LOCUS       pCAGGS-mCASP-1p10HA        5058 bp    DNA     circular     26-OCT-2007
DEFINITION  LMBP 4347.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4347
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 26-OCT-2007
            
COMMENT     VNTDATE|440172089|
COMMENT     VNTNAME|pCAGGS-mCASP-1p10HA|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-mCASP-1p10HA" 0 5058 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "EcoRI" "XhoI" "MscI" "SmaI" "BsrDI" "HindIII" "NotI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5058 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 5057 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 5 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "MscI" "tggcca" 4 0 1 319 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 4 434 1 2470 0 2644 1 4678 
COMMENT            1 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 814 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 280 0 0 "")) 
COMMENT       (CObList 
COMMENT        #7=(CFSignal (CObList) "HA epitope" 22 0 0 286 312 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "286..312") 
COMMENT        #8=(CFSignal (CObList) "\337-globin polyA" 43 0 0 348 439 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "348..439") 
COMMENT        #9=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 440 796 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "440..796") 
COMMENT        #10=(CFSignal (CObList) "lac promoter" 30 0 1 874 956 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(874..956)") 
COMMENT        #11=(CFSignal (CObList) "SV40 ori" 33 0 0 1098 1173 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1098..1173") 
COMMENT        #12=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1204 1278 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1204..1278") 
COMMENT        #13=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1204 1278 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1204..1278)") 
COMMENT        #14=(CFSignal (CObList) "pMB1 ori" 33 0 1 1393 1794 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1393..1794)") 
COMMENT        #15=(CFSignal (CObList) "ampicillin" 4 0 1 2335 3195 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2335..3195)") 
COMMENT        #16=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3331 3708 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3331..3708") 
COMMENT        #17=(CFSignal (CObList) "AG promoter" 29 0 0 3714 5055 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3714..5055") 
COMMENT        #18=(CFSignal (CObList) "intron AG promoter" 15 0 0 4083 5007 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4083..5007") 
COMMENT        #19=(CFSignal (CObList) "mCASP-1, p10 subunit" 4 0 0 10 276 0 
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COMMENT       (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #20=(CFSignal (CObList) "LMBP 4347" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #21=(CFSignal (CObList) "Last modified: 26-OCT-2007" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pCAGGS-mCASP-1p10HA'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 03/06/07 02:01" 1) 
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COMMENT                          "Last Modified: 10/26/07 11:55" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5058 bp" 1) 
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COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 6-MAR-2007" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "margotbaetens" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:margot.baetens@dmbr.ugent.be\">margot.baetens@dmbr.ugent.be</a>" 
COMMENT                          1))) "Author") 
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COMMENT                                 "" 1))) "LMBP 4347") 
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COMMENT                                 "ampicillin"))) "CDS (2 total)") 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                        "   complement(874..956)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                            "GCAATGNNN" "CGTTACNNN") 
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FEATURES             Location/Qualifiers
     CDS             10..276
                     /vntifkey="4"
                     /label=mCASP-1,\p10\subunit
                     /note="mouse caspase-1 p10 subunit"
     misc_marker     286..312
                     /vntifkey="22"
                     /label=HA\epitope
                     /note="Influenza HA epitope"
     terminator      348..439
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     misc_feature    440..796
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     promoter        complement(874..956)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      1098..1173
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      1204..1278
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(1204..1278)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(1393..1794)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(2335..3195)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     promoter        3331..3708
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     promoter        3714..5055
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     intron          4083..5007
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid intron"
BASE COUNT     1079 a      1332 c      1383 g      1264 t 
ORIGIN
        1 ttcctcgaga tgggcattaa gaaggcccat atagagaaag attttattgc tttctgctct 
       61 tcaacaccag ataatgtgtc ttggagacat cctgtcaggg gctcactttt cattgagtca 
      121 ctcatcaaac acatgaaaga atatgcctgg tcttgtgact tggaggacat tttcagaaag 
      181 gttcgatttt catttgaaca accagaattt aggctacaga tgcccactgc tgatagggtg 
      241 accctgacaa aacgtttcta cctcttcccc ggacatagcg gccgctaccc atacgatgtt 
      301 ccagattacg cttaatggcc aatgccctgg ctcacaaata ccactgagat ctttttccct 
      361 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa 
      421 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga 
      481 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa 
      541 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata 
      601 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga 
      661 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac 
      721 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc 
      781 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg 
      841 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 
      901 aagtgtaaag cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
      961 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc 
     1021 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 
     1081 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 
     1141 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 
     1201 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 
     1261 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 
     1321 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 
     1381 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 
     1441 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga 
     1501 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 
     1561 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 
     1621 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 
     1681 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 
     1741 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 
     1801 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 
     1861 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 
     1921 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 
     1981 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 
     2041 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
     2101 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
     2161 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 
     2221 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 
     2281 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 
     2341 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
     2401 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 
     2461 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 
     2521 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 
     2581 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 
     2641 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 
     2701 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 
     2761 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 
     2821 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 
     2881 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 
     2941 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 
     3001 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 
     3061 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 
     3121 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 
     3181 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 
     3241 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 
     3301 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt 
     3361 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta 
     3421 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga 
     3481 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt 
     3541 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta 
     3601 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg 
     3661 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga 
     3721 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt 
     3781 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc 
     3841 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag 
     3901 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 
     3961 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc 
     4021 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac 
     4081 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac 
     4141 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt 
     4201 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg 
     4261 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc 
     4321 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg 
     4381 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg 
     4441 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg 
     4501 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt 
     4561 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg 
     4621 aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat 
     4681 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg 
     4741 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc 
     4801 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc 
     4861 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg 
     4921 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat 
     4981 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct 
     5041 catcattttg gcaaagaa 
//
