ID pEF-BOS standard; circular DNA; ; 5683 BP. DE LMBP 4456. CC Accession number: LMBP 4456 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 29-OCT-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT rep_origin 207..663 FT /vntifkey="33" FT /label=M13\ori FT /note="phage M13 origin" FT terminator complement(918..1484) FT /vntifkey="43" FT /label=3'\UTR\of\hGM-CSF,\incl.\polyA FT /note="3' UTR of the human GM-CSF gene, incl. polyA" FT promoter complement(1948..3091) FT /vntifkey="29" FT /label=hEF1\promoter FT /note="human elongation factor 1alpha promoter" FT intron complement(1957..2895) FT /vntifkey="15" FT /label=intron\hEF1\promoter FT /note="human elongation factor 1alpha intron" FT CDS complement(3132..3201) FT /vntifkey="4" FT /label=part\of\SV40\small\t-antigen FT /note="part of the SV40 small t-antigen" FT promoter complement(3272..3443) FT /vntifkey="29" FT /label=SV40\early\promoter FT /note="SV40 early promoter" FT rep_origin complement(3240..3315) FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT promoter complement(3505..3588) FT /vntifkey="30" FT /label=lac\promoter FT /note="E. coli lac operon promoter" FT rep_origin complement(3680..4082) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(4623..5483) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" SQ Sequence 5683 BP; 1353 A; 1558 C; 1474 G; 1298 t; tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg 240 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 300 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 360 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 420 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 480 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 540 tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa 600 caggattttc gcctgctggg gcaaaccagc gtggaccgaa ttttaacaaa atattaacgt 660 tttgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 720 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 780 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 840 ccagtcacga cgttgtaaaa cgacggccag tgaattcttt tttttttttt tttttttttt 900 tttttttttt tttttttgga ggcaaacact ttattatcct gaagttcagt tacaatgtga 960 cagtggaggg gacactggtg agtggggggt gaagaggtgg agggtgacag tggagcttcc 1020 tttcacacac aggcctgaca tgattcctcc tgtctgctcc ctcccacatc cccagtcccc 1080 atccagcaag gcaaatgtcc aggcacctgc ctggccctaa aaagagtcac agtcctgacc 1140 ctcgtggggg cagggcaggt gtcccgaggc cacccagaaa aacaggagac acgtcggtga 1200 tgttcgggag tcaaaccatg tcccaaaagt cttaagaaga aaaacgagat tccccagcaa 1260 attcgtggga ccccagggcc atgcctccca gctctggcca cctctgcttg tccaggggcc 1320 tcacagccaa ggggatgcag gggccgctgt gcagtcggct gaggccagag tgaggggtgc 1380 aaggacccag atggggaaca ctgctgttta aatattaaac agggatttct tgtctcccac 1440 gtgggagacc tgatactctc aagggtccca ggtggggacc ctcactctag agtcgcggcc 1500 gcgacctgca ggcgcagaac tggtaggtat ggaagatccc tcgagatcca ttgtgctggc 1560 ggtaggcgag cagcgcctgc ctgaagctgc gggattcccg atcagaaatg agcgccagtc 1620 gtcgtcggct ctcggcaccg aagcgtatga ttctccgcca gcatggcttc ggccagtgcg 1680 tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg cacccccaac 1740 cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa cagtccaggg 1800 ccggcacgga tcactgtatt cggctgcaac tttgtcatgc ttgacacttt atcactgata 1860 aacataatat gtccaccaac ttatcagtga taaagaatcc gcgccagcac aatggatctc 1920 gaggtcgagg gatctctaga gaattcctca cgacacctga aatggaagaa aaaaactttg 1980 aaccactgtc tgaggcttga gaatgaacca agatccaaac tcaaaaaggg caaattccaa 2040 ggagaattac atcaagtgcc aagctggcct aacttcagtc tccacccact cagtgtgggg 2100 aaactccatc gcataaaacc cctcccccca acctaaagac gacgtactcc aaaagctcga 2160 gaactaatcg aggtgcctgg acggcgcccg gtactccgtg gagtcacatg aagcgacggc 2220 tgaggacgga aaggcccttt tcctttgtgt gggtgactca cccgcccgct ctcccgagcg 2280 ccgcgtcctc cattttgagc tccctgcagc agggccggga agcggccatc tttccgctca 2340 cgcaactggt gccgaccggg ccagccttgc cgcccagggc ggggcgatac acggcggcgc 2400 gaggccaggc accagagcag gccggccagc ttgagactac ccccgtccga ttctcggtgg 2460 ccgcgctcgc aggccccgcc tcgccgaaca tgtgcgctgg gacgcacggg ccccgtcgcc 2520 gcccgcggcc ccaaaaaccg aaataccagt gtgcagatct tggcccgcat ttacaagact 2580 atcttgccag aaaaaaagcg tcgcagcagg tcatcaaaaa ttttaaatgg ctagagactt 2640 atcgaaagca gcgagacagg cgcgaaggtg ccaccagatt cgcacgcggc ggccccagcg 2700 cccaggccag gcctcaactc aagcacgagg cgaaggggct ccttaagcgc aaggcctcga 2760 actctcccac ccacttccaa cccgaagctc gggatcaaga atcacgtact gcagccaggt 2820 ggaagtaatt caaggcacgc aagggccata acccgtaaag aggccaggcc cgcgggaacc 2880 acacacggca cttacctgtg ttctggcggc aaacccgttg cgaaaaagaa cgttcacggc 2940 gactactgca cttatatacg gttctccccc accctcggga aaaaggcgga gccagtacac 3000 gacatcactt tcccagttta ccccgcgcca ccttctctag gcaccggttc aattgccgac 3060 ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc tctgcccact gacgggcacc 3120 ggagcctcac gaggcactcc tttcaagacc tagaaggtcc attagctgca aagattcctc 3180 tctgtttaaa actttatcca tctttgcaaa gctttttgca aaagcctagg cctccaaaaa 3240 agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa 3300 taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg 3360 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca 3420 tacttctgcc tgctggggag cctaagcttg gcgtaatcat ggtcatagct gtttcctgtg 3480 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 3540 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 3600 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 3660 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 3720 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 3780 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 3840 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 3900 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 3960 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 4020 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 4080 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 4140 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 4200 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 4260 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 4320 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 4380 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 4440 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 4500 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 4560 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 4620 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 4680 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 4740 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 4800 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 4860 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 4920 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 4980 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 5040 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 5100 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 5160 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 5220 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 5280 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 5340 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 5400 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 5460 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 5520 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 5580 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 5640 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc 5683 //