LOCUS       pCAGGSbGPx              5644 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 4429.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pCAGGSbGPx|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGSbGPx" 0 5644 
COMMENT       (CDBMol 0 0 1 1 1 0 0 262162 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 5644 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "bGPx" 4 0 0 101 718 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "101..718") 
COMMENT        #4=(CFSignal (CObList) "3UTR" 21 0 0 719 897 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "719..897") 
COMMENT        #5=(CFSignal (CObList) "polyA" 43 0 0 934 1025 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "934..1025") 
COMMENT        #6=(CFSignal (CObList) "3FR" 21 0 0 1026 1382 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1026..1382") 
COMMENT        #7=(CFSignal (CObList) "lac" 30 0 1 1460 1542 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1460..1542)") 
COMMENT        #8=(CFSignal (CObList) "SVORI" 33 0 0 1684 1759 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1684..1759") 
COMMENT        #9=(CFSignal (CObList) "polyA" 43 0 0 1790 1864 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1790..1864") 
COMMENT        #10=(CFSignal (CObList) "polyA" 43 0 1 1790 1864 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1790..1864)") 
COMMENT        #11=(CFSignal (CObList) "ORI" 33 0 1 1979 2380 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1979..2380)") 
COMMENT        #12=(CFSignal (CObList) "AMP" 4 0 1 2921 3781 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2921..3781)") 
COMMENT        #13=(CFSignal (CObList) "hCMV" 21 0 0 3917 4294 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3917..4294") 
COMMENT        #14=(CFSignal (CObList) "AG" 30 0 0 4300 5641 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4300..5641") 
COMMENT        #15=(CFSignal (CObList) "INT" 15 0 0 4669 5593 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4669..5593")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
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COMMENT             (CObjectList 
COMMENT              #17=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pCAGGSbGPx" 2) 5 
COMMENT                   "" 0 4) 
COMMENT              #18=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pCAGGSbGPx'" 1) 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5644 bp" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                    (CObjectList 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #28=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #29=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #30=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #31=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #32=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 101 (3 #3# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #33=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 101   End: 718 " 1) 
COMMENT                                   #34=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "bovine selenium glutathione peroxidase coding seq." 
COMMENT                                        1) 
COMMENT                                   #35=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #36=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   101..718" 1))) "bGPx") 
COMMENT                            #37=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2921 (3 #12# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #38=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2921  End: 3781 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #39=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #40=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #41=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2921..3781)" 1))) "AMP"))) 
COMMENT                          "CDS (2 total)") 
COMMENT                     #42=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 15 (7 15 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #43=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4669 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4669  End: 5593" 1) 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chicken \347-actin/rabbit \347-globin hybrid intron" 
COMMENT                                        1) 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4669..5593" 1))) "INT"))) 
COMMENT                          "Intron (1 total)") 
COMMENT                     #48=(CFolderPar 
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COMMENT                            #49=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 719   End: 897 " 1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "3' UTR of the bovine GPx gene" 1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   719..897" 1))) "3UTR") 
COMMENT                            #54=(CFolderPar 
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COMMENT                                  (CParagraph 1026 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1026  End: 1382" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "3' flanking region of the rabbit \347-globin gene" 
COMMENT                                        1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1026..1382" 1))) "3FR") 
COMMENT                            #59=(CFolderPar 
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COMMENT                                  (CParagraph 3917 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3917  End: 4294" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "human CMV-IE enhancer" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3917..4294" 1))) "hCMV"))) 
COMMENT                          "Misc. Feature (3 total)") 
COMMENT                     #64=(CFolderPar 
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COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1460  End: 1542 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "E. coli lac operon promoter" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #69=(CLinePar 
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COMMENT                                        "   complement(1460..1542)" 1))) "lac") 
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COMMENT                                        "Start: 4300  End: 5641" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chicken \347-actin/rabbit \347-globin hybrid promoter" 
COMMENT                                        1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4300..5641" 1))) "AG"))) 
COMMENT                          "Promoter Prokaryotic (2 total)") 
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COMMENT                                   #77=(CLinePar 
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COMMENT                                        "Start: 1684  End: 1759" 1) 
COMMENT                                   #78=(CLinePar 
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COMMENT                                        "bidirectional origin of SV40" 1) 
COMMENT                                   #79=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #80=(CLinePar 
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COMMENT                                        "   1684..1759" 1))) "SVORI") 
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COMMENT                                   #82=(CLinePar 
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COMMENT                                        "Start: 1979  End: 2380 (Complementary)" 
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COMMENT                                   #83=(CLinePar 
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COMMENT                                        "origin plasmid" 1) 
COMMENT                                   #84=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        "   complement(1979..2380)" 1))) "ORI"))) 
COMMENT                          "Replication Origin (2 total)") 
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COMMENT                                   #88=(CLinePar 
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COMMENT                                        "Start: 934   End: 1025" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "3' UTR of the rabbit \347-globin gene, incl. polyA" 
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COMMENT                                   #90=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #91=(CLinePar 
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COMMENT                                        "   934..1025" 1))) "polyA") 
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COMMENT                                        "Start: 1790  End: 1864" 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "polyA signal of the SV40 early region" 
COMMENT                                        1) 
COMMENT                                   #95=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #96=(CLinePar 
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COMMENT                                        "   1790..1864" 1))) "polyA") 
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COMMENT                                        "polyA signal of the SV40 late region" 1) 
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COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #101=(CLinePar 
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COMMENT                                         "   complement(1790..1864)" 1))) 
COMMENT                                 "polyA"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map")))) 
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COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #108=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
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FEATURES             Location/Qualifiers
     CDS             101..718
                     /vntifkey="4"
                     /label=bGPx
                     /note="bovine selenium glutathione peroxidase coding seq."
     misc_feature    719..897
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the bovine GPx gene"
     terminator      934..1025
                     /vntifkey="43"
                     /label=polyA
                     /note="3' UTR of the rabbit ç-globin gene, incl. polyA"
     misc_feature    1026..1382
                     /vntifkey="21"
                     /label=3FR
                     /note="3' flanking region of the rabbit ç-globin gene"
     promoter        complement(1460..1542)
                     /vntifkey="30"
                     /label=lac
                     /note="E. coli lac operon promoter"
     rep_origin      1684..1759
                     /vntifkey="33"
                     /label=SVORI
                     /note="bidirectional origin of SV40"
     terminator      1790..1864
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 early region"
     terminator      complement(1790..1864)
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(1979..2380)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(2921..3781)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
     misc_feature    3917..4294
                     /vntifkey="21"
                     /label=hCMV
                     /note="human CMV-IE enhancer"
     promoter        4300..5641
                     /vntifkey="30"
                     /label=AG
                     /note="chicken ç-actin/rabbit ç-globin hybrid promoter"
     intron          4669..5593
                     /vntifkey="15"
                     /label=INT
                     /note="chicken ç-actin/rabbit ç-globin hybrid intron"
BASE COUNT     1149 a      1556 c      1578 g      1356 t         5 others
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg 
       61 ggatccnnng cgctctgctc tggattcgga aacggatacc atgtgcgccg ctcagcgctc 
      121 ggcggccgcc ctggcggcgg cagccccgcg cacagtgtac gccttctccg cgcgccctct 
      181 ggccggcggg gagcccttca acctgtcctc cctgcggggc aaggtgctgc tcattgagaa 
      241 cgtagcatcg ctctgaggca caacggtgcg ggactacacc cagatgaatg acctgcagcg 
      301 gcgccttgga ccccggggcc tggtcgtgct cggcttcccc tgcaaccagt ttgggcatca 
      361 ggaaaacgcc aagaacgagg agatcctgaa ttgcctgaag tacgtccgac caggcggcgg 
      421 gttcgagccc aactttatgc tcttcgaaaa gtgcgaggtg aatggcgaga aggcgcatcc 
      481 gctcttcgcc ttccttcggg aggttctgcc cacgccaagt gacgacgcca ctgctctcat 
      541 gaccgaccct aagttcatca cctggtcccc ggtgtgccgc aacgacgtct cctggaactt 
      601 cgagaagttc ctggtgggcc cagacggtgt gcccgtgcgc aggtacagcc gccgctttct 
      661 gaccatcgac atcgagcctg acattgaaac cctgctgtcc cagggggcct ctgcctaggg 
      721 tgcccctccc acccttgctg cttggcggtc agcgctgctc tccagggatt ttgcccatga 
      781 aggtgttccc tctaaaccta cgtggaggaa tgcctgatgt ccaggaaaat cccctgaggt 
      841 gggcgctggt cctatccatc ccagcctccc ctttcccaga ccacagctcc tcatgacnng 
      901 gatcccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat 
      961 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc 
     1021 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca 
     1081 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg 
     1141 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct 
     1201 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt 
     1261 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc 
     1321 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga 
     1381 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 
     1441 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 
     1501 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 
     1561 tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc 
     1621 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 
     1681 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 
     1741 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat 
     1801 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 
     1861 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 
     1921 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 
     1981 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 
     2041 tcagctcact caaaggcggt aatacggtta tccacagaat cagggataac gcaggaaaga 
     2101 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 
     2161 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 
     2221 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 
     2281 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 
     2341 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 
     2401 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 
     2461 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 
     2521 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 
     2581 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 
     2641 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 
     2701 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 
     2761 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 
     2821 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 
     2881 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 
     2941 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 
     3001 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 
     3061 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 
     3121 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 
     3181 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 
     3241 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 
     3301 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 
     3361 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 
     3421 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 
     3481 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 
     3541 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 
     3601 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 
     3661 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 
     3721 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 
     3781 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 
     3841 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 
     3901 aagtgccacc tgggtcgaca ttgattattg actagttatt aatagtaatc aattacgggg 
     3961 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 
     4021 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 
     4081 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 
     4141 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 
     4201 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 
     4261 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc 
     4321 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa 
     4381 ttattttgtg cagcgatggg ggcggggggg gggggggggc cccccccagg cggggcgggg 
     4441 cggggcgagg ggcggggcgg ggcgaggcgg aaaggtgcgg cggcagccaa tcagagcggc 
     4501 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga 
     4561 agcgcgcggc gggcgggagt cgttgcgcgc tgccttcccc ccgtgccccg ctccgccgcc 
     4621 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg 
     4681 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt 
     4741 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc 
     4801 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc 
     4861 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag 
     4921 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc 
     4981 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg 
     5041 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg 
     5101 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg 
     5161 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaagg ggcgcggcgg 
     5221 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta 
     5281 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg 
     5341 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa 
     5401 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca 
     5461 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt 
     5521 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt 
     5581 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa 
     5641 agaa 
//
