ID pBlueBacmsGCB1His standard; circular DNA; ; 6910 BP. DE LMBP 4467. CC Accession number: LMBP 4467 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 11-DEC-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 7..95 FT /vntifkey="29" FT /label=AcNPV-PH\promoter FT /note="AcNPV polyhedrin promoter" FT CDS 195..2054 FT /vntifkey="4" FT /label=msGCB1 FT /note="mouse soluble guanylate cyclase ß1 coding sequence" FT misc_marker 2079..2120 FT /vntifkey="22" FT /label=V5\epitope FT /note="V5 epitope" FT misc_marker 2130..2147 FT /vntifkey="22" FT /label=His-tag FT /note="polyhistidine (6 x His) tag" FT terminator 2172..2246 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(2172..2246) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT CDS complement(2434..3312) FT /vntifkey="4" FT /label=3'\AcNPV\ORF\1629 FT /note="3' fragment of the AcNPV open reading frame 1629" FT CDS 3700..4560 FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT rep_origin 5101..5495 FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(5496..6608) FT /vntifkey="4" FT /label=5'\lacZ\fragment FT /note="5' fragment of the E. coli lacZ coding sequence" FT promoter complement(6609..6883) FT /vntifkey="29" FT /label=AcNPV-ETL\promoter FT /note="AcNPV EcoRI-T large promoter" SQ Sequence 6910 BP; 1785 A; 1654 C; 1688 G; 1783 t; gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt 60 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca 120 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat 180 tgcccttgga caccatgtac ggtttcgtga accatgccct ggagctgctg gtgatccgca 240 attatggtcc cgaggtgtgg gaagacatca aaaaagaggc acagctggat gaagaaggcc 300 agtttcttgt cagaataata tacgatgatt ccaaaacata tgacctggtg gctgctgcaa 360 gcaaagtcct caacctcaat gctggcgaaa tcctgcagat gtttgggaag atgtttttcg 420 tcttctgcca ggagtctggc tatgatacca tcttgcgtgt cctgggatct aatgtcagag 480 agtttttgca gaacctcgat gccctgcatg accacctcgc caccatttac ccagggatgc 540 gcgcgccttc cttcaggtgc accgatgcgg agaaaggcaa agggctcatc ctgcactact 600 actcggaaag agaggggctt caggacatcg tgatcgggat tatcaagact gttgctcaac 660 agatacacgg cactgagata gacatgaagg tgattcagca aagaaatgaa gaatgtgatc 720 acacccagtt tttaattgaa gaaaaggaat caaaagaaga ggatttttat gaagatctgg 780 ataggtttga agagaatggt acccaggaat cacgtatcag cccttacacc ttctgcaaag 840 catttccttt tcacatcata tttgaccgga acctagtggt cactcagtgt ggcaatgcca 900 tctacagagt gctcccccag ctccagcctg ggaactgcag ccttctgtct gtcttctctc 960 tggtccgccc tcacatcgac atcagtttcc atgggattct ttcacacatc aatacagtct 1020 ttgtactgag aagcaaggaa gggttgctgg atgtggagaa acttgagtgt gaggatgaac 1080 tgactggagc agagattagc tgcttacgtc tcaaaggcca aatgatctac ttaccagaag 1140 cagatagcat cctcttcctc tgttcaccaa gtgtgatgaa cctggacgac ctaacaagaa 1200 gaggcctgta tctgagtgac atccctctcc acgatgctac ccgagacctg gttcttttgg 1260 gagaacagtt ccgggaggag tacaaactga cacaagagct ggaaatcctc accgacaggc 1320 tgcagctcac actgagagcc ttggaggatg agaagaaaaa gacagacaca ttgctgtatt 1380 ctgtccttcc tccatctgtt gccaatgagc tgagacacaa gcgcccagtg cctgccaaaa 1440 gatacgacaa tgtgaccatc ctcttcagcg gcattgtggg cttcaatgct ttctgtagca 1500 agcatgcatc tggagaaggg gccatgaaga ttgtcaatct cctcaacgat ctctacaccc 1560 gatttgacac actgactgat tcacgaaaaa acccatttgt ttacaaggtg gaaacagttg 1620 gtgacaagta tatgacagtg agtggcttgc cagaaccttg tatccaccat gcacggtcca 1680 tttgccacct ggctttagac atgatggaaa ttgctggtca agttcaagta gatggtgaat 1740 ctgttcagat aacaatcggg atccataccg gggaggtggt gacaggtgtg attggacagc 1800 ggatgcctcg gtattgtctc tttgggaata ccgtcaacct cacaagcagg acagaaacca 1860 caggagaaaa gggaaagata aatgtttccg aatatacata caggtgtctc atgtctccag 1920 aaaactcgga tccactgttc catttggagc acagaggccc agtgtctatg aagggcaaga 1980 aggaaccaat gcaagtctgg ttcctatcca ggaaaaatac aggcacggag gaaacaaatg 2040 aggaggatga aaacaagggc aattcgaagc ttaggcctgg taagcctatc cctaaccctc 2100 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga cgtctctagc 2160 ttggagtcga caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 2220 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 2280 tcaatgtatc ttatcatctc tcgactctgc tgaagaggag gaaattctcc ttgaagtttc 2340 cctggtgttc aaagtaaagg agtttgcacc agacgcacct ctgttcactg gtccggcgta 2400 ttaaaacacg atacattgtt attagtacat ttattaagcg ctagattctg tgcgttgttg 2460 atttacagac aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg 2520 gtatgttaga gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa 2580 atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga 2640 accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg 2700 tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac 2760 gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg 2820 actcgacgta aacacgttaa ataaagcctg gacatattta acatcgggcg tgttagcttt 2880 attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga 2940 ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt 3000 tgtagttgag ctttttggaa ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct 3060 aactgtgccc gattttaatt cagacaacac gttagaaagc gatggtgcag gcggtggtaa 3120 catttcagac ggcaaatcta ctaatggcgg cggtggtgga gctgatgata aatctaccat 3180 cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc 3240 agacggcggt ttaggctcaa atgtctcttt aggcaacaca gtcggcacct caactattgt 3300 actggtttcg ggcgatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc 3360 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca 3420 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg 3480 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat 3540 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga 3600 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 3660 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 3720 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 3780 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 3840 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 3900 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 3960 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 4020 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 4080 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 4140 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 4200 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 4260 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 4320 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 4380 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 4440 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 4500 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 4560 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 4620 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 4680 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 4740 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 4800 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 4860 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 4920 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 4980 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 5040 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 5100 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 5160 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 5220 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 5280 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 5340 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 5400 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 5460 acgaccgagc gcagcgagtc agtgagcgag gaagcatcct gcaccatcgt ctgctcatcc 5520 atgacctgac catgcagagg atgatgctcg tgacggttaa cgcctcgaat cagcaacggc 5580 ttgccgttca gcagcagcag accattttca atccgcacct cgcggaaacc gacatcgcag 5640 gcttctgctt caatcagcgt gccgtcggcg gtgtgcagtt caaccaccgc acgatagaga 5700 ttcgggattt cggcgctcca cagtttcggg ttttcgacgt tcagacgtag tgtgacgcga 5760 tcggcataac caccacgctc atcgataatt tcaccgccga aaggcgcggt gccgctggcg 5820 acctgcgttt caccctgcca taaagaaact gttacccgta ggtagtcacg caactcgccg 5880 cacatctgaa cttcagcctc cagtacagcg cggctgaaat catcattaaa gcgagtggca 5940 acatggaaat cgctgatttg tgtagtcggt ttatgcagca acgagacgtc acggaaaatg 6000 ccgctcatcc gccacatatc ctgatcttcc agataactgc cgtcactcca gcgcagcacc 6060 atcaccgcga ggcggttttc tccggcgcgt aaaaatgcgc tcaggtcaaa ttcagacggc 6120 aaacgactgt cctggccgta accgacccag cgcccgttgc accacagatg aaacgccgag 6180 ttaacgccat caaaaataat tcgcgtctgg ccttcctgta gccagctttc atcaacatta 6240 aatgtgagcg agtaacaacc cgtcggattc tccgtgggaa caaacggcgg attgaccgta 6300 atgggatagg tcacgttggt gtagatgggc gcatcgtaac cgtgcatctg ccagtttgag 6360 gggacgacga cagtatcggc ctcaggaaga tcgcactcca gccagctttc cggcaccgct 6420 tctggtgccg gaaaccaggc aaagcgccat tcgccattca ggctgcgcaa ctgttgggaa 6480 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca 6540 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggga 6600 tctatcattt ttagcagtga ttctaattgc agctgctctt tgatacaact aattttacga 6660 cgacgatgcg agcttttatt caaccgagcg tgcatgtttg caatcgtgca agcgttatca 6720 atttttcatt atcgtattgt tgcacatcaa caggctggac accacgttga actcgccgca 6780 gttttgcggc aagttggacc cgccgcgcat ccaatgcaaa ctttccgaca ttctgttgcc 6840 tacgaacgat tgattctttg tccattgatc gaagcgagtg ccttcgactt tttcgtgtcc 6900 agtgtggctt 6910 //