LOCUS       pCAGGS-EmCASP-1prop20 5774 bp dsDNACircular
DEFINITION  LMBP 4350.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     promoter        4047..4424
                     /label=hCMV\enhancer
     promoter        4430..5771
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
                     /label=AG\promoter
     rep_origin      complement(2109..2510)
                     /label=pMB1\ori
     CDS             complement(3051..3911)
                     /label=AMP
     misc_difference 16..54
                     /label=E-tag
     terminator      1064..1155
                     /label=ß-globin\polyA
     promoter        complement(1590..1672)
                     /label=lac\promoter
     rep_origin      1814..1889
                     /label=SV40\ori
     terminator      1919..1994
                     /label=SV40\polyA\early
     terminator      complement(1919..1994)
                     /label=SV40\polyA\late
     intron          4799..5723
                     /label=intron\AG\promoter
     CDS             64..948
                     /label=mCASP-1prop20
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gctgctgaca agatcctgag ggcaaagagg aagcaattta tcaactcagt gagtataggg 
      121 acaataaatg gattgttgga tgaactttta gagaagagag tgctgaatca ggaagaaatg 
      181 gataaaataa aacttgcaaa cattactgct atggacaagg cacgggacct atgtgatcat 
      241 gtctctaaaa aagggcccca ggcaagccaa atctttatca cttacatttg taatgaagac 
      301 tgctacctgg caggaattct ggagcttcaa tcagctccat cagctgaaac atttgttgct 
      361 acagaagatt ctaaaggagg acatccttca tcctcagaaa caaaggaaga acagaacaaa 
      421 gaagatggca catttccagg actgactggg accctcaagt tttgcccttt agaaaaagcc 
      481 cagaagttat ggaaagaaaa tccttcagag atttatccaa taatgaatac aaccactcgt 
      541 acacgtcttg ccctcattat ctgcaacaca gagtttcaac atctttctcc gagggttgga 
      601 gctcaagttg acctcagaga aatgaagttg ctgctggagg atctggggta taccgtgaaa 
      661 gtgaaagaaa atctcacagc tctggagatg gtgaaagagg tgaaagaatt tgctgcctgc 
      721 ccagagcaca agacttctga cagtactttc cttgtattca tgtctcatgg tatccaggag 
      781 ggaatatgtg ggaccacata ctctaatgaa gtttcagata ttttaaaggt tgacacaatc 
      841 tttcagatga tgaacacttt gaagtgccca agcttgaaag acaagcccaa ggtgatcatt 
      901 attcaggcat gccgtggaga gaaacaagga gtggtgttgt taaaataacc cgggacatta 
      961 aacgcgtggg taccggatcc ggcaattcac tcctcaggtg caggctgcct atcagaaggt 
     1021 ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc 
     1081 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa 
     1141 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata 
     1201 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata 
     1261 tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa 
     1321 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt 
     1381 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt 
     1441 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct 
     1501 cttatggaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc 
     1561 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 
     1621 gtaaagcctg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 
     1681 cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc 
     1741 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct 
     1801 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 
     1861 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta 
     1921 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 
     1981 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 
     2041 atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 
     2101 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 
     2161 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat cagggataac 
     2221 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 
     2281 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 
     2341 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 
     2401 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 
     2461 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 
     2521 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 
     2581 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 
     2641 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 
     2701 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 
     2761 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 
     2821 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 
     2881 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 
     2941 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 
     3001 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 
     3061 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 
     3121 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 
     3181 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 
     3241 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 
     3301 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 
     3361 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 
     3421 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 
     3481 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 
     3541 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 
     3601 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 
     3661 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 
     3721 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 
     3781 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 
     3841 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 
     3901 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 
     3961 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 
     4021 tttccccgaa aagtgccacc tgggtcgaca ttgattattg actagttatt aatagtaatc 
     4081 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt 
     4141 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 
     4201 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 
     4261 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 
     4321 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 
     4381 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc 
     4441 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt 
     4501 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc cccccccagg 
     4561 cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg aaaggtgcgg cggcagccaa 
     4621 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta 
     4681 taaaaagcga agcgcgcggc gggcgggagt cgttgcgcgc tgccttcccc ccgtgccccg 
     4741 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt 
     4801 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct 
     4861 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg 
     4921 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc 
     4981 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag 
     5041 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg 
     5101 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc 
     5161 ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc 
     5221 gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg 
     5281 gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaagg 
     5341 ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc 
     5401 ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc 
     5461 cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg 
     5521 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc 
     5581 tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca 
     5641 gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc 
     5701 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc 
     5761 attttggcaa agaa 
//
