LOCUS       pBlueBacmsGCA1dexon6        6689 bp    DNA     circular     24-NOV-2015
DEFINITION  LMBP 4468.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4468
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 24-NOV-2015
            
COMMENT     VNTDATE|617114111|
COMMENT     VNTNAME|pBlueBacmsGCA1dexon6|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBlueBacmsGCA1dexon6" 0 6689 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 892940668 0 "" 0 "" "" "" "" "" "" 
COMMENT             "pBlueBachsGCA1His" "" 0 0 1 187 1 187 1 187 0 "" "" "" "" "" "" 
COMMENT             (CObList) (CTerm 4 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 187 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 6116508 0 "" 0 "" "" "" "" "" "" "msGCA1-exon6" "" 
COMMENT             0 0 1 1646 188 1833 1 1646 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 4 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 4 1 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #2=(CFragment 3 1 0 544501349 0 "" 0 "" "" "" "" "" "" 
COMMENT             "pBlueBachsGCA1His" "" 0 0 1 4856 1834 6689 2261 7116 0 "" "" "" "" 
COMMENT             "" "" (CObList) (CTerm 2 0 2261 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 4 1 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 1 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "EcoRV" "HaeII" "HindIII" "ScaI" "XbaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #3=(COligo "V5 epitope" "GGNAARCCNATHCCNAAYCCNYTNYTNGGNYTNGAYWSNACN" 
COMMENT             "length: 42 bp" 0 (CStringList "misc. marker") 0)) 1 "{(0,1),2}" 0 
COMMENT        0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 6225 6230 1 6689 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #4=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1847 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 4 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 6 609 0 2221 0 4915 0 5496 
COMMENT            0 5933 0 6227 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "ScaI" "agtact" 4 0 2 360 0 3786 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 174 0 0 "")) 
COMMENT       (CObList 
COMMENT        #9=(CFSignal (CObList) "AcNPV-PH promoter" 29 0 0 7 95 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "7..95") 
COMMENT        #10=(CFSignal (CObList) "msGucy1a3 fragment" 4 0 0 215 1804 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "215..1804") 
COMMENT        #11=(CFSignal (CObList) "ampicillin resistance" 4 0 0 3479 4339 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3479..4339") 
COMMENT        #12=(CFSignal (CObList) "His-tag" 22 0 0 1909 1926 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1909..1926") 
COMMENT        #13=(CFSignal (CObList) "pMB1 ori" 33 0 0 4880 5274 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4880..5274") 
COMMENT        #14=(CFSignal (CObList) "AcNPV-ETL promoter" 29 0 1 6388 6662 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(6388..6662)") 
COMMENT        #15=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1951 2025 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1951..2025") 
COMMENT        #16=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1951 2025 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1951..2025)") 
COMMENT        #17=(CFSignal (CObList) "V5 epitope" 22 0 0 1858 1899 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1858..1899") 
COMMENT        #18=(CFSignal (CObList) "lacZ fragment" 4 0 1 5275 6387 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "AcNPV ORF 1629 fragment" 4 0 1 2213 3091 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "")) 
COMMENT       (CObList 
COMMENT        #20=(CMSite "V5 epitope" "GGNAARCCNATHCCNAAYCCNYTNYTNGGNYTNGAYWSNACN" "" 
COMMENT             0)) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #21=(CFSignal (CObList) "LMBP 4468" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #22=(CFSignal (CObList) "Last modified: 24-NOV-2015" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #23=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #24=(CRefLinePar 
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COMMENT                    "pBlueBacmsGCA1dexon6" 2) 5 "" 0 4) 
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COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pBlueBacmsGCA1dexon6'" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4468." 
COMMENT                          1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 02/06/12 12:35" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 11/24/15 09:51" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6689 bp" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Constructed" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #34=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 6-FEB-2012" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #37=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 4 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "GeneCorner Plasmid Collection" 1))) "Author") 
COMMENT              #39=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #40=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #41=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #42=(CLinePar (CParagraph 0 (15 #21# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 4468") 
COMMENT                     #43=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #44=(CLinePar (CParagraph 0 (15 #22# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 24-NOV-2015"))) 
COMMENT                   "Annotations") 
COMMENT              #45=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 10 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #46=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (5 #0# 0) 1 2 1 0 195) 
COMMENT                           (CObjectList 
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COMMENT                                 "parent position: from 1 to 187" 1) 
COMMENT                            #48=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 187" 1) 
COMMENT                            #49=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 1 to 187" 1) 
COMMENT                            #50=(CFolderPar 
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COMMENT                                        "  Start of Molecule" 1))) 
COMMENT                                 "Left Terminus") 
COMMENT                            #52=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  Position 187" 1))) "Right Terminus"))) 
COMMENT                          "#1: FRAGMENT of <a href=\"vnti:dnarna/pBlueBachsGCA1His?sel=1-187\">pBlueBachsGCA1His</a> ") 
COMMENT                     #54=(CFolderPar 
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COMMENT                                 "parent position: from 1 to 1646" 1) 
COMMENT                            #56=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 1646" 1) 
COMMENT                            #57=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 188 to 1833" 1) 
COMMENT                            #58=(CFolderPar 
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COMMENT                                        "  Start of Molecule" 1))) 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  End of Molecule" 1))) 
COMMENT                                 "Right Terminus"))) 
COMMENT                          "#2: FRAGMENT of <a href=\"vnti:dnarna/msGCA1-exon6?sel=1-1646\">msGCA1-exon6</a> ") 
COMMENT                     #62=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (5 #2# 0) 1 2 1 0 195) 
COMMENT                           (CObjectList 
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COMMENT                                 "parent position: from 2261 to 7116" 1) 
COMMENT                            #64=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 4856" 1) 
COMMENT                            #65=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 1834 to 6689" 1) 
COMMENT                            #66=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  Position 2261" 1))) "Left Terminus") 
COMMENT                            #68=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  End of Molecule" 1))) 
COMMENT                                 "Right Terminus"))) 
COMMENT                          "#3: FRAGMENT of <a href=\"vnti:dnarna/pBlueBachsGCA1His?sel=2261-7116\">pBlueBachsGCA1His</a> "))) 
COMMENT                   "Component Fragments") 
COMMENT              #70=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #71=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #72=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 215 (3 #10# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 215   End: 1804" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   215..1804" 1))) "msGucy1a3 fragment") 
COMMENT                            #76=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2213 (3 #19# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2213  End: 3091 (Complementary)" 
COMMENT                                        1))) "AcNPV ORF 1629 fragment") 
COMMENT                            #78=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3479 (3 #11# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3479  End: 4339" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3479..4339" 1))) 
COMMENT                                 "ampicillin resistance") 
COMMENT                            #82=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5275 (3 #18# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5275  End: 6387 (Complementary)" 
COMMENT                                        1))) "lacZ fragment"))) "CDS (4 total)") 
COMMENT                     #84=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #85=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1858 (3 #17# 0) 1 2 2 0 194) 
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COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1858  End: 1899" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1858..1899" 1))) "V5 epitope") 
COMMENT                            #89=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1909 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1909  End: 1926" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1909..1926" 1))) "His-tag"))) 
COMMENT                          "Misc. Marker (2 total)") 
COMMENT                     #93=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #94=(CFolderPar 
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COMMENT                                        "Start: 7     End: 95  " 1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   7..95" 1))) "AcNPV-PH promoter") 
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COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6388 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6388  End: 6662 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   complement(6388..6662)" 1))) 
COMMENT                                 "AcNPV-ETL promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                            (CObjectList 
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COMMENT                                     #105=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #106=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   4880..5274" 1))) "pMB1 ori"))) 
COMMENT                           "Replication Origin (1 total)") 
COMMENT                     #107=(CFolderPar 
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COMMENT                            (CObjectList 
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COMMENT                                    (CObjectList 
COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1951  End: 2025" 1) 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #111=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1951..2025" 1))) 
COMMENT                                   "SV40 polyA early") 
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COMMENT                                    (CObjectList 
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COMMENT                                           "Start: 1951  End: 2025 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(1951..2025)" 1))) 
COMMENT                                   "SV40 polyA late"))) "Terminator (2 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                     (CObjectList 
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COMMENT                              (CObjectList 
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COMMENT                                            " N1: 1847 " 1))))) 
COMMENT                             "HindIII: 1 site") 1 5 "AAGCTT" "TTCGAA") 
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COMMENT                                            " N1: 4 " 1))))) "EcoRV: 1 site") 3 
COMMENT                            3 "GATATC" "CTATAG") 
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COMMENT                                            " N1: 174 " 1))))) "XbaI: 1 site") 1 
COMMENT                            5 "TCTAGA" "AGATCT") 
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COMMENT                                            " N1: 360 " 1) 
COMMENT                                      #129=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3786 " 1))))) "ScaI: 2 sites") 
COMMENT                            3 3 "AGTACT" "TCATGA") 
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COMMENT                                            " N1: 609 " 1) 
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COMMENT                                            " N2: 2221 " 1) 
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COMMENT                                            " N3: 4915 " 1) 
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COMMENT                                      #136=(CLinePar 
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COMMENT                                            " N5: 5933 " 1) 
COMMENT                                      #137=(CLinePar 
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COMMENT                                            " N6: 6227 " 1))))) "HaeII: 6 sites") 
COMMENT                            5 1 "RGCGCY" "YCGCGR"))) 
COMMENT                    "Restriction/Methylation Map") 
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COMMENT                            "   Similarity between ambiguous nucleotides (if any)" 
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COMMENT                                            " GGNAARCCNATHCCNAAYCCNYTNYTNGGNYTNGAYWSNACN" 
COMMENT                                            1)))))) "V5 epitope (0 sites)"))) 
COMMENT                    "Motifs (both strands)") 
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COMMENT                             " 3426: pBlueBacmsGCA1dexon6: ScaI(360) - ScaI(3786)" 
COMMENT                             1) 4 "" 360 3785) 
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COMMENT                             " 3263: pBlueBacmsGCA1dexon6: ScaI(3786) - ScaI(360)" 
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FEATURES             Location/Qualifiers
     promoter        7..95
                     /vntifkey="29"
                     /label=AcNPV-PH\promoter
     CDS             215..1804
                     /vntifkey="4"
                     /label=msGucy1a3\fragment
     CDS             3479..4339
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_marker     1909..1926
                     /vntifkey="22"
                     /label=His-tag
     rep_origin      4880..5274
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(6388..6662)
                     /vntifkey="29"
                     /label=AcNPV-ETL\promoter
     terminator      1951..2025
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(1951..2025)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     misc_marker     1858..1899
                     /vntifkey="22"
                     /label=V5\epitope
     CDS             complement(5275..6387)
                     /vntifkey="4"
                     /label=lacZ\fragment
     CDS             complement(2213..3091)
                     /vntifkey="4"
                     /label=AcNPV\ORF\1629\fragment
BASE COUNT     1690 a      1639 c      1645 g      1715 t 
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt 
       61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca 
      121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat 
      181 tgcccttaag ccactgccaa gctccaggaa caccatgttc tgcaggaagt tcaaggatct 
      241 caagatcaca ggggagtgtc ctttctcctt actggcccct ggtcaggttc ctaaggagcc 
      301 aacagaggag gtggctggag gctctgaggg ctgccaggct actctgccca tctgccagta 
      361 ctttcctgag aagaatgcag aagggagtct cccccaaaga aagacaagcc gcaacagagt 
      421 ctacctgcac accctggcag agagtatttg caagctcatc ttcccagagt gtgagcgact 
      481 gaaccttgca cttcagagaa ccttggcaaa gcataaaata gaagaaaaca ggaaatcttc 
      541 agaaaaagaa gaccttgaaa aaataatcgc agaagaagca attgcagcag gtgccccagt 
      601 ggaggcgctc aaagactctc tgggcgagga gctgttcaag atctgctatg aggaagatga 
      661 gcacattttg ggcgtggttg gcggcaccct gaaggacttt ctaaatagct tcagcacgct 
      721 cctcaagcag agcagccact gccaagaggc ggagaggcgg ggacgactgg aagatgcctc 
      781 catcttatgc ctggacaagg accaggactt tctaaatgtt tactacttct tcccgaagag 
      841 aaccacagcc ctgcttctcc ctggtatcat taaagcggct gctcgcatac tgtacgaaag 
      901 ccacgtggag gtgtccctga tgcctccctg cttccgaagt gactgtaccg agtttgtgaa 
      961 ccagccctat ttgctctact ccgttcatgt gaagagcacc aagccgtccc tgtccccagg 
     1021 caagccccag tcctcgctag tgatccccgc ttcgctcttc tgcaagactt tcccgttcca 
     1081 tttcatgctg gaccgagacc tggccatcct gcagctgggt aacggcatca gaaggctggt 
     1141 gaacaagagg gacttccaag ggaagcccaa ctttgaagag ttctttgaaa ttctaactcc 
     1201 caaaatcaac cagacattta gtggcatcat gacaatgttg aacatgcagt ttgtcatccg 
     1261 ggtgaggaga tgggataact cggtgaagaa atcgtcaagg gtggagacca tcggggatgc 
     1321 atattgtgtg gcaggtggat tgcacagaga gagtgacacc catgctgtcc agatagcact 
     1381 gatggccctg aagatgatgg agctctccaa tgaggtcatg tctccccacg gagaacctat 
     1441 caagatgcga attggactac attctggatc agtgtttgct ggagttgtcg gagtgaagat 
     1501 gccccggtat tgcctgtttg gaaacaatgt cactctggct aacaaatttg aatcctgcag 
     1561 tgtgcctcgg aaaatcaatg tcagccccac cacatacagg ttactcaaag actgtcctgg 
     1621 ctttgtgttc accccgagat caagggagga gcttccacca aacttcccta gtgacattcc 
     1681 tgggatctgt cactttctgg atgcttatca ccatcaagga cctaattcca aaccatggtt 
     1741 ccaggataaa gatgtggaag atggaaacgc caacttctta ggcaaagcgt caggggtaga 
     1801 ttagtgagcc acatgctctt atgtttgatg cctaagggca attcgaagct taggcctggt 
     1861 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat 
     1921 caccattgac gtctctagct tggagtcgac aacttgttta ttgcagctta taatggttac 
     1981 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 
     2041 tgtggtttgt ccaaactcat caatgtatct tatcatctct cgactctgct gaagaggagg 
     2101 aaattctcct tgaagtttcc ctggtgttca aagtaaagga gtttgcacca gacgcacctc 
     2161 tgttcactgg tccggcgtat taaaacacga tacattgtta ttagtacatt tattaagcgc 
     2221 tagattctgt gcgttgttga tttacagaca attgttgtac gtattttaat aattcattaa 
     2281 atttataatc tttagggtgg tatgttagag cgaaaatcaa atgattttca gcgtctttat 
     2341 atctgaattt aaatattaaa tcctcaatag atttgtaaaa taggtttcga ttagtttcaa 
     2401 acaagggttg tttttccgaa ccgatggctg gactatctaa tggattttcg ctcaacgcca 
     2461 caaaacttgc caaatcttgt agcagcaatc tagctttgtc gatattcgtt tgtgttttgt 
     2521 tttgtaataa aggttcgacg tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac 
     2581 tgtcgttagt gtacaattga ctcgacgtaa acacgttaaa taaagcctgg acatatttaa 
     2641 catcgggcgt gttagcttta ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag 
     2701 aagttgcttc cgaagacgat tttgccatag ccacacgacg cctattaatt gtgtcggcta 
     2761 acacgtccgc gatcaaattt gtagttgagc tttttggaat tatttctgat tgcgggcgtt 
     2821 tttgggcggg tttcaatcta actgtgcccg attttaattc agacaacacg ttagaaagcg 
     2881 atggtgcagg cggtggtaac atttcagacg gcaaatctac taatggcggc ggtggtggag 
     2941 ctgatgataa atctaccatc ggtggaggcg caggcggggc tggcggcgga ggcggaggcg 
     3001 gaggtggtgg cggtgatgca gacggcggtt taggctcaaa tgtctcttta ggcaacacag 
     3061 tcggcacctc aactattgta ctggtttcgg gcgatggtgc actctcagta caatctgctc 
     3121 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg 
     3181 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat 
     3241 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg 
     3301 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt 
     3361 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 
     3421 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 
     3481 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 
     3541 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 
     3601 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 
     3661 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 
     3721 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 
     3781 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 
     3841 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 
     3901 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 
     3961 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 
     4021 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 
     4081 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 
     4141 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 
     4201 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 
     4261 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 
     4321 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 
     4381 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 
     4441 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 
     4501 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 
     4561 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 
     4621 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 
     4681 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 
     4741 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 
     4801 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 
     4861 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 
     4921 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 
     4981 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 
     5041 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 
     5101 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 
     5161 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 
     5221 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcatcctg 
     5281 caccatcgtc tgctcatcca tgacctgacc atgcagagga tgatgctcgt gacggttaac 
     5341 gcctcgaatc agcaacggct tgccgttcag cagcagcaga ccattttcaa tccgcacctc 
     5401 gcggaaaccg acatcgcagg cttctgcttc aatcagcgtg ccgtcggcgg tgtgcagttc 
     5461 aaccaccgca cgatagagat tcgggatttc ggcgctccac agtttcgggt tttcgacgtt 
     5521 cagacgtagt gtgacgcgat cggcataacc accacgctca tcgataattt caccgccgaa 
     5581 aggcgcggtg ccgctggcga cctgcgtttc accctgccat aaagaaactg ttacccgtag 
     5641 gtagtcacgc aactcgccgc acatctgaac ttcagcctcc agtacagcgc ggctgaaatc 
     5701 atcattaaag cgagtggcaa catggaaatc gctgatttgt gtagtcggtt tatgcagcaa 
     5761 cgagacgtca cggaaaatgc cgctcatccg ccacatatcc tgatcttcca gataactgcc 
     5821 gtcactccag cgcagcacca tcaccgcgag gcggttttct ccggcgcgta aaaatgcgct 
     5881 caggtcaaat tcagacggca aacgactgtc ctggccgtaa ccgacccagc gcccgttgca 
     5941 ccacagatga aacgccgagt taacgccatc aaaaataatt cgcgtctggc cttcctgtag 
     6001 ccagctttca tcaacattaa atgtgagcga gtaacaaccc gtcggattct ccgtgggaac 
     6061 aaacggcgga ttgaccgtaa tgggataggt cacgttggtg tagatgggcg catcgtaacc 
     6121 gtgcatctgc cagtttgagg ggacgacgac agtatcggcc tcaggaagat cgcactccag 
     6181 ccagctttcc ggcaccgctt ctggtgccgg aaaccaggca aagcgccatt cgccattcag 
     6241 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 
     6301 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 
     6361 acgttgtaaa acgacgggat ctatcatttt tagcagtgat tctaattgca gctgctcttt 
     6421 gatacaacta attttacgac gacgatgcga gcttttattc aaccgagcgt gcatgtttgc 
     6481 aatcgtgcaa gcgttatcaa tttttcatta tcgtattgtt gcacatcaac aggctggaca 
     6541 ccacgttgaa ctcgccgcag ttttgcggca agttggaccc gccgcgcatc caatgcaaac 
     6601 tttccgacat tctgttgcct acgaacgatt gattctttgt ccattgatcg aagcgagtgc 
     6661 cttcgacttt ttcgtgtcca gtgtggctt 
//
