LOCUS       pCAGGSE-mIL1B-HA        5723 bp    DNA     circular      5-DEC-2013
DEFINITION  LMBP 4354.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4354
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 05-NOV-2013
COMMENT     VNTDATE|677323386|
COMMENT     VNTNAME|pCAGGSE-mIL1B-HA|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGSE-mIL1B-HA" 0 5723 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BamHI" "BglII" "EcoRI" "HincII" "HindIII" "SacII" "NotI" 
COMMENT         "XmaI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 
COMMENT        0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 877 1140 1 5723 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 4 667 0 925 0 1663 0 2000 
COMMENT            0 0 "") #1=(CRSite (CStringList) "BglII" "agatct" 2 0 1 1013 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HincII" "gtyrac" 4 0 2 136 0 3996 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1479 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 5028 0 5557 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 899 0 0 "")) 
COMMENT       (CObList 
COMMENT        #8=(CFSignal (CObList) "mIL1B" 4 0 0 64 867 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "64..867") 
COMMENT        #9=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT            0 1013 1104 0 (CStringList) (CStringList) 1 1 1 1 "930..1021") 
COMMENT        #10=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
COMMENT             0 1105 1461 0 (CStringList) (CStringList) 1 1 1 1 "1022..1378") 
COMMENT        #11=(CFSignal (CObList) "lac promoter" 30 0 1 1539 1621 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1456..1538)") 
COMMENT        #12=(CFSignal (CObList) "SV40 ori" 33 0 0 1763 1838 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1680..1755") 
COMMENT        #13=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1869 1943 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1786..1860") 
COMMENT        #14=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1869 1943 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1786..1860)") 
COMMENT        #15=(CFSignal (CObList) "pMB1 ori" 33 0 1 2058 2459 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1975..2376)") 
COMMENT        #16=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3000 3860 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2917..3777)") 
COMMENT        #17=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3996 4373 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3913..4290") 
COMMENT        #18=(CFSignal (CObList) "AG promoter" 29 0 0 4379 5720 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4296..5637") 
COMMENT        #19=(CFSignal (CObList) "intron AG promoter" 15 0 0 4748 5672 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4665..5589") 
COMMENT        #20=(CFSignal (CObList) "HA epitope" 22 0 0 868 894 0 (CStringList) 
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COMMENT        #21=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
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COMMENT       (CObList) 
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COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #23=(CFSignal (CObList) "Last modified: 05-NOV-2013" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pCAGGSE-mIL1B-HA'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 10/31/13 09:23" 1) 
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COMMENT                          "Last Modified: 12/05/13 10:43" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5723 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 5-NOV-2013" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "Misc. Marker (2 total)") 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                        "   complement(1456..1538)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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FEATURES             Location/Qualifiers
     CDS             64..867
                     /vntifkey="4"
                     /label=mIL1B
     terminator      1013..1104
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_feature    1105..1461
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     promoter        complement(1539..1621)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      1763..1838
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      1869..1943
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(1869..1943)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(2058..2459)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(3000..3860)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        3996..4373
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     promoter        4379..5720
                     /vntifkey="29"
                     /label=AG\promoter
     intron          4748..5672
                     /vntifkey="15"
                     /label=intron\AG\promoter
     misc_marker     868..894
                     /vntifkey="22"
                     /label=HA\epitope
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
BASE COUNT     1260 a      1511 c      1557 g      1395 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gctgcaactg ttcctgaact caactgtgaa atgccacctt ttgacagtga tgagaatgac 
      121 ctgttctttg aagttgacgg accccaaaag atgaagggct gcttccaaac ctttgacctg 
      181 ggctgtcctg atgagagcat ccagcttcaa atctcgcagc agcacatcaa caagagcttc 
      241 aggcaggcag tatcactcat tgtggctgtg gagaagctgt ggcagctacc tgtgtctttc 
      301 ccgtggacct tccaggatga ggacatgagc accttctttt ccttcatctt tgaagaagag 
      361 cccatcctct gtgactcatg ggatgatgat gataacctgc tggtgtgtga cgttcccatt 
      421 agacaactgc actacaggct ccgagatgaa caacaaaaaa gcctcgtgct gtcggaccca 
      481 tatgagctga aagctctcca cctcaatgga cagaatatca accaacaagt gatattctcc 
      541 atgagctttg tacaaggaga accaagcaac gacaaaatac ctgtggcctt gggcctcaaa 
      601 ggaaagaatc tacacctgtc ctgtgtaatg aaagacggca cacccaccct gcagctggag 
      661 agtgtggatc ccaagcaata cccaaagaag aagatggaaa aacggtttgt cttcaacaag 
      721 atagaagtca agagcaaagt ggagtttgag tctgcagagt tccccaactg gtacatcagc 
      781 acctcacaag cagagcacaa gcctgtcttc ctgggaaaca acagtggtca ggacataatt 
      841 gacttcacca tggaatctgt gtcttcctac ccatacgatg ttccagatta cgcttaaccc 
      901 gggacattaa acgcgtgggt accggatccg gcaattcact cctcaggtgc aggctgccta 
      961 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt 
     1021 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta 
     1081 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg 
     1141 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt 
     1201 ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca 
     1261 gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg 
     1321 ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc 
     1381 cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt 
     1441 ccctcttctc ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat 
     1501 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 
     1561 gcataaagtg taaagcctgg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 
     1621 ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag 
     1681 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 
     1741 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 
     1801 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 
     1861 aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 
     1921 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 
     1981 atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg 
     2041 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 
     2101 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 
     2161 agggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 
     2221 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 
     2281 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 
     2341 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 
     2401 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 
     2461 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 
     2521 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 
     2581 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 
     2641 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 
     2701 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 
     2761 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 
     2821 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 
     2881 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 
     2941 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 
     3001 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 
     3061 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 
     3121 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 
     3181 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 
     3241 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 
     3301 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 
     3361 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 
     3421 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 
     3481 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 
     3541 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 
     3601 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 
     3661 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 
     3721 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 
     3781 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 
     3841 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 
     3901 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 
     3961 ccgcgcacat ttccccgaaa agtgccacct gggtcgacat tgattattga ctagttatta 
     4021 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 
     4081 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 
     4141 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 
     4201 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 
     4261 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 
     4321 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga 
     4381 ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt 
     4441 gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcc 
     4501 ccccccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga aaggtgcggc 
     4561 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg 
     4621 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gttgcgcgct gccttccccc 
     4681 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact 
     4741 cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta 
     4801 atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc 
     4861 tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc 
     4921 gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc 
     4981 gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg 
     5041 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt 
     5101 gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg 
     5161 cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg 
     5221 ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc 
     5281 gggggaaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca 
     5341 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct 
     5401 gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc 
     5461 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc 
     5521 gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg 
     5581 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta 
     5641 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc 
     5701 tgtctcatca ttttggcaaa gaa 
//
