..7104
                     /vntifkey="22"
                     /label=HA\epitope
     CDS             6622..7062
                     /vntifkey="4"
                     /label=GALbd
     promoter        6196..6599
                     /vntifkey="29"
                     /label=ADH1\promoter
     CDS             complement(8544..9404)
                     /vntifkey="4"
                     /label=ampicillin
     terminator      complement(1060..1164)
                     /vntifkey="43"
                     /label=FLP\terminator
     promoter        complement(7426..7509)
                     /vntifkey="30"
                     /label=lac\promoter
BASE COUNT     2820 a      1970 c      1965 g      2777 t         2 others
ORIGIN
        1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 
       61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 
      121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 
      181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 
      241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 
      301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 
      361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 
      421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 
      481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 
      541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 
      601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 
      661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 
      721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 
      781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 
      841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 
      901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 
      961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 
     1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 
     1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 
     1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 
     1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 
     1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 
     1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 
     1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 
     1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 
     1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 
     1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 
     1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 
     1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 
     1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 
     1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 
     1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 
     1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 
     1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 
     2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 
     2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 
     2161 gcattgatnc cacggtgtta tagtatcagc aatagatctt tttactcaaa tttacagttt 
     2221 tgatatcttg gcgtttttcc tcacatttat tacaaaagaa agtgggcagg atttgccctt 
     2281 cacaaaatct ctgtccagga gaagaacgta tttgaccaga attgctcaat cttcttcatt 
     2341 gagacagttt ttacaactga gtacacgccc taatataagg attctgggag gtaatgggca 
     2401 gggtactgga aattccaatt atcccgaatt tacaaacgca tcctcagtca tctcacccaa 
     2461 gctaagggct tctcctctac tagaaagaag ggcgtcaaaa atctgctatg ctattacgaa 
     2521 attactctat aggttagaat gtcatcccat aggtatggcc cttttaaaga agtatctcca 
     2581 caatcaattg cgagaagcct atctagaatc aaaacgacac tttatttcca aaaagggaga 
     2641 ttcgaccaac acttcctcta ccattgcatc atcatctttc gctggcgcat ctgttcctct 
     2701 atcttcaaac gaatcaggaa tgctaaacgg cttgaagcaa attaacgaac aacaagaatc 
     2761 tacattagaa accactcaaa aggaagacta gtaacgccgt atcgtgatta acgtattaca 
     2821 taagttacag gattcatgct tatgggttag ctatttcgcc caatgtgtcc atctgacatt 
     2881 actattttgc attttaattt aattagaact tgactagcgc actaccagta tatcatctca 
     2941 tttccgtaaa taccaaatgt attatatatt gaaagctttt gaccaggtta ttataaaaga 
     3001 aacttcatgc tcgaaaaaga tcatttcgaa aagttgccta gtttcatgaa attttaaagc 
     3061 agtttatata aattttacct tttgatgcgg aattgacttt ttcttgaata atacataact 
     3121 tttcttaaaa gaatcaaaga cagataaaat ttaagagata ttaaatatta gtgagaagcc 
     3181 gagaattttg taacaccaac ataacactga catctttaac aacttttaat tatgatacat 
     3241 ttcttacgtc atgattgatt attacagcta tgctgacaaa tgactcttgt tgcatggcta 
     3301 cgaaccgggt aatactaagt gattgactct tgctgacctt ttattaagaa ctaaatggac 
     3361 aatattatgg agcatttcat gtataaattg gtgcgtaaaa tcgttggatc tctcttctaa 
     3421 gtacatccta ctataacaat caagaaaaac aagaaaatcg gacaaaacaa tcaagtatgg 
     3481 attctagaac agttggtata ttaggagggg gacaattggg acgtatgatt gttgaggcag 
     3541 caaacaggct caacattaag acggtaatac tagatgctga aaattctcct gccaaacaaa 
     3601 taagcaactc caatgaccac gttaatggct ccttttccaa tcctcttgat atcgaaaaac 
     3661 tagctgaaaa atgtgatgtg ctaacgattg agattgagca tgttgatgtt cctacactaa 
     3721 agaatcttca agtaaaacat cccaaattaa aaatttaccc ttctccagaa acaatcagat 
     3781 tgatacaaga caaatatatt caaaaagagc atttaatcaa aaatggtata gcagttaccc 
     3841 aaagtgttcc tgtggaacaa gccagtgaga cgtccctatt gaatgttgga agagatttgg 
     3901 gttttccatt cgtcttgaag tcgaggactt tggcatacga tggaagaggt aacttcgttg 
     3961 taaagaataa ggaaatgatt ccggaagctt tggaagtact gaaggatcgt cctttgtacg 
     4021 ccgaaaaatg ggcaccattt actaaagaat tagcagtcat gattgtgaga tctgttaacg 
     4081 gtttagtgtt ttcttaccca attgtagaga ctatccacaa ggacaatatt tgtgacttat 
     4141 gttatgcgcc tgctagagtt ccggactccg ttcaacttaa ggcgaagttg ttggcagaaa 
     4201 atgcaatcaa atcttttccc ggttgtggta tatttggtgt ggaaatgttc tatttagaaa 
     4261 caggggaatt gcttattaac gaaattgccc caaggcctca caactctgga cattatacca 
     4321 ttgatgcttg cgtcacttct caatttgaag ctcatttgag atcaatattg gatttgccaa 
     4381 tgccaaagaa tttcacatct ttctccacca ttacaacgaa cgccattatg ctaaatgttc 
     4441 ttggagacaa acatacaaaa gataaagagc tagaaacttg cgaaagagca ttggcgactc 
     4501 caggttcctc agtgtactta tatggaaaag agtctagacc taacagaaaa gtaggtcaca 
     4561 taaatattat tgcctccagt atggcggaat gtgaacaaag gctgaactac attacaggta 
     4621 gaactgatat tccaatcaaa atctctgtcg ctcaaaagtt ggacttggaa gcaatggtca 
     4681 aaccattggt tggaatcatc atgggatcag actctgactt gccggtaatg tctgccgcat 
     4741 gtgcggtttt aaaagatttt ggcgttccat ttgaagtgac aatagtctct gctcatagaa 
     4801 ctccacatag gatgtcagca tatgctattt ccgcaagcaa gcgtggaatt aaaacaatta 
     4861 tcgctggagc tggtggggct gctcacttgc caggtatggt ggctgcaatg acaccacttc 
     4921 ctgtcatcgg tgtgcccgta aaaggttctt gtctagatgg agtagattct ttacattcaa 
     4981 ttgtgcaaat gcctagaggt gttccagtag ctaccgtcgc tattaataat agtacgaacg 
     5041 ctgcgctgtt ggctgtcaga ctgcttggcg cttatgattc aagttataca acgaaaatgg 
     5101 aacagttttt attaaagcaa gaagaagaag ttcttgtcaa agcacaaaag ttagaaactg 
     5161 tcggttacga agcttatcta gaaaacaagt aatatataag tttattgata tacttgtaca 
     5221 gcaaataatt ataaaatgat atacctattt tttaggcttt gttatgatta catcaaatgt 
     5281 ggacttcata catagaaatc aacgcttaca ggtgtccttt tttaagaatt tcatacataa 
     5341 gatcacttat tatacataca tacatatcca gtaacaagaa gcaaggaata attacctgct 
     5401 taagtctgcg attaaaaaaa taacgtttcg atacagttca tataaggcgg ctcaatgcag 
     5461 aaccgaggat agcgctacgt caggatatct ttgtagttcc caaatataaa tgcgacaata 
     5521 tagtttcttt ctttcatatc aataatatcc ttttctccac tgaaatcacg aatcaaacct 
     5581 ggagcaaaaa ctaaagccaa attataaagc gtcattcgat tccagtgact gtaccgtgta 
     5641 accttttcta tatgttcact cagtactctt aacaccctat aatgttccct tggaaggtct 
     5701 tccaatatgt tttttaaagc gctcttgctc gacatataag tgtccgaatt ctttgcttct 
     5761 aaggacaact ttcctccaac aaatggcaag ttttccatca tttttttaga tttaactaac 
     5821 ctcatcaacg gctcgtatat ttgaaaggta aagatagggt tggggagctt tcttaagtat 
     5881 cgcttcaana tccttttgtt gtttccgggt gtacaatatg gacttcctct tttctggcaa 
     5941 ccaaacccat acatcgggat tcctataata ccttcgttgg tctccctaac atgtaggtgg 
     6001 cggaggggag atatacaata gaacagatac cagacaagac ataatgggct aaacaagact 
     6061 acaccaatta cactgcctca ttgatggtgg tacataacga actaatactg tagccctaga 
     6121 cttgatagcc atcatcatat cgaagtttca ctaccctttt tccatttgcc atctattgaa 
     6181 gtaataatag gcgcatgcaa cttcttttct ttttttttct tttctctctc ccccgttgtt 
     6241 gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga cactaaagga aaaaattaac 
     6301 gacaaagaca gcaccaacag atgtcgttgt tccagagctg atgaggggta tctcgaagca 
     6361 cacgaaactt tttccttcct tcattcacgc acactactct ctaatgagca acggtatacg 
     6421 gccttccttc cagttacttg aatttgaaat aaaaaaagtt tgccgctttg ctatcaagta 
     6481 taaatagacc tgcaattatt aatcttttgt ttcctcgtca ttgttctcgt tccctttctt 
     6541 ccttgtttct ttttctgcac aatatttcaa gctataccaa gcatacaatc aactccaagc 
     6601 ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta tcgaacaagc atgcgatatt 
     6661 tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag 
     6721 aacaactggg agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat 
     6781 ctgacagaag tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct 
     6841 cgagaagacc ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta 
     6901 acaggattat ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca 
     6961 gtggagactg atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg 
     7021 gaagagagta gtaacaaagg tcaaagacag ttgactgtat cgccggaatt catggcttac 
     7081 ccatacgatg ttccagatta cgctagcttg ggtggtcata tggccatgga ggccccgggg 
     7141 atccgtcgac ctgcagccaa gctaattccg ggcgaatttc ttatgattta tgatttttat 
     7201 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt 
     7261 aaaacgaaaa ttcttgttct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt 
     7321 atagcatgag gtcgctctta ttgaccacac ctctaccggc atgcaagctt ggcgtaatca 
     7381 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 
     7441 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt 
     7501 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct ggattaatga 
     7561 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 
     7621 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 
     7681 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 
     7741 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 
     7801 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 
     7861 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 
     7921 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 
     7981 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 
     8041 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 
     8101 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 
     8161 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 
     8221 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 
     8281 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 
     8341 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 
     8401 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 
     8461 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 
     8521 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 
     8581 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 
     8641 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 
     8701 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 
     8761 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 
     8821 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 
     8881 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 
     8941 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 
     9001 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 
     9061 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 
     9121 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 
     9181 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 
     9241 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 
     9301 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 
     9361 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 
     9421 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
     9481 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acct 
//
