LOCUS       pCAGGS-E-mABIN-2f2 5213 bp dsDNACircular
DEFINITION  LMBP 4372.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     promoter        3869..5210
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
                     /label=AG\promoter
     promoter        3486..3863
                     /note="human CMV-IE enhancer"
                     /label=hCMV\enhancer
     intron          4238..5162
                     /note="chicken ß-actin/ rabbit ß-globin hybrid intron"
                     /label=intron\AG\promoter
     misc_difference 13..51
                     /note="E-tag"
                     /label=E-tag
     promoter        complement(1029..1111)
                     /note="E. coli lac operon promoter"
                     /label=lac\promoter
     rep_origin      1253..1328
                     /note="bidirectional origin of SV40"
                     /label=SV40\ori
     terminator      1359..1433
                     /note="polyA signal of the SV40 early region"
                     /label=SV40\polyA\early
     terminator      complement(1359..1433)
                     /note="polyA signal of the SV40 late region"
                     /label=SV40\polyA\late
     rep_origin      complement(1548..1949)
                     /note="origin plasmid"
                     /label=pMB1\ori
     CDS             complement(2490..3350)
                     /note="antibiotic resistance gene; coding sequence"
                     /label=AMP
     misc_feature    595..951
                     /note="3' flanking region of the rabbit ß-globin gene"
                     /label=3'FR\of\ß-globin
     terminator      503..594
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
                     /label=ß-globin\polyA
     CDS             64..501
                     /note="mouse ABIN-2 cDNA; codon 286 to 430"
                     /label=mABIN-2\(286-430)
ORIGIN
        1 ttcctcgaga tgggtgcgcc ggtgccgtat ccggatccgc tggaaccgcg tgaattcggt 
       61 accatgagga tgtcccggga cactgcgctg gagcgagtgc agatgctaga acagcagatt 
      121 cttgcttaca aggatgactt caaatcagaa agggcagatc gggaacgagc gcacagtagg 
      181 attcaagagc tggaggaaaa gatcatgtcc ttgatgtacc aagtgtccca gagacaggac 
      241 tcccgggagc caggaccctg tcggattcat acggggaaca aaactgccaa gtacttagag 
      301 atggatgcac tggagcatgt gacccctggc ggctggaggc ctgagtctag gtcccaacag 
      361 atggaacctt ctgcagaggg tgggcatgtg tgcacagccc agagaggtca gggtgacctt 
      421 cagtgccctc attgcctgcg gtgcttcagt gatgagcaag gcgaggcatt cctcaggcac 
      481 ctgtctgagt gctgccaatg aagatctttt tccctctgcc aaaaattatg gggacatcat 
      541 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt 
      601 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa 
      661 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat 
      721 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc 
      781 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt 
      841 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct 
      901 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct 
      961 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 
     1021 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg 
     1081 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 
     1141 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 
     1201 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 
     1261 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 
     1321 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata 
     1381 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 
     1441 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca 
     1501 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 
     1561 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 
     1621 aaaggcggta atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca 
     1681 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 
     1741 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 
     1801 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 
     1861 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 
     1921 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 
     1981 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 
     2041 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 
     2101 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 
     2161 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 
     2221 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 
     2281 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 
     2341 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 
     2401 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 
     2461 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 
     2521 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 
     2581 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 
     2641 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 
     2701 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 
     2761 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 
     2821 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 
     2881 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 
     2941 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 
     3001 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 
     3061 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 
     3121 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 
     3181 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 
     3241 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 
     3301 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 
     3361 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 
     3421 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 
     3481 gggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca 
     3541 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 
     3601 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 
     3661 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 
     3721 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 
     3781 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 
     3841 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc 
     3901 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc 
     3961 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg 
     4021 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa 
     4081 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg 
     4141 ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg 
     4201 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc 
     4261 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg 
     4321 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc 
     4381 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga 
     4441 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc 
     4501 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg 
     4561 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct 
     4621 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg 
     4681 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg 
     4741 ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg 
     4801 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga 
     4861 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc 
     4921 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg 
     4981 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct 
     5041 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc 
     5101 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac 
     5161 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaa 
//
