LOCUS       pCAGGS-E-mABIN-2d3 5876 bp dsDNACircular
DEFINITION  LMBP 4379.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     misc_difference 13..51
                     /label=E-tag
     terminator      1166..1257
                     /label=ß-globin\polyA
     promoter        complement(1692..1774)
                     /label=lac\promoter
     terminator      2021..2096
                     /label=SV40\polyA\early
     terminator      complement(2021..2096)
                     /label=SV40\polyA\late
     rep_origin      complement(2211..2612)
                     /label=pMB1\ori
     promoter        4149..4526
                     /label=hCMV\enhancer
     rep_origin      1916..1991
                     /label=SV40\ori
     intron          4901..5825
                     /label=intron\AG\promoter
     promoter        4532..5873
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
                     /label=AG\promoter
     CDS             complement(3153..4013)
                     /label=AMP
     CDS             67..1164
                     /note="Mouse cDNA encoding the mABIN-2 A20-binding inhibitor of NF-kB activation with an internal deletion of codons 256 to 320"
                     /label=mABIN-2\(d256-320)
ORIGIN
        1 ttcctcgaga tgggtgcgcc ggtgccgtat ccggatccgc tggaaccgcg tgaattcggg 
       61 atccgtatgt cgtctgggga cccaaggtct ggtagacagg acggggcccc gcgtgcggcc 
      121 gcagcgctct gtggcctgta ccacgaggcc ggccagcaac tacagcgcct gaaggatcag 
      181 ctggccgcgc gtgacgccct catcgcgagc ctccgcaccc gcctcgcggc tctggaaggg 
      241 cacacggcgc cgtcactcgt ggacgcactt ctggatcagg tggagcgctt ccgtgagcag 
      301 ctgcgacgac aggaggaagg cgcttcggag acccagctgc ggcaggaagt tgaaagactt 
      361 acggagcgtc tagaggaaaa agagagggag atgcaacagc tgatgagcca gcctcagcat 
      421 gagcaagaga aggaggtagt cttgcttcgg cgaagtgtgg cagagaagga gaaagccagg 
      481 gccgccagtg atgttctgtg ccgctccttg gctgatgaga cccaccaact gcgcaggaca 
      541 ttggcagcca ctgcccacat gtgccaacat ctggccaaat gtctggatga acgacagtgt 
      601 gcacagggag acgctgggga gaaaagccct gctgagctag agcaaacaag cagcgatgct 
      661 tctggccaga gtgttattaa gaagttacag gaagaaaatc gactgttaaa acagaaggtg 
      721 actcatgtag aagacctcaa tgctaagtgg cagcgttatg atgcaagtag ggacgaatat 
      781 gtgaaggggt tgcatgccca gctaaagagg cggcaggtcc ctctggagcc tgcgcacagt 
      841 aggattcaag agctggagga aaagatcatg tccttgatgt accaagtgtc ccagagacag 
      901 gactcccggg agccaggacc ctgtcggatt catacgggga acaaaactgc caagtactta 
      961 gagatggatg cactggagca tgtgacccct ggcggctgga ggcctgagtc taggtcccaa 
     1021 cagatggaac cttctgcaga gggtgggcat gtgtgcacag cccagagagg tcagggtgac 
     1081 cttcagtgcc ctcattgcct gcggtgcttc agtgatgagc aaggcgaggc attcctcagg 
     1141 cacctgtctg agtgctgcca atgaagatct ttttccctct gccaaaaatt atggggacat 
     1201 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat 
     1261 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt 
     1321 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc 
     1381 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca 
     1441 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt 
     1501 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt 
     1561 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga 
     1621 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 
     1681 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tgggtgccta 
     1741 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 
     1801 cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact 
     1861 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 
     1921 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 
     1981 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt 
     2041 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 
     2101 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct 
     2161 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 
     2221 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 
     2281 ctcaaaggcg gtaatacggt tatccacaga atcagggata acgcaggaaa gaacatgtga 
     2341 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 
     2401 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 
     2461 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 
     2521 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 
     2581 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 
     2641 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 
     2701 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 
     2761 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 
     2821 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 
     2881 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 
     2941 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 
     3001 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 
     3061 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 
     3121 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 
     3181 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 
     3241 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 
     3301 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 
     3361 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 
     3421 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 
     3481 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 
     3541 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 
     3601 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 
     3661 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 
     3721 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 
     3781 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 
     3841 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 
     3901 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 
     3961 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 
     4021 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 
     4081 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 
     4141 cctgggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt 
     4201 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 
     4261 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 
     4321 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 
     4381 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 
     4441 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 
     4501 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct 
     4561 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg 
     4621 tgcagcgatg ggggcggggg gggggggggg gcccccccca ggcggggcgg ggcggggcga 
     4681 ggggcggggc ggggcgaggc ggaaaggtgc ggcggcagcc aatcagagcg gcgcgctccg 
     4741 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg 
     4801 gcgggcggga gtcgttgcgc gctgccttcc ccccgtgccc cgctccgccg ccgcctcgcg 
     4861 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc 
     4921 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct 
     4981 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg 
     5041 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg 
     5101 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc 
     5161 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg 
     5221 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc 
     5281 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac 
     5341 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg 
     5401 cggggcgggg ccgcctcggg ccggggaggg ctcgggggaa ggggcgcggc ggcccccgga 
     5461 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg 
     5521 agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc 
     5581 gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg 
     5641 gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg 
     5701 gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct 
     5761 ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc 
     5821 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaa 
//
