ID pAS3 standard; circular DNA; ; 9534 BP. DE LMBP 4253. CC Accession number: LMBP 4253 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 8-JAN-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT rep_origin complement(2..982) FT /vntifkey="33" FT /label=2mu\ori FT CDS 3477..5192 FT /vntifkey="4" FT /label=ADE2 FT rep_origin complement(7601..8003) FT /vntifkey="33" FT /label=pMB1\ori FT terminator 7172..7273 FT /vntifkey="43" FT /label=ADH1\terminator FT misc_marker 7078..7104 FT /vntifkey="22" FT /label=HA\epitope FT CDS 6622..7062 FT /vntifkey="4" FT /label=GALbd FT promoter 6196..6599 FT /vntifkey="29" FT /label=ADH1\promoter FT CDS complement(8544..9404) FT /vntifkey="4" FT /label=ampicillin FT terminator complement(1060..1164) FT /vntifkey="43" FT /label=FLP\terminator FT promoter complement(7426..7509) FT /vntifkey="30" FT /label=lac\promoter SQ Sequence 9534 BP; 2820 A; 1970 C; 1965 G; 2777 t; 2 other; gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 60 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 120 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 180 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 240 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 300 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 360 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 420 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 480 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 540 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 600 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 660 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 720 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 780 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 840 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 900 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 960 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 1020 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 1080 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 1140 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 1200 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 1260 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 1320 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 1380 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 1440 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 1500 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 1560 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 1620 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 1680 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 1740 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 1800 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 1860 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 1920 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 1980 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 2040 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 2100 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 2160 gcattgatnc cacggtgtta tagtatcagc aatagatctt tttactcaaa tttacagttt 2220 tgatatcttg gcgtttttcc tcacatttat tacaaaagaa agtgggcagg atttgccctt 2280 cacaaaatct ctgtccagga gaagaacgta tttgaccaga attgctcaat cttcttcatt 2340 gagacagttt ttacaactga gtacacgccc taatataagg attctgggag gtaatgggca 2400 gggtactgga aattccaatt atcccgaatt tacaaacgca tcctcagtca tctcacccaa 2460 gctaagggct tctcctctac tagaaagaag ggcgtcaaaa atctgctatg ctattacgaa 2520 attactctat aggttagaat gtcatcccat aggtatggcc cttttaaaga agtatctcca 2580 caatcaattg cgagaagcct atctagaatc aaaacgacac tttatttcca aaaagggaga 2640 ttcgaccaac acttcctcta ccattgcatc atcatctttc gctggcgcat ctgttcctct 2700 atcttcaaac gaatcaggaa tgctaaacgg cttgaagcaa attaacgaac aacaagaatc 2760 tacattagaa accactcaaa aggaagacta gtaacgccgt atcgtgatta acgtattaca 2820 taagttacag gattcatgct tatgggttag ctatttcgcc caatgtgtcc atctgacatt 2880 actattttgc attttaattt aattagaact tgactagcgc actaccagta tatcatctca 2940 tttccgtaaa taccaaatgt attatatatt gaaagctttt gaccaggtta ttataaaaga 3000 aacttcatgc tcgaaaaaga tcatttcgaa aagttgccta gtttcatgaa attttaaagc 3060 agtttatata aattttacct tttgatgcgg aattgacttt ttcttgaata atacataact 3120 tttcttaaaa gaatcaaaga cagataaaat ttaagagata ttaaatatta gtgagaagcc 3180 gagaattttg taacaccaac ataacactga catctttaac aacttttaat tatgatacat 3240 ttcttacgtc atgattgatt attacagcta tgctgacaaa tgactcttgt tgcatggcta 3300 cgaaccgggt aatactaagt gattgactct tgctgacctt ttattaagaa ctaaatggac 3360 aatattatgg agcatttcat gtataaattg gtgcgtaaaa tcgttggatc tctcttctaa 3420 gtacatccta ctataacaat caagaaaaac aagaaaatcg gacaaaacaa tcaagtatgg 3480 attctagaac agttggtata ttaggagggg gacaattggg acgtatgatt gttgaggcag 3540 caaacaggct caacattaag acggtaatac tagatgctga aaattctcct gccaaacaaa 3600 taagcaactc caatgaccac gttaatggct ccttttccaa tcctcttgat atcgaaaaac 3660 tagctgaaaa atgtgatgtg ctaacgattg agattgagca tgttgatgtt cctacactaa 3720 agaatcttca agtaaaacat cccaaattaa aaatttaccc ttctccagaa acaatcagat 3780 tgatacaaga caaatatatt caaaaagagc atttaatcaa aaatggtata gcagttaccc 3840 aaagtgttcc tgtggaacaa gccagtgaga cgtccctatt gaatgttgga agagatttgg 3900 gttttccatt cgtcttgaag tcgaggactt tggcatacga tggaagaggt aacttcgttg 3960 taaagaataa ggaaatgatt ccggaagctt tggaagtact gaaggatcgt cctttgtacg 4020 ccgaaaaatg ggcaccattt actaaagaat tagcagtcat gattgtgaga tctgttaacg 4080 gtttagtgtt ttcttaccca attgtagaga ctatccacaa ggacaatatt tgtgacttat 4140 gttatgcgcc tgctagagtt ccggactccg ttcaacttaa ggcgaagttg ttggcagaaa 4200 atgcaatcaa atcttttccc ggttgtggta tatttggtgt ggaaatgttc tatttagaaa 4260 caggggaatt gcttattaac gaaattgccc caaggcctca caactctgga cattatacca 4320 ttgatgcttg cgtcacttct caatttgaag ctcatttgag atcaatattg gatttgccaa 4380 tgccaaagaa tttcacatct ttctccacca ttacaacgaa cgccattatg ctaaatgttc 4440 ttggagacaa acatacaaaa gataaagagc tagaaacttg cgaaagagca ttggcgactc 4500 caggttcctc agtgtactta tatggaaaag agtctagacc taacagaaaa gtaggtcaca 4560 taaatattat tgcctccagt atggcggaat gtgaacaaag gctgaactac attacaggta 4620 gaactgatat tccaatcaaa atctctgtcg ctcaaaagtt ggacttggaa gcaatggtca 4680 aaccattggt tggaatcatc atgggatcag actctgactt gccggtaatg tctgccgcat 4740 gtgcggtttt aaaagatttt ggcgttccat ttgaagtgac aatagtctct gctcatagaa 4800 ctccacatag gatgtcagca tatgctattt ccgcaagcaa gcgtggaatt aaaacaatta 4860 tcgctggagc tggtggggct gctcacttgc caggtatggt ggctgcaatg acaccacttc 4920 ctgtcatcgg tgtgcccgta aaaggttctt gtctagatgg agtagattct ttacattcaa 4980 ttgtgcaaat gcctagaggt gttccagtag ctaccgtcgc tattaataat agtacgaacg 5040 ctgcgctgtt ggctgtcaga ctgcttggcg cttatgattc aagttataca acgaaaatgg 5100 aacagttttt attaaagcaa gaagaagaag ttcttgtcaa agcacaaaag ttagaaactg 5160 tcggttacga agcttatcta gaaaacaagt aatatataag tttattgata tacttgtaca 5220 gcaaataatt ataaaatgat atacctattt tttaggcttt gttatgatta catcaaatgt 5280 ggacttcata catagaaatc aacgcttaca ggtgtccttt tttaagaatt tcatacataa 5340 gatcacttat tatacataca tacatatcca gtaacaagaa gcaaggaata attacctgct 5400 taagtctgcg attaaaaaaa taacgtttcg atacagttca tataaggcgg ctcaatgcag 5460 aaccgaggat agcgctacgt caggatatct ttgtagttcc caaatataaa tgcgacaata 5520 tagtttcttt ctttcatatc aataatatcc ttttctccac tgaaatcacg aatcaaacct 5580 ggagcaaaaa ctaaagccaa attataaagc gtcattcgat tccagtgact gtaccgtgta 5640 accttttcta tatgttcact cagtactctt aacaccctat aatgttccct tggaaggtct 5700 tccaatatgt tttttaaagc gctcttgctc gacatataag tgtccgaatt ctttgcttct 5760 aaggacaact ttcctccaac aaatggcaag ttttccatca tttttttaga tttaactaac 5820 ctcatcaacg gctcgtatat ttgaaaggta aagatagggt tggggagctt tcttaagtat 5880 cgcttcaana tccttttgtt gtttccgggt gtacaatatg gacttcctct tttctggcaa 5940 ccaaacccat acatcgggat tcctataata ccttcgttgg tctccctaac atgtaggtgg 6000 cggaggggag atatacaata gaacagatac cagacaagac ataatgggct aaacaagact 6060 acaccaatta cactgcctca ttgatggtgg tacataacga actaatactg tagccctaga 6120 cttgatagcc atcatcatat cgaagtttca ctaccctttt tccatttgcc atctattgaa 6180 gtaataatag gcgcatgcaa cttcttttct ttttttttct tttctctctc ccccgttgtt 6240 gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga cactaaagga aaaaattaac 6300 gacaaagaca gcaccaacag atgtcgttgt tccagagctg atgaggggta tctcgaagca 6360 cacgaaactt tttccttcct tcattcacgc acactactct ctaatgagca acggtatacg 6420 gccttccttc cagttacttg aatttgaaat aaaaaaagtt tgccgctttg ctatcaagta 6480 taaatagacc tgcaattatt aatcttttgt ttcctcgtca ttgttctcgt tccctttctt 6540 ccttgtttct ttttctgcac aatatttcaa gctataccaa gcatacaatc aactccaagc 6600 ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta tcgaacaagc atgcgatatt 6660 tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag 6720 aacaactggg agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat 6780 ctgacagaag tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct 6840 cgagaagacc ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta 6900 acaggattat ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca 6960 gtggagactg atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg 7020 gaagagagta gtaacaaagg tcaaagacag ttgactgtat cgccggaatt catggcttac 7080 ccatacgatg ttccagatta cgctagcttg ggtggtcata tggccatgga ggccccgggg 7140 atccgtcgac ctgcagccaa gctaattccg ggcgaatttc ttatgattta tgatttttat 7200 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt 7260 aaaacgaaaa ttcttgttct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt 7320 atagcatgag gtcgctctta ttgaccacac ctctaccggc atgcaagctt ggcgtaatca 7380 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 7440 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt 7500 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct ggattaatga 7560 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 7620 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 7680 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 7740 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 7800 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 7860 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 7920 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 7980 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 8040 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 8100 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 8160 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 8220 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 8280 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 8340 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 8400 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 8460 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 8520 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 8580 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 8640 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 8700 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 8760 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 8820 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 8880 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 8940 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 9000 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 9060 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 9120 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 9180 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 9240 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 9300 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 9360 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 9420 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 9480 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acct 9534 //