LOCUS       pCAGGS-E-mABIN-2d2 6014 bp dsDNACircular
DEFINITION  LMBP 4378.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     misc_difference 13..51
                     /label=E-tag
     terminator      1304..1395
                     /label=ß-globin\polyA
     promoter        complement(1830..1912)
                     /label=lac\promoter
     terminator      2159..2234
                     /label=SV40\polyA\early
     terminator      complement(2159..2234)
                     /label=SV40\polyA\late
     rep_origin      complement(2349..2750)
                     /label=pMB1\ori
     promoter        4287..4664
                     /label=hCMV\enhancer
     rep_origin      2054..2129
                     /label=SV40\ori
     intron          5039..5963
                     /label=intron\AG\promoter
     promoter        4670..6011
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
                     /label=AG\promoter
     CDS             complement(3291..4151)
                     /label=AMP
     CDS             67..1302
                     /note="Mouse cDNA encoding the mABIN-2 A20-binding inhibitor of NF-kB activation with an internal deletion of codons 256 to 274"
                     /label=mABIN-2\(d256-274)
ORIGIN
        1 ttcctcgaga tgggtgcgcc ggtgccgtat ccggatccgc tggaaccgcg tgaattcggg 
       61 atccgtatgt cgtctgggga cccaaggtct ggtagacagg acggggcccc gcgtgcggcc 
      121 gcagcgctct gtggcctgta ccacgaggcc ggccagcaac tacagcgcct gaaggatcag 
      181 ctggccgcgc gtgacgccct catcgcgagc ctccgcaccc gcctcgcggc tctggaaggg 
      241 cacacggcgc cgtcactcgt ggacgcactt ctggatcagg tggagcgctt ccgtgagcag 
      301 ctgcgacgac aggaggaagg cgcttcggag acccagctgc ggcaggaagt tgaaagactt 
      361 acggagcgtc tagaggaaaa agagagggag atgcaacagc tgatgagcca gcctcagcat 
      421 gagcaagaga aggaggtagt cttgcttcgg cgaagtgtgg cagagaagga gaaagccagg 
      481 gccgccagtg atgttctgtg ccgctccttg gctgatgaga cccaccaact gcgcaggaca 
      541 ttggcagcca ctgcccacat gtgccaacat ctggccaaat gtctggatga acgacagtgt 
      601 gcacagggag acgctgggga gaaaagccct gctgagctag agcaaacaag cagcgatgct 
      661 tctggccaga gtgttattaa gaagttacag gaagaaaatc gactgttaaa acagaaggtg 
      721 actcatgtag aagacctcaa tgctaagtgg cagcgttatg atgcaagtag ggacgaatat 
      781 gtgaaggggt tgcatgccca gctaaagagg cggcaggtcc ctctggagcc tgactgtgcg 
      841 gaagcaaacc aggagctgac agccatgagg atgtcccggg acactgcgct ggagcgagtg 
      901 cagatgctag aacagcagat tcttgcttac aaggatgact tcaaatcaga aagggcagat 
      961 cgggaacgag cgcacagtag gattcaagag ctggaggaaa agatcatgtc cttgatgtac 
     1021 caagtgtccc agagacagga ctcccgggag ccaggaccct gtcggattca tacggggaac 
     1081 aaaactgcca agtacttaga gatggatgca ctggagcatg tgacccctgg cggctggagg 
     1141 cctgagtcta ggtcccaaca gatggaacct tctgcagagg gtgggcatgt gtgcacagcc 
     1201 cagagaggtc agggtgacct tcagtgccct cattgcctgc ggtgcttcag tgatgagcaa 
     1261 ggcgaggcat tcctcaggca cctgtctgag tgctgccaat gaagatcttt ttccctctgc 
     1321 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa 
     1381 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata 
     1441 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata 
     1501 tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa 
     1561 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt 
     1621 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt 
     1681 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct 
     1741 cttatggaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc 
     1801 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 
     1861 gtaaagcctg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 
     1921 cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc 
     1981 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct 
     2041 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 
     2101 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta 
     2161 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 
     2221 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 
     2281 atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 
     2341 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 
     2401 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat cagggataac 
     2461 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 
     2521 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 
     2581 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 
     2641 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 
     2701 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 
     2761 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 
     2821 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 
     2881 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 
     2941 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 
     3001 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 
     3061 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 
     3121 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 
     3181 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 
     3241 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 
     3301 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 
     3361 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 
     3421 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 
     3481 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 
     3541 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 
     3601 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 
     3661 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 
     3721 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 
     3781 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 
     3841 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 
     3901 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 
     3961 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 
     4021 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 
     4081 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 
     4141 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 
     4201 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 
     4261 tttccccgaa aagtgccacc tgggtcgaca ttgattattg actagttatt aatagtaatc 
     4321 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt 
     4381 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 
     4441 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 
     4501 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 
     4561 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 
     4621 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc 
     4681 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt 
     4741 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc cccccccagg 
     4801 cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg aaaggtgcgg cggcagccaa 
     4861 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta 
     4921 taaaaagcga agcgcgcggc gggcgggagt cgttgcgcgc tgccttcccc ccgtgccccg 
     4981 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt 
     5041 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct 
     5101 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg 
     5161 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc 
     5221 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag 
     5281 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg 
     5341 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc 
     5401 ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc 
     5461 gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg 
     5521 gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaagg 
     5581 ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc 
     5641 ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc 
     5701 cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg 
     5761 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc 
     5821 tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca 
     5881 gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc 
     5941 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc 
     6001 attttggcaa agaa 
//
