ID pBlueBacmsGCA1His standard; circular DNA; ; 7143 BP. DE LMBP 4470. CC Accession number: LMBP 4470 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 11-DEC-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 7..95 FT /vntifkey="29" FT /label=AcNPV-PH\promoter FT /note="AcNPV polyhedrin promoter" FT CDS 215..2287 FT /vntifkey="4" FT /label=msGCA1 FT /note="mouse soluble guanylate cyclase alpha1 coding sequence" FT misc_marker 2312..2353 FT /vntifkey="22" FT /label=V5\epitope FT /note="V5 epitope" FT misc_marker 2363..2380 FT /vntifkey="22" FT /label=His-tag FT /note="polyhistidine (6 x His) tag" FT terminator 2405..2479 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(2405..2479) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT CDS complement(2667..3545) FT /vntifkey="4" FT /label=3'\AcNPV\ORF\1629 FT /note="3' fragment of the AcNPV open reading frame 1629" FT CDS 3933..4793 FT /vntifkey="4" FT /label=ampicillin FT rep_origin 5334..5728 FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(5729..6841) FT /vntifkey="4" FT /label=5'\lacZ\fragment FT /note="5' fragment of the E. coli lacZ coding sequence" FT promoter complement(6842..7116) FT /vntifkey="29" FT /label=AcNPV-ETL\promoter FT /note="AcNPV EcoRI-T large promoter" SQ Sequence 7143 BP; 1802 A; 1749 C; 1772 G; 1820 t; gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt 60 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca 120 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat 180 tgcccttaag ccactgccaa gctccaggaa caccatgttc tgcaggaagt tcaaggatct 240 caagatcaca ggggagtgtc ctttctcctt actggcccct ggtcaggttc ctaaggagcc 300 aacagaggag gtggctggag gctctgaggg ctgccaggct actctgccca tctgccagta 360 ctttcctgag aagaatgcag aagggagtct cccccaaaga aagacaagcc gcaacagagt 420 ctacctgcac accctggcag agagtatttg caagctcatc ttcccagagt gtgagcgact 480 gaaccttgca cttcagagaa ccttggcaaa gcataaaata gaagaaaaca ggaaatcttc 540 agaaaaagaa gaccttgaaa aaataatcgc agaagaagca attgcagcag gtgccccagt 600 ggaggcgctc aaagactctc tgggcgagga gctgttcaag atctgctatg aggaagatga 660 gcacattttg ggcgtggttg gcggcaccct gaaggacttt ctaaatagct tcagcacgct 720 cctcaagcag agcagccact gccaagaggc ggagaggcgg ggacgactgg aagatgcctc 780 catcttatgc ctggacaagg accaggactt tctaaatgtt tactacttct tcccgaagag 840 aaccacagcc ctgcttctcc ctggtatcat taaagcggct gctcgcatac tgtacgaaag 900 ccacgtggag gtgtccctga tgcctccctg cttccgaagt gactgtaccg agtttgtgaa 960 ccagccctat ttgctctact ccgttcatgt gaagagcacc aagccgtccc tgtccccagg 1020 caagccccag tcctcgctag tgatccccgc ttcgctcttc tgcaagactt tcccgttcca 1080 tttcatgctg gaccgagacc tggccatcct gcagctgggt aacggcatca gaaggctggt 1140 gaacaagagg gacttccaag ggaagcccaa ctttgaagag ttctttgaaa ttctaactcc 1200 caaaatcaac cagacattta gtggcatcat gacaatgttg aacatgcagt ttgtcatccg 1260 ggtgaggaga tgggataact cggtgaagaa atcgtcaagg gttatggatc tcaaaggtca 1320 aatgatctac atcgttgaat ccagtgccat cttgttctta gggtcaccat gtgtggacag 1380 gctggaagat ttcacaggac gggggctcta tctgtccgac atcccaattc ataacgccct 1440 gagggatgtt gtcttgatag gggagcaggc acgggctcaa gatggcctca agaagaggtt 1500 ggggaagctg aaggcaaccc tggagcatgc ccaccaagcc ctggaggaag agaagaagag 1560 gacagtggat ctgctgtgct ctatcttccc ctctgaggtt gctcagcagc tgtggcaagg 1620 acaaattgtg caagccaaga aattcagcga ggtcaccatg cttttctcag atatcgtagg 1680 gttcactgct atctgctctc agtgttcacc tctgcaggtc atcacgatgc tcaacgctct 1740 ctacactcgc tttgaccagc agtgtggaga gctggatgtc tacaaggtgg agaccatcgg 1800 ggatgcatat tgtgtggcag gtggattgca cagagagagt gacacccatg ctgtccagat 1860 agcactgatg gccctgaaga tgatggagct ctccaatgag gtcatgtctc cccacggaga 1920 acctatcaag atgcgaattg gactacattc tggatcagtg tttgctggag ttgtcggagt 1980 gaagatgccc cggtattgcc tgtttggaaa caatgtcact ctggctaaca aatttgaatc 2040 ctgcagtgtg cctcggaaaa tcaatgtcag ccccaccaca tacaggttac tcaaagactg 2100 tcctggcttt gtgttcaccc cgagatcaag ggaggagctt ccaccaaact tccctagtga 2160 cattcctggg atctgtcact ttctggatgc ttatcaccat caaggaccta attccaaacc 2220 atggttccag gataaagatg tggaagatgg aaacgccaac ttcttaggca aagcgtcagg 2280 ggtagataag ggcaattcga agcttaggcc tggtaagcct atccctaacc ctctcctcgg 2340 tctcgattct acgcgtaccg gtcatcatca ccatcaccat tgacgtctct agcttggagt 2400 cgacaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 2460 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 2520 atcttatcat ctctcgactc tgctgaagag gaggaaattc tccttgaagt ttccctggtg 2580 ttcaaagtaa aggagtttgc accagacgca cctctgttca ctggtccggc gtattaaaac 2640 acgatacatt gttattagta catttattaa gcgctagatt ctgtgcgttg ttgatttaca 2700 gacaattgtt gtacgtattt taataattca ttaaatttat aatctttagg gtggtatgtt 2760 agagcgaaaa tcaaatgatt ttcagcgtct ttatatctga atttaaatat taaatcctca 2820 atagatttgt aaaataggtt tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg 2880 gctggactat ctaatggatt ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc 2940 aatctagctt tgtcgatatt cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc 3000 aaaatattat gcgcttttgt atttctttca tcactgtcgt tagtgtacaa ttgactcgac 3060 gtaaacacgt taaataaagc ctggacatat ttaacatcgg gcgtgttagc tttattaggc 3120 cgattatcgt cgtcgtccca accctcgtcg ttagaagttg cttccgaaga cgattttgcc 3180 atagccacac gacgcctatt aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt 3240 gagctttttg gaattatttc tgattgcggg cgtttttggg cgggtttcaa tctaactgtg 3300 cccgatttta attcagacaa cacgttagaa agcgatggtg caggcggtgg taacatttca 3360 gacggcaaat ctactaatgg cggcggtggt ggagctgatg ataaatctac catcggtgga 3420 ggcgcaggcg gggctggcgg cggaggcgga ggcggaggtg gtggcggtga tgcagacggc 3480 ggtttaggct caaatgtctc tttaggcaac acagtcggca cctcaactat tgtactggtt 3540 tcgggcgatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc 3600 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 3660 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 3720 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga 3780 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 3840 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 3900 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 3960 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 4020 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 4080 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 4140 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 4200 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 4260 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 4320 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 4380 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 4440 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 4500 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 4560 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 4620 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 4680 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 4740 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 4800 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 4860 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 4920 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 4980 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 5040 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 5100 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 5160 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 5220 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 5280 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 5340 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 5400 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 5460 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 5520 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 5580 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 5640 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 5700 agcgcagcga gtcagtgagc gaggaagcat cctgcaccat cgtctgctca tccatgacct 5760 gaccatgcag aggatgatgc tcgtgacggt taacgcctcg aatcagcaac ggcttgccgt 5820 tcagcagcag cagaccattt tcaatccgca cctcgcggaa accgacatcg caggcttctg 5880 cttcaatcag cgtgccgtcg gcggtgtgca gttcaaccac cgcacgatag agattcggga 5940 tttcggcgct ccacagtttc gggttttcga cgttcagacg tagtgtgacg cgatcggcat 6000 aaccaccacg ctcatcgata atttcaccgc cgaaaggcgc ggtgccgctg gcgacctgcg 6060 tttcaccctg ccataaagaa actgttaccc gtaggtagtc acgcaactcg ccgcacatct 6120 gaacttcagc ctccagtaca gcgcggctga aatcatcatt aaagcgagtg gcaacatgga 6180 aatcgctgat ttgtgtagtc ggtttatgca gcaacgagac gtcacggaaa atgccgctca 6240 tccgccacat atcctgatct tccagataac tgccgtcact ccagcgcagc accatcaccg 6300 cgaggcggtt ttctccggcg cgtaaaaatg cgctcaggtc aaattcagac ggcaaacgac 6360 tgtcctggcc gtaaccgacc cagcgcccgt tgcaccacag atgaaacgcc gagttaacgc 6420 catcaaaaat aattcgcgtc tggccttcct gtagccagct ttcatcaaca ttaaatgtga 6480 gcgagtaaca acccgtcgga ttctccgtgg gaacaaacgg cggattgacc gtaatgggat 6540 aggtcacgtt ggtgtagatg ggcgcatcgt aaccgtgcat ctgccagttt gaggggacga 6600 cgacagtatc ggcctcagga agatcgcact ccagccagct ttccggcacc gcttctggtg 6660 ccggaaacca ggcaaagcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 6720 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 6780 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg ggatctatca 6840 tttttagcag tgattctaat tgcagctgct ctttgataca actaatttta cgacgacgat 6900 gcgagctttt attcaaccga gcgtgcatgt ttgcaatcgt gcaagcgtta tcaatttttc 6960 attatcgtat tgttgcacat caacaggctg gacaccacgt tgaactcgcc gcagttttgc 7020 ggcaagttgg acccgccgcg catccaatgc aaactttccg acattctgtt gcctacgaac 7080 gattgattct ttgtccattg atcgaagcga gtgccttcga ctttttcgtg tccagtgtgg 7140 ctt 7143 //