ID pCAhIFNGSTm standard; circular DNA; ; 6738 BP. DE LMBP 4482. CC Accession number: LMBP 4482 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 29-OCT-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 69..137 FT /vntifkey="4" FT /label=hIFNG\signal\sequence FT /note="human interferon gamma signal sequence" FT CDS 149..1211 FT /vntifkey="4" FT /label=mature\hST6GalI\mutant FT /note="mutated human alpha 2,6-sialyltransferase mature sequence" FT terminator 2028..2119 FT /vntifkey="43" FT /label=ß-globin\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT misc_feature 2120..2476 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of the rabbit ß-globin gene" FT promoter complement(2554..2636) FT /vntifkey="30" FT /label=lac\promoter FT /note="E. coli lac operon promoter" FT rep_origin 2778..2853 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT terminator 2884..2958 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(2884..2958) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(3073..3474) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(4015..4875) FT /vntifkey="4" FT /label=AMP FT /note="antibiotic resistance gene; coding sequence" FT promoter 5011..5388 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT /note="human CMV-IE enhancer" FT promoter 5394..6735 FT /vntifkey="29" FT /label=AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter" FT intron 5763..6687 FT /vntifkey="15" FT /label=intron FT /note="chicken beta-actin/rabbit beta-globin hybrid intron" SQ Sequence 6738 BP; 1584 A; 1722 C; 1741 G; 1691 t; ttcctcgagg tcagcttgat acaagaacta ctgatttcaa cttctttggc ttaattctct 60 cggaaacgat gaaatataca agttatatct tggcttttca gctctgcatc gttttgggtt 120 ctcttggctg ttactgccag gacccagcgg ccgccatggg gtctgattcc cagtctgtat 180 cctcaagcag cacccaggac ccccacaggg gccgccagac cctcggcagt ctcagaggcc 240 tagccaaggc caaaccagag gcctccttcc aggtgtggaa caaggacagc tcttccaaaa 300 accttatccc taggctgcaa aagatctgga agaattacct aagcatgaac aagtacaaag 360 tgtcctacaa ggggccagga ccaggcatca agttcagtgc agaggccctg cgctgccacc 420 tccgggacca tgtgaatgta tccatggtag aggtcacaga ttttcccttc aatacctctg 480 aatgggaggg ttatctgccc aaggagagca ttaggaccaa ggctgggcct tggggcaggt 540 gtgctgttgt gtcgtcagcg ggatctctga agtcctccca actaggcaga gaaatcgatg 600 atcatgacgc agtcctgagg tttaatgggg cacccacagc caacttccaa caagatgtgg 660 gcacaaaaac taccattcgc ctgatgaact ctcagttggt taccacagag aagcgcttcc 720 tcaaagacag tttgtacaat gaaggaatcc taattgtatg ggacccatct gtataccact 780 cagatatccc aaagtggtac cagaatccgg attataattt ctttaacaac tacaagactt 840 atcgtaagct gcaccccaat cagccctttt acatcctcaa gccccagatg ccttgggagc 900 tatgggacat tcttcaagaa atctccccag aagagattca gccaaacccc ccatcctctg 960 ggatgcttgg tatcatcatc atgatgacgc tgtgtgacca ggtggatatt tatgagttcc 1020 tcccatccaa gcgcaagact gacgtgtgct actactacca gaagttcttc gatagtgcct 1080 gcacgatggg tgcctaccac ccgctgctct atgagaagaa tttggtgaag catctcaacc 1140 agggcacaga tgaggacatc tacctgcttg gaaaagccac actgcctggc ttccggacca 1200 ttcactgcta agcacaggct cctcactctt ctccatcagg cattaaatga atggtctctt 1260 ggcctatgta aaagaagcag aaaaccttaa gaaatatttt aatgcaggtc attcagatgt 1320 agcggataat ggaactcttt tcttaggcat tttgaagaat tggaaagagg agagtgacag 1380 aaaaataatg cagagccaaa ttgtctcctt ttacttcaaa ctttttaaaa actttaaaga 1440 tgaccagagc atccaaaaga gtgtggagac catcaaggaa gacatgaatg tcaagttttt 1500 caatagcaac aaaaagaaac gagatgactt cgaaaagctg actaattatt cggtaactga 1560 cttgaatgtc caacgcaaag caatacatga actcatccaa gtgatggctg aactgtcgcc 1620 agcagctaaa acagggaagc gaaaaaggag tcagatgctg tttcgaggtc gaagagcatc 1680 ccagtaatgg ttgtcctgcc tgcaatattt gaattttaaa tctaaatcta tttattaata 1740 tttaacatta tttatatggg gaatatattt ttagactcat caatcaaata agtatttata 1800 atagcaactt ttgtgtaatg aaaatgaata tctattaata tatgtattat ttataattcc 1860 tatatcctgt gactgtctca cttaatcctt tgttttctga ctaattaggc aaggctatgt 1920 gattacaagg ctttatctca ggggccaact aggcagccaa cctaagcaag acctaggctt 1980 ttgcaaaaag ctcaagctcc aatgccctgg ctcacaaata ccactgagat ctttttccct 2040 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa 2100 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga 2160 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa 2220 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata 2280 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga 2340 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac 2400 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc 2460 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg 2520 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 2580 aagtgtaaag cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 2640 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc 2700 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 2760 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 2820 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 2880 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 2940 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 3000 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 3060 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 3120 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga 3180 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 3240 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 3300 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 3360 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 3420 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 3480 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 3540 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 3600 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 3660 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 3720 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 3780 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 3840 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 3900 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 3960 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 4020 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 4080 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 4140 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 4200 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 4260 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 4320 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 4380 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 4440 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 4500 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 4560 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 4620 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 4680 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 4740 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 4800 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 4860 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 4920 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 4980 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt 5040 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta 5100 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga 5160 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt 5220 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta 5280 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg 5340 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga 5400 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt 5460 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc 5520 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag 5580 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 5640 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc 5700 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac 5760 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac 5820 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt 5880 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg 5940 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc 6000 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg 6060 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg 6120 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg 6180 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt 6240 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg 6300 aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat 6360 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg 6420 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc 6480 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc 6540 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg 6600 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat 6660 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct 6720 catcattttg gcaaagaa 6738 //