LOCUS       pAJ401mds1              7720 bp    DNA     circular      6-SEP-2011
DEFINITION  LMBP 4485.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4485
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 6-SEP-2011
            
            
            
            
COMMENT     VNTNAME|pAJ401mds1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAJ401mds1" 0 7720 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "AflIII" "BglII" "ClaI" "NcoI" "SphI" "XbaI" "XhoI" "BamHI" 
COMMENT         "EcoRI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 
COMMENT        0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 7720 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "AflIII" "acrygt" 2 0 2 4726 0 5841 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BglII" "agatct" 2 0 5 1311 0 3400 0 3444 0 
COMMENT            3956 0 5134 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "ClaI" "atcgat" 3 0 3 1909 0 5261 0 5777 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 4700 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SphI" "gcatgc" 6 0 1 3007 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 443 0 5341 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 2280 0 3495 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 437 0 2016 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1328 0 0 "")) 
COMMENT       (CObList 
COMMENT        #9=(CFSignal (CObList) "lac promoter" 30 0 1 3831 3914 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3831..3914)") 
COMMENT        #10=(CFSignal (CObList) "URA3" 4 0 1 4106 4909 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4106..4909)") 
COMMENT        #11=(CFSignal (CObList) "ampicillin resistance" 4 0 1 6660 7520 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(6660..7520)") 
COMMENT        #12=(CFSignal (CObList) "PGK promoter" 29 0 0 486 1305 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "486..1305") 
COMMENT        #13=(CFSignal (CObList) "PGK terminator" 43 0 0 3506 3769 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3506..3769") 
COMMENT        #14=(CFSignal (CObList) "mds1" 4 0 0 1673 3244 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1673..3244") 
COMMENT        #15=(CFSignal (CObList) "2mu ori" 33 0 0 5343 5774 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5343..5774") 
COMMENT        #16=(CFSignal (CObList) "pMB1 ori" 33 0 1 5781 6119 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5781..6119)") 
COMMENT        #17=(CFSignal (CObList) "5' UTR mds1" 21 0 0 1341 1672 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1341..1672") 
COMMENT        #18=(CFSignal (CObList) "3' UTR mds1" 21 0 0 3245 3490 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3245..3490")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #19=(CFSignal (CObList) "LMBP 4485" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #20=(CFSignal (CObList) "Last modified: 6-SEP-2011" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                   "" 0 4) 
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COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 0 178) 
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COMMENT                          "DNA Plasmid 'pAJ401mds1'" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4485." 
COMMENT                          1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 09/06/11 10:36" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7720 bp" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 5-SEP-2011" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #35=(CRefLinePar 
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COMMENT                                 "" 1))) "LMBP 4485") 
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COMMENT                                   #44=(CLinePar 
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COMMENT                                        "Start: 1673  End: 3244" 1) 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "T. reesei 1,2-alpha-D-mannosidase coding sequence" 
COMMENT                                        1) 
COMMENT                                   #46=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #47=(CLinePar 
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COMMENT                                        "   1673..3244" 1))) "mds1") 
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COMMENT                                        "Start: 4106  End: 4909 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "S. cerevisiae URA3 coding sequence" 1) 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                        "Start: 6660  End: 7520 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene" 1) 
COMMENT                                   #56=(CLinePar 
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COMMENT                                   #57=(CLinePar 
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COMMENT                                        "   complement(6660..7520)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (3 total)") 
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COMMENT                                   #66=(CLinePar 
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COMMENT                                        "   3245..3490" 1))) "3' UTR mds1"))) 
COMMENT                          "Misc. Feature (2 total)") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 486   End: 1305" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "S. cerevisiae phosphoglycerate kinase promoter" 
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COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   486..1305" 1))) "PGK promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                                        "Start: 3831  End: 3914 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #76=(CLinePar 
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COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3831..3914)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                        "Start: 5343  End: 5774" 1) 
COMMENT                                   #81=(CLinePar 
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COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5343..5774" 1))) "2mu ori") 
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COMMENT                                        "Start: 5781  End: 6119 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "origin plasmid" 1) 
COMMENT                                   #86=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(5781..6119)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
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COMMENT                                        "Start: 3506  End: 3769" 1) 
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COMMENT                                   #92=(CLinePar 
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COMMENT                                        "   3506..3769" 1))) "PGK terminator"))) 
COMMENT                          "Terminator (1 total)"))) "Feature Map") 
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COMMENT                                        " N1: 4700 " 1))))) "NcoI: 1 site") 1 5 
COMMENT                          "CCATGG" "GGTACC") 
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COMMENT                          "GCATGC" "CGTACG") 
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COMMENT                                           " N1: 4726 " 1) 
COMMENT                                     #106=(CLinePar 
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COMMENT                                           " N2: 5841 " 1))))) "AflIII: 2 sites") 
COMMENT                           1 5 "ACRYGT" "TGYRCA") 
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COMMENT                                     #110=(CLinePar 
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COMMENT                                           " N2: 5341 " 1))))) "XbaI: 2 sites") 
COMMENT                           1 5 "TCTAGA" "AGATCT") 
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COMMENT                                           " N2: 3495 " 1))))) "XhoI: 2 sites") 
COMMENT                           1 5 "CTCGAG" "GAGCTC") 
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COMMENT                                           " N1: 437 " 1) 
COMMENT                                     #118=(CLinePar 
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COMMENT                                           " N2: 2016 " 1))))) "BamHI: 2 sites") 
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COMMENT                                           " N3: 5777 " 1))))) "ClaI: 3 sites") 
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COMMENT                                           " N3: 3444 " 1) 
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COMMENT                                           " N4: 3956 " 1) 
COMMENT                                     #130=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #1# 5) 1 2 2 0 191) 
COMMENT                                           " N5: 5134 " 1))))) "BglII: 5 sites") 
COMMENT                           1 5 "AGATCT" "TCTAGA"))) 
COMMENT                   "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     promoter        complement(3831..3914)
                     /vntifkey="30"
                     /label=lac\promoter
     CDS             complement(4106..4909)
                     /vntifkey="4"
                     /label=URA3
                     /note="S. cerevisiae URA3 coding sequence"
     CDS             complement(6660..7520)
                     /vntifkey="4"
                     /label=ampicillin\resistance
                     /note="antibiotic resistance gene"
     promoter        486..1305
                     /vntifkey="29"
                     /label=PGK\promoter
                     /note="S. cerevisiae phosphoglycerate kinase promoter"
     terminator      3506..3769
                     /vntifkey="43"
                     /label=PGK\terminator
     CDS             1673..3244
                     /vntifkey="4"
                     /label=mds1
                     /note="T. reesei 1,2-alpha-D-mannosidase coding sequence"
     rep_origin      5343..5774
                     /vntifkey="33"
                     /label=2mu\ori
     rep_origin      complement(5781..6119)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     misc_feature    1341..1672
                     /vntifkey="21"
                     /label=5'\UTR\mds1
     misc_feature    3245..3490
                     /vntifkey="21"
                     /label=3'\UTR\mds1
BASE COUNT     1983 a      1897 c      1732 g      2105 t         3 others
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ggccgatgcg gccgcaattc 
      421 gagctcggta cccggggatc ctctagagtc gataaaaaaa aaaaaaaaaa aaaaaaaaaa 
      481 aaaaaattaa tttttttttc tttcctcttt ttattaacct taatttttat tttagattcc 
      541 tgacttcaac tcaagacgca cagatattat aacatctgca taataggcat ttgcaagaat 
      601 tactcgtgag taaggaaaga gtgaggaact atcgcatacc tgcatttaaa gatgccgatt 
      661 tgggcgcgaa tcctttattt tggcttcacc ctcatactat tatcagggcc agaaaaagga 
      721 agtgtttccc tccttcttga attgatgtta ccctcataaa gcacgtggcc tcttatcgag 
      781 aaagaaatta ccgtcgctcg tgatttgttt gcaaaaagaa caaaactgaa aaaacccaga 
      841 cacgctcgac ttcctgtctt cctattgatt gcagcttcca atttcgtcac acaacaaggt 
      901 cctagcgacg gctcacaggt tttgtaacaa gcaatcgaag gttctggaat ggcgggaaag 
      961 ggtttagtac cacatgctat gatgcccact gtgatctcca gagcaaagtt cgttcgatcg 
     1021 tactgttact ctctctcttt caaacagaat tgtccgaatc gtgtgacaac aacagcctgt 
     1081 tctcacacac tcttttcttc taaccaaggg ggtggtttag tttagtagaa cctcgtgaaa 
     1141 cttacattta catatatata aacttgcata aattggtcaa tgcaagaaat agatatttgg 
     1201 tcttttctaa ttcgtagttt ttcaagttct tagatgcttt ctttttctct tttttacaga 
     1261 tcatcaagga agtaattatc tactttttac aacaaatata aaaaccaaaa gatctacgta 
     1321 ctcgaagaat tcggcacgag cgcatctgca gggggcgcca tgtcttaaat tggaagagga 
     1381 ggaattttat gcttcgcatg aggtacccgc ttggccgctt gaagaacaat tcgcattctg 
     1441 gtggcgtagg cagtgtcgcg tcgcatgagg gaccccctct tctcattctc ctggctctgc 
     1501 tgattccgga ggaggaggag gagcatttga gaaagcattg aaatggacgt ctctcccacc 
     1561 cgtggcactt ttatctgcag ccccgtctca tgccttgagc ctgggcaagt gtctctctcc 
     1621 tcttcgcctt ggcgtccttg ttgtcaaagc ctgaactaca gctgctgcca acatgagatt 
     1681 ccctagcagc tccgtccttg ccctcgggct catcggacct gcgctggcgt atccaaagcc 
     1741 gggcgccaca aaacgtggat ctcccaaccc tacgagggcg gcagcagtca aggccgcatt 
     1801 ccagacgtcg tggaacgctt accaccattt tgcctttccc catgacgacc tccacccggt 
     1861 cagcaacagc tttgatgatg agagaaacgg ctggggctcg tcggcaatcg atggcttgga 
     1921 cacggctatc ctcatggggg atgccgacat tgtgaacacg atccttcagt atgtaccgca 
     1981 gatcaacttc accacgactg cggttgccaa ccaaggatcc tccgtgttcg agaccaacat 
     2041 tcggtacctc ggtggcctgc tttctgccta tgacctgttg cgaggtcctt tcagctcctt 
     2101 ggcgacaaac cagaccctgg taaacagcct tctgaggcag gctcaaacac tggccaacgg 
     2161 cctcaaggtt gcgttcacca ctcccagcgg tgtcccggac cctaccgtct tcttcaaccc 
     2221 tactgtccgg agaagtggtg catctagcaa caacgtcgct gaaattggaa gcctggtgct 
     2281 cgagtggaca cggttgagcg acctgacggg aaacccgcag tatgcccagc ttgcgcagaa 
     2341 gggcgagtcg tatctcctga atccaaaggg aagcccggag gcatggcctg gcctgattgg 
     2401 aacgtttgtc agcacgagca acggtacctt tcaggatagc agcggcagct ggtccggcct 
     2461 catggacagc ttctacgagt acctgatcaa gatgtacctg tacgacccgg ttgcgtttgc 
     2521 acactacaag gatcgctggg tccttggtgc cgactcgacc attgggcatc tcggctctca 
     2581 cccgtcgacg cgcaaggact tgaccttttt gtcttcgtac aacggacagt ctacgtcgcc 
     2641 aaactcagga catttggcca gttttggcgg tggcaacttc atcttgggag gcattctcct 
     2701 gaacgagcaa aagtacattg actttggaat caagcttgcc agctcgtact ttggcacgta 
     2761 cacccagacg gcttctggaa tcggccccga aggcttcgcg tgggtggaca gcgtgacggg 
     2821 cgccggcggc tcgccgccct cgtcccagtc cgggttctac tcgtcggcag gattctgggt 
     2881 gacggcaccg tattacatcc tgcggccgga gacgctggag agcttgtact acgcataccg 
     2941 cgtcacgggc gactccaagt ggcaggacct ggcgtgggaa gcgttgagtg ccattgagga 
     3001 cgcatgccgc gccggcagcg cgtactcgtc catcaacgac gtgacgcagg ccaacggcgg 
     3061 gggtgcctct gacgatatgg agagcttctg gtttgccgag gcgctcaagt atgcgtacct 
     3121 gatctttgcg gaggagtcgg atgtgcaggt gcaggccacc ggcgggaaca aatttgtctt 
     3181 taacacggag gcgcacccct ttagcatccg ttcatcatca cgacggggcg gccaccttgc 
     3241 ttaaaggcgc tgagagttct ctcgcctgtt tttttgctcg tattattcat tttctgctgc 
     3301 tcgtcccgaa tcgctccgta gatattttga cacatgcgtc tggatagagc ggctgggacc 
     3361 accgaatatg gtatgtcatt cgagatatga agcgcaagag atctttaaga tccaagttct 
     3421 ctggtattat actggatgag atagatctat aggaaaccct gtccgaattg tgtggaaaaa 
     3481 aaaaaaaaaa nnnctcgagt aattccgata gatcaatttt tttcttttct ctttccccat 
     3541 cctttacgct aaaataatag tttattttat tttttgaata ttttttattt atatacgtat 
     3601 atatagacta ttatttactt ttaatagatt attaagattt ttattaaaaa aaaattcgtc 
     3661 cctcttttta atgcctttta tgcagttttt ttttcccatt cgatatttct atgttcgggt 
     3721 ttcagcgtat tttaagttta ataactcgaa aattctgcgt ttcgaaaaaa agcttggcgt 
     3781 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 
     3841 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 
     3901 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcagatct 
     3961 gagctttttc tttccaattt tttttttttc gtcattataa aaatcattac gaccgagatt 
     4021 cccggccggg taataactga tataattaaa ttgaagctct aatttgtgag tttagtatac 
     4081 atgcatttac ttataataca gttttttagt tttgctggcc gcatcttctc aaatatgctt 
     4141 cccagcctgc ttttctgtaa cgttcaccct ctaccttagc atcccttccc tttgcaaata 
     4201 gtcctcttcc aacaataata atgtcagatc ctgtagagac cacatcatcc acggttctat 
     4261 actgttgacc caatgcgtct cccttgtcat ctaaacccac accgggtgtc ataatcaacc 
     4321 aatcgtaacc ttcatctctt ccacccatgt ctctttgagc aataaagccg ataacaaaat 
     4381 ctttgtcgct cttcgcaatg tcaacagtac ccttagtata ttctccagta gatagggagc 
     4441 ccttgcatga caattctgct aacatcaaaa ggcctctagg ttcctttgtt acttcttctg 
     4501 ccgcctgctt caaaccgcta acaatacctg ggcccaccac accgtgtgca ttcgtaatgt 
     4561 ctgcccattc tgctattctg tatacacccg cagagtactg caatttgact gtattaccaa 
     4621 tgtcagcaaa ttttctgtct tcgaagagta aaaaattgta cttggcggat aatgccttta 
     4681 gcggcttaac tgtgccctcc atggaaaaat cagtcaagat atccacatgt gtttttagta 
     4741 aacaaatttt gggacctaat gcttcaacta actccagtaa ttccttggtg gtacgaacat 
     4801 ccaatgaagc acacaagttt gtttgctttt cgtgcatgat attaaatagc ttggcagcaa 
     4861 caggactagg atgagtagca gcacgttcct tatatgtagc tttcgacatg atttatcttc 
     4921 gtttccggtt tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc 
     4981 aacactacat atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt 
     5041 ctgttcggag attaccgaat caaaaaaatt tcaaagaaac cgaaatcaaa aaaaagaata 
     5101 aaaaaaaaat gatgaattga attgaaaagc tcagatctgc tgcattaatg aatcggccaa 
     5161 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 
     5221 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagcat cgatggcagc tcgcgttgca 
     5281 ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc tattccgaag ttcctattct 
     5341 ctagaaagta taggaacttc agagcgcttt tgaaaaccaa aagcgctctg aagacgcact 
     5401 ttcaaaaaac caaaaacgca ccggactgta acgagctact aaaatattgc gaataccgct 
     5461 tccacaaaca ttgctcaaaa gtatctcttt gctatatatc tctgtgctat atccctatat 
     5521 aacctaccca tccacctttc gctccttgaa cttgcatcta aactcgacct ctacattttt 
     5581 tatgtttatc tctagtatta ctctttagac aaaaaaattg tagtaagaac tattcataga 
     5641 gtgaatcgaa aacaatacga aaatgtaaac atttcctata cgtagtatat agagacaaaa 
     5701 tagaagaaac cgttcataat tttctgacca atgaagaatc atcaacgcta tcactttctg 
     5761 ttcacaaagt atgcatcgat gctcactcaa aggtcggtaa tacggttatc cacagaatca 
     5821 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 
     5881 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 
     5941 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 
     6001 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 
     6061 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 
     6121 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 
     6181 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 
     6241 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 
     6301 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 
     6361 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 
     6421 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 
     6481 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 
     6541 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 
     6601 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 
     6661 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 
     6721 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 
     6781 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 
     6841 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 
     6901 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 
     6961 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 
     7021 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 
     7081 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 
     7141 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 
     7201 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 
     7261 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 
     7321 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 
     7381 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 
     7441 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 
     7501 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 
     7561 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 
     7621 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca 
     7681 ttaacctata aaaataggcg tatcacgagg ccctttcgtc 
//
