LOCUS       pCAGGShCuZnSOD 5416 bp dsDNA  Circular
DEFINITION  LMBP 4428.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     CDS             75..539
                     /note="superoxide dismutase (aa 1-154)"
                     /label=hCuZnSOD
     terminator      706..797
                     /label=ß-globin\polyA
     promoter        complement(1232..1314)
                     /label=lac\promoter
     rep_origin      1456..1531
                     /label=SV40\ori
     terminator      1561..1636
                     /label=SV40\polyA\early
     terminator      complement(1561..1636)
                     /label=SV40\polyA\late
     rep_origin      complement(1751..2152)
                     /label=pMB1\ori
     CDS             complement(2693..3553)
                     /label=AMP
     promoter        3689..4066
                     /label=hCMV\enhancer
     promoter        4072..5413
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
                     /label=AG\promoter
     intron          4441..5365
                     /label=intron\AG\promoter
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg 
       61 ggatccccgg ggggatggcg acgaaggccg tgtgcgtgct gaagggcgac ggcccagtgc 
      121 agggcatcat caatttcgag cagaaggaaa gtaatggacc agtgaaggtg tggggaagca 
      181 ttaaaggact gactgaaggc ctgcatggat tccatgttca tgagtttgga gataatacag 
      241 caggctgtac cagtgcaggt cctcacttta atcctctatc cagaaaacac ggtgggccaa 
      301 aggatgaaga gaggcatgtt ggagacttgg gcaatgtgac tgctgacaaa gatggtgtgg 
      361 ccgatgtgtc tattgaagat tctgtgatct cactctcagg agaccattgc atcattggcc 
      421 gcacactggt ggtccatgaa aaagcagatg acttgggcaa aggtggaaat gaagaaagta 
      481 caaagacagg aaacgctgga agtcgtttgg cttgtggtgt aattgggatc gcccaataaa 
      541 cattcccttg gatgtagtct gaggcccctt aactcatctg ttatcctgct agctgtagaa 
      601 atgtatcctg ataaacatta aacactgtaa tcttaaaaaa aaaacccccc ccccgggcga 
      661 gctcgaattc gggatcccaa tgccctggct cacaaatacc actgagatct ttttccctct 
      721 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg 
      781 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca 
      841 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca 
      901 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg 
      961 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt 
     1021 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat 
     1081 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt 
     1141 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt 
     1201 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 
     1261 gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 
     1321 cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa 
     1381 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 
     1441 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 
     1501 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 
     1561 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 
     1621 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 
     1681 ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg 
     1741 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 
     1801 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcagggata 
     1861 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 
     1921 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 
     1981 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 
     2041 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 
     2101 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 
     2161 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 
     2221 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 
     2281 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 
     2341 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 
     2401 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 
     2461 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 
     2521 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 
     2581 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 
     2641 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 
     2701 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 
     2761 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 
     2821 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 
     2881 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 
     2941 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 
     3001 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 
     3061 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 
     3121 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 
     3181 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 
     3241 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 
     3301 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 
     3361 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 
     3421 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 
     3481 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 
     3541 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 
     3601 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 
     3661 catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta ttaatagtaa 
     3721 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg 
     3781 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg 
     3841 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta 
     3901 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt 
     3961 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac 
     4021 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc 
     4081 cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta 
     4141 tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg gcccccccca 
     4201 ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggaaaggtgc ggcggcagcc 
     4261 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc 
     4321 tataaaaagc gaagcgcgcg gcgggcggga gtcgttgcgc gctgccttcc ccccgtgccc 
     4381 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag 
     4441 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg 
     4501 cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc 
     4561 ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc 
     4621 tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc 
     4681 agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag 
     4741 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg 
     4801 tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct 
     4861 tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg 
     4921 cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggaa 
     4981 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg 
     5041 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga 
     5101 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg 
     5161 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct 
     5221 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg 
     5281 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt 
     5341 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca 
     5401 tcattttggc aaagaa 
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