ID pBlueBacmsGCB1 standard; circular DNA; ; 6926 BP. DE LMBP 4466. CC Accession number: LMBP 4466 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 11-DEC-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 7..95 FT /vntifkey="29" FT /label=AcNPV-PH\promoter FT /note="AcNPV polyhedrin promoter" FT CDS 194..2056 FT /vntifkey="4" FT /label=msGCB1 FT /note="mouse soluble guanylate cyclase ß1 coding sequence" FT terminator 2188..2262 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(2188..2262) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT CDS complement(2450..3328) FT /vntifkey="4" FT /label=3'\AcNPV\ORF\1629 FT /note="3' fragment of the AcNPV open reading frame 1629" FT CDS 3716..4576 FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT rep_origin 5117..5511 FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(5512..6624) FT /vntifkey="4" FT /label=5'\lacZ\fragment FT /note="5' fragment of the E. coli lacZ coding sequence" FT promoter complement(6625..6899) FT /vntifkey="29" FT /label=AcNPV-ETL\promoter FT /note="AcNPV EcoRI-T large promoter" FT misc_marker 2146..2163 FT /vntifkey="22" FT /label=His-tag FT misc_marker 2095..2136 FT /vntifkey="22" FT /label=V5\epitope SQ Sequence 6926 BP; 1787 A; 1655 C; 1695 G; 1789 t; gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt 60 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca 120 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat 180 tgcccttgac accatgtacg gtttcgtgaa ccatgccctg gagctgctgg tgatccgcaa 240 ttatggtccc gaggtgtggg aagacatcaa aaaagaggca cagctggatg aagaaggcca 300 gtttcttgtc agaataatat acgatgattc caaaacatat gacctggtgg ctgctgcaag 360 caaagtcctc aacctcaatg ctggcgaaat cctgcagatg tttgggaaga tgtttttcgt 420 cttctgccag gagtctggct atgataccat cttgcgtgtc ctgggatcta atgtcagaga 480 gtttttgcag aacctcgatg ccctgcatga ccacctcgcc accatttacc cagggatgcg 540 cgcgccttcc ttcaggtgca ccgatgcgga gaaaggcaaa gggctcatcc tgcactacta 600 ctcggaaaga gaggggcttc aggacatcgt gatcgggatt atcaagactg ttgctcaaca 660 gatacacggc actgagatag acatgaaggt gattcagcaa agaaatgaag aatgtgatca 720 cacccagttt ttaattgaag aaaaggaatc aaaagaagag gatttttatg aagatctgga 780 taggtttgaa gagaatggta cccaggaatc acgtatcagc ccttacacct tctgcaaagc 840 atttcctttt cacatcatat ttgaccggaa cctagtggtc actcagtgtg gcaatgccat 900 ctacagagtg ctcccccagc tccagcctgg gaactgcagc cttctgtctg tcttctctct 960 ggtccgccct cacatcgaca tcagtttcca tgggattctt tcacacatca atacagtctt 1020 tgtactgaga agcaaggaag ggttgctgga tgtggagaaa cttgagtgtg aggatgaact 1080 gactggagca gagattagct gcttacgtct caaaggccaa atgatctact taccagaagc 1140 agatagcatc ctcttcctct gttcaccaag tgtgatgaac ctggacgacc taacaagaag 1200 aggcctgtat ctgagtgaca tccctctcca cgatgctacc cgagacctgg ttcttttggg 1260 agaacagttc cgggaggagt acaaactgac acaagagctg gaaatcctca ccgacaggct 1320 gcagctcaca ctgagagcct tggaggatga gaagaaaaag acagacacat tgctgtattc 1380 tgtccttcct ccatctgttg ccaatgagct gagacacaag cgcccagtgc ctgccaaaag 1440 atacgacaat gtgaccatcc tcttcagcgg cattgtgggc ttcaatgctt tctgtagcaa 1500 gcatgcatct ggagaagggg ccatgaagat tgtcaatctc ctcaacgatc tctacacccg 1560 atttgacaca ctgactgatt cacgaaaaaa cccatttgtt tacaaggtgg aaacagttgg 1620 tgacaagtat atgacagtga gtggcttgcc agaaccttgt atccaccatg cacggtccat 1680 ttgccacctg gctttagaca tgatggaaat tgctggtcaa gttcaagtag atggtgaatc 1740 tgttcagata acaatcggga tccataccgg ggaggtggtg acaggtgtga ttggacagcg 1800 gatgcctcgg tattgtctct ttgggaatac cgtcaacctc acaagcagga cagaaaccac 1860 aggagaaaag ggaaagataa atgtttccga atatacatac aggtgtctca tgtctccaga 1920 aaactcggat ccactgttcc atttggagca cagaggccca gtgtctatga agggcaagaa 1980 ggaaccaatg caagtctggt tcctatccag gaaaaataca ggcacggagg aaacaaatga 2040 ggaggatgaa aactgagtgt caggttgtgg aagggcaatt cgaagcttag gcctggtaag 2100 cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac 2160 cattgacgtc tctagcttgg agtcgacaac ttgtttattg cagcttataa tggttacaaa 2220 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 2280 ggtttgtcca aactcatcaa tgtatcttat catctctcga ctctgctgaa gaggaggaaa 2340 ttctccttga agtttccctg gtgttcaaag taaaggagtt tgcaccagac gcacctctgt 2400 tcactggtcc ggcgtattaa aacacgatac attgttatta gtacatttat taagcgctag 2460 attctgtgcg ttgttgattt acagacaatt gttgtacgta ttttaataat tcattaaatt 2520 tataatcttt agggtggtat gttagagcga aaatcaaatg attttcagcg tctttatatc 2580 tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag gtttcgatta gtttcaaaca 2640 agggttgttt ttccgaaccg atggctggac tatctaatgg attttcgctc aacgccacaa 2700 aacttgccaa atcttgtagc agcaatctag ctttgtcgat attcgtttgt gttttgtttt 2760 gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt tcatcactgt 2820 cgttagtgta caattgactc gacgtaaaca cgttaaataa agcctggaca tatttaacat 2880 cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc ccaaccctcg tcgttagaag 2940 ttgcttccga agacgatttt gccatagcca cacgacgcct attaattgtg tcggctaaca 3000 cgtccgcgat caaatttgta gttgagcttt ttggaattat ttctgattgc gggcgttttt 3060 gggcgggttt caatctaact gtgcccgatt ttaattcaga caacacgtta gaaagcgatg 3120 gtgcaggcgg tggtaacatt tcagacggca aatctactaa tggcggcggt ggtggagctg 3180 atgataaatc taccatcggt ggaggcgcag gcggggctgg cggcggaggc ggaggcggag 3240 gtggtggcgg tgatgcagac ggcggtttag gctcaaatgt ctctttaggc aacacagtcg 3300 gcacctcaac tattgtactg gtttcgggcg atggtgcact ctcagtacaa tctgctctga 3360 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc 3420 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg 3480 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct 3540 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg 3600 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 3660 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag 3720 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 3780 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt 3840 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga 3900 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat 3960 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga 4020 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag 4080 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg 4140 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg 4200 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt 4260 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg 4320 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 4380 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg 4440 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac 4500 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact 4560 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa 4620 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 4680 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 4740 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 4800 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 4860 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca 4920 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 4980 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 5040 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 5100 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 5160 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 5220 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 5280 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 5340 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt 5400 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac 5460 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag catcctgcac 5520 catcgtctgc tcatccatga cctgaccatg cagaggatga tgctcgtgac ggttaacgcc 5580 tcgaatcagc aacggcttgc cgttcagcag cagcagacca ttttcaatcc gcacctcgcg 5640 gaaaccgaca tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt gcagttcaac 5700 caccgcacga tagagattcg ggatttcggc gctccacagt ttcgggtttt cgacgttcag 5760 acgtagtgtg acgcgatcgg cataaccacc acgctcatcg ataatttcac cgccgaaagg 5820 cgcggtgccg ctggcgacct gcgtttcacc ctgccataaa gaaactgtta cccgtaggta 5880 gtcacgcaac tcgccgcaca tctgaacttc agcctccagt acagcgcggc tgaaatcatc 5940 attaaagcga gtggcaacat ggaaatcgct gatttgtgta gtcggtttat gcagcaacga 6000 gacgtcacgg aaaatgccgc tcatccgcca catatcctga tcttccagat aactgccgtc 6060 actccagcgc agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa atgcgctcag 6120 gtcaaattca gacggcaaac gactgtcctg gccgtaaccg acccagcgcc cgttgcacca 6180 cagatgaaac gccgagttaa cgccatcaaa aataattcgc gtctggcctt cctgtagcca 6240 gctttcatca acattaaatg tgagcgagta acaacccgtc ggattctccg tgggaacaaa 6300 cggcggattg accgtaatgg gataggtcac gttggtgtag atgggcgcat cgtaaccgtg 6360 catctgccag tttgagggga cgacgacagt atcggcctca ggaagatcgc actccagcca 6420 gctttccggc accgcttctg gtgccggaaa ccaggcaaag cgccattcgc cattcaggct 6480 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 6540 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 6600 ttgtaaaacg acgggatcta tcatttttag cagtgattct aattgcagct gctctttgat 6660 acaactaatt ttacgacgac gatgcgagct tttattcaac cgagcgtgca tgtttgcaat 6720 cgtgcaagcg ttatcaattt ttcattatcg tattgttgca catcaacagg ctggacacca 6780 cgttgaactc gccgcagttt tgcggcaagt tggacccgcc gcgcatccaa tgcaaacttt 6840 ccgacattct gttgcctacg aacgattgat tctttgtcca ttgatcgaag cgagtgcctt 6900 cgactttttc gtgtccagtg tggctt 6926 //