ID pBlueBacmsGCA1 standard; circular DNA; ; 7173 BP. DE LMBP 4471. CC Accession number: LMBP 4471 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 11-DEC-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 7..95 FT /vntifkey="29" FT /label=AcNPV-PH\promoter FT /note="AcNPV polyhedrin promoter" FT CDS 213..2288 FT /vntifkey="4" FT /label=msGCA1 FT /note="mouse soluble guanylate cyclase alpha1 coding sequence" FT terminator 2435..2509 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(2435..2509) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT CDS complement(2697..3575) FT /vntifkey="4" FT /label=3'\AcNPV\ORF\1629 FT /note="3' fragment of the AcNPV open reading frame 1629" FT CDS 3963..4823 FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT rep_origin 5364..5758 FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(5759..6871) FT /vntifkey="4" FT /label=5'\lacZ\fragment FT /note="5' fragment of the E. coli lacZ coding sequence" FT promoter complement(6872..7146) FT /vntifkey="29" FT /label=AcNPV-ETL\promoter FT /note="AcNPV EcoRI-T large promoter" FT misc_marker 2342..2383 FT /vntifkey="22" FT /label=V5\epitope FT misc_marker 2393..2410 FT /vntifkey="22" FT /label=His-tag SQ Sequence 7173 BP; 1806 A; 1756 C; 1779 G; 1832 t; gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt 60 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca 120 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat 180 tgcccttgcc actgccaagc tccaggaaca ccatgttctg caggaagttc aaggatctca 240 agatcacagg ggagtgtcct ttctccttac tggcccctgg tcaggttcct aaggagccaa 300 cagaggaggt ggctggaggc tctgagggct gccaggctac tctgcccatc tgccagtact 360 ttcctgagaa gaatgcagaa gggagtctcc cccaaagaaa gacaagccgc aacagagtct 420 acctgcacac cctggcagag agtatttgca agctcatctt cccagagtgt gagcgactga 480 accttgcact tcagagaacc ttggcaaagc ataaaataga agaaaacagg aaatcttcag 540 aaaaagaaga ccttgaaaaa ataatcgcag aagaagcaat tgcagcaggt gccccagtgg 600 aggcgctcaa agactctctg ggcgaggagc tgttcaagat ctgctatgag gaagatgagc 660 acattttggg cgtggttggc ggcaccctga aggactttct aaatagcttc agcacgctcc 720 tcaagcagag cagccactgc caagaggcgg agaggcgggg acgactggaa gatgcctcca 780 tcttatgcct ggacaaggac caggactttc taaatgttta ctacttcttc ccgaagagaa 840 ccacagccct gcttctccct ggtatcatta aagcggctgc tcgcatactg tacgaaagcc 900 acgtggaggt gtccctgatg cctccctgct tccgaagtga ctgtaccgag tttgtgaacc 960 agccctattt gctctactcc gttcatgtga agagcaccaa gccgtccctg tccccaggca 1020 agccccagtc ctcgctagtg atccccgctt cgctcttctg caagactttc ccgttccatt 1080 tcatgctgga ccgagacctg gccatcctgc agctgggtaa cggcatcaga aggctggtga 1140 acaagaggga cttccaaggg aagcccaact ttgaagagtt ctttgaaatt ctaactccca 1200 aaatcaacca gacatttagt ggcatcatga caatgttgaa catgcagttt gtcatccggg 1260 tgaggagatg ggataactcg gtgaagaaat cgtcaagggt tatggatctc aaaggtcaaa 1320 tgatctacat cgttgaatcc agtgccatct tgttcttagg gtcaccatgt gtggacaggc 1380 tggaagattt cacaggacgg gggctctatc tgtccgacat cccaattcat aacgccctga 1440 gggatgttgt cttgataggg gagcaggcac gggctcaaga tggcctcaag aagaggttgg 1500 ggaagctgaa ggcaaccctg gagcatgccc accaagccct ggaggaagag aagaagagga 1560 cagtggatct gctgtgctct atcttcccct ctgaggttgc tcagcagctg tggcaaggac 1620 aaattgtgca agccaagaaa ttcagcgagg tcaccatgct tttctcagat atcgtagggt 1680 tcactgctat ctgctctcag tgttcacctc tgcaggtcat cacgatgctc aacgctctct 1740 acactcgctt tgaccagcag tgtggagagc tggatgtcta caaggtggag accatcgggg 1800 atgcatattg tgtggcaggt ggattgcaca gagagagtga cacccatgct gtccagatag 1860 cactgatggc cctgaagatg atggagctct ccaatgaggt catgtctccc cacggagaac 1920 ctatcaagat gcgaattgga ctacattctg gatcagtgtt tgctggagtt gtcggagtga 1980 agatgccccg gtattgcctg tttggaaaca atgtcactct ggctaacaaa tttgaatcct 2040 gcagtgtgcc tcggaaaatc aatgtcagcc ccaccacata caggttactc aaagactgtc 2100 ctggctttgt gttcaccccg agatcaaggg aggagcttcc accaaacttc cctagtgaca 2160 ttcctgggat ctgtcacttt ctggatgctt atcaccatca aggacctaat tccaaaccat 2220 ggttccagga taaagatgtg gaagatggaa acgccaactt cttaggcaaa gcgtcagggg 2280 tagattagtg agccacatgc tcttatgttt gatgcctaag ggcaattcga agcttaggcc 2340 tggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca 2400 ccatcaccat tgacgtctct agcttggagt cgacaacttg tttattgcag cttataatgg 2460 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 2520 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat ctctcgactc tgctgaagag 2580 gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa aggagtttgc accagacgca 2640 cctctgttca ctggtccggc gtattaaaac acgatacatt gttattagta catttattaa 2700 gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt taataattca 2760 ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt ttcagcgtct 2820 ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt tcgattagtt 2880 tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt ttcgctcaac 2940 gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt cgtttgtgtt 3000 ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt atttctttca 3060 tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaataaagc ctggacatat 3120 ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca accctcgtcg 3180 ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt aattgtgtcg 3240 gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattatttc tgattgcggg 3300 cgtttttggg cgggtttcaa tctaactgtg cccgatttta attcagacaa cacgttagaa 3360 agcgatggtg caggcggtgg taacatttca gacggcaaat ctactaatgg cggcggtggt 3420 ggagctgatg ataaatctac catcggtgga ggcgcaggcg gggctggcgg cggaggcgga 3480 ggcggaggtg gtggcggtga tgcagacggc ggtttaggct caaatgtctc tttaggcaac 3540 acagtcggca cctcaactat tgtactggtt tcgggcgatg gtgcactctc agtacaatct 3600 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct 3660 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 3720 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga 3780 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 3840 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 3900 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 3960 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 4020 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 4080 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 4140 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 4200 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 4260 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 4320 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 4380 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 4440 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 4500 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 4560 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 4620 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 4680 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 4740 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 4800 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 4860 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 4920 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 4980 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 5040 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 5100 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 5160 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 5220 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 5280 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 5340 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 5400 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 5460 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 5520 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 5580 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 5640 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 5700 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcat 5760 cctgcaccat cgtctgctca tccatgacct gaccatgcag aggatgatgc tcgtgacggt 5820 taacgcctcg aatcagcaac ggcttgccgt tcagcagcag cagaccattt tcaatccgca 5880 cctcgcggaa accgacatcg caggcttctg cttcaatcag cgtgccgtcg gcggtgtgca 5940 gttcaaccac cgcacgatag agattcggga tttcggcgct ccacagtttc gggttttcga 6000 cgttcagacg tagtgtgacg cgatcggcat aaccaccacg ctcatcgata atttcaccgc 6060 cgaaaggcgc ggtgccgctg gcgacctgcg tttcaccctg ccataaagaa actgttaccc 6120 gtaggtagtc acgcaactcg ccgcacatct gaacttcagc ctccagtaca gcgcggctga 6180 aatcatcatt aaagcgagtg gcaacatgga aatcgctgat ttgtgtagtc ggtttatgca 6240 gcaacgagac gtcacggaaa atgccgctca tccgccacat atcctgatct tccagataac 6300 tgccgtcact ccagcgcagc accatcaccg cgaggcggtt ttctccggcg cgtaaaaatg 6360 cgctcaggtc aaattcagac ggcaaacgac tgtcctggcc gtaaccgacc cagcgcccgt 6420 tgcaccacag atgaaacgcc gagttaacgc catcaaaaat aattcgcgtc tggccttcct 6480 gtagccagct ttcatcaaca ttaaatgtga gcgagtaaca acccgtcgga ttctccgtgg 6540 gaacaaacgg cggattgacc gtaatgggat aggtcacgtt ggtgtagatg ggcgcatcgt 6600 aaccgtgcat ctgccagttt gaggggacga cgacagtatc ggcctcagga agatcgcact 6660 ccagccagct ttccggcacc gcttctggtg ccggaaacca ggcaaagcgc cattcgccat 6720 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 6780 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 6840 cacgacgttg taaaacgacg ggatctatca tttttagcag tgattctaat tgcagctgct 6900 ctttgataca actaatttta cgacgacgat gcgagctttt attcaaccga gcgtgcatgt 6960 ttgcaatcgt gcaagcgtta tcaatttttc attatcgtat tgttgcacat caacaggctg 7020 gacaccacgt tgaactcgcc gcagttttgc ggcaagttgg acccgccgcg catccaatgc 7080 aaactttccg acattctgtt gcctacgaac gattgattct ttgtccattg atcgaagcga 7140 gtgccttcga ctttttcgtg tccagtgtgg ctt 7173 //