ID pdcDNA-HA standard; circular DNA; ; 7173 BP. DE LMBP 4536. CC Accession number: LMBP 4536 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 28-OCT-2011 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 3878..4672 FT /vntifkey="4" FT /label=Tn5\neomycin\resistance FT CDS complement(6177..7037) FT /vntifkey="4" FT /label=ampicillin\resistance FT attenuator 2567..2691 FT /vntifkey="0" FT /label=attR2 FT attenuator complement(987..1111) FT /vntifkey="0" FT /label=attR1 FT promoter 863..879 FT /vntifkey="30" FT /label=T7\promoter FT promoter complement(2728..2744) FT /vntifkey="30" FT /label=SP6\promoter FT terminator 4846..4920 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(4846..4920) FT /vntifkey="43" FT /label=SV40\polyA\late FT CDS 1220..1879 FT /vntifkey="4" FT /label=chloramphenicol\resistance FT CDS 2221..2526 FT /vntifkey="4" FT /label=ccdB FT terminator 2770..2994 FT /vntifkey="43" FT /label=BGH\polyA FT rep_origin complement(5234..5636) FT /vntifkey="33" FT /label=pMB1\ori FT promoter 3473..3754 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 3040..3468 FT /vntifkey="33" FT /label=f1\ori FT rep_origin 3711..3786 FT /vntifkey="33" FT /label=SV40\ori FT misc_marker 957..983 FT /vntifkey="22" FT /label=HA\epitope FT promoter complement(5059..5142) FT /vntifkey="30" FT /label=lac\promoter FT promoter 235..818 FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT promoter 1119..1171 FT /vntifkey="30" FT /label=lacUV5\promoter SQ Sequence 7173 BP; 1742 A; 1775 C; 1791 G; 1865 t; gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 900 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atcatgtacc 960 catacgacgt cccagactac gctatcacaa gtttgtacaa aaaagctgaa cgagaaacgt 1020 aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat 1080 actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac 1140 actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgg cgagattttc 1200 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc 1260 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa 1320 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa 1380 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg 1440 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt 1500 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg 1560 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt 1620 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac 1680 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg 1740 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc 1800 cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga 1860 gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta aaagccagat aacagtatgc 1920 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat 1980 gtcaaaaaga ggtgtgctat gaagcagcgt attacagtga cagttgacag cgacagctat 2040 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga 2100 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg 2160 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg gactggtgaa 2220 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta 2280 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt 2340 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc 2400 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg 2460 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga 2520 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat 2580 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt 2640 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg tgattcccct 2700 cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat 2760 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 2820 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 2880 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 2940 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg 3000 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat 3060 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 3120 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 3180 aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc 3240 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 3300 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 3360 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg 3420 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa 3480 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 3540 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 3600 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 3660 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 3720 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg 3780 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt 3840 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 3900 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 3960 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 4020 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc 4080 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 4140 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 4200 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 4260 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 4320 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 4380 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca 4440 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 4500 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 4560 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 4620 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact 4680 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc 4740 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg 4800 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca 4860 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 4920 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata 4980 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 5040 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 5100 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 5160 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 5220 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 5280 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 5340 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 5400 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 5460 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 5520 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 5580 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg 5640 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 5700 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 5760 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 5820 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 5880 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 5940 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 6000 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 6060 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 6120 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 6180 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 6240 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 6300 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 6360 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 6420 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 6480 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 6540 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 6600 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 6660 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 6720 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 6780 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 6840 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 6900 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 6960 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 7020 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 7080 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 7140 cgcacatttc cccgaaaagt gccacctgac gtc 7173 //