LOCUS       pCAhIFNGSTm             6738 bp    DNA     circular     29-OCT-2007
DEFINITION  LMBP 4482.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4482
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 29-OCT-2007
            
COMMENT     VNTNAME|pCAhIFNGSTm|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 149 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 2489 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 5 0 0 "")) 
COMMENT       (CObList 
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COMMENT        #5=(CFSignal (CObList) "mature hST6GalI mutant" 4 0 0 149 1211 0 
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COMMENT        #6=(CFSignal (CObList) "\337-globin polyA" 43 0 0 2028 2119 0 
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COMMENT        #7=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 2120 2476 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2120..2476") 
COMMENT        #8=(CFSignal (CObList) "lac promoter" 30 0 1 2554 2636 0 (CStringList) 
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COMMENT        #9=(CFSignal (CObList) "SV40 ori" 33 0 0 2778 2853 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pCAhIFNGSTm'" 1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
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FEATURES             Location/Qualifiers
     CDS             69..137
                     /vntifkey="4"
                     /label=hIFNG\signal\sequence
                     /note="human interferon gamma signal sequence"
     CDS             149..1211
                     /vntifkey="4"
                     /label=mature\hST6GalI\mutant
                     /note="mutated human alpha 2,6-sialyltransferase mature sequence"
     terminator      2028..2119
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     misc_feature    2120..2476
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     promoter        complement(2554..2636)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      2778..2853
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      2884..2958
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(2884..2958)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(3073..3474)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(4015..4875)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
     promoter        5011..5388
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     promoter        5394..6735
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     intron          5763..6687
                     /vntifkey="15"
                     /label=intron
                     /note="chicken beta-actin/rabbit beta-globin hybrid intron"
BASE COUNT     1584 a      1722 c      1741 g      1691 t 
ORIGIN
        1 ttcctcgagg tcagcttgat acaagaacta ctgatttcaa cttctttggc ttaattctct 
       61 cggaaacgat gaaatataca agttatatct tggcttttca gctctgcatc gttttgggtt 
      121 ctcttggctg ttactgccag gacccagcgg ccgccatggg gtctgattcc cagtctgtat 
      181 cctcaagcag cacccaggac ccccacaggg gccgccagac cctcggcagt ctcagaggcc 
      241 tagccaaggc caaaccagag gcctccttcc aggtgtggaa caaggacagc tcttccaaaa 
      301 accttatccc taggctgcaa aagatctgga agaattacct aagcatgaac aagtacaaag 
      361 tgtcctacaa ggggccagga ccaggcatca agttcagtgc agaggccctg cgctgccacc 
      421 tccgggacca tgtgaatgta tccatggtag aggtcacaga ttttcccttc aatacctctg 
      481 aatgggaggg ttatctgccc aaggagagca ttaggaccaa ggctgggcct tggggcaggt 
      541 gtgctgttgt gtcgtcagcg ggatctctga agtcctccca actaggcaga gaaatcgatg 
      601 atcatgacgc agtcctgagg tttaatgggg cacccacagc caacttccaa caagatgtgg 
      661 gcacaaaaac taccattcgc ctgatgaact ctcagttggt taccacagag aagcgcttcc 
      721 tcaaagacag tttgtacaat gaaggaatcc taattgtatg ggacccatct gtataccact 
      781 cagatatccc aaagtggtac cagaatccgg attataattt ctttaacaac tacaagactt 
      841 atcgtaagct gcaccccaat cagccctttt acatcctcaa gccccagatg ccttgggagc 
      901 tatgggacat tcttcaagaa atctccccag aagagattca gccaaacccc ccatcctctg 
      961 ggatgcttgg tatcatcatc atgatgacgc tgtgtgacca ggtggatatt tatgagttcc 
     1021 tcccatccaa gcgcaagact gacgtgtgct actactacca gaagttcttc gatagtgcct 
     1081 gcacgatggg tgcctaccac ccgctgctct atgagaagaa tttggtgaag catctcaacc 
     1141 agggcacaga tgaggacatc tacctgcttg gaaaagccac actgcctggc ttccggacca 
     1201 ttcactgcta agcacaggct cctcactctt ctccatcagg cattaaatga atggtctctt 
     1261 ggcctatgta aaagaagcag aaaaccttaa gaaatatttt aatgcaggtc attcagatgt 
     1321 agcggataat ggaactcttt tcttaggcat tttgaagaat tggaaagagg agagtgacag 
     1381 aaaaataatg cagagccaaa ttgtctcctt ttacttcaaa ctttttaaaa actttaaaga 
     1441 tgaccagagc atccaaaaga gtgtggagac catcaaggaa gacatgaatg tcaagttttt 
     1501 caatagcaac aaaaagaaac gagatgactt cgaaaagctg actaattatt cggtaactga 
     1561 cttgaatgtc caacgcaaag caatacatga actcatccaa gtgatggctg aactgtcgcc 
     1621 agcagctaaa acagggaagc gaaaaaggag tcagatgctg tttcgaggtc gaagagcatc 
     1681 ccagtaatgg ttgtcctgcc tgcaatattt gaattttaaa tctaaatcta tttattaata 
     1741 tttaacatta tttatatggg gaatatattt ttagactcat caatcaaata agtatttata 
     1801 atagcaactt ttgtgtaatg aaaatgaata tctattaata tatgtattat ttataattcc 
     1861 tatatcctgt gactgtctca cttaatcctt tgttttctga ctaattaggc aaggctatgt 
     1921 gattacaagg ctttatctca ggggccaact aggcagccaa cctaagcaag acctaggctt 
     1981 ttgcaaaaag ctcaagctcc aatgccctgg ctcacaaata ccactgagat ctttttccct 
     2041 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa 
     2101 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga 
     2161 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa 
     2221 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata 
     2281 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga 
     2341 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac 
     2401 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc 
     2461 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg 
     2521 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 
     2581 aagtgtaaag cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
     2641 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc 
     2701 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 
     2761 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 
     2821 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 
     2881 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 
     2941 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 
     3001 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 
     3061 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 
     3121 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga 
     3181 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 
     3241 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 
     3301 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 
     3361 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 
     3421 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 
     3481 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 
     3541 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 
     3601 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 
     3661 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 
     3721 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
     3781 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
     3841 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 
     3901 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 
     3961 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 
     4021 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
     4081 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 
     4141 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 
     4201 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 
     4261 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 
     4321 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 
     4381 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 
     4441 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 
     4501 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 
     4561 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 
     4621 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 
     4681 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 
     4741 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 
     4801 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 
     4861 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 
     4921 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 
     4981 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt 
     5041 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta 
     5101 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga 
     5161 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt 
     5221 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta 
     5281 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg 
     5341 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga 
     5401 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt 
     5461 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc 
     5521 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag 
     5581 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 
     5641 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc 
     5701 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac 
     5761 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac 
     5821 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt 
     5881 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg 
     5941 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc 
     6001 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg 
     6061 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg 
     6121 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg 
     6181 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt 
     6241 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg 
     6301 aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat 
     6361 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg 
     6421 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc 
     6481 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc 
     6541 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg 
     6601 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat 
     6661 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct 
     6721 catcattttg gcaaagaa 
//
