ID pAJ401mds1 standard; circular DNA; ; 7720 BP. DE LMBP 4485. CC Accession number: LMBP 4485 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 8-SEP-2011 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter complement(3831..3914) FT /vntifkey="30" FT /label=lac\promoter FT CDS complement(4106..4909) FT /vntifkey="4" FT /label=URA3 FT /note="S. cerevisiae URA3 coding sequence" FT CDS complement(6660..7520) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene" FT promoter 486..1305 FT /vntifkey="29" FT /label=PGK\promoter FT /note="S. cerevisiae phosphoglycerate kinase promoter" FT terminator 3506..3769 FT /vntifkey="43" FT /label=PGK\terminator FT CDS 1673..3244 FT /vntifkey="4" FT /label=mds1 FT /note="T. reesei 1,2-alpha-D-mannosidase coding sequence" FT rep_origin 5343..5774 FT /vntifkey="33" FT /label=2mu\ori FT rep_origin complement(5781..6119) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT misc_feature 1341..1672 FT /vntifkey="21" FT /label=5'\UTR\mds1 FT misc_feature 3245..3490 FT /vntifkey="21" FT /label=3'\UTR\mds1 SQ Sequence 7720 BP; 1983 A; 1897 C; 1732 G; 2105 t; 3 other; tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ggccgatgcg gccgcaattc 420 gagctcggta cccggggatc ctctagagtc gataaaaaaa aaaaaaaaaa aaaaaaaaaa 480 aaaaaattaa tttttttttc tttcctcttt ttattaacct taatttttat tttagattcc 540 tgacttcaac tcaagacgca cagatattat aacatctgca taataggcat ttgcaagaat 600 tactcgtgag taaggaaaga gtgaggaact atcgcatacc tgcatttaaa gatgccgatt 660 tgggcgcgaa tcctttattt tggcttcacc ctcatactat tatcagggcc agaaaaagga 720 agtgtttccc tccttcttga attgatgtta ccctcataaa gcacgtggcc tcttatcgag 780 aaagaaatta ccgtcgctcg tgatttgttt gcaaaaagaa caaaactgaa aaaacccaga 840 cacgctcgac ttcctgtctt cctattgatt gcagcttcca atttcgtcac acaacaaggt 900 cctagcgacg gctcacaggt tttgtaacaa gcaatcgaag gttctggaat ggcgggaaag 960 ggtttagtac cacatgctat gatgcccact gtgatctcca gagcaaagtt cgttcgatcg 1020 tactgttact ctctctcttt caaacagaat tgtccgaatc gtgtgacaac aacagcctgt 1080 tctcacacac tcttttcttc taaccaaggg ggtggtttag tttagtagaa cctcgtgaaa 1140 cttacattta catatatata aacttgcata aattggtcaa tgcaagaaat agatatttgg 1200 tcttttctaa ttcgtagttt ttcaagttct tagatgcttt ctttttctct tttttacaga 1260 tcatcaagga agtaattatc tactttttac aacaaatata aaaaccaaaa gatctacgta 1320 ctcgaagaat tcggcacgag cgcatctgca gggggcgcca tgtcttaaat tggaagagga 1380 ggaattttat gcttcgcatg aggtacccgc ttggccgctt gaagaacaat tcgcattctg 1440 gtggcgtagg cagtgtcgcg tcgcatgagg gaccccctct tctcattctc ctggctctgc 1500 tgattccgga ggaggaggag gagcatttga gaaagcattg aaatggacgt ctctcccacc 1560 cgtggcactt ttatctgcag ccccgtctca tgccttgagc ctgggcaagt gtctctctcc 1620 tcttcgcctt ggcgtccttg ttgtcaaagc ctgaactaca gctgctgcca acatgagatt 1680 ccctagcagc tccgtccttg ccctcgggct catcggacct gcgctggcgt atccaaagcc 1740 gggcgccaca aaacgtggat ctcccaaccc tacgagggcg gcagcagtca aggccgcatt 1800 ccagacgtcg tggaacgctt accaccattt tgcctttccc catgacgacc tccacccggt 1860 cagcaacagc tttgatgatg agagaaacgg ctggggctcg tcggcaatcg atggcttgga 1920 cacggctatc ctcatggggg atgccgacat tgtgaacacg atccttcagt atgtaccgca 1980 gatcaacttc accacgactg cggttgccaa ccaaggatcc tccgtgttcg agaccaacat 2040 tcggtacctc ggtggcctgc tttctgccta tgacctgttg cgaggtcctt tcagctcctt 2100 ggcgacaaac cagaccctgg taaacagcct tctgaggcag gctcaaacac tggccaacgg 2160 cctcaaggtt gcgttcacca ctcccagcgg tgtcccggac cctaccgtct tcttcaaccc 2220 tactgtccgg agaagtggtg catctagcaa caacgtcgct gaaattggaa gcctggtgct 2280 cgagtggaca cggttgagcg acctgacggg aaacccgcag tatgcccagc ttgcgcagaa 2340 gggcgagtcg tatctcctga atccaaaggg aagcccggag gcatggcctg gcctgattgg 2400 aacgtttgtc agcacgagca acggtacctt tcaggatagc agcggcagct ggtccggcct 2460 catggacagc ttctacgagt acctgatcaa gatgtacctg tacgacccgg ttgcgtttgc 2520 acactacaag gatcgctggg tccttggtgc cgactcgacc attgggcatc tcggctctca 2580 cccgtcgacg cgcaaggact tgaccttttt gtcttcgtac aacggacagt ctacgtcgcc 2640 aaactcagga catttggcca gttttggcgg tggcaacttc atcttgggag gcattctcct 2700 gaacgagcaa aagtacattg actttggaat caagcttgcc agctcgtact ttggcacgta 2760 cacccagacg gcttctggaa tcggccccga aggcttcgcg tgggtggaca gcgtgacggg 2820 cgccggcggc tcgccgccct cgtcccagtc cgggttctac tcgtcggcag gattctgggt 2880 gacggcaccg tattacatcc tgcggccgga gacgctggag agcttgtact acgcataccg 2940 cgtcacgggc gactccaagt ggcaggacct ggcgtgggaa gcgttgagtg ccattgagga 3000 cgcatgccgc gccggcagcg cgtactcgtc catcaacgac gtgacgcagg ccaacggcgg 3060 gggtgcctct gacgatatgg agagcttctg gtttgccgag gcgctcaagt atgcgtacct 3120 gatctttgcg gaggagtcgg atgtgcaggt gcaggccacc ggcgggaaca aatttgtctt 3180 taacacggag gcgcacccct ttagcatccg ttcatcatca cgacggggcg gccaccttgc 3240 ttaaaggcgc tgagagttct ctcgcctgtt tttttgctcg tattattcat tttctgctgc 3300 tcgtcccgaa tcgctccgta gatattttga cacatgcgtc tggatagagc ggctgggacc 3360 accgaatatg gtatgtcatt cgagatatga agcgcaagag atctttaaga tccaagttct 3420 ctggtattat actggatgag atagatctat aggaaaccct gtccgaattg tgtggaaaaa 3480 aaaaaaaaaa nnnctcgagt aattccgata gatcaatttt tttcttttct ctttccccat 3540 cctttacgct aaaataatag tttattttat tttttgaata ttttttattt atatacgtat 3600 atatagacta ttatttactt ttaatagatt attaagattt ttattaaaaa aaaattcgtc 3660 cctcttttta atgcctttta tgcagttttt ttttcccatt cgatatttct atgttcgggt 3720 ttcagcgtat tttaagttta ataactcgaa aattctgcgt ttcgaaaaaa agcttggcgt 3780 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 3840 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 3900 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcagatct 3960 gagctttttc tttccaattt tttttttttc gtcattataa aaatcattac gaccgagatt 4020 cccggccggg taataactga tataattaaa ttgaagctct aatttgtgag tttagtatac 4080 atgcatttac ttataataca gttttttagt tttgctggcc gcatcttctc aaatatgctt 4140 cccagcctgc ttttctgtaa cgttcaccct ctaccttagc atcccttccc tttgcaaata 4200 gtcctcttcc aacaataata atgtcagatc ctgtagagac cacatcatcc acggttctat 4260 actgttgacc caatgcgtct cccttgtcat ctaaacccac accgggtgtc ataatcaacc 4320 aatcgtaacc ttcatctctt ccacccatgt ctctttgagc aataaagccg ataacaaaat 4380 ctttgtcgct cttcgcaatg tcaacagtac ccttagtata ttctccagta gatagggagc 4440 ccttgcatga caattctgct aacatcaaaa ggcctctagg ttcctttgtt acttcttctg 4500 ccgcctgctt caaaccgcta acaatacctg ggcccaccac accgtgtgca ttcgtaatgt 4560 ctgcccattc tgctattctg tatacacccg cagagtactg caatttgact gtattaccaa 4620 tgtcagcaaa ttttctgtct tcgaagagta aaaaattgta cttggcggat aatgccttta 4680 gcggcttaac tgtgccctcc atggaaaaat cagtcaagat atccacatgt gtttttagta 4740 aacaaatttt gggacctaat gcttcaacta actccagtaa ttccttggtg gtacgaacat 4800 ccaatgaagc acacaagttt gtttgctttt cgtgcatgat attaaatagc ttggcagcaa 4860 caggactagg atgagtagca gcacgttcct tatatgtagc tttcgacatg atttatcttc 4920 gtttccggtt tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc 4980 aacactacat atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt 5040 ctgttcggag attaccgaat caaaaaaatt tcaaagaaac cgaaatcaaa aaaaagaata 5100 aaaaaaaaat gatgaattga attgaaaagc tcagatctgc tgcattaatg aatcggccaa 5160 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 5220 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagcat cgatggcagc tcgcgttgca 5280 ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc tattccgaag ttcctattct 5340 ctagaaagta taggaacttc agagcgcttt tgaaaaccaa aagcgctctg aagacgcact 5400 ttcaaaaaac caaaaacgca ccggactgta acgagctact aaaatattgc gaataccgct 5460 tccacaaaca ttgctcaaaa gtatctcttt gctatatatc tctgtgctat atccctatat 5520 aacctaccca tccacctttc gctccttgaa cttgcatcta aactcgacct ctacattttt 5580 tatgtttatc tctagtatta ctctttagac aaaaaaattg tagtaagaac tattcataga 5640 gtgaatcgaa aacaatacga aaatgtaaac atttcctata cgtagtatat agagacaaaa 5700 tagaagaaac cgttcataat tttctgacca atgaagaatc atcaacgcta tcactttctg 5760 ttcacaaagt atgcatcgat gctcactcaa aggtcggtaa tacggttatc cacagaatca 5820 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 5880 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 5940 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 6000 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 6060 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 6120 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 6180 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 6240 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 6300 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 6360 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 6420 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 6480 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 6540 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 6600 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 6660 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 6720 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 6780 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 6840 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 6900 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 6960 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 7020 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 7080 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 7140 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 7200 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 7260 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 7320 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 7380 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 7440 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 7500 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 7560 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 7620 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca 7680 ttaacctata aaaataggcg tatcacgagg ccctttcgtc 7720 //