LOCUS       pCAGGS-E-mABIN-2        6071 bp    DNA     circular     24-OCT-2007
DEFINITION  LMBP 4370.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4370
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 24-OCT-2007
COMMENT     VNTNAME|pCAGGS-E-mABIN-2|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-mABIN-2" 0 6071 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) (CStringList "BglII" "StyI" "XhoI" "EcoRI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 6071 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BglII" "agatct" 2 0 1 1361 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "StyI" "ccwwgg" 2 0 5 83 0 507 0 2104 0 2197 0 
COMMENT            4720 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 5 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 53 0 6070 0 0 "")) 
COMMENT       (CObList 
COMMENT        #4=(CFSignal (CObList) "AG promoter" 29 0 0 4727 6068 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4727..6068") 
COMMENT        #5=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 1453 1809 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1453..1809") 
COMMENT        #6=(CFSignal (CObList) "E-tag" 22 0 0 13 51 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "13..51") 
COMMENT        #7=(CFSignal (CObList) "mABIN-2" 4 0 0 67 1359 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "67..1359") 
COMMENT        #8=(CFSignal (CObList) "\337-globin polyA" 43 0 0 1361 1452 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1361..1452") 
COMMENT        #9=(CFSignal (CObList) "lac promoter" 30 0 1 1887 1969 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1887..1969)") 
COMMENT        #10=(CFSignal (CObList) "SV40 ori" 33 0 0 2111 2186 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2111..2186") 
COMMENT        #11=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2216 2291 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2216..2291") 
COMMENT        #12=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2216 2291 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2216..2291)") 
COMMENT        #13=(CFSignal (CObList) "pMB1 ori" 33 0 1 2406 2807 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2406..2807)") 
COMMENT        #14=(CFSignal (CObList) "ampicillin" 4 0 1 3348 4208 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3348..4208)") 
COMMENT        #15=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4344 4721 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4344..4721") 
COMMENT        #16=(CFSignal (CObList) "intron AG promoter" 15 0 0 5096 6020 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5096..6020")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #17=(CFSignal (CObList) "LMBP 4370" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #18=(CFSignal (CObList) "Last modified: 24-OCT-2007" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT             (CObjectList 
COMMENT              #20=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pCAGGS-E-mABIN-2" 
COMMENT                    2) 5 "" 0 4) 
COMMENT              #21=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pCAGGS-E-mABIN-2'" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4370." 
COMMENT                          1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/24/07 12:15" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6071 bp" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #30=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 25-APR-2007" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #33=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #34=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #35=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "LMBP 4370") 
COMMENT                     #37=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #38=(CLinePar (CParagraph 0 (15 #18# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 24-OCT-2007"))) 
COMMENT                   "Annotations") 
COMMENT              #39=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #40=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #41=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 67 (3 #7# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #42=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 67    End: 1359" 1) 
COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Mouse A20-binding inhibitor of NF-kappaB activation 2; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   67..1359" 1))) "mABIN-2") 
COMMENT                            #46=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3348 (3 #14# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3348  End: 4208 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3348..4208)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (2 total)") 
COMMENT                     #51=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 15 (7 15 0) 1 2 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #52=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5096  End: 6020" 1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "intron of the chicken \337-actin/rabbit \337-globin hybrid promoter" 
COMMENT                                        1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5096..6020" 1))) 
COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
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COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #58=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1453  End: 1809" 1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "3' flanking region of the  \337-globin gene" 
COMMENT                                        1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1453..1809" 1))) 
COMMENT                                 "3' FR of \337-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #63=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #64=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 13 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 13    End: 51  " 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) "E-tag" 
COMMENT                                        1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   13..51" 1))) "E-tag"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #69=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #70=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4344 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4344  End: 4721" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only" 
COMMENT                                        1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4344..4721" 1))) "hCMV-IE enhancer") 
COMMENT                            #75=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4727 (3 #4# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4727  End: 6068" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
COMMENT                                        1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4727..6068" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #80=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #81=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1887 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1887  End: 1969 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Escherichia coli lac operon promoter" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1887..1969)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #86=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #87=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2111 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2111  End: 2186" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "bidirectional origin of SV40" 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2111..2186" 1))) "SV40 ori") 
COMMENT                            #92=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2406 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2406  End: 2807 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "origin plasmid" 1) 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2406..2807)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
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COMMENT                          (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1361  End: 1452" 1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Rabbit \337-globin polyadenylation signal" 
COMMENT                                         1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #102=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   1361..1452" 1))) 
COMMENT                                 "\337-globin polyA") 
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COMMENT                                   (CObjectList 
COMMENT                                    #104=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 2216  End: 2291" 1) 
COMMENT                                    #105=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Simian virus 40 early polyadenylation signal " 
COMMENT                                          1) 
COMMENT                                    #106=(CLinePar 
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COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #107=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   2216..2291" 1))) 
COMMENT                                  "SV40 polyA early") 
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COMMENT                                   (CObjectList 
COMMENT                                    #109=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 2216  End: 2291 (Complementary)" 
COMMENT                                          1) 
COMMENT                                    #110=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Simian virus 40 late polyadenylation signal " 
COMMENT                                          1) 
COMMENT                                    #111=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #112=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(2216..2291)" 1))) 
COMMENT                                  "SV40 polyA late"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                                            " N1: 1361 " 1))))) "BglII: 1 site") 
COMMENT                            1 5 "AGATCT" "TCTAGA") 
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COMMENT                                            " N1: 53 " 1) 
COMMENT                                      #120=(CLinePar 
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COMMENT                                            " N2: 6070 " 1))))) "EcoRI: 2 sites") 
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COMMENT                                            " N1: 83 " 1) 
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COMMENT                                            " N2: 507 " 1) 
COMMENT                                      #125=(CLinePar 
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COMMENT                                            " N3: 2104 " 1) 
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COMMENT                                            " N4: 2197 " 1) 
COMMENT                                      #127=(CLinePar 
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COMMENT                                            " N5: 4720 " 1))))) "StyI: 5 sites") 
COMMENT                            1 5 "CCWWGG" "GGWWCC") 
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COMMENT                                            " N1: 5 " 1))))) "XhoI: 1 site") 1 5 
COMMENT                            "CTCGAG" "GAGCTC"))) "Restriction/Methylation Map")))) 
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COMMENT                1 2 "?" 0) 
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FEATURES             Location/Qualifiers
     terminator      1361..1452
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="Rabbit ß-globin polyadenylation signal"
     misc_marker     13..51
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     promoter        complement(1887..1969)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="Escherichia coli lac operon promoter"
     terminator      2216..2291
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="Simian virus 40 early polyadenylation signal"
     terminator      complement(2216..2291)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="Simian virus 40 late polyadenylation signal"
     rep_origin      complement(2406..2807)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     promoter        4344..4721
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only"
     rep_origin      2111..2186
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     intron          5096..6020
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="intron of the chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4727..6068
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     CDS             complement(3348..4208)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     CDS             67..1359
                     /vntifkey="4"
                     /label=mABIN-2
                     /note="Mouse A20-binding inhibitor of NF-kappaB activation 2; coding sequence"
     misc_feature    1453..1809
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the  ß-globin gene"
BASE COUNT     1329 a      1582 c      1753 g      1407 t 
ORIGIN
        1 ttcctcgaga tgggtgcgcc ggtgccgtat ccggatccgc tggaaccgcg tgaattcggg 
       61 atccgtatgt cgtctgggga cccaaggtct ggtagacagg acggggcccc gcgtgcggcc 
      121 gcagcgctct gtggcctgta ccacgaggcc ggccagcaac tacagcgcct gaaggatcag 
      181 ctggccgcgc gtgacgccct catcgcgagc ctccgcaccc gcctcgcggc tctggaaggg 
      241 cacacggcgc cgtcactcgt ggacgcactt ctggatcagg tggagcgctt ccgtgagcag 
      301 ctgcgacgac aggaggaagg cgcttcggag acccagctgc ggcaggaagt tgaaagactt 
      361 acggagcgtc tagaggaaaa agagagggag atgcaacagc tgatgagcca gcctcagcat 
      421 gagcaagaga aggaggtagt cttgcttcgg cgaagtgtgg cagagaagga gaaagccagg 
      481 gccgccagtg atgttctgtg ccgctccttg gctgatgaga cccaccaact gcgcaggaca 
      541 ttggcagcca ctgcccacat gtgccaacat ctggccaaat gtctggatga acgacagtgt 
      601 gcacagggag acgctgggga gaaaagccct gctgagctag agcaaacaag cagcgatgct 
      661 tctggccaga gtgttattaa gaagttacag gaagaaaatc gactgttaaa acagaaggtg 
      721 actcatgtag aagacctcaa tgctaagtgg cagcgttatg atgcaagtag ggacgaatat 
      781 gtgaaggggt tgcatgccca gctaaagagg cggcaggtcc ctctggagcc tgagctgatg 
      841 aagaaggaga tttcccgact taacagacag ttggaggaga aaataagtga ctgtgcggaa 
      901 gcaaaccagg agctgacagc catgaggatg tcccgggaca ctgcgctgga gcgagtgcag 
      961 atgctagaac agcagattct tgcttacaag gatgacttca aatcagaaag ggcagatcgg 
     1021 gaacgagcgc acagtaggat tcaagagctg gaggaaaaga tcatgtcctt gatgtaccaa 
     1081 gtgtcccaga gacaggactc ccgggagcca ggaccctgtc ggattcatac ggggaacaaa 
     1141 actgccaagt acttagagat ggatgcactg gagcatgtga cccctggcgg ctggaggcct 
     1201 gagtctaggt cccaacagat ggaaccttct gcagagggtg ggcatgtgtg cacagcccag 
     1261 agaggtcagg gtgaccttca gtgccctcat tgcctgcggt gcttcagtga tgagcaaggc 
     1321 gaggcattcc tcaggcacct gtctgagtgc tgccaatgaa gatctttttc cctctgccaa 
     1381 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt 
     1441 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg 
     1501 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc 
     1561 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca 
     1621 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt 
     1681 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt 
     1741 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt 
     1801 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg 
     1861 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 
     1921 aagcctgggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 
     1981 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata 
     2041 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 
     2101 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 
     2161 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact 
     2221 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 
     2281 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 
     2341 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 
     2401 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
     2461 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag ggataacgca 
     2521 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 
     2581 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 
     2641 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 
     2701 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 
     2761 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 
     2821 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 
     2881 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 
     2941 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 
     3001 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 
     3061 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 
     3121 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 
     3181 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     3241 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 
     3301 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 
     3361 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 
     3421 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 
     3481 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 
     3541 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 
     3601 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 
     3661 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 
     3721 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 
     3781 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 
     3841 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 
     3901 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 
     3961 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 
     4021 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 
     4081 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 
     4141 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 
     4201 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 
     4261 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
     4321 ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat 
     4381 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 
     4441 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 
     4501 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 
     4561 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 
     4621 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 
     4681 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac 
     4741 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 
     4801 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcccc ccccaggcgg 
     4861 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca 
     4921 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 
     4981 aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc 
     5041 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag 
     5101 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt 
     5161 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg 
     5221 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc 
     5281 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt 
     5341 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa 
     5401 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt 
     5461 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg 
     5521 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca 
     5581 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaaggggc 
     5641 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt 
     5701 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga 
     5761 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg 
     5821 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc 
     5881 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg 
     5941 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg 
     6001 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt 
     6061 ttggcaaaga a 
//
