LOCUS       pCAGGS-EmCASP-1p20 5411 bp dsDNACircular
DEFINITION  LMBP 4352.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     promoter        3684..4061
                     /label=hCMV\enhancer
     promoter        4067..5408
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
                     /label=AG\promoter
     misc_difference 16..54
                     /label=E-tag
     terminator      701..792
                     /label=ß-globin\polyA
     promoter        complement(1227..1309)
                     /label=lac\promoter
     rep_origin      1451..1526
                     /label=SV40\ori
     terminator      1556..1631
                     /label=SV40\polyA\early
     terminator      complement(1556..1631)
                     /label=SV40\polyA\late
     CDS             complement(2688..3548)
                     /label=AMP
     rep_origin      complement(1746..2147)
                     /label=pMB1\ori
     intron          4436..5360
                     /label=intron\AG\promoter
     CDS             64..585
                     /label=mCASP-1p20
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gctggcacat ttccaggact gactgggacc ctcaagtttt gccctttaga aaaagcccag 
      121 aagttatgga aagaaaatcc ttcagagatt tatccaataa tgaatacaac cactcgtaca 
      181 cgtcttgccc tcattatctg caacacagag tttcaacatc tttctccgag ggttggagct 
      241 caagttgacc tcagagaaat gaagttgctg ctggaggatc tggggtatac cgtgaaagtg 
      301 aaagaaaatc tcacagctct ggagatggtg aaagaggtga aagaatttgc tgcctgccca 
      361 gagcacaaga cttctgacag tactttcctt gtattcatgt ctcatggtat ccaggaggga 
      421 atatgtggga ccacatactc taatgaagtt tcagatattt taaaggttga cacaatcttt 
      481 cagatgatga acactttgaa gtgcccaagc ttgaaagaca agcccaaggt gatcattatt 
      541 caggcatgcc gtggagagaa acaaggagtg gtgttgttaa aataacccgg gacattaaac 
      601 gcgtgggtac cggatccggc aattcactcc tcaggtgcag gctgcctatc agaaggtggt 
      661 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa 
      721 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt 
      781 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg 
      841 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc 
      901 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca 
      961 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt 
     1021 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt 
     1081 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt 
     1141 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg 
     1201 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 
     1261 aagcctgggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 
     1321 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata 
     1381 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 
     1441 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 
     1501 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact 
     1561 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 
     1621 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 
     1681 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 
     1741 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
     1801 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag ggataacgca 
     1861 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 
     1921 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 
     1981 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 
     2041 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 
     2101 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 
     2161 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 
     2221 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 
     2281 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 
     2341 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 
     2401 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 
     2461 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 
     2521 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     2581 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 
     2641 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 
     2701 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 
     2761 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 
     2821 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 
     2881 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 
     2941 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 
     3001 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 
     3061 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 
     3121 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 
     3181 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 
     3241 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 
     3301 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 
     3361 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 
     3421 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 
     3481 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 
     3541 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 
     3601 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
     3661 ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat 
     3721 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 
     3781 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 
     3841 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 
     3901 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 
     3961 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 
     4021 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac 
     4081 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 
     4141 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcccc ccccaggcgg 
     4201 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca 
     4261 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 
     4321 aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc 
     4381 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag 
     4441 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt 
     4501 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg 
     4561 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc 
     4621 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt 
     4681 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa 
     4741 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt 
     4801 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg 
     4861 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca 
     4921 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaaggggc 
     4981 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt 
     5041 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga 
     5101 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg 
     5161 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc 
     5221 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg 
     5281 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg 
     5341 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt 
     5401 ttggcaaaga a 
//
