LOCUS       pBlueBacmsGCB1His        6910 bp    DNA     circular     11-DEC-2007
DEFINITION  LMBP 4467.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4467
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 11-DEC-2007
            
COMMENT     VNTNAME|pBlueBacmsGCB1His|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBlueBacmsGCB1His" 0 6910 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BglII" "HindIII" "XmnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 2434 3312 1 6910 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #3=(CRSite (CStringList) "BglII" "agatct" 2 0 2 152 0 774 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2068 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 3 1742 0 3023 0 3888 0 
COMMENT            0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "AcNPV-PH promoter" 29 0 0 7 95 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "7..95") 
COMMENT        #7=(CFSignal (CObList) "V5 epitope" 22 0 0 2079 2120 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2079..2120") 
COMMENT        #8=(CFSignal (CObList) "His-tag" 22 0 0 2130 2147 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2130..2147") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2172 2246 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2170..2246") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2172 2246 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2170..2246)") 
COMMENT        #11=(CFSignal (CObList) "3' AcNPV ORF 1629" 4 0 1 2434 3312 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2434..3313)") 
COMMENT        #12=(CFSignal (CObList) "ampicillin" 4 0 0 3700 4560 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3700..4560") 
COMMENT        #13=(CFSignal (CObList) "pMB1 ori" 33 0 0 5101 5495 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5101..5495") 
COMMENT        #14=(CFSignal (CObList) "5' lacZ fragment" 4 0 1 5496 6608 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(5496..6608)") 
COMMENT        #15=(CFSignal (CObList) "AcNPV-ETL promoter" 29 0 1 6609 6883 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(6609..6910)") 
COMMENT        #16=(CFSignal (CObList) "msGCB1" 4 0 0 195 2054 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "195..2054")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #17=(CFSignal (CObList) "LMBP 4467" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #18=(CFSignal (CObList) "Last modified: 11-DEC-2007" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT              #20=(CRefLinePar 
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COMMENT                    "pBlueBacmsGCB1His" 2) 5 "" 0 4) 
COMMENT              #21=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pBlueBacmsGCB1His'" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4467" 
COMMENT                          1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 12/11/07 02:22" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6910 bp" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #30=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #32=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
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COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 1 0 0 178) 
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COMMENT                           (CObjectList 
COMMENT                            #35=(CLinePar (CParagraph 0 (15 #17# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 4467") 
COMMENT                     #36=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "Last modified: 11-DEC-2007"))) 
COMMENT                   "Annotations") 
COMMENT              #38=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #39=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #40=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #41=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 195   End: 2054" 1) 
COMMENT                                   #42=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "mouse soluble guanylate cyclase \3371 coding sequence" 
COMMENT                                        1) 
COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   195..2054" 1))) "msGCB1") 
COMMENT                            #45=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2434 (3 #11# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2434  End: 3312 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "3' fragment of the AcNPV open reading frame 1629" 
COMMENT                                        1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2434..3313)" 1))) 
COMMENT                                 "3' AcNPV ORF 1629") 
COMMENT                            #50=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3700 (3 #12# 0) 1 2 2 0 327) 
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COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3700  End: 4560" 1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3700..4560" 1))) "ampicillin") 
COMMENT                            #55=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5496 (3 #14# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #56=(CLinePar 
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COMMENT                                        "Start: 5496  End: 6608 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "5' fragment of the E. coli lacZ coding sequence" 
COMMENT                                        1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(5496..6608)" 1))) 
COMMENT                                 "5' lacZ fragment"))) "CDS (4 total)") 
COMMENT                     #60=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #61=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2079  End: 2120" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "V5 epitope" 1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2079..2120" 1))) "V5 epitope") 
COMMENT                            #66=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2130  End: 2147" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "polyhistidine (6 x His) tag" 1) 
COMMENT                                   #69=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2130..2147" 1))) "His-tag"))) 
COMMENT                          "Misc. Marker (2 total)") 
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COMMENT                           (CObjectList 
COMMENT                            #72=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 7 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7     End: 95  " 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "AcNPV polyhedrin promoter" 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   7..95" 1))) "AcNPV-PH promoter") 
COMMENT                            #77=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #78=(CLinePar 
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COMMENT                                        "Start: 6609  End: 6883 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "AcNPV EcoRI-T large promoter" 1) 
COMMENT                                   #80=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(6609..6910)" 1))) 
COMMENT                                 "AcNPV-ETL promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #82=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                  (CObjectList 
COMMENT                                   #84=(CLinePar 
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COMMENT                                        "Start: 5101  End: 5495" 1) 
COMMENT                                   #85=(CLinePar 
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COMMENT                                        "origin plasmid" 1) 
COMMENT                                   #86=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5101..5495" 1))) "pMB1 ori"))) 
COMMENT                          "Replication Origin (1 total)") 
COMMENT                     #88=(CFolderPar 
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COMMENT                                        "Start: 2172  End: 2246" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "polyA signal of the SV40 early region" 
COMMENT                                        1) 
COMMENT                                   #92=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2170..2246" 1))) "SV40 polyA early") 
COMMENT                            #94=(CFolderPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        "Start: 2172  End: 2246 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "polyA signal of the SV40 late region" 1) 
COMMENT                                   #97=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2170..2246)" 1))) 
COMMENT                                 "SV40 polyA late"))) "Terminator (2 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                                           " N1: 2068 " 1))))) "HindIII: 1 site") 
COMMENT                           1 5 "AAGCTT" "TTCGAA") 
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COMMENT                                           " N1: 152 " 1) 
COMMENT                                     #106=(CLinePar 
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COMMENT                                           " N2: 774 " 1))))) "BglII: 2 sites") 
COMMENT                           1 5 "AGATCT" "TCTAGA") 
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COMMENT                                     #110=(CLinePar 
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COMMENT                                     #111=(CLinePar 
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COMMENT                                           " N3: 3888 " 1))))) "XmnI: 3 sites") 
COMMENT                           5 5 "GAANNNNTTC" "CTTNNNNAAG"))) 
COMMENT                   "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     promoter        7..95
                     /vntifkey="29"
                     /label=AcNPV-PH\promoter
                     /note="AcNPV polyhedrin promoter"
     CDS             195..2054
                     /vntifkey="4"
                     /label=msGCB1
                     /note="mouse soluble guanylate cyclase ß1 coding sequence"
     misc_marker     2079..2120
                     /vntifkey="22"
                     /label=V5\epitope
                     /note="V5 epitope"
     misc_marker     2130..2147
                     /vntifkey="22"
                     /label=His-tag
                     /note="polyhistidine (6 x His) tag"
     terminator      2172..2246
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(2172..2246)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     CDS             complement(2434..3312)
                     /vntifkey="4"
                     /label=3'\AcNPV\ORF\1629
                     /note="3' fragment of the AcNPV open reading frame 1629"
     CDS             3700..4560
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      5101..5495
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(5496..6608)
                     /vntifkey="4"
                     /label=5'\lacZ\fragment
                     /note="5' fragment of the E. coli lacZ coding sequence"
     promoter        complement(6609..6883)
                     /vntifkey="29"
                     /label=AcNPV-ETL\promoter
                     /note="AcNPV EcoRI-T large promoter"
BASE COUNT     1785 a      1654 c      1688 g      1783 t 
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt 
       61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca 
      121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat 
      181 tgcccttgga caccatgtac ggtttcgtga accatgccct ggagctgctg gtgatccgca 
      241 attatggtcc cgaggtgtgg gaagacatca aaaaagaggc acagctggat gaagaaggcc 
      301 agtttcttgt cagaataata tacgatgatt ccaaaacata tgacctggtg gctgctgcaa 
      361 gcaaagtcct caacctcaat gctggcgaaa tcctgcagat gtttgggaag atgtttttcg 
      421 tcttctgcca ggagtctggc tatgatacca tcttgcgtgt cctgggatct aatgtcagag 
      481 agtttttgca gaacctcgat gccctgcatg accacctcgc caccatttac ccagggatgc 
      541 gcgcgccttc cttcaggtgc accgatgcgg agaaaggcaa agggctcatc ctgcactact 
      601 actcggaaag agaggggctt caggacatcg tgatcgggat tatcaagact gttgctcaac 
      661 agatacacgg cactgagata gacatgaagg tgattcagca aagaaatgaa gaatgtgatc 
      721 acacccagtt tttaattgaa gaaaaggaat caaaagaaga ggatttttat gaagatctgg 
      781 ataggtttga agagaatggt acccaggaat cacgtatcag cccttacacc ttctgcaaag 
      841 catttccttt tcacatcata tttgaccgga acctagtggt cactcagtgt ggcaatgcca 
      901 tctacagagt gctcccccag ctccagcctg ggaactgcag ccttctgtct gtcttctctc 
      961 tggtccgccc tcacatcgac atcagtttcc atgggattct ttcacacatc aatacagtct 
     1021 ttgtactgag aagcaaggaa gggttgctgg atgtggagaa acttgagtgt gaggatgaac 
     1081 tgactggagc agagattagc tgcttacgtc tcaaaggcca aatgatctac ttaccagaag 
     1141 cagatagcat cctcttcctc tgttcaccaa gtgtgatgaa cctggacgac ctaacaagaa 
     1201 gaggcctgta tctgagtgac atccctctcc acgatgctac ccgagacctg gttcttttgg 
     1261 gagaacagtt ccgggaggag tacaaactga cacaagagct ggaaatcctc accgacaggc 
     1321 tgcagctcac actgagagcc ttggaggatg agaagaaaaa gacagacaca ttgctgtatt 
     1381 ctgtccttcc tccatctgtt gccaatgagc tgagacacaa gcgcccagtg cctgccaaaa 
     1441 gatacgacaa tgtgaccatc ctcttcagcg gcattgtggg cttcaatgct ttctgtagca 
     1501 agcatgcatc tggagaaggg gccatgaaga ttgtcaatct cctcaacgat ctctacaccc 
     1561 gatttgacac actgactgat tcacgaaaaa acccatttgt ttacaaggtg gaaacagttg 
     1621 gtgacaagta tatgacagtg agtggcttgc cagaaccttg tatccaccat gcacggtcca 
     1681 tttgccacct ggctttagac atgatggaaa ttgctggtca agttcaagta gatggtgaat 
     1741 ctgttcagat aacaatcggg atccataccg gggaggtggt gacaggtgtg attggacagc 
     1801 ggatgcctcg gtattgtctc tttgggaata ccgtcaacct cacaagcagg acagaaacca 
     1861 caggagaaaa gggaaagata aatgtttccg aatatacata caggtgtctc atgtctccag 
     1921 aaaactcgga tccactgttc catttggagc acagaggccc agtgtctatg aagggcaaga 
     1981 aggaaccaat gcaagtctgg ttcctatcca ggaaaaatac aggcacggag gaaacaaatg 
     2041 aggaggatga aaacaagggc aattcgaagc ttaggcctgg taagcctatc cctaaccctc 
     2101 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga cgtctctagc 
     2161 ttggagtcga caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 
     2221 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 
     2281 tcaatgtatc ttatcatctc tcgactctgc tgaagaggag gaaattctcc ttgaagtttc 
     2341 cctggtgttc aaagtaaagg agtttgcacc agacgcacct ctgttcactg gtccggcgta 
     2401 ttaaaacacg atacattgtt attagtacat ttattaagcg ctagattctg tgcgttgttg 
     2461 atttacagac aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg 
     2521 gtatgttaga gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa 
     2581 atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga 
     2641 accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg 
     2701 tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac 
     2761 gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg 
     2821 actcgacgta aacacgttaa ataaagcctg gacatattta acatcgggcg tgttagcttt 
     2881 attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga 
     2941 ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt 
     3001 tgtagttgag ctttttggaa ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct 
     3061 aactgtgccc gattttaatt cagacaacac gttagaaagc gatggtgcag gcggtggtaa 
     3121 catttcagac ggcaaatcta ctaatggcgg cggtggtgga gctgatgata aatctaccat 
     3181 cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc 
     3241 agacggcggt ttaggctcaa atgtctcttt aggcaacaca gtcggcacct caactattgt 
     3301 actggtttcg ggcgatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc 
     3361 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca 
     3421 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg 
     3481 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat 
     3541 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga 
     3601 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 
     3661 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 
     3721 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 
     3781 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 
     3841 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 
     3901 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 
     3961 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 
     4021 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 
     4081 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 
     4141 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 
     4201 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 
     4261 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 
     4321 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 
     4381 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 
     4441 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 
     4501 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 
     4561 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 
     4621 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 
     4681 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 
     4741 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 
     4801 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 
     4861 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 
     4921 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 
     4981 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 
     5041 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 
     5101 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 
     5161 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 
     5221 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 
     5281 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 
     5341 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 
     5401 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 
     5461 acgaccgagc gcagcgagtc agtgagcgag gaagcatcct gcaccatcgt ctgctcatcc 
     5521 atgacctgac catgcagagg atgatgctcg tgacggttaa cgcctcgaat cagcaacggc 
     5581 ttgccgttca gcagcagcag accattttca atccgcacct cgcggaaacc gacatcgcag 
     5641 gcttctgctt caatcagcgt gccgtcggcg gtgtgcagtt caaccaccgc acgatagaga 
     5701 ttcgggattt cggcgctcca cagtttcggg ttttcgacgt tcagacgtag tgtgacgcga 
     5761 tcggcataac caccacgctc atcgataatt tcaccgccga aaggcgcggt gccgctggcg 
     5821 acctgcgttt caccctgcca taaagaaact gttacccgta ggtagtcacg caactcgccg 
     5881 cacatctgaa cttcagcctc cagtacagcg cggctgaaat catcattaaa gcgagtggca 
     5941 acatggaaat cgctgatttg tgtagtcggt ttatgcagca acgagacgtc acggaaaatg 
     6001 ccgctcatcc gccacatatc ctgatcttcc agataactgc cgtcactcca gcgcagcacc 
     6061 atcaccgcga ggcggttttc tccggcgcgt aaaaatgcgc tcaggtcaaa ttcagacggc 
     6121 aaacgactgt cctggccgta accgacccag cgcccgttgc accacagatg aaacgccgag 
     6181 ttaacgccat caaaaataat tcgcgtctgg ccttcctgta gccagctttc atcaacatta 
     6241 aatgtgagcg agtaacaacc cgtcggattc tccgtgggaa caaacggcgg attgaccgta 
     6301 atgggatagg tcacgttggt gtagatgggc gcatcgtaac cgtgcatctg ccagtttgag 
     6361 gggacgacga cagtatcggc ctcaggaaga tcgcactcca gccagctttc cggcaccgct 
     6421 tctggtgccg gaaaccaggc aaagcgccat tcgccattca ggctgcgcaa ctgttgggaa 
     6481 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca 
     6541 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggga 
     6601 tctatcattt ttagcagtga ttctaattgc agctgctctt tgatacaact aattttacga 
     6661 cgacgatgcg agcttttatt caaccgagcg tgcatgtttg caatcgtgca agcgttatca 
     6721 atttttcatt atcgtattgt tgcacatcaa caggctggac accacgttga actcgccgca 
     6781 gttttgcggc aagttggacc cgccgcgcat ccaatgcaaa ctttccgaca ttctgttgcc 
     6841 tacgaacgat tgattctttg tccattgatc gaagcgagtg ccttcgactt tttcgtgtcc 
     6901 agtgtggctt 
//
