LOCUS       pAS104                  4307 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 4356.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pAS104|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAS104" 0 4307 
COMMENT       (CDBMol 0 0 1 1 1 0 0 2228321 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 4307 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "lac" 30 0 0 95 178 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "95..178") 
COMMENT        #4=(CFSignal (CObList) "SP6" 30 0 0 239 255 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "239..255") 
COMMENT        #5=(CFSignal (CObList) "E35S" 30 0 1 336 694 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(336..694)") 
COMMENT        #6=(CFSignal (CObList) "T7" 30 0 1 766 782 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "complement(766..782)") 
COMMENT        #7=(CFSignal (CObList) "f1ORI" 33 0 0 947 1360 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "947..1360") 
COMMENT        #8=(CFSignal (CObList) "NEO" 4 0 0 1695 2489 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1695..2489") 
COMMENT        #9=(CFSignal (CObList) "AMP" 4 0 0 2507 3367 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2507..3367") 
COMMENT        #10=(CFSignal (CObList) "ORI" 33 0 0 3908 4307 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3908..4307")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
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COMMENT             (CObjectList 
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COMMENT                          "DNA 'pAS104'" 1) 
COMMENT                     #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4307 bp" 1) 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                   "Annotations") 
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COMMENT                                   #28=(CLinePar 
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COMMENT                                        "Start: 1695  End: 2489" 1) 
COMMENT                                   #29=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Tn5 antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #30=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #31=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1695..2489" 1))) "NEO") 
COMMENT                            #32=(CFolderPar 
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COMMENT                                        "Start: 2507  End: 3367" 1) 
COMMENT                                   #34=(CLinePar 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
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COMMENT                          "CDS (2 total)") 
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COMMENT                                        "Start: 95    End: 178 " 1) 
COMMENT                                   #40=(CLinePar 
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COMMENT                                        "E. coli lac operon promoter" 1) 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        "Start: 239   End: 255 " 1) 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "phage SP6 promoter" 1) 
COMMENT                                   #46=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        "Start: 336   End: 694  (Complementary)" 
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COMMENT                                        "E35S promoter/plant junction region" 1) 
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COMMENT                                        "Start: 766   End: 782  (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #55=(CLinePar 
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COMMENT                                        "phage T7 gene 10 promoter" 1) 
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COMMENT                          "Promoter Prokaryotic (4 total)") 
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COMMENT                          "Replication Origin (2 total)"))) "Feature Map")))) 
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FEATURES             Location/Qualifiers
     promoter        95..178
                     /vntifkey="30"
                     /label=lac
                     /note="E. coli lac operon promoter"
     promoter        239..255
                     /vntifkey="30"
                     /label=SP6
                     /note="phage SP6 promoter"
     promoter        complement(336..694)
                     /vntifkey="30"
                     /label=E35S
                     /note="E35S promoter/plant junction region"
     promoter        complement(766..782)
                     /vntifkey="30"
                     /label=T7
                     /note="phage T7 gene 10 promoter"
     rep_origin      947..1360
                     /vntifkey="33"
                     /label=f1ORI
                     /note="phage f1 origin"
     CDS             1695..2489
                     /vntifkey="4"
                     /label=NEO
                     /note="Tn5 antibiotic resistance gene; coding sequence"
     CDS             2507..3367
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      3908..4307
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
BASE COUNT     1038 a      1090 c      1137 g      1042 t 
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat 
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca 
      301 ctagtaacgg ccgccagtgt gctggaattc ggcttccttc aatttaaccg atgctaatga 
      361 gttatttttg catgctttaa tttgtttcta tcaaatgttt attttttttt actagaaata 
      421 acttattgca tttcattcaa aataagatca tacatacagg ttaaaataaa catagggaac 
      481 ccaaatggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat 
      541 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcac 
      601 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc 
      661 actgacgtaa gggatgacgc acaatcccac tatcaagccg aattctgcag atatccatca 
      721 cactggcggc cgctcgagca tgcatctaga gggcccaatt cgccctatag tgagtcgtat 
      781 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 
      841 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 
      901 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc 
      961 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 
     1021 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 
     1081 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagagc tttacggcac 
     1141 ctcgaccgca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag 
     1201 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 
     1261 actggaacaa cactcaaccc tatcgcggtc tattcttttg atttataagg gattttgccg 
     1321 atttcggcct attggttaaa aaatgagctg atttaacaaa ttcagggcgc aagggctgct 
     1381 aaaggaaccg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg 
     1441 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt 
     1501 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg 
     1561 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg 
     1621 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat 
     1681 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 
     1741 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 
     1801 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 
     1861 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 
     1921 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 
     1981 aagtgccggg gcaggatctc ctgtcatctc gccttgctcc tgccgagaaa gtatccatca 
     2041 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 
     2101 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 
     2161 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 
     2221 cgcgcatgcc cgacggcgag gatctcgtcg tgatccatgg cgatgcctgc ttgccgaata 
     2281 tcatggtgga aaatggccgc ttttctggat tcaacgactg tggccggctg ggtgtggcgg 
     2341 accgctatca ggacatagcg ttggataccc gtgatattgc tgaagagctt ggcggcgaat 
     2401 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 
     2461 tctatcgcct tcttgacgag ttcttctgaa ttgaaaaagg aagagtatga gtattcaaca 
     2521 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 
     2581 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 
     2641 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 
     2701 aatgatgagc acttttaaag ttctgctatg tgatacacta ttatcccgta ttgacgccgg 
     2761 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 
     2821 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 
     2881 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 
     2941 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 
     3001 ggagctgaat gaagccatac caaacgacga gagtgacacc acgatgcctg tagcaatgcc 
     3061 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 
     3121 aatagactga atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 
     3181 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 
     3241 agcactgggg ccagatggta agcgctcccg tatcgtagtt atctacacga cggggagtca 
     3301 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 
     3361 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 
     3421 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 
     3481 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 
     3541 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 
     3601 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 
     3661 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa 
     3721 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 
     3781 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 
     3841 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 
     3901 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 
     3961 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 
     4021 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 
     4081 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 
     4141 ggccttttta cggttcctgg gcttttgctg gccttttgct cacatgttct ttcctgcgtt 
     4201 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 
     4261 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag 
//
