LOCUS       pGBF-Kozakcre           5006 bp    DNA     circular     22-OCT-2015
DEFINITION  LMBP 4474.
SOURCE      
  ORGANISM  
COMMENT     Accession number: 4474
            
COMMENT     VNTNAME|pGBF-Kozakcre|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pGBF-Kozakcre" 0 5006 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 1 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BamHI" "BglI" "HaeII" "NheI" "SpeI" "XmnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 1 5006 1 5006 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 973 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 1 3876 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 5 6 0 513 0 2092 0 2340 0 
COMMENT            4642 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 1339 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SpeI" "actagt" 2 0 2 263 0 296 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 2 1124 0 3394 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "lac promoter" 30 0 0 95 178 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "95..178") 
COMMENT        #7=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 0 179 216 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "179..216") 
COMMENT        #8=(CFSignal (CObList) "T3 promoter" 30 0 0 242 258 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "242..258") 
COMMENT        #9=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 2208 3002 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2208..3002") 
COMMENT        #10=(CFSignal (CObList) "ampicillin resistance" 4 0 0 3206 4066 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3206..4066") 
COMMENT        #11=(CFSignal (CObList) "T7 promoter" 30 0 1 1380 1396 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1380..1396)") 
COMMENT        #12=(CFSignal (CObList) "cre" 4 0 1 301 1332 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(301..1332)") 
COMMENT        #13=(CFSignal (CObList) "pMB1 ori" 33 0 0 4607 3 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4607..3")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #14=(CFSignal (CObList) "Last modified: 24-AUG-2012" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #15=(CFSignal (CObList) "Accession N\260.: 4474" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                   5 "" 0 4) 
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COMMENT                    (CObjectList 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pGBF-Kozakcre'" 1) 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4474." 
COMMENT                          1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 08/24/12 11:36" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5006 bp" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 24-AUG-2012" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "Last modified: 24-AUG-2012") 
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COMMENT                                 "" 1))) "Accession N\260.: 4474"))) 
COMMENT                   "Annotations") 
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COMMENT                                        "Start: 301   End: 1332 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #40=(CLinePar 
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COMMENT                                        "ORF2, put. cre protein (aa 1-343)" 1) 
COMMENT                                   #41=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #42=(CLinePar 
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COMMENT                                        "   complement(301..1332)" 1))) "cre") 
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COMMENT                                        "Start: 2208  End: 3002" 1) 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2208..3002" 1))) 
COMMENT                                 "Tn5 neomycin resistance") 
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COMMENT                                        "Start: 3206  End: 4066" 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3206..4066" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (3 total)") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 95    End: 178 " 1) 
COMMENT                                   #54=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   95..178" 1))) "lac promoter") 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #59=(CLinePar 
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COMMENT                                        "   242..258" 1))) "T3 promoter") 
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COMMENT                                        "Start: 1380  End: 1396 (Complementary)" 
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COMMENT                                   #62=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1380..1396)" 1))) 
COMMENT                                 "T7 promoter"))) 
COMMENT                          "Promoter Prokaryotic (3 total)") 
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COMMENT                                        "Start: 179   End: 216 " 1) 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   179..216" 1))) 
COMMENT                                 "5' UTR of lacZ, incl. RBS"))) "RBS (1 total)") 
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COMMENT                                        "Start: 4607  End: 3   " 1) 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4607..3" 1))) "pMB1 ori"))) 
COMMENT                          "Replication Origin (1 total)"))) "Feature Map") 
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COMMENT                                        " N1: 973 " 1))))) "BamHI: 1 site") 1 5 
COMMENT                          "GGATCC" "CCTAGG") 
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COMMENT                                        " N1: 3876 " 1))))) "BglI: 1 site") 7 4 
COMMENT                          "GCCNNNNNGGC" "CGGNNNNNCCG") 
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COMMENT                                        " N1: 1339 " 1))))) "NheI: 1 site") 1 5 
COMMENT                          "GCTAGC" "CGATCG") 
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COMMENT                                        " N1: 263 " 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 296 " 1))))) "SpeI: 2 sites") 1 5 
COMMENT                          "ACTAGT" "TGATCA") 
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COMMENT                                        " N1: 1124 " 1) 
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COMMENT                                        " N2: 3394 " 1))))) "XmnI: 2 sites") 5 5 
COMMENT                          "GAANNNNTTC" "CTTNNNNAAG") 
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COMMENT                                        " N1: 6 " 1) 
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COMMENT                                        " N2: 513 " 1) 
COMMENT                                   #96=(CLinePar 
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COMMENT                                        " N3: 2092 " 1) 
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COMMENT                                        " N4: 2340 " 1) 
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COMMENT                                        " N5: 4642 " 1))))) "HaeII: 5 sites") 5 
COMMENT                          1 "RGCGCY" "YCGCGR"))) "Restriction/Methylation Map") 
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COMMENT                           "   Similarity between ambiguous nucleotides (if any)" 
COMMENT                           1) 
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COMMENT                           "is calculated as an AVERAGE similarity" 1))) 
COMMENT                   "Motifs (both strands)") 
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COMMENT                             " 248: pGBF-Kozakcre: HaeII(2092) - HaeII(2340)" 1) 
COMMENT                            4 "" 2092 2339))) "Restriction Fragments")))) 
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COMMENT        #229=(CSampFrag "pGBF-Kozakcre" 2302 2340 "HaeII" 4642 "HaeII" 
COMMENT              (LOGPEN 0 0 0) 6.46578e+170 6.02986e-310) 
COMMENT        #230=(CSampFrag "pGBF-Kozakcre" 1579 513 "HaeII" 2092 "HaeII" 
COMMENT              (LOGPEN 0 0 0) 1.40375e-316 7.04381e-308) 
COMMENT        #231=(CSampFrag "pGBF-Kozakcre" 507 6 "HaeII" 513 "HaeII" (LOGPEN 0 0 0) 
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FEATURES             Location/Qualifiers
     promoter        95..178
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             179..216
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        242..258
                     /vntifkey="30"
                     /label=T3\promoter
     CDS             2208..3002
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     CDS             3206..4066
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        complement(1380..1396)
                     /vntifkey="30"
                     /label=T7\promoter
     CDS             complement(301..1332)
                     /vntifkey="4"
                     /label=cre
                     /note="ORF2, put. cre protein (aa 1-343)"
     rep_origin      4607..3
                     /vntifkey="33"
                     /label=pMB1\ori
BASE COUNT     1205 a      1282 c      1302 g      1217 t 
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca 
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc ccttactagt 
      301 ctaatcgcca tcttccagca ggcgcaccat tgcccctgtt tcactatcca ggttacggat 
      361 atagttcatg acaatattta cattggtcca gccaccagct tgcatgatct ccggtattga 
      421 aactccagcg cgggccatat ctcgcgcggc tccgacacgg gcactgtgtc cagaccaggc 
      481 caggtatctc tgaccagagt catccttagc gccgtaaatc aatcgatgag ttgcttcaaa 
      541 aatcccttcc agggcgcgag ttgatagctg gctggtggca gatggcgcgg caacaccatt 
      601 ttttctgacc cggcaaaaca ggtagttatt cggatcatca gctacaccag agacggaaat 
      661 ccatcgctcg accagtttag ttacccccag gctaagtgcc ttctctacac ctgcggtgct 
      721 aaccagcgtt ttcgttctgc caatatggat taacattctc ccaccgtcag tacgtgagat 
      781 atctttaacc ctgatcctgg caatttcggc tatacgtaac agggtgttat aagcaatccc 
      841 cagaaatgcc agattacgta tatcctggca gcgatcgcta ttttccatga gtgaacgaac 
      901 ctggtcgaaa tcagtgcgtt cgaacgctag agcctgtttt gcacgttcac cggcatcaac 
      961 gttttctttt cggatccgcc gcataaccag tgaaacagca ttgctgtcac ttggtcgtgg 
     1021 cagcccggac cgacgatgaa gcatgtttag ctggcccaaa tgttgctgga tagtttttac 
     1081 tgccagaccg cgcgcctgaa gatatagaag ataatcgcga acatcttcag gttctgcggg 
     1141 aaaccatttc cggttattca acttgcacca tgccgcccac gaccggcaaa cggacagaag 
     1201 cattttccag gtatgctcag aaaacgcctg gcgatccctg aacatgtcca tcaggttctt 
     1261 gcgaacctca tcactcgttg catcgaccgg taatgcaggc aaattttggt gtacggtcag 
     1321 taaattggac atggtgggct agcaagggcg aattcgcggc cgctaaattc aattcgccct 
     1381 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc 
     1441 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata 
     1501 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctatac gtacggcagt 
     1561 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg 
     1621 atattattga cacgccgggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt 
     1681 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca 
     1741 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc 
     1801 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa 
     1861 tgtcaggcat gagattatca aaaaggatct tcacctagat ccttttcacg tagaaagcca 
     1921 gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg 
     1981 aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag 
     2041 actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta 
     2101 aggttgggaa gccctgcaaa gtaaactgga tggctttctc gccgccaagg atctgatggc 
     2161 gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag 
     2221 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 
     2281 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 
     2341 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag 
     2401 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 
     2461 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 
     2521 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 
     2581 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 
     2641 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 
     2701 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg 
     2761 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 
     2821 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 
     2881 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 
     2941 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 
     3001 gaattattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta 
     3061 tttcacaccg catacaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 
     3121 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 
     3181 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 
     3241 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 
     3301 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 
     3361 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 
     3421 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 
     3481 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 
     3541 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 
     3601 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 
     3661 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 
     3721 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 
     3781 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 
     3841 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 
     3901 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 
     3961 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 
     4021 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 
     4081 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 
     4141 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 
     4201 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 
     4261 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 
     4321 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 
     4381 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 
     4441 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 
     4501 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 
     4561 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 
     4621 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 
     4681 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 
     4741 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 
     4801 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggg 
     4861 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 
     4921 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 
     4981 cgagtcagtg agcgaggaag cggaag 
//
