LOCUS       pCAGGS-E-mABIN-2f3 5068 bp dsDNACircular
DEFINITION  LMBP 4373.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     promoter        3345..3722
                     /label=hCMV\enhancer
     rep_origin      1402..1804
                     /label=pMB1\ori
     promoter        complement(868..951)
                     /label=lac\promoter
     terminator      341..432
                     /label=ß-globin\polyA
     misc_difference 13..51
                     /label=E-tag
     rep_origin      1106..1181
                     /label=SV40\ori
     terminator      1213..1288
                     /label=SV40\polyA\early
     terminator      complement(1213..1288)
                     /label=SV40\polyA\late
     promoter        3734..5065
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
                     /label=AG\promoter
     CDS             complement(2345..3205)
                     /label=AMP
     CDS             64..339
                     /note="Mouse cDNA encoding the mABIN-2 A20-binding inhibitor of NF-kB activation; fragment containing codons 341 to 430."
                     /label=mABIN-2\(341-430)
     intron          4098..5017
                     /label=intron\AG\promoter
ORIGIN
        1 ttcctcgaga tgggtgcgcc ggtgccgtat ccggatccgc tggaaccgcg tgaattcggt 
       61 accatgcaga gacaggactc ccgggagcca ggaccctgtc ggattcatac ggggaacaaa 
      121 actgccaagt acttagagat ggatgcactg gagcatgtga cccctggcgg ctggaggcct 
      181 gagtctaggt cccaacagat ggaaccttct gcagagggtg ggcatgtgtg cacagcccag 
      241 agaggtcagg gtgaccttca gtgccctcat tgcctgcggt gcttcagtga tgagcaaggc 
      301 gaggcattcc tcaggcacct gtctgagtgc tgccaatgaa gatctttttc cctctgccaa 
      361 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt 
      421 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg 
      481 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc 
      541 catatgctgg ctgccatgaa caaaggtggc tataaagagg tcatcagtat atgaaacagc 
      601 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta 
      661 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac 
      721 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat 
      781 gaagatcnnn nctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct 
      841 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 
      901 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 
      961 gctttccagt cgggaaacct gtcgtgccag nnnnnnnnnn nggatccnat gcatctcaat 
     1021 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 
     1081 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 
     1141 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 
     1201 tgcaaaaagc tnaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 
     1261 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 
     1321 atcaatgtat cttatcatgt ctggatccnc tgcattaatg aatcggccaa cgcgcgggga 
     1381 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 
     1441 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 
     1501 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 
     1561 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 
     1621 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 
     1681 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 
     1741 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 
     1801 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
     1861 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 
     1921 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 
     1981 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 
     2041 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 
     2101 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 
     2161 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 
     2221 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 
     2281 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 
     2341 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 
     2401 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 
     2461 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 
     2521 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 
     2581 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 
     2641 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 
     2701 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 
     2761 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 
     2821 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 
     2881 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 
     2941 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 
     3001 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 
     3061 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 
     3121 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 
     3181 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 
     3241 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 
     3301 gggttccgcg cacatttccc cgaaaagtgc cacctgnnnn ngtcgacatt gattattgac 
     3361 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
     3421 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
     3481 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
     3541 atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
     3601 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
     3661 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
     3721 catggnnnnn gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc 
     3781 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg 
     3841 gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc 
     3901 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga 
     3961 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgt 
     4021 tgccttcgcc ccgtgccccg ctccgcgccg cctcgcgccg cccgccccgg ctctgactga 
     4081 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc 
     4141 gcttggttta atgacggctc gtttcttttc tgtggctgcg tgaaagcctt aaagggctcc 
     4201 gggagggccc tttgtgcggg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg 
     4261 gggagcgccg cgtgcggccc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg 
     4321 ggctttgtgc gctccgcgtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg 
     4381 cgggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca 
     4441 gggggtgtgg gcgcggcggt cgggctgtaa cccccccctg cacccccctc cccgagttgc 
     4501 tgagcacggc ccggcttcgg gtgcggggct ccgtgcgggg cgtggcgcgg ggctcgccgt 
     4561 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg 
     4621 ggagggctcg ggggaggggc gcggcggccc cggagcgccg gcggctgtcg aggcgcggcg 
     4681 agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc 
     4741 caaatctggc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggc 
     4801 gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg 
     4861 ccgccgtccc cttctccatc tccagcctcg gggctgccgc agggggacgg ctgccttcgg 
     4921 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcgggnt ctagancctc 
     4981 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat 
     5041 tgtgctgtct catcattttg gcaaagaa 
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