LOCUS       pCAGGS-E-mABIN-2f1 5321 bp dsDNACircular
DEFINITION  LMBP 4371.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     misc_difference 10..51
                     /label=E-tag
     terminator      611..702
                     /label=ß-globin\polyA
     promoter        complement(1137..1219)
                     /label=lac\promoter
     rep_origin      1361..1436
                     /label=SV40\ori
     terminator      1466..1541
                     /label=SV40\polyA\early
     terminator      complement(1466..1541)
                     /label=SV40\polyA\late
     rep_origin      complement(1656..2057)
                     /label=pMB1\ori
     promoter        3594..3971
                     /label=hCMV\enhancer
     promoter        3977..5318
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
                     /label=AG\promoter
     CDS             complement(2598..3458)
                     /label=AMP
     intron          4346..5270
                     /label=intron\AG\promoter
     CDS             64..606
                     /note="Mouse cDNA encoding the mABIN-2 A20-binding inhibitor of NF-kB activation; fragment containing codons 251 to 430."
                     /label=mABIN-2\(251-430)
ORIGIN
        1 ttcctcgaga tgggtgcgcc ggtgccgtat ccggatccgc tggaaccgcg tgaattcggt 
       61 accatggtcc ctctggagcc tgagctgatg aagaaggaga tttcccgact taacagacag 
      121 ttggaggaga aaataagtga ctgtgcggaa gcaaaccagg agctgacagc catgaggatg 
      181 tcccgggaca ctgcgctgga gcgagtgcag atgctagaac agcagattct tgcttacaag 
      241 gatgacttca aatcagaaag ggcagatcgg gaacgagcgc acagtaggat tcaagagctg 
      301 gaggaaaaga tcatgtcctt gatgtaccaa gtgtcccaga gacaggactc ccgggagcca 
      361 ggaccctgtc ggattcatac ggggaacaaa actgccaagt acttagagat ggatgcactg 
      421 gagcatgtga cccctggcgg ctggaggcct gagtctaggt cccaacagat ggaaccttct 
      481 gcagagggtg ggcatgtgtg cacagcccag agaggtcagg gtgaccttca gtgccctcat 
      541 tgcctgcggt gcttcagtga tgagcaaggc gaggcattcc tcaggcacct gtctgagtgc 
      601 tgccaatgaa gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga 
      661 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt 
      721 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga 
      781 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg 
      841 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag 
      901 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt 
      961 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact 
     1021 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct 
     1081 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 
     1141 acaacatacg agccggaagc ataaagtgta aagcctgggt gcctaatgag tgagctaact 
     1201 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg 
     1261 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc 
     1321 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 
     1381 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 
     1441 ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat 
     1501 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 
     1561 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg 
     1621 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 
     1681 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 
     1741 acggttatcc acagaatcag ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 
     1801 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 
     1861 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 
     1921 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 
     1981 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 
     2041 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 
     2101 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 
     2161 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 
     2221 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 
     2281 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 
     2341 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 
     2401 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 
     2461 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 
     2521 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 
     2581 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 
     2641 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 
     2701 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 
     2761 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 
     2821 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 
     2881 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 
     2941 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 
     3001 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 
     3061 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 
     3121 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 
     3181 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 
     3241 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 
     3301 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 
     3361 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 
     3421 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 
     3481 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 
     3541 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg 
     3601 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat 
     3661 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc 
     3721 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca 
     3781 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta 
     3841 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta 
     3901 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat 
     3961 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc 
     4021 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc 
     4081 gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc 
     4141 gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat 
     4201 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt 
     4261 tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct 
     4321 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg 
     4381 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga 
     4441 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt 
     4501 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg 
     4561 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc 
     4621 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg 
     4681 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc 
     4741 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg 
     4801 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc 
     4861 tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc 
     4921 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac 
     4981 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta 
     5041 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc 
     5101 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg 
     5161 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 
     5221 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 
     5281 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga a 
//
