LOCUS       pCAGGS-EmCASP-1prop20m3 5771 bp dsDNACircular
DEFINITION  LMBP 4349.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     CDS             61..945
                     /label=mCASP-1prop20\C285S\mutant
     CDS             complement(3048..3908)
                     /label=AMP
     promoter        4044..4421
                     /label=hCMV\enhancer
     promoter        4427..5768
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
                     /label=AG\promoter
     misc_difference 16..54
                     /label=E-tag
     terminator      1061..1152
                     /label=ß-globin\polyA
     promoter        complement(1587..1669)
                     /label=lac\promoter
     rep_origin      1811..1886
                     /label=SV40\ori
     terminator      1916..1991
                     /label=SV40\polyA\early
     terminator      complement(1916..1991)
                     /label=SV40\polyA\late
     rep_origin      complement(2106..2507)
                     /label=pMB1\ori
     intron          4796..5720
                     /label=intron\AG\promoter
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gctgacaaga tcctgagggc aaagaggaag caatttatca actcagtgag tatagggaca 
      121 ataaatggat tgttggatga acttttagag aagagagtgc tgaatcagga agaaatggat 
      181 aaaataaaac ttgcaaacat tactgctatg gacaaggcac gggacctatg tgatcatgtc 
      241 tctaaaaaag ggccccaggc aagccaaatc tttatcactt acatttgtaa tgaagactgc 
      301 tacctggcag gaattctgga gcttcaatca gctccatcag ctgaaacatt tgttgctaca 
      361 gaagattcta aaggaggaca tccttcatcc tcagaaacaa aggaagaaca gaacaaagaa 
      421 gatggcacat ttccaggact gactgggacc ctcaagtttt gccctttaga aaaagcccag 
      481 aagttatgga aagaaaatcc ttcagagatt tatccaataa tgaatacaac cactcgtaca 
      541 cgtcttgccc tcattatctg caacacagag tttcaacatc tttctccgag ggttggagct 
      601 caagttgacc tcagagaaat gaagttgctg ctggaggatc tggggtatac cgtgaaagtg 
      661 aaagaaaatc tcacagctct ggagatggtg aaagaggtga aagaatttgc tgcctgccca 
      721 gagcacaaga cttctgacag tactttcctt gtattcatgt ctcatggtat ccaggaggga 
      781 atatgtggga ccacatactc taatgaagtt tcagatattt taaaggttga cacaatcttt 
      841 cagatgatga acactttgaa gtgcccaagc ttgaaagaca agcccaaggt gatcattatt 
      901 caagctagcc gtggagagaa acaaggagtg gtgttgttaa aataacccgg gacattaaac 
      961 gcgtgggtac cggatccggc aattcactcc tcaggtgcag gctgcctatc agaaggtggt 
     1021 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa 
     1081 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt 
     1141 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg 
     1201 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc 
     1261 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca 
     1321 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt 
     1381 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt 
     1441 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt 
     1501 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg 
     1561 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 
     1621 aagcctgggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 
     1681 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata 
     1741 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 
     1801 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 
     1861 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact 
     1921 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 
     1981 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 
     2041 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 
     2101 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
     2161 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag ggataacgca 
     2221 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 
     2281 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 
     2341 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 
     2401 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 
     2461 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 
     2521 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 
     2581 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 
     2641 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 
     2701 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 
     2761 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 
     2821 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 
     2881 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     2941 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 
     3001 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 
     3061 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 
     3121 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 
     3181 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 
     3241 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 
     3301 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 
     3361 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 
     3421 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 
     3481 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 
     3541 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 
     3601 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 
     3661 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 
     3721 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 
     3781 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 
     3841 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 
     3901 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 
     3961 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
     4021 ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat 
     4081 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 
     4141 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 
     4201 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 
     4261 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 
     4321 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 
     4381 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac 
     4441 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 
     4501 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcccc ccccaggcgg 
     4561 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca 
     4621 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 
     4681 aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc 
     4741 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag 
     4801 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt 
     4861 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg 
     4921 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc 
     4981 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt 
     5041 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa 
     5101 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt 
     5161 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg 
     5221 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca 
     5281 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaaggggc 
     5341 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt 
     5401 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga 
     5461 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg 
     5521 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc 
     5581 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg 
     5641 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg 
     5701 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt 
     5761 ttggcaaaga a 
//
