LOCUS       pBlueBachsGCA1          7123 bp    DNA     circular      5-AUG-2010
DEFINITION  LMBP 4487.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4487
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 5-AUG-2010
            
            
COMMENT     VNTNAME|pBlueBachsGCA1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBlueBachsGCA1" 0 7123 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 926157325 0 "" 0 "" "" "" "" "" "" 
COMMENT             "pBlueBachsGCA1His" "" 0 0 1 187 1 187 1 187 0 "" "" "" "" "" "" 
COMMENT             (CObList) (CTerm 4 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 187 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 0 0 "" 0 "" "" "" "" "" "" "hsGCA1gene" "" 0 0 1 
COMMENT             2080 188 2267 1 2080 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 4 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 4 1 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #2=(CFragment 3 1 0 168638260 0 "" 0 "" "" "" "" "" "" 
COMMENT             "pBlueBachsGCA1His" "" 0 0 1 4856 2268 7123 2261 7116 0 "" "" "" "" 
COMMENT             "" "" (CObList) (CTerm 2 0 2261 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 4 1 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 1100 0 
COMMENT        1 0 (CHomObj 1 0 0 3 75) 
COMMENT        (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) (CWordArray) 
COMMENT        (CStringList "AvaI" "HindIII" "NcoI" "XbaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #3=(COligo "lacZ (1)" 
COMMENT             "ATGACCATGATTACGGATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGGTGCCGGAAAGCTGGCTGGAGTGCGATCTTCCTGAGGCCGATACTGTCGTCGTCCCCTCAAACTGGCAGATGCACGGTTACGATGCGCCCATCTACACCAACGTAACCTATCCCATTACGGTCAATCCGCCGTTTGTTCCCACGGAGAATCCGACGGGTTGTTACTCGCTCACATTTAATGTTGATGAAAGCTGGCTACAGGAAGGCCAGACGCGAATTATTTTTGATGGCGTTAACTCGGCGTTTCATCTGTGGTGCAACGGGCGCTGGGTCGGTTACGGCCAGGACAGTCGTTTGCCGTCTGAATTTGACCTGAGCGCATTTTTACGCGCCGGAGAAAACCGCCTCGCGGTGATGGTGCTGCGTTGGAGTGACGGCAGTTATCTGGAAGATCAGGATATGTGGCGGATGAGCGGCATTTTCCGTGACGTCTCGTTGCTGCATAAACCGACTACACAAATCAGCGATTTCCATGTTGCCACTCGCTTTAATGATGATTTCAGCCGCGCTGTACTGGAGGCTGAAGTTCAGATGTGCGGCGAGTTGCGTGACTACCTACGGGTAACAGTTTCTTTATGGCAGGGTGAAACGCAGGTCGCCAGCGGCACCGCGCCTTTCGGCGGTGAAATTATCGATGAGCGTGGTGGTTATGCCGATCGCGTCACACTACGTCTGAACGTCGAAAACCCGAAACTGTGGAGCGCCGAAATCCCGAATCTCTATCGTGCGGTGGTTGAACTGCACACCGCCGACGGCACGCTGATTGAAGCAGAAGCCTGCGATGTCGGTTTCC" 
COMMENT             "Fragment of ECOLAC; Length: 1000" 0 (CStringList) 0) 
COMMENT         #4=(COligo "lacZ (2)" 
COMMENT             "GCGAGGTGCGGATTGAAAATGGTCTGCTGCTGCTGAACGGCAAGCCGTTGCTGATTCGAGGCGTTAACCGTCACGAGCATCATCCTCTGCATGGTCAGGTCATGGATGAGCAGACGATGGTGCAGGATATCCTGCTGATGAAGCAGAACAACTTTAACGCCGTGCGCTGTTCGCATTATCCGAACCATCCGCTGTGGTACACGCTGTGCGACCGCTACGGCCTGTATGTGGTGGATGAAGCCAATATTGAAACCCACGGCATGGTGCCAATGAATCGTCTGACCGATGATCCGCGCTGGCTACCGGCGATGAGCGAACGCGTAACGCGAATGGTGCAGCGCGATCGTAATCACCCGAGTGTGATCATCTGGTCGCTGGGGAATGAATCAGGCCACGGCGCTAATCACGACGCGCTGTATCGCTGGATCAAATCTGTCGATCCTTCCCGCCCGGTGCAGTATGAAGGCGGCGGAGCCGACACCACGGCCACCGATATTATTTGCCCGATGTACGCGCGCGTGGATGAAGACCAGCCCTTCCCGGCTGTGCCGAAATGGTCCATCAAAAAATGGCTTTCGCTACCTGGAGAGACGCGCCCGCTGATCCTTTGCGAATACGCCCACGCGATGGGTAACAGTCTTGGCGGTTTCGCTAAATACTGGCAGGCGTTTCGTCAGTATCCCCGTTTACAGGGCGGCTTCGTCTGGGACTGGGTGGATCAGTCGCTGATTAAATATGATGAAAACGGCAACCCGTGGTCGGCTTACGGCGGTGATTTTGGCGATACGCCGAACGATCGCCAGTTCTGTATGAACGGTCTGGTCTTTGCCGACCGCACGCCGCATCCAGCGCTGACGGAAGCAAAACACCAGCAGCAGTTTTTCCAGTTCCGTTTATCCGGGCAAACCATCGAAGTGACCAGCGAATACCTGTTCCGTCATAGCGATAACGAGCTCCTGCACTGGATGGTGGCGCTGGATGGTAAGCCGCTGGCAAGCGG" 
COMMENT             "Fragment of ECOLAC; Length: 1000" 0 (CStringList) 0) 
COMMENT         #5=(COligo "lacZ (3)" 
COMMENT             "TGAAGTGCCTCTGGATGTCGCTCCACAAGGTAAACAGTTGATTGAACTGCCTGAACTACCGCAGCCGGAGAGCGCCGGGCAACTCTGGCTCACAGTACGCGTAGTGCAACCGAACGCGACCGCATGGTCAGAAGCCGGGCACATCAGCGCCTGGCAGCAGTGGCGTCTGGCGGAAAACCTCAGTGTGACGCTCCCCGCCGCGTCCCACGCCATCCCGCATCTGACCACCAGCGAAATGGATTTTTGCATCGAGCTGGGTAATAAGCGTTGGCAATTTAACCGCCAGTCAGGCTTTCTTTCACAGATGTGGATTGGCGATAAAAAACAACTGCTGACGCCGCTGCGCGATCAGTTCACCCGTGCACCGCTGGATAACGACATTGGCGTAAGTGAAGCGACCCGCATTGACCCTAACGCCTGGGTCGAACGCTGGAAGGCGGCGGGCCATTACCAGGCCGAAGCAGCGTTGTTGCAGTGCACGGCAGATACACTTGCTGATGCGGTGCTGATTACGACCGCTCACGCGTGGCAGCATCAGGGGAAAACCTTATTTATCAGCCGGAAAACCTACCGGATTGATGGTAGTGGTCAAATGGCGATTACCGTTGATGTTGAAGTGGCGAGCGATACACCGCATCCGGCGCGGATTGGCCTGAACTGCCAGCTGGCGCAGGTAGCAGAGCGGGTAAACTGGCTCGGATTAGGGCCGCAAGAAAACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAACGGTCTGCGCTGCGGGACGCGCGAATTGAATTATGGCCCACACCAGTGGCGCGGCGACTTCCAGTTCAACATCAGCCGCTACAGTCAACAGCAACTGATGGAAACCAGCCATCGCCATCTGCTGCACGCGGAAGAAGGCACATGGCTGAATATCGACGGTTTCCATATGGGGATTGGTGGCGACGACTCCTGG" 
COMMENT             "Fragment of ECOLAC; Length: 1000" 0 (CStringList) 0) 
COMMENT         #6=(COligo "lacZ (4)" 
COMMENT             "AGCCCGTCAGTATCGGCGGAATTCCAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTGTCAAAAATAA" 
COMMENT             "Fragment of ECOLAC; Length: 75" 0 (CStringList) 0) 
COMMENT         #7=(COligo "pMB1 ori" 
COMMENT             "CTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGC" 
COMMENT             "Fragment of pRH007; Length: 403" 0 (CStringList "ORI") 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 3307 3302 1 7123 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #8=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 3 148 0 168 0 1447 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2281 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 1889 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 174 0 0 "")) 
COMMENT       (CObList 
COMMENT        #12=(CFSignal (CObList) "ampicillin resistance" 4 0 0 3913 4773 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3913..4773") 
COMMENT        #13=(CFSignal (CObList) "5' lacZ fragment" 4 0 1 5709 6821 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(5709..6821)") 
COMMENT        #14=(CFSignal (CObList) "pMB1 ori" 33 0 0 5314 5708 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5314..5708") 
COMMENT        #15=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2385 2459 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2385..2459") 
COMMENT        #16=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2385 2459 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2385..2459)") 
COMMENT        #17=(CFSignal (CObList) "V5 epitope" 22 0 0 2292 2333 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "His-tag" 22 0 0 2343 2360 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2343..2360") 
COMMENT        #19=(CFSignal (CObList) "AcNPV-PH promoter" 29 0 0 7 95 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7..95") 
COMMENT        #20=(CFSignal (CObList) "AcNPV-ETL promoter" 29 0 1 6822 7096 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(6822..7096)") 
COMMENT        #21=(CFSignal (CObList) "hsGCA1" 4 0 0 191 2263 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "191..2263") 
COMMENT        #22=(CFSignal (CObList) "3' AcNPV ORF 1629 fragment" 4 0 1 2647 3525 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2647..3525)")) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #23=(CFSignal (CObList) "LMBP 4487" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #24=(CFSignal (CObList) "Last modified: 5-AUG-2010" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #25=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #26=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pBlueBachsGCA1" 2) 
COMMENT                   5 "" 0 4) 
COMMENT              #27=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pBlueBachsGCA1'" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4487." 
COMMENT                          1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 08/05/10 04:53" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7123 bp" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Constructed" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #36=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 29-JUL-2010" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #39=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #40=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #41=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #42=(CLinePar (CParagraph 0 (15 #23# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 4487") 
COMMENT                     #43=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #44=(CLinePar (CParagraph 0 (15 #24# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 5-AUG-2010"))) 
COMMENT                   "Annotations") 
COMMENT              #45=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 10 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #46=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (5 #0# 0) 1 2 1 0 195) 
COMMENT                           (CObjectList 
COMMENT                            #47=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "parent position: from 1 to 187" 1) 
COMMENT                            #48=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 187" 1) 
COMMENT                            #49=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 1 to 187" 1) 
COMMENT                            #50=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  Start of Molecule" 1))) 
COMMENT                                 "Left Terminus") 
COMMENT                            #52=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  Position 187" 1))) "Right Terminus"))) 
COMMENT                          "#1: FRAGMENT of <a href=\"vnti:dnarna/pBlueBachsGCA1His?sel=1-187\">pBlueBachsGCA1His</a> ") 
COMMENT                     #54=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (5 #1# 0) 1 2 1 0 195) 
COMMENT                           (CObjectList 
COMMENT                            #55=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "parent position: from 1 to 2080" 1) 
COMMENT                            #56=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 2080" 1) 
COMMENT                            #57=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 188 to 2267" 1) 
COMMENT                            #58=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  Start of Molecule" 1))) 
COMMENT                                 "Left Terminus") 
COMMENT                            #60=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  End of Molecule" 1))) 
COMMENT                                 "Right Terminus"))) 
COMMENT                          "#2: FRAGMENT of <a href=\"vnti:dnarna/hsGCA1gene?sel=1-2080\">hsGCA1gene</a> ") 
COMMENT                     #62=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (5 #2# 0) 1 2 1 0 195) 
COMMENT                           (CObjectList 
COMMENT                            #63=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "parent position: from 2261 to 7116" 1) 
COMMENT                            #64=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 4856" 1) 
COMMENT                            #65=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 2268 to 7123" 1) 
COMMENT                            #66=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  Position 2261" 1))) "Left Terminus") 
COMMENT                            #68=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  End of Molecule" 1))) 
COMMENT                                 "Right Terminus"))) 
COMMENT                          "#3: FRAGMENT of <a href=\"vnti:dnarna/pBlueBachsGCA1His?sel=2261-7116\">pBlueBachsGCA1His</a> "))) 
COMMENT                   "Component Fragments") 
COMMENT              #70=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #71=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 2 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #72=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 191 (3 #21# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 191   End: 2263" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   191..2263" 1))) "hsGCA1") 
COMMENT                            #76=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2647 (3 #22# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2647  End: 3525 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2647..3525)" 1))) 
COMMENT                                 "3' AcNPV ORF 1629 fragment") 
COMMENT                            #80=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3913 (3 #12# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3913  End: 4773" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3913..4773" 1))) 
COMMENT                                 "ampicillin resistance") 
COMMENT                            #84=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5709 (3 #13# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5709  End: 6821 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(5709..6821)" 1))) 
COMMENT                                 "5' lacZ fragment"))) "CDS (4 total)") 
COMMENT                     #88=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #89=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2292 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2292  End: 2333" 1))) 
COMMENT                                 "V5 epitope") 
COMMENT                            #91=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2343 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2343  End: 2360" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2343..2360" 1))) "His-tag"))) 
COMMENT                          "Misc. Marker (2 total)") 
COMMENT                     #95=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #96=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7     End: 95  " 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   7..95" 1))) "AcNPV-PH promoter") 
COMMENT                            #100=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 6822 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #101=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 6822  End: 7096 (Complementary)" 
COMMENT                                          1) 
COMMENT                                    #102=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #103=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(6822..7096)" 1))) 
COMMENT                                  "AcNPV-ETL promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #104=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #105=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5314 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #106=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5314  End: 5708" 1) 
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FEATURES             Location/Qualifiers
     promoter        7..95
                     /vntifkey="29"
                     /label=AcNPV-PH\promoter
     CDS             191..2263
                     /vntifkey="4"
                     /label=hsGCA1
     CDS             3913..4773
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             complement(5709..6821)
                     /vntifkey="4"
                     /label=5'\lacZ\fragment
     misc_marker     2343..2360
                     /vntifkey="22"
                     /label=His-tag
     CDS             complement(2647..3525)
                     /vntifkey="4"
                     /label=3'\AcNPV\ORF\1629\fragment
     rep_origin      5314..5708
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(6822..7096)
                     /vntifkey="29"
                     /label=AcNPV-ETL\promoter
     terminator      2385..2459
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(2385..2459)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     misc_marker     2292..2333
                     /vntifkey="22"
                     /label=V5\epitope
BASE COUNT     1864 a      1689 c      1712 g      1858 t 
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt 
       61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca 
      121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat 
      181 tgcccttacc atgttctgca cgaagctcaa ggatctcaag atcacaggag agtgtccttt 
      241 ctccttactg gcaccaggtc aagttcctaa cgagtcttca gaggaggcag caggaagctc 
      301 agagagctgc aaagcaaccg tgcccatctg tcaagacatt cctgagaaga acatacaaga 
      361 aagtcttcct caaagaaaaa ccagtcggag ccgagtctat cttcacactt tggcagagag 
      421 tatttgcaaa ctgattttcc cagagtttga acggctgaat gttgcacttc agagaacatt 
      481 ggcaaagcac aaaataaaag aaagcaggaa atctttggaa agagaagact ttgaaaaaac 
      541 aattgcagag caagcagttg cagcaggagt tccagtggag gttatcaaag aatctcttgg 
      601 tgaagaggtt tttaaaatat gttacgagga agatgaaaac atccttgggg tggttggagg 
      661 cacccttaaa gattttttaa acagcttcag tacccttctg aaacagagca gccattgcca 
      721 agaagcagga aaaaggggca ggcttgagga cgcctccatt ctatgcctgg ataaggagga 
      781 tgattttcta catgtttact acttcttccc taagagaacc acctccctga ttcttcccgg 
      841 catcataaag gcagctgctc acgtattata tgaaacggaa gtggaagtgt cgttaatgcc 
      901 tccctgcttc cataatgatt gcagcgagtt tgtgaatcag ccctacttgt tgtactccgt 
      961 tcacatgaaa agcaccaagc catccctgtc ccccagcaaa ccccagtcct cgctggtgat 
     1021 tcccacatcg ctattctgca agacatttcc attccatttc atgtttgaca aagatatgac 
     1081 aattctgcaa tttggcaatg gcatcagaag gctgatgaac aggagagact ttcaaggaaa 
     1141 gcctaatttt gaagaatact ttgaaattct gactccaaaa atcaaccaga cgtttagcgg 
     1201 gatcatgact atgttgaata tgcagtttgt tgtacgagtg aggagatggg acaactctgt 
     1261 gaagaaatct tcaagggtta tggacctcaa aggccaaatg atctacattg ttgaatccag 
     1321 tgcaatcttg tttttggggt caccctgtgt ggacagatta gaagatttta caggacgagg 
     1381 gctctacctc tcagacatcc caattcacaa tgcactgagg gatgtggtct taatagggga 
     1441 acaagcccga gctcaagatg gcctgaagaa gaggctgggg aagctgaagg ctacccttga 
     1501 gcaagcccac caagccctgg aggaggagaa gaaaaagaca gtagaccttc tgtgctccat 
     1561 atttccctgt gaggttgctc agcagctgtg gcaagggcaa gttgtgcaag ccaagaagtt 
     1621 cagtaatgtc accatgctct tctcagacat cgttgggttc actgccatct gctcccagtg 
     1681 ctcaccgctg caggtcatca ccatgctcaa tgcactgtac actcgcttcg accagcagtg 
     1741 tggagagctg gatgtctaca aggtggagac cattggcgat gcctattgtg tagctggggg 
     1801 attacacaaa gagagtgata ctcatgctgt tcagatagcg ctgatggccc tgaagatgat 
     1861 ggagctctct gatgaagtta tgtctcccca tggagaacct atcaagatgc gaattggact 
     1921 gcactctgga tcagtttttg ctggcgtcgt tggagttaaa atgccccgtt actgtctttt 
     1981 tggaaacaat gtcactctgg ctaacaaatt tgagtcctgc agtgtaccac gaaaaatcaa 
     2041 tgtcagccca acaacttaca gattactcaa agactgtcct ggtttcgtgt ttacccctcg 
     2101 atcaagggag gaacttccac caaacttccc tagtgaaatc cccggaatct gccattttct 
     2161 ggatgcttac caacaaggaa caaactcaaa accatgcttc caaaagaaag atgtggaaga 
     2221 tggcaatgcc aattttttag gcaaagcatc aggaatagat tagcaacaag ggcaattcga 
     2281 agcttaggcc tggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg 
     2341 gtcatcatca ccatcaccat tgacgtctct agcttggagt cgacaacttg tttattgcag 
     2401 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 
     2461 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat ctctcgactc 
     2521 tgctgaagag gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa aggagtttgc 
     2581 accagacgca cctctgttca ctggtccggc gtattaaaac acgatacatt gttattagta 
     2641 catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt 
     2701 taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt 
     2761 ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt 
     2821 tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt 
     2881 ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt 
     2941 cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt 
     3001 atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaataaagc 
     3061 ctggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca 
     3121 accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt 
     3181 aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattatttc 
     3241 tgattgcggg cgtttttggg cgggtttcaa tctaactgtg cccgatttta attcagacaa 
     3301 cacgttagaa agcgatggtg caggcggtgg taacatttca gacggcaaat ctactaatgg 
     3361 cggcggtggt ggagctgatg ataaatctac catcggtgga ggcgcaggcg gggctggcgg 
     3421 cggaggcgga ggcggaggtg gtggcggtga tgcagacggc ggtttaggct caaatgtctc 
     3481 tttaggcaac acagtcggca cctcaactat tgtactggtt tcgggcgatg gtgcactctc 
     3541 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct 
     3601 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc 
     3661 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag 
     3721 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg 
     3781 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 
     3841 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 
     3901 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 
     3961 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 
     4021 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 
     4081 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 
     4141 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 
     4201 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 
     4261 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 
     4321 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 
     4381 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 
     4441 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 
     4501 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 
     4561 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 
     4621 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 
     4681 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 
     4741 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 
     4801 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 
     4861 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 
     4921 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 
     4981 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 
     5041 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 
     5101 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 
     5161 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 
     5221 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 
     5281 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 
     5341 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 
     5401 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 
     5461 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 
     5521 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 
     5581 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 
     5641 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 
     5701 gaggaagcat cctgcaccat cgtctgctca tccatgacct gaccatgcag aggatgatgc 
     5761 tcgtgacggt taacgcctcg aatcagcaac ggcttgccgt tcagcagcag cagaccattt 
     5821 tcaatccgca cctcgcggaa accgacatcg caggcttctg cttcaatcag cgtgccgtcg 
     5881 gcggtgtgca gttcaaccac cgcacgatag agattcggga tttcggcgct ccacagtttc 
     5941 gggttttcga cgttcagacg tagtgtgacg cgatcggcat aaccaccacg ctcatcgata 
     6001 atttcaccgc cgaaaggcgc ggtgccgctg gcgacctgcg tttcaccctg ccataaagaa 
     6061 actgttaccc gtaggtagtc acgcaactcg ccgcacatct gaacttcagc ctccagtaca 
     6121 gcgcggctga aatcatcatt aaagcgagtg gcaacatgga aatcgctgat ttgtgtagtc 
     6181 ggtttatgca gcaacgagac gtcacggaaa atgccgctca tccgccacat atcctgatct 
     6241 tccagataac tgccgtcact ccagcgcagc accatcaccg cgaggcggtt ttctccggcg 
     6301 cgtaaaaatg cgctcaggtc aaattcagac ggcaaacgac tgtcctggcc gtaaccgacc 
     6361 cagcgcccgt tgcaccacag atgaaacgcc gagttaacgc catcaaaaat aattcgcgtc 
     6421 tggccttcct gtagccagct ttcatcaaca ttaaatgtga gcgagtaaca acccgtcgga 
     6481 ttctccgtgg gaacaaacgg cggattgacc gtaatgggat aggtcacgtt ggtgtagatg 
     6541 ggcgcatcgt aaccgtgcat ctgccagttt gaggggacga cgacagtatc ggcctcagga 
     6601 agatcgcact ccagccagct ttccggcacc gcttctggtg ccggaaacca ggcaaagcgc 
     6661 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta 
     6721 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg 
     6781 ttttcccagt cacgacgttg taaaacgacg ggatctatca tttttagcag tgattctaat 
     6841 tgcagctgct ctttgataca actaatttta cgacgacgat gcgagctttt attcaaccga 
     6901 gcgtgcatgt ttgcaatcgt gcaagcgtta tcaatttttc attatcgtat tgttgcacat 
     6961 caacaggctg gacaccacgt tgaactcgcc gcagttttgc ggcaagttgg acccgccgcg 
     7021 catccaatgc aaactttccg acattctgtt gcctacgaac gattgattct ttgtccattg 
     7081 atcgaagcga gtgccttcga ctttttcgtg tccagtgtgg ctt 
//
