ID pBlueBachsGCA1 standard; circular DNA; ; 7123 BP. DE LMBP 4487. CC Accession number: LMBP 4487 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 5-AUG-2010 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 7..95 FT /vntifkey="29" FT /label=AcNPV-PH\promoter FT CDS 191..2263 FT /vntifkey="4" FT /label=hsGCA1 FT CDS 3913..4773 FT /vntifkey="4" FT /label=ampicillin\resistance FT CDS complement(5709..6821) FT /vntifkey="4" FT /label=5'\lacZ\fragment FT misc_marker 2343..2360 FT /vntifkey="22" FT /label=His-tag FT CDS complement(2647..3525) FT /vntifkey="4" FT /label=3'\AcNPV\ORF\1629\fragment FT rep_origin 5314..5708 FT /vntifkey="33" FT /label=pMB1\ori FT promoter complement(6822..7096) FT /vntifkey="29" FT /label=AcNPV-ETL\promoter FT terminator 2385..2459 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(2385..2459) FT /vntifkey="43" FT /label=SV40\polyA\late FT misc_marker 2292..2333 FT /vntifkey="22" FT /label=V5\epitope SQ Sequence 7123 BP; 1864 A; 1689 C; 1712 G; 1858 t; gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt 60 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca 120 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat 180 tgcccttacc atgttctgca cgaagctcaa ggatctcaag atcacaggag agtgtccttt 240 ctccttactg gcaccaggtc aagttcctaa cgagtcttca gaggaggcag caggaagctc 300 agagagctgc aaagcaaccg tgcccatctg tcaagacatt cctgagaaga acatacaaga 360 aagtcttcct caaagaaaaa ccagtcggag ccgagtctat cttcacactt tggcagagag 420 tatttgcaaa ctgattttcc cagagtttga acggctgaat gttgcacttc agagaacatt 480 ggcaaagcac aaaataaaag aaagcaggaa atctttggaa agagaagact ttgaaaaaac 540 aattgcagag caagcagttg cagcaggagt tccagtggag gttatcaaag aatctcttgg 600 tgaagaggtt tttaaaatat gttacgagga agatgaaaac atccttgggg tggttggagg 660 cacccttaaa gattttttaa acagcttcag tacccttctg aaacagagca gccattgcca 720 agaagcagga aaaaggggca ggcttgagga cgcctccatt ctatgcctgg ataaggagga 780 tgattttcta catgtttact acttcttccc taagagaacc acctccctga ttcttcccgg 840 catcataaag gcagctgctc acgtattata tgaaacggaa gtggaagtgt cgttaatgcc 900 tccctgcttc cataatgatt gcagcgagtt tgtgaatcag ccctacttgt tgtactccgt 960 tcacatgaaa agcaccaagc catccctgtc ccccagcaaa ccccagtcct cgctggtgat 1020 tcccacatcg ctattctgca agacatttcc attccatttc atgtttgaca aagatatgac 1080 aattctgcaa tttggcaatg gcatcagaag gctgatgaac aggagagact ttcaaggaaa 1140 gcctaatttt gaagaatact ttgaaattct gactccaaaa atcaaccaga cgtttagcgg 1200 gatcatgact atgttgaata tgcagtttgt tgtacgagtg aggagatggg acaactctgt 1260 gaagaaatct tcaagggtta tggacctcaa aggccaaatg atctacattg ttgaatccag 1320 tgcaatcttg tttttggggt caccctgtgt ggacagatta gaagatttta caggacgagg 1380 gctctacctc tcagacatcc caattcacaa tgcactgagg gatgtggtct taatagggga 1440 acaagcccga gctcaagatg gcctgaagaa gaggctgggg aagctgaagg ctacccttga 1500 gcaagcccac caagccctgg aggaggagaa gaaaaagaca gtagaccttc tgtgctccat 1560 atttccctgt gaggttgctc agcagctgtg gcaagggcaa gttgtgcaag ccaagaagtt 1620 cagtaatgtc accatgctct tctcagacat cgttgggttc actgccatct gctcccagtg 1680 ctcaccgctg caggtcatca ccatgctcaa tgcactgtac actcgcttcg accagcagtg 1740 tggagagctg gatgtctaca aggtggagac cattggcgat gcctattgtg tagctggggg 1800 attacacaaa gagagtgata ctcatgctgt tcagatagcg ctgatggccc tgaagatgat 1860 ggagctctct gatgaagtta tgtctcccca tggagaacct atcaagatgc gaattggact 1920 gcactctgga tcagtttttg ctggcgtcgt tggagttaaa atgccccgtt actgtctttt 1980 tggaaacaat gtcactctgg ctaacaaatt tgagtcctgc agtgtaccac gaaaaatcaa 2040 tgtcagccca acaacttaca gattactcaa agactgtcct ggtttcgtgt ttacccctcg 2100 atcaagggag gaacttccac caaacttccc tagtgaaatc cccggaatct gccattttct 2160 ggatgcttac caacaaggaa caaactcaaa accatgcttc caaaagaaag atgtggaaga 2220 tggcaatgcc aattttttag gcaaagcatc aggaatagat tagcaacaag ggcaattcga 2280 agcttaggcc tggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg 2340 gtcatcatca ccatcaccat tgacgtctct agcttggagt cgacaacttg tttattgcag 2400 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 2460 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat ctctcgactc 2520 tgctgaagag gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa aggagtttgc 2580 accagacgca cctctgttca ctggtccggc gtattaaaac acgatacatt gttattagta 2640 catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt 2700 taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt 2760 ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt 2820 tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt 2880 ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt 2940 cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt 3000 atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaataaagc 3060 ctggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca 3120 accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt 3180 aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattatttc 3240 tgattgcggg cgtttttggg cgggtttcaa tctaactgtg cccgatttta attcagacaa 3300 cacgttagaa agcgatggtg caggcggtgg taacatttca gacggcaaat ctactaatgg 3360 cggcggtggt ggagctgatg ataaatctac catcggtgga ggcgcaggcg gggctggcgg 3420 cggaggcgga ggcggaggtg gtggcggtga tgcagacggc ggtttaggct caaatgtctc 3480 tttaggcaac acagtcggca cctcaactat tgtactggtt tcgggcgatg gtgcactctc 3540 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct 3600 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc 3660 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag 3720 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg 3780 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 3840 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 3900 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 3960 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 4020 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 4080 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 4140 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 4200 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 4260 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 4320 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 4380 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 4440 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 4500 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 4560 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 4620 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 4680 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 4740 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 4800 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 4860 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 4920 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 4980 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 5040 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 5100 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 5160 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 5220 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 5280 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 5340 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 5400 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 5460 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 5520 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 5580 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 5640 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 5700 gaggaagcat cctgcaccat cgtctgctca tccatgacct gaccatgcag aggatgatgc 5760 tcgtgacggt taacgcctcg aatcagcaac ggcttgccgt tcagcagcag cagaccattt 5820 tcaatccgca cctcgcggaa accgacatcg caggcttctg cttcaatcag cgtgccgtcg 5880 gcggtgtgca gttcaaccac cgcacgatag agattcggga tttcggcgct ccacagtttc 5940 gggttttcga cgttcagacg tagtgtgacg cgatcggcat aaccaccacg ctcatcgata 6000 atttcaccgc cgaaaggcgc ggtgccgctg gcgacctgcg tttcaccctg ccataaagaa 6060 actgttaccc gtaggtagtc acgcaactcg ccgcacatct gaacttcagc ctccagtaca 6120 gcgcggctga aatcatcatt aaagcgagtg gcaacatgga aatcgctgat ttgtgtagtc 6180 ggtttatgca gcaacgagac gtcacggaaa atgccgctca tccgccacat atcctgatct 6240 tccagataac tgccgtcact ccagcgcagc accatcaccg cgaggcggtt ttctccggcg 6300 cgtaaaaatg cgctcaggtc aaattcagac ggcaaacgac tgtcctggcc gtaaccgacc 6360 cagcgcccgt tgcaccacag atgaaacgcc gagttaacgc catcaaaaat aattcgcgtc 6420 tggccttcct gtagccagct ttcatcaaca ttaaatgtga gcgagtaaca acccgtcgga 6480 ttctccgtgg gaacaaacgg cggattgacc gtaatgggat aggtcacgtt ggtgtagatg 6540 ggcgcatcgt aaccgtgcat ctgccagttt gaggggacga cgacagtatc ggcctcagga 6600 agatcgcact ccagccagct ttccggcacc gcttctggtg ccggaaacca ggcaaagcgc 6660 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta 6720 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg 6780 ttttcccagt cacgacgttg taaaacgacg ggatctatca tttttagcag tgattctaat 6840 tgcagctgct ctttgataca actaatttta cgacgacgat gcgagctttt attcaaccga 6900 gcgtgcatgt ttgcaatcgt gcaagcgtta tcaatttttc attatcgtat tgttgcacat 6960 caacaggctg gacaccacgt tgaactcgcc gcagttttgc ggcaagttgg acccgccgcg 7020 catccaatgc aaactttccg acattctgtt gcctacgaac gattgattct ttgtccattg 7080 atcgaagcga gtgccttcga ctttttcgtg tccagtgtgg ctt 7123 //