LOCUS       pCAGGS-E-mABIN-2f4 5531 bp dsDNACircular
DEFINITION  LMBP 4374.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     misc_difference 13..51
                     /label=E-tag
     terminator      821..912
                     /label=ß-globin\polyA
     promoter        complement(1347..1429)
                     /label=lac\promoter
     terminator      1676..1751
                     /label=SV40\polyA\early
     terminator      complement(1676..1751)
                     /label=SV40\polyA\late
     rep_origin      complement(1866..2267)
                     /label=pMB1\ori
     promoter        3804..4181
                     /label=hCMV\enhancer
     rep_origin      1571..1646
                     /label=SV40\ori
     intron          4556..5480
                     /label=intron\AG\promoter
     promoter        4187..5528
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
                     /label=AG\promoter
     CDS             complement(2808..3668)
                     /label=AMP
     CDS             67..819
                     /note="Mouse cDNA encoding the mABIN-2 A20-binding inhibitor of NF-kB activation; fragment containing codons 1 to 250."
                     /label=mABIN-2\(1-250)
ORIGIN
        1 ttcctcgaga tgggtgcgcc ggtgccgtat ccggatccgc tggaaccgcg tgaattcggg 
       61 atccgtatgt cgtctgggga cccaaggtct ggtagacagg acggggcccc gcgtgcggcc 
      121 gcagcgctct gtggcctgta ccacgaggcc ggccagcaac tacagcgcct gaaggatcag 
      181 ctggccgcgc gtgacgccct catcgcgagc ctccgcaccc gcctcgcggc tctggaaggg 
      241 cacacggcgc cgtcactcgt ggacgcactt ctggatcagg tggagcgctt ccgtgagcag 
      301 ctgcgacgac aggaggaagg cgcttcggag acccagctgc ggcaggaagt tgaaagactt 
      361 acggagcgtc tagaggaaaa agagagggag atgcaacagc tgatgagcca gcctcagcat 
      421 gagcaagaga aggaggtagt cttgcttcgg cgaagtgtgg cagagaagga gaaagccagg 
      481 gccgccagtg atgttctgtg ccgctccttg gctgatgaga cccaccaact gcgcaggaca 
      541 ttggcagcca ctgcccacat gtgccaacat ctggccaaat gtctggatga acgacagtgt 
      601 gcacagggag acgctgggga gaaaagccct gctgagctag agcaaacaag cagcgatgct 
      661 tctggccaga gtgttattaa gaagttacag gaagaaaatc gactgttaaa acagaaggtg 
      721 actcatgtag aagacctcaa tgctaagtgg cagcgttatg atgcaagtag ggacgaatat 
      781 gtgaaggggt tgcatgccca gctaaagagg cggcagtgaa gatctttttc cctctgccaa 
      841 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt 
      901 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg 
      961 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc 
     1021 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca 
     1081 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt 
     1141 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt 
     1201 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt 
     1261 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg 
     1321 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 
     1381 aagcctgggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 
     1441 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata 
     1501 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 
     1561 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 
     1621 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact 
     1681 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 
     1741 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 
     1801 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 
     1861 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
     1921 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag ggataacgca 
     1981 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 
     2041 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 
     2101 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 
     2161 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 
     2221 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 
     2281 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 
     2341 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 
     2401 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 
     2461 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 
     2521 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 
     2581 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 
     2641 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     2701 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 
     2761 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 
     2821 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 
     2881 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 
     2941 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 
     3001 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 
     3061 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 
     3121 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 
     3181 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 
     3241 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 
     3301 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 
     3361 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 
     3421 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 
     3481 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 
     3541 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 
     3601 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 
     3661 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 
     3721 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
     3781 ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat 
     3841 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 
     3901 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 
     3961 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 
     4021 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 
     4081 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 
     4141 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac 
     4201 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 
     4261 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcccc ccccaggcgg 
     4321 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca 
     4381 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 
     4441 aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc 
     4501 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag 
     4561 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt 
     4621 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg 
     4681 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc 
     4741 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt 
     4801 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa 
     4861 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt 
     4921 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg 
     4981 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca 
     5041 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaaggggc 
     5101 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt 
     5161 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga 
     5221 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg 
     5281 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc 
     5341 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg 
     5401 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg 
     5461 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt 
     5521 ttggcaaaga a 
//
