LOCUS       pBlueBachsGCA1His 7116 bp dsDNACircular
DEFINITION  LMBP 4464.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     CDS             3906..4766
                     /label=AMP
     CDS             complement(5702..6814)
                     /label=5'\lacZ\Fragment
     misc_feature    2336..2353
                     /label=6xHIS
     CDS             complement(2640..3518)
                     /label=Recombination\sequence
     rep_origin      5307..5701
                     /label=pMB1\ori
     CDS             191..2260
                     /label=hsGCAlpha1
     promoter        complement(6815..7116)
                     /label=ETL\promoter
     terminator      2378..2452
                     /label=SV40\polyA\early
     promoter        5..88
                     /label=PH\promoter
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt 
       61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca 
      121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat 
      181 tgcccttacc atgttctgca cgaagctcaa ggatctcaag atcacaggag agtgtccttt 
      241 ctccttactg gcaccaggtc aagttcctaa cgagtcttca gaggaggcag caggaagctc 
      301 agagagctgc aaagcaaccg tgcccatctg tcaagacatt cctgagaaga acatacaaga 
      361 aagtcttcct caaagaaaaa ccagtcggag ccgagtctat cttcacactt tggcagagag 
      421 tatttgcaaa ctgattttcc cagagtttga acggctgaat gttgcacttc agagaacatt 
      481 ggcaaagcac aaaataaaag aaagcaggaa atctttggaa agagaagact ttgaaaaaac 
      541 aattgcagag caagcagttg cagcaggagt tccagtggag gttatcaaag aatctcttgg 
      601 tgaagaggtt tttaaaatat gttacgagga agatgaaaac atccttgggg tggttggagg 
      661 cacccttaaa gattttttaa acagcttcag tacccttctg aaacagagca gccattgcca 
      721 agaagcagga aaaaggggca ggcttgagga cgcctccatt ctatgcctgg ataaggagga 
      781 tgattttcta catgtttact acttcttccc taagagaacc acctccctga ttcttcccgg 
      841 catcataaag gcagctgctc acgtattata tgaaacggaa gtggaagtgt cgttaatgcc 
      901 tccctgcttc cataatgatt gcagcgagtt tgtgaatcag ccctacttgt tgtactccgt 
      961 tcacatgaaa agcaccaagc catccctgtc ccccagcaaa ccccagtcct cgctggtgat 
     1021 tcccacatcg ctattctgca agacatttcc attccatttc atgtttgaca aagatatgac 
     1081 aattctgcaa tttggcaatg gcatcagaag gctgatgaac aggagagact ttcaaggaaa 
     1141 gcctaatttt gaagaatact ttgaaattct gactccaaaa atcaaccaga cgtttagcgg 
     1201 gatcatgact atgttgaata tgcagtttgt tgtacgagtg aggagatggg acaactctgt 
     1261 gaagaaatct tcaagggtta tggacctcaa aggccaaatg atctacattg ttgaatccag 
     1321 tgcaatcttg tttttggggt caccctgtgt ggacagatta gaagatttta caggacgagg 
     1381 gctctacctc tcagacatcc caattcacaa tgcactgagg gatgtggtct taatagggga 
     1441 acaagcccga gctcaagatg gcctgaagaa gaggctgggg aagctgaagg ctacccttga 
     1501 gcaagcccac caagccctgg aggaggagaa gaaaaagaca gtagaccttc tgtgctccat 
     1561 atttccctgt gaggttgctc agcagctgtg gcaagggcaa gttgtgcaag ccaagaagtt 
     1621 cagtaatgtc accatgctct tctcagacat cgttgggttc actgccatct gctcccagtg 
     1681 ctcaccgctg caggtcatca ccatgctcaa tgcactgtac actcgcttcg accagcagtg 
     1741 tggagagctg gatgtctaca aggtggagac cattggcgat gcctattgtg tagctggggg 
     1801 attacacaaa gagagtgata ctcatgctgt tcagatagcg ctgatggccc tgaagatgat 
     1861 ggagctctct gatgaagtta tgtctcccca tggagaacct atcaagatgc gaattggact 
     1921 gcactctgga tcagtttttg ctggcgtcgt tggagttaaa atgccccgtt actgtctttt 
     1981 tggaaacaat gtcactctgg ctaacaaatt tgagtcctgc agtgtaccac gaaaaatcaa 
     2041 tgtcagccca acaacttaca gattactcaa agactgtcct ggtttcgtgt ttacccctcg 
     2101 atcaagggag gaacttccac caaacttccc tagtgaaatc cccggaatct gccattttct 
     2161 ggatgcttac caacaaggaa caaactcaaa accatgcttc caaaagaaag atgtggaaga 
     2221 tggcaatgcc aattttttag gcaaagcatc aggaatagat aagggcaatt cgaagcttag 
     2281 gcctggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca 
     2341 tcaccatcac cattgacgtc tctagcttgg agtcgacaac ttgtttattg cagcttataa 
     2401 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 
     2461 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catctctcga ctctgctgaa 
     2521 gaggaggaaa ttctccttga agtttccctg gtgttcaaag taaaggagtt tgcaccagac 
     2581 gcacctctgt tcactggtcc ggcgtattaa aacacgatac attgttatta gtacatttat 
     2641 taagcgctag attctgtgcg ttgttgattt acagacaatt gttgtacgta ttttaataat 
     2701 tcattaaatt tataatcttt agggtggtat gttagagcga aaatcaaatg attttcagcg 
     2761 tctttatatc tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag gtttcgatta 
     2821 gtttcaaaca agggttgttt ttccgaaccg atggctggac tatctaatgg attttcgctc 
     2881 aacgccacaa aacttgccaa atcttgtagc agcaatctag ctttgtcgat attcgtttgt 
     2941 gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt 
     3001 tcatcactgt cgttagtgta caattgactc gacgtaaaca cgttaaataa agcctggaca 
     3061 tatttaacat cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc ccaaccctcg 
     3121 tcgttagaag ttgcttccga agacgatttt gccatagcca cacgacgcct attaattgtg 
     3181 tcggctaaca cgtccgcgat caaatttgta gttgagcttt ttggaattat ttctgattgc 
     3241 gggcgttttt gggcgggttt caatctaact gtgcccgatt ttaattcaga caacacgtta 
     3301 gaaagcgatg gtgcaggcgg tggtaacatt tcagacggca aatctactaa tggcggcggt 
     3361 ggtggagctg atgataaatc taccatcggt ggaggcgcag gcggggctgg cggcggaggc 
     3421 ggaggcggag gtggtggcgg tgatgcagac ggcggtttag gctcaaatgt ctctttaggc 
     3481 aacacagtcg gcacctcaac tattgtactg gtttcgggcg atggtgcact ctcagtacaa 
     3541 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 
     3601 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 
     3661 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 
     3721 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 
     3781 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 
     3841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 
     3901 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 
     3961 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 
     4021 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 
     4081 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 
     4141 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 
     4201 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 
     4261 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 
     4321 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 
     4381 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 
     4441 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 
     4501 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 
     4561 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 
     4621 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 
     4681 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 
     4741 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 
     4801 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 
     4861 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 
     4921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 
     4981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 
     5041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 
     5101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 
     5161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 
     5221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 
     5281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 
     5341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 
     5401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 
     5461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 
     5521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 
     5581 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 
     5641 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 
     5701 catcctgcac catcgtctgc tcatccatga cctgaccatg cagaggatga tgctcgtgac 
     5761 ggttaacgcc tcgaatcagc aacggcttgc cgttcagcag cagcagacca ttttcaatcc 
     5821 gcacctcgcg gaaaccgaca tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt 
     5881 gcagttcaac caccgcacga tagagattcg ggatttcggc gctccacagt ttcgggtttt 
     5941 cgacgttcag acgtagtgtg acgcgatcgg cataaccacc acgctcatcg ataatttcac 
     6001 cgccgaaagg cgcggtgccg ctggcgacct gcgtttcacc ctgccataaa gaaactgtta 
     6061 cccgtaggta gtcacgcaac tcgccgcaca tctgaacttc agcctccagt acagcgcggc 
     6121 tgaaatcatc attaaagcga gtggcaacat ggaaatcgct gatttgtgta gtcggtttat 
     6181 gcagcaacga gacgtcacgg aaaatgccgc tcatccgcca catatcctga tcttccagat 
     6241 aactgccgtc actccagcgc agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa 
     6301 atgcgctcag gtcaaattca gacggcaaac gactgtcctg gccgtaaccg acccagcgcc 
     6361 cgttgcacca cagatgaaac gccgagttaa cgccatcaaa aataattcgc gtctggcctt 
     6421 cctgtagcca gctttcatca acattaaatg tgagcgagta acaacccgtc ggattctccg 
     6481 tgggaacaaa cggcggattg accgtaatgg gataggtcac gttggtgtag atgggcgcat 
     6541 cgtaaccgtg catctgccag tttgagggga cgacgacagt atcggcctca ggaagatcgc 
     6601 actccagcca gctttccggc accgcttctg gtgccggaaa ccaggcaaag cgccattcgc 
     6661 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc 
     6721 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc 
     6781 agtcacgacg ttgtaaaacg acgggatcta tcatttttag cagtgattct aattgcagct 
     6841 gctctttgat acaactaatt ttacgacgac gatgcgagct tttattcaac cgagcgtgca 
     6901 tgtttgcaat cgtgcaagcg ttatcaattt ttcattatcg tattgttgca catcaacagg 
     6961 ctggacacca cgttgaactc gccgcagttt tgcggcaagt tggacccgcc gcgcatccaa 
     7021 tgcaaacttt ccgacattct gttgcctacg aacgattgat tctttgtcca ttgatcgaag 
     7081 cgagtgcctt cgactttttc gtgtccagtg tggctt 
//
