LOCUS       pCAGGSMtPHGPx           6397 bp    DNA     circular     16-APR-2014
DEFINITION  LMBP 4432.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4432
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 16-APR-2014
COMMENT     VNTDATE|614774635|
COMMENT     VNTNAME|pCAGGSMtPHGPx|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGSMtPHGPx" 0 6397 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "ApaI" "BamHI" "HindIII" "PvuII" "NcoI" "XhoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 615 614 1 6397 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaI" "gggccc" 6 0 3 2310 0 5268 0 5627 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 3 960 0 1635 0 2767 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1498 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuII" "cagctg" 4 0 4 169 0 370 0 2133 0 2267 
COMMENT            0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 669 0 1643 0 2523 0 5139 
COMMENT            0 0 "") #5=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 816 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "polyoma DNA" 21 0 0 1632 2379 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1634..2379") 
COMMENT        #7=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3767 4627 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3767..4627)") 
COMMENT        #8=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT            0 1032 1123 0 (CStringList) (CStringList) 1 1 1 1 "1032..1123") 
COMMENT        #9=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 0 
COMMENT            1124 1480 0 (CStringList) (CStringList) 1 1 1 1 "1124..1480") 
COMMENT        #10=(CFSignal (CObList) "polyoma ori" 33 0 0 2267 2379 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2267..2379") 
COMMENT        #11=(CFSignal (CObList) "SV40 ori" 33 0 0 2530 2605 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2530..2605") 
COMMENT        #12=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2636 2710 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2636..2710") 
COMMENT        #13=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2636 2710 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2636..2710)") 
COMMENT        #14=(CFSignal (CObList) "pMB1 ori" 33 0 1 2825 3226 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2825..3226)") 
COMMENT        #15=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4763 5140 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4763..5140") 
COMMENT        #16=(CFSignal (CObList) "AG promoter" 29 0 0 5146 90 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5146..90") 
COMMENT        #17=(CFSignal (CObList) "intron AG promoter" 15 0 0 5515 42 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5515..42") 
COMMENT        #18=(CFSignal (CObList) "pPHGPx" 4 0 0 205 717 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "205..717") 
COMMENT        #19=(CFSignal (CObList) "5' UTR of hMnSOD" 21 0 0 99 132 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #20=(CFSignal (CObList) "MTS of hMnSOD" 4 0 0 133 204 0 (CStringList) 
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COMMENT        #21=(CFSignal (CObList) "3' UTR of pPHGPx" 21 0 0 718 936 0 
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COMMENT        #23=(CFSignal (CObList) "Last modified: 16-APR-2014" 2 0 0 0 0 0 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 01/11/12 10:43" 1) 
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COMMENT                          "Last Modified: 04/16/14 02:41" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6397 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 15-APR-2014" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT        #355=(CSampFrag "pCAGGSPy" 4590 2767 "BamHI" 960 "BamHI" (LOGPEN 0 0 0) 
COMMENT              2.47776e-316 9.86407e-313) 
COMMENT        #356=(CSampFrag "pCAGGSPy" 1132 1635 "BamHI" 2767 "BamHI" (LOGPEN 0 0 0) 
COMMENT              2.41912e-316 8.27275e-125) 
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COMMENT              2.41912e-316 1.89131e-307))))
FEATURES             Location/Qualifiers
     misc_feature    1632..2379
                     /vntifkey="21"
                     /label=polyoma\DNA
     CDS             complement(3767..4627)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     terminator      1032..1123
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_feature    1124..1480
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     rep_origin      2267..2379
                     /vntifkey="33"
                     /label=polyoma\ori
     rep_origin      2530..2605
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      2636..2710
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(2636..2710)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(2825..3226)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        4763..5140
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     CDS             133..204
                     /vntifkey="4"
                     /label=MTS\of\hMnSOD
     promoter        5146..90
                     /vntifkey="29"
                     /label=AG\promoter
     intron          5515..42
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             205..717
                     /vntifkey="4"
                     /label=pPHGPx
     misc_feature    99..132
                     /vntifkey="21"
                     /label=5'\UTR\of\hMnSOD
     misc_feature    718..936
                     /vntifkey="21"
                     /label=3'\UTR\of\pPHGPx
BASE COUNT     1418 a      1682 c      1743 g      1554 t 
ORIGIN
        1 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg 
       61 gttattgtgc tgtctcatca ttttggcaaa gaattcggcg gcggcatcag cggtagcacc 
      121 agcactagca gcatgttgag ccgggcagtg tgcggcacca gcaggcagct ggctccggtt 
      181 ttggggtatc tgggctccag gcagatgtgc gcgtcccgcg acgactggcg atgtgctcgc 
      241 tccatgcacg aattctcagc caaggacatc gacgggcaca tggtgaacct ggacaagtac 
      301 cggggctacg tgtgcatcgt caccaatgtg gcctctcaat gaggcaagac ggaggtaaac 
      361 tacactcagc tggtcgacct gcacgcccga tatgctgagt gtggtttacg gattctggcc 
      421 ttcccttgca accagtttgg gaggcaggag ccagggagtg atgctgagat caaagaattt 
      481 gctgctggct acaacgtcaa atttgatatg ttcagcaaga tctgtgtgaa tggggacgat 
      541 gcccaccctc tgtggaagtg gatgaaagtc cagcccaagg ggaggggcat gctgggaaat 
      601 gctatcaaat ggaactttac caagttcctc attgataaga acggctgtgt ggtgaagcgg 
      661 tacggtccca tggaagagcc ccaggtcata gagaaggacc tgccgtgcta cctctagctc 
      721 aacaagtgtg tgctgagcct gctgcctgtg ccctcagagc cttccacctg gcacccatga 
      781 cagtctgcct aaaaaccagc cctggtgggg cagactcgag aacctggcgt gcaaccccgc 
      841 cagaggaagg tcccttggcc cagcttgcag ctcgcacccc accccaggct gccttgtggg 
      901 aataaaccat acaaattggc taaaaaaaaa aaaaaaaccc gaattcctgc agcccggggg 
      961 atccactagt aacggccgcc agtgtgctgg aattctgcag atccaatgcc ctggctcaca 
     1021 aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg 
     1081 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt 
     1141 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg 
     1201 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt 
     1261 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata 
     1321 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt 
     1381 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac 
     1441 tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc 
     1501 ttgaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 
     1561 acaaataaag catttttatc actgcattct agttgtggtt tgtccaaact catcaatgta 
     1621 tcttatcatg tctggatccc gccatggtat caacgccata tttctattta cagtagggac 
     1681 ctcttcgttg tgtaggtacc gctgtattcc tagggaaata gtagaggcac cttgaactgt 
     1741 ctgcatcagc catatagccc ccgctgttcg acttacaaac acaggcacag tactgacaaa 
     1801 cccatacacc tcctctgaaa tacccatagt tgctagggct gtctccgaac tcattacacc 
     1861 ctaccaagtg agagctgtaa tttcgcgatc aagggcagcg agggcttctc cagataaaat 
     1921 agcttctgcc gagagtcccg taagggtaga cacttcagct aatccctcga tgaggtctac 
     1981 tagaatagtc agtgcggctc ccattttgaa aattcactta cttgatcagc ttcagaagat 
     2041 gggcgagggc ctccaacaca gtaattttcc tcccgactct taaaatagaa aatgtcaagt 
     2101 cagttaagga ggaagtggac taactgacgc agctggccgt gcgacatcct cttttaatta 
     2161 gttgctaggc aacgccctcc agagggcgtg tggttttgca agaggaagca aaagcctctc 
     2221 cacccaggcc tagaatgttt ccacccaatc attactatga caacagctgt tttttttagt 
     2281 attaagcaga ggccggggac ccctgggccc gcttactctg gagaaaaaga agagaggcat 
     2341 tgtagaggct tccagaggca acttgtcaaa acaggactgc ttctatttct gtcacactgt 
     2401 ctggccctgt cacaaggtcc agcacctcca tacccccttt aataagcagt ttgggaacgg 
     2461 gtgcgggtct tactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     2521 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 
     2581 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt 
     2641 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 
     2701 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 
     2761 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 
     2821 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 
     2881 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag 
     2941 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 
     3001 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 
     3061 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 
     3121 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 
     3181 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 
     3241 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 
     3301 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 
     3361 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 
     3421 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 
     3481 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 
     3541 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 
     3601 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 
     3661 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 
     3721 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 
     3781 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 
     3841 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 
     3901 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 
     3961 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 
     4021 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 
     4081 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 
     4141 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 
     4201 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 
     4261 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 
     4321 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 
     4381 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 
     4441 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 
     4501 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 
     4561 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 
     4621 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 
     4681 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 
     4741 cccgaaaagt gccacctggg tcgacattga ttattgacta gttattaata gtaatcaatt 
     4801 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 
     4861 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 
     4921 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 
     4981 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 
     5041 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 
     5101 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg 
     5161 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt 
     5221 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggccccc cccaggcggg 
     5281 gcggggcggg gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc agccaatcag 
     5341 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa 
     5401 aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc 
     5461 gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc 
     5521 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt 
     5581 tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg 
     5641 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg 
     5701 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg 
     5761 cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac 
     5821 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc 
     5881 gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt 
     5941 gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag 
     6001 gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaaggggcg 
     6061 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt 
     6121 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa 
     6181 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg 
     6241 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc 
     6301 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc 
     6361 ggggttcggc ttctggcgtg tgaccggcgg ctctaga 
//
