LOCUS       pCAGGSpPHGPx            5620 bp    DNA     circular     15-APR-2014
DEFINITION  LMBP 4431.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4431
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 15-APR-2014
            
COMMENT     VNTDATE|406113983|
COMMENT     VNTNAME|pCAGGSpPHGPx|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGSpPHGPx" 0 5620 
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COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "NcoI" "PvuII" "XhoI" "SphI") 
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COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "PvuII" "cagctg" 4 0 1 248 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 3 5 0 73 0 694 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "SphI" "gcatgc" 6 0 1 470 0 0 "")) 
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COMMENT            1002 1358 0 (CStringList) (CStringList) 1 1 1 1 "183..539") 
COMMENT        #6=(CFSignal (CObList) "lac promoter" 30 0 1 1436 1518 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(617..699)") 
COMMENT        #7=(CFSignal (CObList) "SV40 ori" 33 0 0 1660 1735 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "841..916") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1766 1840 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "947..1021") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1766 1840 0 
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COMMENT        #10=(CFSignal (CObList) "pMB1 ori" 33 0 1 1955 2356 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2897 3757 0 
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COMMENT        #13=(CFSignal (CObList) "AG promoter" 29 0 0 4276 5617 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "intron AG promoter" 15 0 0 4645 5569 0 
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COMMENT        #15=(CFSignal (CObList) "pPHGPx" 4 0 0 83 595 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pCAGGSpPHGPx'" 1) 
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COMMENT                          "Created: 03/28/06 09:26" 1) 
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COMMENT                          "Last Modified: 04/15/14 03:25" 1) 
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FEATURES             Location/Qualifiers
     terminator      910..1001
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_feature    1002..1358
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     promoter        complement(1436..1518)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      1660..1735
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      1766..1840
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(1766..1840)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(1955..2356)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(2897..3757)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        3893..4270
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     promoter        4276..5617
                     /vntifkey="29"
                     /label=AG\promoter
     intron          4645..5569
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             83..595
                     /vntifkey="4"
                     /label=pPHGPx
     misc_feature    596..814
                     /vntifkey="21"
                     /label=3'\UTR\of\pPHGPx
BASE COUNT     1205 a      1497 c      1567 g      1351 t 
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg 
       61 gcgggccccc cctcgagaac atatgtgcgc gtcccgcgac gactggcgat gtgctcgctc 
      121 catgcacgaa ttctcagcca aggacatcga cgggcacatg gtgaacctgg acaagtaccg 
      181 gggctacgtg tgcatcgtca ccaatgtggc ctctcaatga ggcaagacgg aggtaaacta 
      241 cactcagctg gtcgacctgc acgcccgata tgctgagtgt ggtttacgga ttctggcctt 
      301 cccttgcaac cagtttggga ggcaggagcc agggagtgat gctgagatca aagaatttgc 
      361 tgctggctac aacgtcaaat ttgatatgtt cagcaagatc tgtgtgaatg gggacgatgc 
      421 ccaccctctg tggaagtgga tgaaagtcca gcccaagggg aggggcatgc tgggaaatgc 
      481 tatcaaatgg aactttacca agttcctcat tgataagaac ggctgtgtgg tgaagcggta 
      541 cggtcccatg gaagagcccc aggtcataga gaaggacctg ccgtgctacc tctagctcaa 
      601 caagtgtgtg ctgagcctgc tgcctgtgcc ctcagagcct tccacctggc acccatgaca 
      661 gtctgcctaa aaaccagccc tggtggggca gactcgagaa cctggcgtgc aaccccgcca 
      721 gaggaaggtc ccttggccca gcttgcagct cgcaccccac cccaggctgc cttgtgggaa 
      781 taaaccatac aaattggcta aaaaaaaaaa aaaaacccga attcctgcag cccgggggat 
      841 ccactagtaa cggccgccag tgtgctggaa ttctgcagat ccaatgccct ggctcacaaa 
      901 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag 
      961 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt 
     1021 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag 
     1081 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg 
     1141 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga 
     1201 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta 
     1261 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta 
     1321 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt 
     1381 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 
     1441 caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc 
     1501 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg 
     1561 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 
     1621 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 
     1681 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 
     1741 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata 
     1801 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 
     1861 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc 
     1921 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 
     1981 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 
     2041 cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 
     2101 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 
     2161 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 
     2221 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 
     2281 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 
     2341 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 
     2401 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 
     2461 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 
     2521 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 
     2581 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 
     2641 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 
     2701 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 
     2761 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 
     2821 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 
     2881 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 
     2941 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 
     3001 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 
     3061 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 
     3121 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 
     3181 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 
     3241 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 
     3301 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 
     3361 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 
     3421 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 
     3481 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 
     3541 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 
     3601 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 
     3661 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 
     3721 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 
     3781 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 
     3841 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga 
     3901 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg 
     3961 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     4021 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     4081 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     4141 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     4201 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     4261 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc 
     4321 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg 
     4381 gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg 
     4441 aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg 
     4501 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt 
     4561 gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc 
     4621 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt 
     4681 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag 
     4741 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg 
     4801 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc 
     4861 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc 
     4921 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg 
     4981 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc 
     5041 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg 
     5101 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct 
     5161 cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg 
     5221 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact 
     5281 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag 
     5341 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg 
     5401 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga 
     5461 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg 
     5521 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa 
     5581 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa 
//
